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The map label for this gene is thiO [H]

Identifier: 94309108

GI number: 94309108

Start: 172044

End: 173276

Strand: Direct

Name: thiO [H]

Synonym: Rmet_0163

Alternate gene names: 94309108

Gene position: 172044-173276 (Clockwise)

Preceding gene: 94309107

Following gene: 94309109

Centisome position: 4.38

GC content: 70.07

Gene sequence:

>1233_bases
ATGCCGCCGGCAAACAATCTCATGACAGCAGTACAGTCCTTCGATGTCGCCATCCTCGGTGCCGGCCTGGCCGGGCGGTT
ATCCGCCTGGCAGCTCGTGCGTGCCGGCGCCCGCGTGGCGCTGATCGAGCGTGGCGGACCCGACGGCAGCGGCTCGGCCG
CGCATGTGGCGGCGGCCATGCTGGCGCCGCTGGCGGAATCGGCGATTGCCGAGCGCCGCATCGTCGATCTCGGCATGACC
AGCGTCGATCTCTGGCGTACCTGGATCGGCGAATTGCCAGAGCCCGTGTTCTTCCAGGAGGACGGCACGCTGGTGGTCTG
GCACGCGCGTGACCGCAGCGAGGTGTCGCTGTTCACGGGGCGTATGCGTGCCGTGGCGCCGCCCGATCTGGTGCAGCAAC
GCCTGCGCGCGCTCGACGGCGAAGGCGTAGGCCAGATCGAGCCGGCCTTGGCGGGGCGCTTTCCGCAAGGCCTGCTGCTG
CAGGGTGAAGGGCAGCTCGACAACCGAGGCGCGATGCACGCGCTGCTGAACTGCGCCGTGACCGAGGGCTTGCACTGCGT
CTGGGAGGCCGGTGACATCGATGCCGACGCCTTGCCCGCGCTGGGTATTCGCGCTGGCGTGGTGCTCGACTGCCGCGGCC
TGGGCGCGCGCGCCGCATGGACCAATGCGTCCGCGGGCGGCGAGACCGCGCTGCCGGGCCTGCGCGGCCTGCGCGGCGAA
GTTGTGCGCGTCCACGCGCCGGATGTGAAGCTGCATCGCCCGGTGCGACTGCTGCATCCGCGCTACCCGATCTATATCGC
TCCGAAGCCGAACGATCTCTACGTGATCGGCGCCACGGAACTCGAAAGCGAGGACGAATCGCCAATGAGCGTGCGTTCCG
CGCTCGAACTGCTCTCGGCCGCGCATTCGCTGCATCCAGCGTTCGGCGAGGCGCGCGTGCTCGAACTCAATGTCCAGCGG
CGGCCAACCCGCCCCGACCATCTGCCGGCGATTCGCGTCGACCAGCAGGCACGCGTCGTGCGAGTCAACGGACTCTATCG
ACATGGCTGGCTGATCGCGCCGGCCGTGACGGAGGCTGCGTGTGCCGTCGTGCGCGCGATGCTCGGCGGTGACCCCGCCG
CGCACGCGGTGCCAGCCGCGTTGCGCTGGCCCGGCATGATCGAAGCCGTGCAGGAGCGTGGCACCGCGATGCTATCGGCT
AGGGCCGGGGCCGGCAGCGGCCTGCTGCACTAA

Upstream 100 bases:

>100_bases
GTATCCGCAGTACCCGTAGCACCTGAGTCCCGCGCGCGGCGCCCCCACGCGGCGCGGCGGTATTCACCCAACCGGAGGCC
GGACGGCACATACCTTTCTC

Downstream 100 bases:

>100_bases
TCAGACAGACACCATGGAAATCCTGCTCAATGGCCGACCGACCGAGTTGCCCGAAGCCGCCACGCTGGCGGACGCGGTGA
CGGCGGCGGCCATCGCGCCG

Product: thiamine biosynthesis oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAMLAPLAESAIAERRIVDLGMT
SVDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTGRMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLL
QGEGQLDNRGAMHALLNCAVTEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGE
VVRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSAAHSLHPAFGEARVLELNVQR
RPTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAACAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSA
RAGAGSGLLH

Sequences:

>Translated_410_residues
MPPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAMLAPLAESAIAERRIVDLGMT
SVDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTGRMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLL
QGEGQLDNRGAMHALLNCAVTEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGE
VVRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSAAHSLHPAFGEARVLELNVQR
RPTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAACAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSA
RAGAGSGLLH
>Mature_409_residues
PPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAMLAPLAESAIAERRIVDLGMTS
VDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTGRMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLLQ
GEGQLDNRGAMHALLNCAVTEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGEV
VRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSAAHSLHPAFGEARVLELNVQRR
PTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAACAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSAR
AGAGSGLLH

Specific function: May have amino acid oxidase activity in the biosynthesis of thiazole when cysteine gives its thiol group to be inserted into the thiazole molecule and the rest of the molecule is deaminated to give pyruvic acid and ammonia [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: NA

Molecular weight: Translated: 43523; Mature: 43391

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAM
CCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
LAPLAESAIAERRIVDLGMTSVDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHC
RMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLLQGEGQLDNRGAMHALLNCAV
CHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
TEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGE
HCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCC
VVRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSA
EEEEECCCCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
AHSLHPAFGEARVLELNVQRRPTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAA
HHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCEEEEECCEEECCEEEEHHHHHHH
CAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSARAGAGSGLLH
HHHHHHHHCCCCHHHHCCHHHCCCHHHHHHHHCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
PPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAM
CCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
LAPLAESAIAERRIVDLGMTSVDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHC
RMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLLQGEGQLDNRGAMHALLNCAV
CHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
TEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGE
HCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCC
VVRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSA
EEEEECCCCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
AHSLHPAFGEARVLELNVQRRPTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAA
HHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCEEEEECCEEECCEEEEHHHHHHH
CAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSARAGAGSGLLH
HHHHHHHHCCCCHHHHCCHHHCCCHHHHHHHHCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]