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The map label for this gene is ruvB

Identifier: 94309368

GI number: 94309368

Start: 447658

End: 448725

Strand: Reverse

Name: ruvB

Synonym: Rmet_0423

Alternate gene names: 94309368

Gene position: 448725-447658 (Counterclockwise)

Preceding gene: 94309369

Following gene: 94309367

Centisome position: 11.42

GC content: 66.48

Gene sequence:

>1068_bases
ATGATTGAAACCGACAAGCTGACCGGCGCCGACCGCCTGCCCGACCGCGTGATCTCGGCCACGCCCGCTTCCACGCAGGA
AGAAGCGTTCGAGCGGGCGCTACGGCCCAAGCTGCTCGACGAGTACGTCGGTCAGGAGAAGGTGCGCGGCCAGCTCGACA
TCTTCATGCACGCGGCCCGCAAGCGCCGCGAGGCACTGGACCACGTGCTGCTGTTCGGGCCGCCCGGCTTGGGCAAGACC
ACGCTGGCCCACATCATCGCGCGCGAGATGGGCGTCAATCTGCGCCAGACATCGGGGCCGGTGCTCGAGCGCCCGGGCGA
TCTGGCCGCCCTGCTGACCAACCTCGAAGCCCACGACGTCCTCTTCATCGACGAAATTCACCGTTTGTCGCCGGTGGTCG
AGGAAATCCTGTACCCGGCGCTGGAGGACTACCAGATCGACATCATGATCGGCGAAGGTCCGGCGGCCCGCTCGGTCAAG
CTCGACCTGCAACCCTTCACGCTGGTGGGCGCCACCACGCGCGCGGGGATGCTGACCAATCCGCTGCGCGACCGTTTCGG
CATCGTGGCGCGGCTCGAGTTCTACACCCCGGACGAGCTCGCACGCATCGTCGCTCGCTCGGCGCTGTTGCTGAATGCCG
CCATTGACCCGGCCGGCGCATTGGAAATTGCCCGCCGCGCGCGCGGCACGCCCCGGATCGCCAATCGGCTGCTACGTCGC
GTGCGTGACTTTGCTGAAGTGAAAAGCGACGGAACGATCACTAGAGAGCTGGCCGATGCCGCACTGGAGATGCTCGACGT
GGATAGCGTTGGCTTCGACCTGATGGACCGCAAGCTGCTGGAAGCCGTGTTGCATAAATTCGACGGTGGCCCGGTCGGCG
TGGACAATCTGGCCGCAGCGATCGGCGAAGCCCGCGACACGATCGAGGACGTGCTGGAGCCCTACATGATCCAACAGGGC
TACCTGCAGCGGACGCCGCGAGGACGTATGGCGACAGCCGCCGCCTACCGCCATTTCGGGCTGGCATCGCCCCGCGACGG
TGGCGCCGATCTCGCCGAAGGGCTCTGA

Upstream 100 bases:

>100_bases
GCTTGCGCTGAAGGCGCTGTCGAAAGGCTGAGGACGCATCGCGAACGGCTCGGGGCGGGGGGGCGCAGCGTAGAATGGCG
GCAAATTGCTTCTGACTGCC

Downstream 100 bases:

>100_bases
TTTTCCGCATCCCGCCCAAGGCCGTTCCACGGCCTTTTTTTTGCGCCTCCAGATGCCCCGGCGACCGCCAGGCGCGCCAT
TTAAACGTTTTAGCACCCCG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAARKRREALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRR
VRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAAIGEARDTIEDVLEPYMIQQG
YLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL

Sequences:

>Translated_355_residues
MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAARKRREALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRR
VRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAAIGEARDTIEDVLEPYMIQQG
YLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL
>Mature_355_residues
MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAARKRREALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRR
VRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAAIGEARDTIEDVLEPYMIQQG
YLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=72.7272727272727, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_RALME (Q1LRB7)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582578.1
- ProteinModelPortal:   Q1LRB7
- SMR:   Q1LRB7
- STRING:   Q1LRB7
- GeneID:   4037211
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0423
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q1LRB7
- ProtClustDB:   PRK00080
- BioCyc:   RMET266264:RMET_0423-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38967; Mature: 38967

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAAR
CCCCCCCCCCHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KRREALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDV
HHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHCC
LFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQPFTLVGATTRAGMLTN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHHH
PLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRR
HHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
VRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAA
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IGEARDTIEDVLEPYMIQQGYLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAAR
CCCCCCCCCCHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KRREALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDV
HHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHCC
LFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQPFTLVGATTRAGMLTN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHHH
PLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRR
HHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
VRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAA
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IGEARDTIEDVLEPYMIQQGYLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA