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The map label for this gene is aspS [H]

Identifier: 94309323

GI number: 94309323

Start: 403176

End: 404984

Strand: Direct

Name: aspS [H]

Synonym: Rmet_0378

Alternate gene names: 94309323

Gene position: 403176-404984 (Clockwise)

Preceding gene: 94309322

Following gene: 94309324

Centisome position: 10.26

GC content: 63.74

Gene sequence:

>1809_bases
ATGTCCTCCATGCGTACTCACTACTGCGGTCTGGTGACCGAACAACTGATCGGCCAGGAAGTGGCCCTGACCGGCTGGGT
GCAGCGCCGCCGCGATCACGGTGGCGTGATCTTCATCGACCTGCGCGATCGCGAGGGCCTGGTGCAGGTGGTGTGCGACC
CGGATCGTCCGGAGATGTTCAAGGCCGCGGAAGAAATCCGCAACGAGTTCTGCATCCGCATCACCGGCAAGGTGCGCCCG
CGTCCGGCCGGCACCGAGAACGCCAACCTGACCTCGGGCAAGATCGAGGTGCTGTGCCATGAGCTGACCGTGCTGAACCC
GTCGGTCACGCCTCCGTTCCAGCTCGATGACGACAACCTGTCGGAAACCACCCGCCTGACGCACCGCGTGCTGGATCTGC
GCCGTCCGCAGATGCAATACAACCTGCGCCTGCGCTACAAGGTGGCGATGGAAGTGCGCAAGTACCTGGACGCACAGGGC
TTCATCGACATCGAGACGCCGATGCTCGGCAAGAGCACGCCCGAAGGCGCCCGCGACTATCTCGTGCCGTCGCGCGTGAA
CCCGGGTCACTTCTTCGCGCTGCCGCAGTCGCCGCAGATCTTCAAGCAGATGCTGATGGTCTCGGGCTTCGACCGTTACT
ACCAGATCACCAAGTGCTTCCGTGACGAGGACCTGCGCGCCGACCGCCAGCCCGAATTCACGCAGATCGACTGCGAAACG
TCGTTCCTGACCGAACAGGAAATCCGTGACCTGTTCGAGGACATGATGCGCACCGTGTTCAAGAACGCGATCGACGTGGA
CCTGGACGCCAAGTTCCCGGTGATGGAGTTCCGCGAGGCGATGGCCCGCTTCGGCTCGGACAAGCCGGACCTGCGCGTCA
AGCTCGAATTCACCGAACTGACCGAGGTGATGAAGGACGTCGACTTCAAGGTGTTCTCGGGCCCGGCCAACAGCGACAAC
GGTCGCGTGGTCGGCCTGCGCGTGCCGGGTGGCGGCGCGATCTCGCGTGGCGAAATCGATGCCTACACGCAGTTCGTGGC
GATCTATGGCGCCAAGGGCCTGGCGTGGATCAAGGTCAATGAAGTGGCCAAGGGCCGCGACGGCCTGCAATCGCCGATCG
TGAAGAACCTGCATGACGCCGCGATTGCCGAGATCCTGAAGCGCACTGGCGCCCAGGACGGCGACATCATCTTCTTCGGC
GCCGACAAGGCCAAGATCGTCAACGACGCGATCGGCGCGCTGCGCCTGAAGGTCGGCCATTCGGAGTTCGGCAAGAGCAC
GGGCCTGTTCGAAGACACCTGGAAGCCGCTGTGGGTGATCGACTTCCCGATGTTCGAGTACGACGAGGAAGATGCCCGCT
GGGTGGCCATGCACCACCCGTTCACGAGCCCGAAGGACGAGCACCTGCAGTACCTGGAAACCGATCCGGGCAAGTGCATC
GCCAAGGCCTACGACATGGTGCTGAACGGCTGGGAAATGGGCGGCGGCTCGGTGCGTATCTACCGCGAGGACGTGCAGAG
CAAGGTGTTCCGCGCCCTGAAGATCAACGACGAGGAAGCTCGCGCCAAGTTCGGTTACCTGCTCGACGCGCTGCAGTACG
GCGCGCCGCCGCACGGTGGCCTGGCCTTCGGCCTGGACCGTATCGTGACGATGATGGCCGGCGCTGACTCGATCCGTGAC
GTGATCGCGTTCCCGAAGACGCAACGTGCGCAGGATCTGCTGACGCAGGCCCCGAGCACGGTCGACGAGAAGCAGTTGCG
AGAACTGCACATTCGTCTGCGTGCCCAGGAGCCGAAGACCACGGCCTGA

Upstream 100 bases:

>100_bases
AGTGGCCGATGCCGATGCCGACGACGCCGTAGCCGATACTCATACCAATTGATTGCGCGCGGCCGTTCATCGCGGCCGCG
CATTACAGGGAATCTCTCCT

Downstream 100 bases:

>100_bases
TCGTTGTTCAGACCCGCAAAAAAGCCGCGCCGATGCGCGGCTTTTTTGTTCTTGGTGCAAGGATGCGTTTCCATTTGTTA
CTACCCGGAACGAAGCCGCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSSMRTHYCGLVTEQLIGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEFCIRITGKVRP
RPAGTENANLTSGKIEVLCHELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQG
FIDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGPANSDN
GRVVGLRVPGGGAISRGEIDAYTQFVAIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRTGAQDGDIIFFG
ADKAKIVNDAIGALRLKVGHSEFGKSTGLFEDTWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKDEHLQYLETDPGKCI
AKAYDMVLNGWEMGGGSVRIYREDVQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRD
VIAFPKTQRAQDLLTQAPSTVDEKQLRELHIRLRAQEPKTTA

Sequences:

>Translated_602_residues
MSSMRTHYCGLVTEQLIGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEFCIRITGKVRP
RPAGTENANLTSGKIEVLCHELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQG
FIDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGPANSDN
GRVVGLRVPGGGAISRGEIDAYTQFVAIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRTGAQDGDIIFFG
ADKAKIVNDAIGALRLKVGHSEFGKSTGLFEDTWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKDEHLQYLETDPGKCI
AKAYDMVLNGWEMGGGSVRIYREDVQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRD
VIAFPKTQRAQDLLTQAPSTVDEKQLRELHIRLRAQEPKTTA
>Mature_601_residues
SSMRTHYCGLVTEQLIGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEFCIRITGKVRPR
PAGTENANLTSGKIEVLCHELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGF
IDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTEVMKDVDFKVFSGPANSDNG
RVVGLRVPGGGAISRGEIDAYTQFVAIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRTGAQDGDIIFFGA
DKAKIVNDAIGALRLKVGHSEFGKSTGLFEDTWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKDEHLQYLETDPGKCIA
KAYDMVLNGWEMGGGSVRIYREDVQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSTVDEKQLRELHIRLRAQEPKTTA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=38.2059800664452, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=261, Percent_Identity=28.735632183908, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI5031815, Length=254, Percent_Identity=25.9842519685039, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI194272210, Length=247, Percent_Identity=25.1012145748988, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=58.3756345177665, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=24.5791245791246, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790571, Length=286, Percent_Identity=24.1258741258741, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=609, Percent_Identity=33.3333333333333, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=269, Percent_Identity=22.3048327137546, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=269, Percent_Identity=22.3048327137546, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=269, Percent_Identity=22.3048327137546, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=25.7462686567164, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=33.0708661417323, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=290, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324256, Length=183, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=266, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=36.3036303630363, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=28.6792452830189, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640849, Length=259, Percent_Identity=26.2548262548263, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640851, Length=259, Percent_Identity=26.2548262548263, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68187; Mature: 68056

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMRTHYCGLVTEQLIGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMF
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
KAAEEIRNEFCIRITGKVRPRPAGTENANLTSGKIEVLCHELTVLNPSVTPPFQLDDDNL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEEHEEECCCCCCCCEECCCCC
SETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGFIDIETPMLGKSTPEGARDY
HHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTEL
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHH
TEVMKDVDFKVFSGPANSDNGRVVGLRVPGGGAISRGEIDAYTQFVAIYGAKGLAWIKVN
HHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHEECCCCEEEEEEC
EVAKGRDGLQSPIVKNLHDAAIAEILKRTGAQDGDIIFFGADKAKIVNDAIGALRLKVGH
CHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHEEEEECC
SEFGKSTGLFEDTWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKDEHLQYLETDPGKCI
HHHCCCCCCCHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
AKAYDMVLNGWEMGGGSVRIYREDVQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGG
HHHHHHHHCCEECCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSTVDEKQLRELHIRLRAQEPKT
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
TA
CC
>Mature Secondary Structure 
SSMRTHYCGLVTEQLIGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMF
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
KAAEEIRNEFCIRITGKVRPRPAGTENANLTSGKIEVLCHELTVLNPSVTPPFQLDDDNL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEEHEEECCCCCCCCEECCCCC
SETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGFIDIETPMLGKSTPEGARDY
HHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTEL
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHH
TEVMKDVDFKVFSGPANSDNGRVVGLRVPGGGAISRGEIDAYTQFVAIYGAKGLAWIKVN
HHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHEECCCCEEEEEEC
EVAKGRDGLQSPIVKNLHDAAIAEILKRTGAQDGDIIFFGADKAKIVNDAIGALRLKVGH
CHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHEEEEECC
SEFGKSTGLFEDTWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKDEHLQYLETDPGKCI
HHHCCCCCCCHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
AKAYDMVLNGWEMGGGSVRIYREDVQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGG
HHHHHHHHCCEECCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSTVDEKQLRELHIRLRAQEPKT
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
TA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA