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The map label for this gene is pyrE

Identifier: 94309095

GI number: 94309095

Start: 154616

End: 155293

Strand: Direct

Name: pyrE

Synonym: Rmet_0150

Alternate gene names: 94309095

Gene position: 154616-155293 (Clockwise)

Preceding gene: 94309092

Following gene: 94309096

Centisome position: 3.94

GC content: 64.75

Gene sequence:

>678_bases
ATGACGCAGCAGACGACGCCGGACGACCTTAGCCAGTCCTTCATCCGCTTTGCACTGGACGCGGGCGTGCTGTCCTTCGG
CGAGTTCGTGACCAAGGCTGGCCGCAAGTCGCCGTACTTCTTCAACGCCGGTTTGTTCAACCAGGGCGCGATGCTGGGCC
AGGTGGCGCAATTCTACGCGAAGACCCTGCTGGAATCGGGCGTGCAGTTCGACGTGCTGTTCGGGCCGGCCTACAAGGGC
ATCACCCTGGCCTCCGCCACGGCGGTGGCGCTGGCCGGTATGGGGCGTGACGTCGGCTTCTCCTACAACCGCAAGGAAGC
GAAGGACCATGGCGAAGGCGGCACACTGGTCGGCGCCAAGCTTCAGGGCAAGGTCGTGATCATTGACGACGTGATCTCCG
CCGGTACCTCGGTGCGCGAGTCGATGCAACTGATTCGCGCCGCGGGCGCCGAGCCTGCCGCCGTGCTGATCGCGCTGGAC
CGCATGGAAAAGAGCGGCACGGCCGACAACATCGGCACGCATTCGGCGGTGCAGGATGTGCAGCGCGAGTACGGCATTCC
GGTGATCGCGATCGCCACGCTCAAGGACCTGCTGGCGTATCTGGATGCGTCGAAAGACCCGTCGCTCGGGAACTCCCGGG
AGGCTGTGGCAGCCTATCGTCAGAAGTACGGCGTCTGA

Upstream 100 bases:

>100_bases
TCCACTCGAAGAAGCCTTTCTTGGAGGCGGAGCGGATGCCGTTGAGGTTGGCGCTGATAATCCGTAACATCTCGGGAAAT
TTTGAATTCAGAGAGAAAAG

Downstream 100 bases:

>100_bases
CAACGAACACGGCCCCGGATGGGGCCGTTTCTTTTTGGGAGCATGGCGTGGCGGTCAAGCGGCGAAACAAGGAAGCGATC
CAAAGGGAAATCGGCGAACA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLESGVQFDVLFGPAYKG
ITLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALD
RMEKSGTADNIGTHSAVQDVQREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV

Sequences:

>Translated_225_residues
MTQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLESGVQFDVLFGPAYKG
ITLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALD
RMEKSGTADNIGTHSAVQDVQREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV
>Mature_224_residues
TQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLESGVQFDVLFGPAYKGI
TLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDR
MEKSGTADNIGTHSAVQDVQREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790073, Length=215, Percent_Identity=56.2790697674419, Blast_Score=240, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6323530, Length=222, Percent_Identity=43.2432432432432, Blast_Score=189, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=226, Percent_Identity=39.8230088495575, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_RALME (Q1LS40)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582305.1
- ProteinModelPortal:   Q1LS40
- SMR:   Q1LS40
- STRING:   Q1LS40
- GeneID:   4036934
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0150
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q1LS40
- ProtClustDB:   PRK00455
- BioCyc:   RMET266264:RMET_0150-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23821; Mature: 23690

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 32-32 BINDING 104-104 BINDING 105-105 BINDING 108-108 BINDING 110-110 BINDING 133-133 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
KTLLESGVQFDVLFGPAYKGITLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAK
HHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEE
LQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDRMEKSGTADNIGTHSAVQDV
ECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHH
QREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV
HHHHCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
KTLLESGVQFDVLFGPAYKGITLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAK
HHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEE
LQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDRMEKSGTADNIGTHSAVQDV
ECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHH
QREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV
HHHHCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA