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The map label for this gene is cca

Identifier: 94309124

GI number: 94309124

Start: 186333

End: 187577

Strand: Direct

Name: cca

Synonym: Rmet_0179

Alternate gene names: 94309124

Gene position: 186333-187577 (Clockwise)

Preceding gene: 94309123

Following gene: 94309127

Centisome position: 4.74

GC content: 68.19

Gene sequence:

>1245_bases
ATGCAGGTGTACGCAGTGGGCGGCGCCGTCCGCGACCAGTTGCTCGGAAAGCCGAGCCAGGACCGTGACTACGTGGTGGT
GGGCGCCACGCCCGCGGACATGGAGGCCGCCGGCTTCAAGCCGGTGGGCAAGGATTTCCCCGTTTTCCTCCATCCGCACA
CCAAGGCCGAGTACGCGCTGGCGCGGACCGAACGCAAGACCGCGGTCGGCTACAAGGGCTTTGCCTTCTATACCGGCGCC
GACGTGACCCTGGAAGAGGACCTCGTCCGGCGCGACCTGACCATCAACGCGATGGCCCAGGCGGTGGACGAGCATGACAA
CCTCGTCGGCCCGGTGATCGACCCCTATGGCGGCCAGAAGGACCTGGCCGCGCGCAAATTCCGCCACGTGTCCGACGCCT
TCGCCGAGGATCCGGTGCGTATCCTGCGCGTGGCGCGTTTCGCGGCGCGCTTTCACGAATTCACGGTGGCGCCGGAGACC
GTCGCGCTGATGCGCAAGATCGTCGACGCGGGCGAGGTGGATGCGCTGGTGCCCGAGCGCGTCTGGCAGGAACTGTCTCG
CGGGTTGATGGAGGCAAAGCCGTCACGCATGTTCGACGTGCTGCGCGAATGCGGCGCGCTGGCCCACCTGCTGCCGGAAC
TCGATCGCCTATGGGGCGTGCCCCAGCGCCCGGAATATCATCCCGAAGTCGATACCGGCGTGCACGTGATGATGGTGATC
GACTACGCGGCCAGCGTCGGCGCATCGCTGCCGGTGCGCTTCGCCGCGATGGCGCACGACCTCGGCAAGGGCACCACGCC
CGAGGACATGCTGCCCCGGCACATCGGCCACGAGCAACGTGGCGTCAAATTGCTCGAAGCCATCTGCCAGCGCCTGCGCG
TGCCGAACGATTGCCGTGAGCTGGCCCTGGTGGTGGCGCGCGAGCACGGCAATATCCATCGCAGCACGGAGTTCGGCGCG
GCGGCACTGACGCGGCTGGTCGAGCGTTGCGACGCGCTGCGCAAGCCGGAGCGGTTCGCTGAGGCGCTGCTGGCCTGCGA
GGCCGATGCGCGTGGACGCCTGGGTTTTGCGGATAAGGACTACCCGCAGGCGGATCGCCTGCTAGCTGCGCGTGACGCGG
CGGCATCCGTGGACGCCGGCGCGATTGCCAAGGCTTGCGGGGACAAGGTCGATCAGATCAAGGACCGGGTGCACAAGGCC
AGGGTGGCGGCGGTGGCGCAGCGGCTGGGAATGGCGGAGGAATAG

Upstream 100 bases:

>100_bases
GGCGGCGGCGGCACGCGAGGAACGGGACTTTGTCCCGGCCGATGAACCGTATCGCACCGAGCCGGATCGTCCGGACGCGA
TCATCGTTTCGGGTTGATTC

Downstream 100 bases:

>100_bases
GGGGCCAGACATAGGGGGCCAGACATAGGGGGCCGGCCCCCTACAGCAGCTTCCCGATCACCAGCGCCGCGAGCGACAAC
AGGATCGTCGACGCGAATGG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYALARTERKTAVGYKGFAFYTGA
DVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQKDLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPET
VALMRKIVDAGEVDALVPERVWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVI
DYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRELALVVAREHGNIHRSTEFGA
AALTRLVERCDALRKPERFAEALLACEADARGRLGFADKDYPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKA
RVAAVAQRLGMAEE

Sequences:

>Translated_414_residues
MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYALARTERKTAVGYKGFAFYTGA
DVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQKDLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPET
VALMRKIVDAGEVDALVPERVWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVI
DYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRELALVVAREHGNIHRSTEFGA
AALTRLVERCDALRKPERFAEALLACEADARGRLGFADKDYPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKA
RVAAVAQRLGMAEE
>Mature_414_residues
MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYALARTERKTAVGYKGFAFYTGA
DVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQKDLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPET
VALMRKIVDAGEVDALVPERVWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVI
DYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRELALVVAREHGNIHRSTEFGA
AALTRLVERCDALRKPERFAEALLACEADARGRLGFADKDYPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKA
RVAAVAQRLGMAEE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=408, Percent_Identity=53.1862745098039, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87081691, Length=398, Percent_Identity=26.3819095477387, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_RALME (Q1LS11)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582334.1
- ProteinModelPortal:   Q1LS11
- SMR:   Q1LS11
- STRING:   Q1LS11
- GeneID:   4036964
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0179
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   Q1LS11
- ProtClustDB:   PRK10885
- BioCyc:   RMET266264:RMET_0179-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45495; Mature: 45495

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 143-143 BINDING 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYAL
CEEEEECHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAVGYKGFAFYTGADVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCH
DLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPETVALMRKIVDAGEVDALVPER
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
DYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRE
HHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
LALVVAREHGNIHRSTEFGAAALTRLVERCDALRKPERFAEALLACEADARGRLGFADKD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
YPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKARVAAVAQRLGMAEE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYAL
CEEEEECHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAVGYKGFAFYTGADVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQK
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCH
DLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPETVALMRKIVDAGEVDALVPER
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHH
DYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRE
HHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
LALVVAREHGNIHRSTEFGAAALTRLVERCDALRKPERFAEALLACEADARGRLGFADKD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
YPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKARVAAVAQRLGMAEE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA