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The map label for this gene is thiD [H]

Identifier: 94309114

GI number: 94309114

Start: 176502

End: 177332

Strand: Reverse

Name: thiD [H]

Synonym: Rmet_0169

Alternate gene names: 94309114

Gene position: 177332-176502 (Counterclockwise)

Preceding gene: 94309120

Following gene: 94309113

Centisome position: 4.51

GC content: 70.76

Gene sequence:

>831_bases
ATGCTCCCCACGCCACCCCGCACCCTGACCATCGCCGGATCCGACTCCGGAGGCGGGGCCGGTATCCAGGCCGATCTGAA
GACGTTCGCGGCACTGGGATGTTTCGGCATGAGCGCGATCACCGCGATCACGGCTCAGAACACGCTCGGCGTGACCGGCG
TCCACGCCATACCTGCCGAGATGGTGGCCGCGCAGATCGACGCCGTGGCCAGCGACATCGGCGTGGACGCGGCCAAGACC
GGCATGCTGGGAACGGCCGCGATCGTCGAGGCCGTGGCCGGGGCGGTGGATCGGCATGGCATCCGCAAGCTTGTGGTGGA
CCCGGTGATGATTTCGACCTCGGGCGCCACGCTGTCCGACGACGCGACCAGCCAGGCGATGGTGAAACTGCTGTTTCCGC
GCGCGATGCTGGTCACGCCGAACCTGCCTGAGGCGTCATACCTGCTGGGCCGCCGCATCACGCGCCGCGCCGAAATGGAG
GCGGCCGCAGCAGACCTGCTTGCGCTCGGTTGCAAGGCGGTGCTGCTCAAGGGCGGCCATCTGGAAGATGACGGGCCCGG
CAGCCTGGGCCTGGACGACCTGCTGATGCTGGCCGACGGCACCGTGCGCGTCTACACCCATCCGCGCGTCGATACGCCGA
ACCTGCACGGCACCGGCTGCACGCTGGCCGCCGCCATCGCGGCGCAGCTCGCCCGGGGCGATGCGCTGCCCGATGCGGTC
GCCGCCGCGCTCGACTTCGTCGCGCAAGCCATCCGCTCGGGCGCCCGGCTGCGCCTTGGCGACGGCAACGGCCCCCTCAA
TCACAGCTTCGCGCCGCGCGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
ACGCTCCTCGGAGTGGCGGTATTGTAGCGTGCCTGTCCGCGCCTTGCGGCGTGAACAGGCACATTTTTCCGGTCTTTCTC
CGTCTCTCCTCACCTTGCAC

Downstream 100 bases:

>100_bases
TCGCGAGCATCGCGAAAACCATGCAGACACTGACCAACATCGACCTGCGCGAAGACCGCGCCGCCAACTGGTACGTCAAG
GATGAAACGGTGCAGGTGGC

Product: bifunctional hydroxy-methylpyrimidine kinase/hydroxy-phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAEMVAAQIDAVASDIGVDAAKT
GMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSDDATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEME
AAAADLLALGCKAVLLKGGHLEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAV
AAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG

Sequences:

>Translated_276_residues
MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAEMVAAQIDAVASDIGVDAAKT
GMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSDDATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEME
AAAADLLALGCKAVLLKGGHLEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAV
AAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG
>Mature_276_residues
MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAEMVAAQIDAVASDIGVDAAKT
GMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSDDATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEME
AAAADLLALGCKAVLLKGGHLEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAV
AAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=46.5909090909091, Blast_Score=211, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=36.2637362637363, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=39.051094890511, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324517, Length=274, Percent_Identity=37.2262773722628, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27872; Mature: 27872

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAE
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MVAAQIDAVASDIGVDAAKTGMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSD
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
DATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEMEAAAADLLALGCKAVLLKGGH
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
LEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAV
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
AAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAE
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MVAAQIDAVASDIGVDAAKTGMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSD
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
DATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEMEAAAADLLALGCKAVLLKGGH
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
LEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAV
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
AAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]