Click here to switch to the map view.

The map label for this gene is gpsA

Identifier: 94309200

GI number: 94309200

Start: 273233

End: 274261

Strand: Direct

Name: gpsA

Synonym: Rmet_0255

Alternate gene names: 94309200

Gene position: 273233-274261 (Clockwise)

Preceding gene: 94309199

Following gene: 94309201

Centisome position: 6.96

GC content: 68.22

Gene sequence:

>1029_bases
ATGAAACTGACCATTCTCGGCGCCGGCGCATGGGGTACCGCGCTTGCAAGCCACGCGGCCACGGCCGCCGCCGATCATGA
CGTGGTGCTCTGGGGGCGCGATCCGGCTCAGCTTGCCGGCATCGCCGCAACTGGCGTCAACGCCACCTATCTGCCGGGCG
TGAGCCTGTCGCCCCGGCTTGTCTGCGAGCCCGATTTCGATCGCGCCGTCGATCACGCCCTGACCGATCCGGAGGGGCTG
CTGATCATCGGCACGCCGGTATCCGGCCTGCGCGAGATGACGCGCAGGTTGGCCGCGCGTCGCAAAGGGCCGCTGGCGAT
GCTCTGGCTCTGCAAGGGCTTCGAGGCCGAGACCCATGCGATGCCGCACCAGATGGTGCACGATGAATTGAACGCGCTCG
GCGTGGCGCCGGGCGAGATCGAATATGGCGTGCTGACGGGGCCGAGCTTTGCCAAGGAAGTGGCGCAGGGTCTGCCGTGC
GCGCTGACCGTGGCCGGCACCGCGCACGTGCTGATCGATCGCGCGCAGGCGGCATTCCATCACCATGCCATGCGCATCTA
TGGCAGCGACGACCTGATCGGCGTCGAAGTCGGTGGCGCGGTGAAGAACGTGCTGGCGATCGCCACCGGTGCCAGCGATG
GCCTGGGGCTTGGGCTCAATGCGCGCGCGGCGCTGATCACGCGCGGGCTGGCCGAGATGACCCGCCTGGGCCTTGCGCTC
GGCGGTCGCGTCGAGACCTTCATGGGTCTGGCCGGCATGGGTGACCTGATCCTGACCGCCACCGGCGATTTGTCTCGTAA
CCGTAAGGTCGGCCAGCAACTGGCGGGCGGGAAGACGCTCGACCAGATCCTGGCAGACCTTGGCCATGTGGCCGAAGGCG
TGCGTTGCGCGCAGGCCGTTGCCGCGCTGGCGGCGCGCGTCGGTGTGGAAATGCCGATCACGCGCGCGGTATGCGCGGTG
CTGTTCGAAGGGTTGCCTGTTGCCGATGCGGTCTCGCAGTTGCTGCAGCGTGACGCGCGCGACGAGTAA

Upstream 100 bases:

>100_bases
ACACCAGAACGGGGCTGCGGCCCCGTTTGGTGTTTCTGCGAGGCGTCAACGTTCATACTGTCGCGACGACAGCGCTTTTC
GCTGACTTCACAGGCCTGCC

Downstream 100 bases:

>100_bases
CACTTGATGCCGAGTCGACGCCACACCCTCAAGGCCCTGATCGCCACCTTCGCAGCCGGCGCGGTTCCTGCCGTTGCCCA
TGCCGATACCTGGCCCGCCA

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRLVCEPDFDRAVDHALTDPEGL
LIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHAMPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPC
ALTVAGTAHVLIDRAQAAFHHHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLAL
GGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAVAALAARVGVEMPITRAVCAV
LFEGLPVADAVSQLLQRDARDE

Sequences:

>Translated_342_residues
MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRLVCEPDFDRAVDHALTDPEGL
LIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHAMPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPC
ALTVAGTAHVLIDRAQAAFHHHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLAL
GGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAVAALAARVGVEMPITRAVCAV
LFEGLPVADAVSQLLQRDARDE
>Mature_342_residues
MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRLVCEPDFDRAVDHALTDPEGL
LIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHAMPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPC
ALTVAGTAHVLIDRAQAAFHHHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLAL
GGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAVAALAARVGVEMPITRAVCAV
LFEGLPVADAVSQLLQRDARDE

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=360, Percent_Identity=30, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI24307999, Length=356, Percent_Identity=26.685393258427, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790037, Length=342, Percent_Identity=42.3976608187134, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32564399, Length=338, Percent_Identity=28.698224852071, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507425, Length=344, Percent_Identity=28.1976744186047, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564403, Length=346, Percent_Identity=28.0346820809249, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193210136, Length=346, Percent_Identity=28.0346820809249, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193210134, Length=188, Percent_Identity=35.6382978723404, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324513, Length=375, Percent_Identity=27.7333333333333, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320181, Length=375, Percent_Identity=27.4666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136204, Length=352, Percent_Identity=28.4090909090909, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136202, Length=352, Percent_Identity=28.4090909090909, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136200, Length=351, Percent_Identity=28.7749287749288, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI22026922, Length=364, Percent_Identity=24.7252747252747, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362270, Length=299, Percent_Identity=26.4214046822742, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45551945, Length=299, Percent_Identity=26.4214046822742, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648969, Length=258, Percent_Identity=26.3565891472868, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_RALME (Q1LRT5)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582410.1
- ProteinModelPortal:   Q1LRT5
- SMR:   Q1LRT5
- STRING:   Q1LRT5
- GeneID:   4037041
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0255
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   ALVACCK
- PhylomeDB:   Q1LRT5
- ProtClustDB:   PRK00094
- BioCyc:   RMET266264:RMET_0255-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 35365; Mature: 35365

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 202-202 BINDING 112-112 BINDING 112-112 BINDING 151-151 BINDING 266-266 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHEEECCCCCEECCCCCCCCEE
VCEPDFDRAVDHALTDPEGLLIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHA
EECCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHC
MPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPCALTVAGTAHVLIDRAQAAFH
CCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
HHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLAL
HHHEEEECCCCEEEEEECHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAV
CCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AALAARVGVEMPITRAVCAVLFEGLPVADAVSQLLQRDARDE
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHEEECCCCCEECCCCCCCCEE
VCEPDFDRAVDHALTDPEGLLIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHA
EECCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHC
MPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPCALTVAGTAHVLIDRAQAAFH
CCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
HHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLAL
HHHEEEECCCCEEEEEECHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAV
CCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AALAARVGVEMPITRAVCAVLFEGLPVADAVSQLLQRDARDE
HHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA