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The map label for this gene is rsmD [H]

Identifier: 94309229

GI number: 94309229

Start: 301238

End: 301870

Strand: Direct

Name: rsmD [H]

Synonym: Rmet_0284

Alternate gene names: 94309229

Gene position: 301238-301870 (Clockwise)

Preceding gene: 94309228

Following gene: 94309230

Centisome position: 7.67

GC content: 67.61

Gene sequence:

>633_bases
ATGAATTCGCAATCCCGACCTTCGCCCGCACCGCGCGGGCGAACTTCTGTTCCCCGCAAGTCCCCCACCCAGGTGCGCAT
CATCGGCGGGCGCTGGAAGCGATCGCTGCTGCCGGTGCTCGAAGCCGAAGGGCTTCGTCCGACACCTGACCGTGTGCGCG
AAACGTTGTTCAACTGGCTTGGCCAGGACCTGTCCGGCCTGACCTGCCTCGACCTGTTCGCCGGTTCGGGCGCGCTCGGC
TTCGAGGCAGCGTCCCGGGGGGCCAGTGCCGTGACGCTGGTCGAAGCCAACCCGCACGTGGTGCGTCAGCTCCGCGACAA
CCAGTACCGGCTCGATGCCTCGCAGGTGAAGATCATCCAAAGCGACGCCTTCGCGGCAGCCGCGCAGATGCCGGCGGCCA
GCTTCGACGTGGTATTCCTGGACCCGCCCTTTGCCGAAGACTGGCTCGGGCCGGCGCTTGAACACGCGGCGCGTCTGTCA
CGTCCGGGGGGAGCCATCTATGTCGAAACGGATCGTGCGCTGATCGGCCCCGATGCGCCGATACCGGCGTCCCTGGAGAT
CGTTCGCCAGGCACGCGCGGGGGCGGTGCATTTCCATCTGCTGCAGCATCGCCTCGCCAACGGCGCTGGCTGA

Upstream 100 bases:

>100_bases
ACGTGATTTCGCGTGGCTTGGCACCCATGTGTGGGCCGGGCCACCCGCGGCGGCGACTGACCTTGCTGTGCCGGCGCGTG
GCAGGCTCTACAATCACGGC

Downstream 100 bases:

>100_bases
CCTGGGTCGTACGACCTCTTTGGTATGCCAGACAAGGGTTTGCGGGCCTGCTGCGGCGCGGCAGAAATGCGGCCTGATGG
ACTTCCAATTGCCCCGTTTG

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNSQSRPSPAPRGRTSVPRKSPTQVRIIGGRWKRSLLPVLEAEGLRPTPDRVRETLFNWLGQDLSGLTCLDLFAGSGALG
FEAASRGASAVTLVEANPHVVRQLRDNQYRLDASQVKIIQSDAFAAAAQMPAASFDVVFLDPPFAEDWLGPALEHAARLS
RPGGAIYVETDRALIGPDAPIPASLEIVRQARAGAVHFHLLQHRLANGAG

Sequences:

>Translated_210_residues
MNSQSRPSPAPRGRTSVPRKSPTQVRIIGGRWKRSLLPVLEAEGLRPTPDRVRETLFNWLGQDLSGLTCLDLFAGSGALG
FEAASRGASAVTLVEANPHVVRQLRDNQYRLDASQVKIIQSDAFAAAAQMPAASFDVVFLDPPFAEDWLGPALEHAARLS
RPGGAIYVETDRALIGPDAPIPASLEIVRQARAGAVHFHLLQHRLANGAG
>Mature_210_residues
MNSQSRPSPAPRGRTSVPRKSPTQVRIIGGRWKRSLLPVLEAEGLRPTPDRVRETLFNWLGQDLSGLTCLDLFAGSGALG
FEAASRGASAVTLVEANPHVVRQLRDNQYRLDASQVKIIQSDAFAAAAQMPAASFDVVFLDPPFAEDWLGPALEHAARLS
RPGGAIYVETDRALIGPDAPIPASLEIVRQARAGAVHFHLLQHRLANGAG

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=185, Percent_Identity=42.1621621621622, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22583; Mature: 22583

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQSRPSPAPRGRTSVPRKSPTQVRIIGGRWKRSLLPVLEAEGLRPTPDRVRETLFNWL
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
GQDLSGLTCLDLFAGSGALGFEAASRGASAVTLVEANPHVVRQLRDNQYRLDASQVKIIQ
CCCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHCCCEEECHHHHEEEH
SDAFAAAAQMPAASFDVVFLDPPFAEDWLGPALEHAARLSRPGGAIYVETDRALIGPDAP
HHHHHHHHHCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCCEEEEECCCEEECCCCC
IPASLEIVRQARAGAVHFHLLQHRLANGAG
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSQSRPSPAPRGRTSVPRKSPTQVRIIGGRWKRSLLPVLEAEGLRPTPDRVRETLFNWL
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
GQDLSGLTCLDLFAGSGALGFEAASRGASAVTLVEANPHVVRQLRDNQYRLDASQVKIIQ
CCCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHCCCEEECHHHHEEEH
SDAFAAAAQMPAASFDVVFLDPPFAEDWLGPALEHAARLSRPGGAIYVETDRALIGPDAP
HHHHHHHHHCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCCEEEEECCCEEECCCCC
IPASLEIVRQARAGAVHFHLLQHRLANGAG
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]