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The map label for this gene is ygfF [C]

Identifier: 94309127

GI number: 94309127

Start: 189069

End: 189869

Strand: Direct

Name: ygfF [C]

Synonym: Rmet_0182

Alternate gene names: 94309127

Gene position: 189069-189869 (Clockwise)

Preceding gene: 94309124

Following gene: 94309128

Centisome position: 4.81

GC content: 67.67

Gene sequence:

>801_bases
ATGGGCACTTCACAGGCTTCGAGCGACAAATCTCGCGGCGTAGCGCTGGTCACCGGCGCCGCACGCCGCCTGGGTCGTGC
CATCGCTCTCGAACTTGCCGCACAAGGCTGGGACGTTGCCGTTCACTGCAACCGCTCGCGCGACGAAGCGGACGCGCTGG
CCACCCAGATCCGCGGCCTCGGTCGCCGCGCCGCCGTGCTGCAGGCCGACCTGTCGGATGAAGCCGCCACGGCCCGTTTG
ATTCCCGCCTGCATCGACGCCCTTGGCGTGCCGGCCTGCCTGGTCAACAACGCGTCGCTGTTCCAGTACGACGTGGCGAC
GAGCTTCTCCTATGGGTCGCTCGACACGCACATGCGCACCAACGTGGCGGCCCCGCTGCTGCTGGCGCGTGAGATGCACA
AGGCGCTTGGCGCCGACGGCAACGAGACCGGGCAGCTCGGCGTTGTGATCAATCTGCTCGACCAGAAGCTGAACAACCTG
AATCCAGATTTCCTGTCGTACACGCTGTCGAAGGCCGCGCTGCAGACTGCCACGGTGCAGCTTGCGCAGGCACTGGCGCC
GCGTCTGCGGGTGGTGGGCGTCGCGCCCGGCATCACGCTGGTGTCCGGCGACCAGTCCGACCCGGGCTTTGTCCGCGCCC
ATGGGATGACGCCGCTGGGCAAGTCGTCCACGCCGGAAGACATCGCGCAGGCCGTGGCCTACCTGGCCGTGGCGCGCGCC
GTTACCGGCACCACGTTGTACGTCGACGGAGGCCAGCACCTGATGCCGCTGGCCCGCGACGTGATGTTCCTTACTGAATA
A

Upstream 100 bases:

>100_bases
GGAAAGGGCCGCGGAGGCGTCCAGCGCATCTGCGGAGGGAAGGGGTAGACTAGCGACTTTCTGCATACCGCGAATTGTAG
CAATGCCAGATTCTCAGACT

Downstream 100 bases:

>100_bases
GCTGTTTCTGATTTGCCAGCCATGACCTTTGCCGCCCTTTCCCATCCCAGCCTGCAAAACTGCCGTCGCATGTTCCTGCG
CAACTATGAAGTGCAGATCA

Product: short chain dehydrogenase

Products: NA

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGTSQASSDKSRGVALVTGAARRLGRAIALELAAQGWDVAVHCNRSRDEADALATQIRGLGRRAAVLQADLSDEAATARL
IPACIDALGVPACLVNNASLFQYDVATSFSYGSLDTHMRTNVAAPLLLAREMHKALGADGNETGQLGVVINLLDQKLNNL
NPDFLSYTLSKAALQTATVQLAQALAPRLRVVGVAPGITLVSGDQSDPGFVRAHGMTPLGKSSTPEDIAQAVAYLAVARA
VTGTTLYVDGGQHLMPLARDVMFLTE

Sequences:

>Translated_266_residues
MGTSQASSDKSRGVALVTGAARRLGRAIALELAAQGWDVAVHCNRSRDEADALATQIRGLGRRAAVLQADLSDEAATARL
IPACIDALGVPACLVNNASLFQYDVATSFSYGSLDTHMRTNVAAPLLLAREMHKALGADGNETGQLGVVINLLDQKLNNL
NPDFLSYTLSKAALQTATVQLAQALAPRLRVVGVAPGITLVSGDQSDPGFVRAHGMTPLGKSSTPEDIAQAVAYLAVARA
VTGTTLYVDGGQHLMPLARDVMFLTE
>Mature_265_residues
GTSQASSDKSRGVALVTGAARRLGRAIALELAAQGWDVAVHCNRSRDEADALATQIRGLGRRAAVLQADLSDEAATARLI
PACIDALGVPACLVNNASLFQYDVATSFSYGSLDTHMRTNVAAPLLLAREMHKALGADGNETGQLGVVINLLDQKLNNLN
PDFLSYTLSKAALQTATVQLAQALAPRLRVVGVAPGITLVSGDQSDPGFVRAHGMTPLGKSSTPEDIAQAVAYLAVARAV
TGTTLYVDGGQHLMPLARDVMFLTE

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=30.078125, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=33.0645161290323, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=29.718875502008, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=28.7449392712551, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787891, Length=241, Percent_Identity=24.896265560166, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=28.4, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=28.8, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=29.811320754717, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=29.5719844357977, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=28.90625, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=27.734375, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27817; Mature: 27686

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTSQASSDKSRGVALVTGAARRLGRAIALELAAQGWDVAVHCNRSRDEADALATQIRGL
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHC
GRRAAVLQADLSDEAATARLIPACIDALGVPACLVNNASLFQYDVATSFSYGSLDTHMRT
CHHHHHEECCCCCHHHHHHHHHHHHHHHCCCHHEECCCHHEEEEHHCCCCCCCCCHHHHH
NVAAPLLLAREMHKALGADGNETGQLGVVINLLDQKLNNLNPDFLSYTLSKAALQTATVQ
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAQALAPRLRVVGVAPGITLVSGDQSDPGFVRAHGMTPLGKSSTPEDIAQAVAYLAVARA
HHHHHCCCEEEEEECCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
VTGTTLYVDGGQHLMPLARDVMFLTE
HCCCEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTSQASSDKSRGVALVTGAARRLGRAIALELAAQGWDVAVHCNRSRDEADALATQIRGL
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHC
GRRAAVLQADLSDEAATARLIPACIDALGVPACLVNNASLFQYDVATSFSYGSLDTHMRT
CHHHHHEECCCCCHHHHHHHHHHHHHHHCCCHHEECCCHHEEEEHHCCCCCCCCCHHHHH
NVAAPLLLAREMHKALGADGNETGQLGVVINLLDQKLNNLNPDFLSYTLSKAALQTATVQ
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAQALAPRLRVVGVAPGITLVSGDQSDPGFVRAHGMTPLGKSSTPEDIAQAVAYLAVARA
HHHHHCCCEEEEEECCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
VTGTTLYVDGGQHLMPLARDVMFLTE
HCCCEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]