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The map label for this gene is pfpI [H]

Identifier: 94309255

GI number: 94309255

Start: 325964

End: 326593

Strand: Reverse

Name: pfpI [H]

Synonym: Rmet_0310

Alternate gene names: 94309255

Gene position: 326593-325964 (Counterclockwise)

Preceding gene: 94309258

Following gene: 94309254

Centisome position: 8.31

GC content: 65.56

Gene sequence:

>630_bases
ATGGCCCGGCAGTTTGAACATCCGCAACCCGCAACGATACAGGAGACAACCGTGGCCAAGAAGATCTTGATGCTGGCTGG
CGACTTCGCCGAAGACTACGAAACCATGGTGCCGTTCCAGGCACTGCAGATGGTGGGCCACACGGTTCACGCGGTCTGCC
CGGACAAGAAGGCTGGCGACCACTGCGCCACCGCCATCCATGACTTCGAAGGCGACCAGACCTACACGGAGAAGCGCGGC
CACAACTTTACGCTGAATGCCACGTTCGCGGACATCGATCCGGCGCGGTATGACGCCCTCGTCATTCCAGGCGGCCGCGC
CCCCGAATACCTGCGGCTGAATGCCCGCGTGCTTGAGATCGTGCGCCACTTCGCCCAGGCCAACAAGCCGATCGCCGCGG
TCTGCCACGGCGCGCAACTGCTGGCGGCAGCCGGCGTGCTCGAAGGTCGCACCTGTTCCGCCTACCCCGCCTGTGCGCCC
GAGGTGCGGCTGGCCGGCGGCAAATATGCCGATATCCCGGTCGACCAGGCCCATACCGACGGCAACCTGGTAACGGCGCC
GGCCTGGCCTGCCCATCCGGCCTGGCTGGCGCAGTTCCTTGCAGTGCTCGGGACGCGGATCGTAGAGTAG

Upstream 100 bases:

>100_bases
GGGACTGGATGATGGAGTTCGACATGAAAGAAACTGGAAAGTGAAATTACGCGCGATGCGGTAACGGGCTACCACCCGTG
TCCAAAACCATCGCCGCACA

Downstream 100 bases:

>100_bases
AGGCTTCCGAACGCGACGCGGGCCATCGGGCCCGCGGCCGCGCCGCACGGAGGTATCCATGTGTGAGATCTTCATCCGGG
CCAATCCGGATTCGTACCAG

Product: putative intracellular protease/amidase/DJ-1/PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MARQFEHPQPATIQETTVAKKILMLAGDFAEDYETMVPFQALQMVGHTVHAVCPDKKAGDHCATAIHDFEGDQTYTEKRG
HNFTLNATFADIDPARYDALVIPGGRAPEYLRLNARVLEIVRHFAQANKPIAAVCHGAQLLAAAGVLEGRTCSAYPACAP
EVRLAGGKYADIPVDQAHTDGNLVTAPAWPAHPAWLAQFLAVLGTRIVE

Sequences:

>Translated_209_residues
MARQFEHPQPATIQETTVAKKILMLAGDFAEDYETMVPFQALQMVGHTVHAVCPDKKAGDHCATAIHDFEGDQTYTEKRG
HNFTLNATFADIDPARYDALVIPGGRAPEYLRLNARVLEIVRHFAQANKPIAAVCHGAQLLAAAGVLEGRTCSAYPACAP
EVRLAGGKYADIPVDQAHTDGNLVTAPAWPAHPAWLAQFLAVLGTRIVE
>Mature_208_residues
ARQFEHPQPATIQETTVAKKILMLAGDFAEDYETMVPFQALQMVGHTVHAVCPDKKAGDHCATAIHDFEGDQTYTEKRGH
NFTLNATFADIDPARYDALVIPGGRAPEYLRLNARVLEIVRHFAQANKPIAAVCHGAQLLAAAGVLEGRTCSAYPACAPE
VRLAGGKYADIPVDQAHTDGNLVTAPAWPAHPAWLAQFLAVLGTRIVE

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=190, Percent_Identity=32.6315789473684, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 22607; Mature: 22476

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQFEHPQPATIQETTVAKKILMLAGDFAEDYETMVPFQALQMVGHTVHAVCPDKKAGD
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HCATAIHDFEGDQTYTEKRGHNFTLNATFADIDPARYDALVIPGGRAPEYLRLNARVLEI
HHHHHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCHHEEHHHHHHHH
VRHFAQANKPIAAVCHGAQLLAAAGVLEGRTCSAYPACAPEVRLAGGKYADIPVDQAHTD
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
GNLVTAPAWPAHPAWLAQFLAVLGTRIVE
CCEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARQFEHPQPATIQETTVAKKILMLAGDFAEDYETMVPFQALQMVGHTVHAVCPDKKAGD
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
HCATAIHDFEGDQTYTEKRGHNFTLNATFADIDPARYDALVIPGGRAPEYLRLNARVLEI
HHHHHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCCCCHHEEHHHHHHHH
VRHFAQANKPIAAVCHGAQLLAAAGVLEGRTCSAYPACAPEVRLAGGKYADIPVDQAHTD
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
GNLVTAPAWPAHPAWLAQFLAVLGTRIVE
CCEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]