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The map label for this gene is metK [H]

Identifier: 94309101

GI number: 94309101

Start: 160510

End: 161673

Strand: Direct

Name: metK [H]

Synonym: Rmet_0156

Alternate gene names: 94309101

Gene position: 160510-161673 (Clockwise)

Preceding gene: 94309096

Following gene: 94309102

Centisome position: 4.09

GC content: 64.78

Gene sequence:

>1164_bases
GTGTCGAACGATTTCCTCTTTACTTCGGAATCTGTCTCCGAAGGCCATCCCGATAAAGTAGCCGACCAGATTTCGGATGC
TGTCCTGGACGCGATCCTTGCCCAGGACAAGTACGCCCGCGTGGCGGCCGAGACGCTTTGCAATACGGGACTTGTCGTGC
TGGCGGGTGAAATCACCACGACCGCCAACGTCGACTACATCCAGGTCGCGCGCGAAACCATCAAGCGCATCGGTTACGAC
AACACCGAGTACGGCATCGACTACAAGGGCTGCGCCGTGCTGGTGGCCTACGACAAGCAATCGCCCGACATCGCCCAGGG
CGTCGACCGCGCGTCGGACGACTACCTGAACCAGGGCGCTGGCGACCAGGGCCTGATGTTCGGCTATGCCTGCGACGAAA
CGCCGGAACTGATGCCGTTCCCGATCTACTACTCGCACCGCCTGGTGGAGCGCCAGTCGCTGCTGCGCCGTGACGGCCGC
CTGCCCTGGCTGCGTCCCGACGCCAAGTCGCAGGTGACCGTGCGCTACGTGGACGGCCGTCCGCACAGCGTGGATACCGT
GGTCCTGTCGACCCAGCACGCGCCGGACATCTCGCAGGCCCAGATCCGTGAAGCCGTGATCGAAGAGATCATCAAGCCGG
TGCTGCCGGCCGAGATGCTCAAGGAAACCAAGTACCTGGTGAACCCGACCGGCCGTTTCGTGATCGGTGGCCCGCAAGGC
GATTGCGGCCTGACCGGCCGCAAGATCATCGTCGATACCTATGGTGGCGCTTCGCCGCACGGTGGCGGTGCGTTCTCGGG
CAAGGATCCGTCGAAGGTGGACCGTTCGGCCGCCTACGCCGCCCGCTACGTGGCCAAGAACGTCGTGGCCGCCGGCCTGG
CGCGCCAGTGCCAGGTGCAGGTCAGCTACGCGATCGGCGTGGCCCGCCCGATCAACGTGACCGTGTACACCGAAGGCACC
GGCAAGATCTCCGACGAGAAGATCGCCGCGCTGGTTCAGGAGCATTTCGACCTGCGCCCGAAGGGCATCGTCCAGATGCT
GGACCTGCTGCGTCCGATCTACGAGAAGACCGCCGCCTATGGCCACTTCGGCCGCGAGGAGCCGGAGTTCTCGTGGGAAG
CCACTGACAAGGCCGCGATTCTGCGCGCTGCCGCCGGCCTGTAA

Upstream 100 bases:

>100_bases
GCGATGCCGCTCTACCCGAGGGGCTTGAAAATACCGCTAAAGCGTCGCCGCATCGTTTCCCGAGGCTGGCACGCAATAGC
CAACTTGGAGAAACCAGATC

Downstream 100 bases:

>100_bases
GCGCCTGCTGCTGCGTCCGGGCGTTCTGCCGGATCAAGACCCCGCCGGTCCTTCGGGAGCGCGCGGGGTTTTTTGTTGAT
CTTGCTGCACAAGACCTCGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSNDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSLLRRDGR
LPWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDISQAQIREAVIEEIIKPVLPAEMLKETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGT
GKISDEKIAALVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL

Sequences:

>Translated_387_residues
MSNDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSLLRRDGR
LPWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDISQAQIREAVIEEIIKPVLPAEMLKETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGT
GKISDEKIAALVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL
>Mature_386_residues
SNDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYDN
TEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSLLRRDGRL
PWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDISQAQIREAVIEEIIKPVLPAEMLKETKYLVNPTGRFVIGGPQGD
CGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGTG
KISDEKIAALVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=59.4736842105263, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=59.2105263157895, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=68.668407310705, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17538498, Length=378, Percent_Identity=59.5238095238095, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=378, Percent_Identity=58.7301587301587, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=57.2890025575448, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=57.1428571428571, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32565905, Length=378, Percent_Identity=53.1746031746032, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=57.2727272727273, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=54.5189504373178, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=56.4356435643564, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=63.0952380952381, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=84.2105263157895, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=59.0078328981723, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.2592592592593, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=55.3191489361702, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=54.7872340425532, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=54.7872340425532, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=52.2546419098143, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=57.8512396694215, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42148; Mature: 42017

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARETIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYSHRLVERQSLLRRDGRLPWLRPDAKSQVTVRYVDGR
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPDISQAQIREAVIEEIIKPVLPAEMLKETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINVTVYTEGTGKISDEKIAALVQEHFDLRPKGIVQMLDLLRPIYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKAAILRAAAGL
CCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SNDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARETIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYSHRLVERQSLLRRDGRLPWLRPDAKSQVTVRYVDGR
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPDISQAQIREAVIEEIIKPVLPAEMLKETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINVTVYTEGTGKISDEKIAALVQEHFDLRPKGIVQMLDLLRPIYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKAAILRAAAGL
CCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA