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The map label for this gene is ruvA

Identifier: 94309369

GI number: 94309369

Start: 448795

End: 449376

Strand: Reverse

Name: ruvA

Synonym: Rmet_0424

Alternate gene names: 94309369

Gene position: 449376-448795 (Counterclockwise)

Preceding gene: 94309370

Following gene: 94309368

Centisome position: 11.44

GC content: 64.95

Gene sequence:

>582_bases
ATGATCGGACGTATCGCTGGCACCCTGCTCGAAAAGAATCCCCCGCATCTGCTGGTCGACTGCCATGGCGTCGGCTACGA
GATCGATGTGCCGATGAGCACGTTCTACAACCTGCCGGCGATCGGCCATCCCGTAACGCTGCTGACCCAGCAGATCGTGC
GGGAGGACGCGCACCTGCTCTTCGGCTTTGCCACGGCCACCGAACGCAATACATTCCGGGAGCTGATCAAGATCACCGGC
GTCGGCGCGCGCATGGCGCTGGCCGTGCTGTCTGGGCTGTCGGTGCCGGAACTGGCGCAGGCCGTGACGATGCAGGAAGC
GGGCCGACTGACCAAGATCCCGGGCATCGGCAAAAAGACAGCAGAGCGGCTGCTACTGGAGCTCAAGGGCAAACTCGGCG
CGGATCTTGGCCATGCTCCCGGCGCCACACCGCTGGCCGATAGCGCCGTCGACATCCTCAACGCGCTGCTCGCGCTCGGC
TACTCGGAGAAGGAGGCCGCGCAGGCCATCAAGCAGGTGCCGGCCGGCACTGGCGTGTCCGACGGTATCAAGCTTGCGCT
GAAGGCGCTGTCGAAAGGCTGA

Upstream 100 bases:

>100_bases
GCTGGGTCGCCGCGCCGTGGATCCCCCCGCCAACGGCGGCCATTAAGCACGCTGGCCGCGCTACACTTGCGGCCAATTTC
GCTTCTCCATCGCACCCACC

Downstream 100 bases:

>100_bases
GGACGCATCGCGAACGGCTCGGGGCGGGGGGGCGCAGCGTAGAATGGCGGCAAATTGCTTCTGACTGCCATGATTGAAAC
CGACAAGCTGACCGGCGCCG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MIGRIAGTLLEKNPPHLLVDCHGVGYEIDVPMSTFYNLPAIGHPVTLLTQQIVREDAHLLFGFATATERNTFRELIKITG
VGARMALAVLSGLSVPELAQAVTMQEAGRLTKIPGIGKKTAERLLLELKGKLGADLGHAPGATPLADSAVDILNALLALG
YSEKEAAQAIKQVPAGTGVSDGIKLALKALSKG

Sequences:

>Translated_193_residues
MIGRIAGTLLEKNPPHLLVDCHGVGYEIDVPMSTFYNLPAIGHPVTLLTQQIVREDAHLLFGFATATERNTFRELIKITG
VGARMALAVLSGLSVPELAQAVTMQEAGRLTKIPGIGKKTAERLLLELKGKLGADLGHAPGATPLADSAVDILNALLALG
YSEKEAAQAIKQVPAGTGVSDGIKLALKALSKG
>Mature_193_residues
MIGRIAGTLLEKNPPHLLVDCHGVGYEIDVPMSTFYNLPAIGHPVTLLTQQIVREDAHLLFGFATATERNTFRELIKITG
VGARMALAVLSGLSVPELAQAVTMQEAGRLTKIPGIGKKTAERLLLELKGKLGADLGHAPGATPLADSAVDILNALLALG
YSEKEAAQAIKQVPAGTGVSDGIKLALKALSKG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=43.7810945273632, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_RALME (Q1LRB6)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582579.1
- ProteinModelPortal:   Q1LRB6
- SMR:   Q1LRB6
- STRING:   Q1LRB6
- GeneID:   4037212
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0424
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- PhylomeDB:   Q1LRB6
- ProtClustDB:   PRK00116
- BioCyc:   RMET266264:RMET_0424-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20192; Mature: 20192

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRIAGTLLEKNPPHLLVDCHGVGYEIDVPMSTFYNLPAIGHPVTLLTQQIVREDAHLL
CCCHHHHHHHCCCCCEEEEEECCCCEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FGFATATERNTFRELIKITGVGARMALAVLSGLSVPELAQAVTMQEAGRLTKIPGIGKKT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
AERLLLELKGKLGADLGHAPGATPLADSAVDILNALLALGYSEKEAAQAIKQVPAGTGVS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCH
DGIKLALKALSKG
HHHHHHHHHHHCC
>Mature Secondary Structure
MIGRIAGTLLEKNPPHLLVDCHGVGYEIDVPMSTFYNLPAIGHPVTLLTQQIVREDAHLL
CCCHHHHHHHCCCCCEEEEEECCCCEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FGFATATERNTFRELIKITGVGARMALAVLSGLSVPELAQAVTMQEAGRLTKIPGIGKKT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
AERLLLELKGKLGADLGHAPGATPLADSAVDILNALLALGYSEKEAAQAIKQVPAGTGVS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCH
DGIKLALKALSKG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA