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The map label for this gene is slt [H]

Identifier: 94309121

GI number: 94309121

Start: 182381

End: 184348

Strand: Direct

Name: slt [H]

Synonym: Rmet_0176

Alternate gene names: 94309121

Gene position: 182381-184348 (Clockwise)

Preceding gene: 94309119

Following gene: 94309122

Centisome position: 4.64

GC content: 64.63

Gene sequence:

>1968_bases
ATGCCGAAGGGAGTATATCTGCGCCGTGCCGGGCGCTTCGCCTGGCTTGCGTTCGCAGGTGCGCTGCTGGCTACCCCAGT
CTACGCGCAGAAACGTACGGCGACGCCCCAGAAAGCGGTACAGCAGCAACCGGCCGCCCTGACGATTCCGTCGGACCCGG
ACGATGCCTTCGTCGCGCTGCGCGAGGCCGCCCGCAAGAATGACGCGGACCGCGCCGCGGCCATCTCGGCCACGCTGGTC
GACTATCCGATTCCGTCGTACGTCGAATACTTCCGCATCAAGTCGCAGATGTTCGACTCGAGCGGCCAGCCCCTGATCGA
TACGCCGGATGCCGAGATCAGCGCGTTCCTGCAACGCTACCAGGGCGAGGCGATTGCCGACCGGATGCGCAATGACTGGC
TGCTGGTGCTGGGCAAGAAGCGCGACTGGGCCAACTTCGATGTCCAGTATCCGCAGTTCGTGCTGAAGGACGATACGCAG
GTGGAGTGCTACGCGCTGCTGTCGCGCGCGCTCAAGGGCCAGAACGTGGCCGCCGATGCGCGTGCGATGCTGGTCGATCC
GCGCTACTACGGCGACGGCTGTGTCGACCTGATCAGCTACATGGCGCAGAGCCAGCAGATCCAGCGCGGCGACGTGGCTT
TCCAGGCACGCCAGGCGCTGGAGCAGAACTACACGACGCTGGCCGGCAAGATCGCCGCAGCGTTGCCCGATGCGCGCGTG
GATGGCGATTCGCTGGCCACCGTGGTCAAGATGGCACGTAATGATCCCTCGCAGGCCGCCGCCTACATGGGCACGATCTC
GGGCAACCTGTCGCGCGACGAGCAGGGTGCGGCCTGGGGCGTGATTGGCCAGTTCGCGGCCAAGAAGCTGATGCCGGAGG
CTGCCGGCTACTACCGTCGCCAAATGGACCTGGGCGGTAACCAGTGGCTGTCCGATGAGTCGCAGGAATGGCGCGTGCGG
GCCGCGTTGCGTCAGGGCGACTGGAAGCAGGTGCGCCAGTCCATCGAATCGATGCGTCCCGAACTGCGTGCCAAGGATCC
GGCATGGACGTACTGGTACGCGCGCGCGCTCAAGGCCGATGGCCGTGGCAGCGATGCCCAGCAGCAGTTCCAGTCGATCG
CCGGCCAGTTCAATTTCTATGGCCAGCTGGCCAGCGAGGAACTGGGCAACCGCATCACGATTCCGACGCGGACCACGGTC
AGCGATGCCGAGGCGATGGCGATGCGCTCGCGCGCCGGCTTCACGCGCGCCAAGAAGTTCTATGACCTGAACCTGCGCTT
CGAAGGCAACCGCGAGTGGAACTGGGAACTGCGCGGCATGAACGATCGTGACCTGCTGGCCGCGGCCGAGTACGCGCGCA
AGATCGAACTGCTCGACCGTACCGTCAACACGTCCGACCGCACCAAGGCCGAGCACGATTTCACGCAGCGCTTCCCGATG
CCGTACCGGGACATCATGCAGCGTGCCACGGACGATGTCGGCCTCGACATGGCATGGGCCTATGGCCTGATCCGTCAGGA
ATCGCGCTTCATCATGAATGCGCGTTCGTCGGCGGGCGCGCATGGCCTGATGCAGGTGATGCCGGCCACGGCCAAGTACG
TCGCGCGCAAGATCGGCATGACCGATTTCTCGCCGTCGATGATGGGAGACCCCAACATCAACATCATGCTCGGCACCAAC
TACATGAGCATGGTGCTGACCGATCTGGACAGCTCCTGGACGCTGGCGTCCGCCGGCTACAACGCCGGTCCGGGACGGCC
CAAGGCGTGGCGTACCAGCCTGTCGCGACCGGTGGAAGGCGCGATCTTTGCAGAGACGATCCCGTTCACCGAGACGCGGA
ACTACGTGAAGAATGTGCTTTCGAACGCCACGTACTATGCTGCTTTAATGAGTGGCCGGCCCCAGTCCCTGAAAGACCGT
CTGGGAATGGTCGCGCCGTCAGCGGTTACGACGACCAGCCTCCCCTGA

Upstream 100 bases:

>100_bases
AATGCTGGACGCGCAAATGAAGATGCGCGCCGGTGGTACGAATCACGAATCCTGCGTGCCTGTCGCGTGACAGGCGCGCA
CCCAACAAGGAATACCAAGA

Downstream 100 bases:

>100_bases
GCCTGACGAGGCACTCTCGCACTCAGACGGAGGCGGTGGGCCCCGTGGCCACGGACGCGCGCATCGATGCCTGCGTAACG
TGTTCGTCACGGAGCCCACC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MPKGVYLRRAGRFAWLAFAGALLATPVYAQKRTATPQKAVQQQPAALTIPSDPDDAFVALREAARKNDADRAAAISATLV
DYPIPSYVEYFRIKSQMFDSSGQPLIDTPDAEISAFLQRYQGEAIADRMRNDWLLVLGKKRDWANFDVQYPQFVLKDDTQ
VECYALLSRALKGQNVAADARAMLVDPRYYGDGCVDLISYMAQSQQIQRGDVAFQARQALEQNYTTLAGKIAAALPDARV
DGDSLATVVKMARNDPSQAAAYMGTISGNLSRDEQGAAWGVIGQFAAKKLMPEAAGYYRRQMDLGGNQWLSDESQEWRVR
AALRQGDWKQVRQSIESMRPELRAKDPAWTYWYARALKADGRGSDAQQQFQSIAGQFNFYGQLASEELGNRITIPTRTTV
SDAEAMAMRSRAGFTRAKKFYDLNLRFEGNREWNWELRGMNDRDLLAAAEYARKIELLDRTVNTSDRTKAEHDFTQRFPM
PYRDIMQRATDDVGLDMAWAYGLIRQESRFIMNARSSAGAHGLMQVMPATAKYVARKIGMTDFSPSMMGDPNINIMLGTN
YMSMVLTDLDSSWTLASAGYNAGPGRPKAWRTSLSRPVEGAIFAETIPFTETRNYVKNVLSNATYYAALMSGRPQSLKDR
LGMVAPSAVTTTSLP

Sequences:

>Translated_655_residues
MPKGVYLRRAGRFAWLAFAGALLATPVYAQKRTATPQKAVQQQPAALTIPSDPDDAFVALREAARKNDADRAAAISATLV
DYPIPSYVEYFRIKSQMFDSSGQPLIDTPDAEISAFLQRYQGEAIADRMRNDWLLVLGKKRDWANFDVQYPQFVLKDDTQ
VECYALLSRALKGQNVAADARAMLVDPRYYGDGCVDLISYMAQSQQIQRGDVAFQARQALEQNYTTLAGKIAAALPDARV
DGDSLATVVKMARNDPSQAAAYMGTISGNLSRDEQGAAWGVIGQFAAKKLMPEAAGYYRRQMDLGGNQWLSDESQEWRVR
AALRQGDWKQVRQSIESMRPELRAKDPAWTYWYARALKADGRGSDAQQQFQSIAGQFNFYGQLASEELGNRITIPTRTTV
SDAEAMAMRSRAGFTRAKKFYDLNLRFEGNREWNWELRGMNDRDLLAAAEYARKIELLDRTVNTSDRTKAEHDFTQRFPM
PYRDIMQRATDDVGLDMAWAYGLIRQESRFIMNARSSAGAHGLMQVMPATAKYVARKIGMTDFSPSMMGDPNINIMLGTN
YMSMVLTDLDSSWTLASAGYNAGPGRPKAWRTSLSRPVEGAIFAETIPFTETRNYVKNVLSNATYYAALMSGRPQSLKDR
LGMVAPSAVTTTSLP
>Mature_654_residues
PKGVYLRRAGRFAWLAFAGALLATPVYAQKRTATPQKAVQQQPAALTIPSDPDDAFVALREAARKNDADRAAAISATLVD
YPIPSYVEYFRIKSQMFDSSGQPLIDTPDAEISAFLQRYQGEAIADRMRNDWLLVLGKKRDWANFDVQYPQFVLKDDTQV
ECYALLSRALKGQNVAADARAMLVDPRYYGDGCVDLISYMAQSQQIQRGDVAFQARQALEQNYTTLAGKIAAALPDARVD
GDSLATVVKMARNDPSQAAAYMGTISGNLSRDEQGAAWGVIGQFAAKKLMPEAAGYYRRQMDLGGNQWLSDESQEWRVRA
ALRQGDWKQVRQSIESMRPELRAKDPAWTYWYARALKADGRGSDAQQQFQSIAGQFNFYGQLASEELGNRITIPTRTTVS
DAEAMAMRSRAGFTRAKKFYDLNLRFEGNREWNWELRGMNDRDLLAAAEYARKIELLDRTVNTSDRTKAEHDFTQRFPMP
YRDIMQRATDDVGLDMAWAYGLIRQESRFIMNARSSAGAHGLMQVMPATAKYVARKIGMTDFSPSMMGDPNINIMLGTNY
MSMVLTDLDSSWTLASAGYNAGPGRPKAWRTSLSRPVEGAIFAETIPFTETRNYVKNVLSNATYYAALMSGRPQSLKDRL
GMVAPSAVTTTSLP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=613, Percent_Identity=23.9804241435563, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73110; Mature: 72978

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGVYLRRAGRFAWLAFAGALLATPVYAQKRTATPQKAVQQQPAALTIPSDPDDAFVAL
CCCCCEEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCEEECCCCCCHHHHHH
REAARKNDADRAAAISATLVDYPIPSYVEYFRIKSQMFDSSGQPLIDTPDAEISAFLQRY
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QGEAIADRMRNDWLLVLGKKRDWANFDVQYPQFVLKDDTQVECYALLSRALKGQNVAADA
CCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCC
RAMLVDPRYYGDGCVDLISYMAQSQQIQRGDVAFQARQALEQNYTTLAGKIAAALPDARV
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGDSLATVVKMARNDPSQAAAYMGTISGNLSRDEQGAAWGVIGQFAAKKLMPEAAGYYRR
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHH
QMDLGGNQWLSDESQEWRVRAALRQGDWKQVRQSIESMRPELRAKDPAWTYWYARALKAD
HHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCC
GRGSDAQQQFQSIAGQFNFYGQLASEELGNRITIPTRTTVSDAEAMAMRSRAGFTRAKKF
CCCCHHHHHHHHHHHCHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHCCHHHHHHE
YDLNLRFEGNREWNWELRGMNDRDLLAAAEYARKIELLDRTVNTSDRTKAEHDFTQRFPM
EEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PYRDIMQRATDDVGLDMAWAYGLIRQESRFIMNARSSAGAHGLMQVMPATAKYVARKIGM
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TDFSPSMMGDPNINIMLGTNYMSMVLTDLDSSWTLASAGYNAGPGRPKAWRTSLSRPVEG
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCC
AIFAETIPFTETRNYVKNVLSNATYYAALMSGRPQSLKDRLGMVAPSAVTTTSLP
CEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKGVYLRRAGRFAWLAFAGALLATPVYAQKRTATPQKAVQQQPAALTIPSDPDDAFVAL
CCCCEEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCEEECCCCCCHHHHHH
REAARKNDADRAAAISATLVDYPIPSYVEYFRIKSQMFDSSGQPLIDTPDAEISAFLQRY
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QGEAIADRMRNDWLLVLGKKRDWANFDVQYPQFVLKDDTQVECYALLSRALKGQNVAADA
CCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCC
RAMLVDPRYYGDGCVDLISYMAQSQQIQRGDVAFQARQALEQNYTTLAGKIAAALPDARV
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGDSLATVVKMARNDPSQAAAYMGTISGNLSRDEQGAAWGVIGQFAAKKLMPEAAGYYRR
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHH
QMDLGGNQWLSDESQEWRVRAALRQGDWKQVRQSIESMRPELRAKDPAWTYWYARALKAD
HHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCC
GRGSDAQQQFQSIAGQFNFYGQLASEELGNRITIPTRTTVSDAEAMAMRSRAGFTRAKKF
CCCCHHHHHHHHHHHCHHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHCCHHHHHHE
YDLNLRFEGNREWNWELRGMNDRDLLAAAEYARKIELLDRTVNTSDRTKAEHDFTQRFPM
EEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PYRDIMQRATDDVGLDMAWAYGLIRQESRFIMNARSSAGAHGLMQVMPATAKYVARKIGM
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
TDFSPSMMGDPNINIMLGTNYMSMVLTDLDSSWTLASAGYNAGPGRPKAWRTSLSRPVEG
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCC
AIFAETIPFTETRNYVKNVLSNATYYAALMSGRPQSLKDRLGMVAPSAVTTTSLP
CEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]