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The map label for this gene is liaS [H]

Identifier: 94309037

GI number: 94309037

Start: 99240

End: 100625

Strand: Reverse

Name: liaS [H]

Synonym: Rmet_0092

Alternate gene names: 94309037

Gene position: 100625-99240 (Counterclockwise)

Preceding gene: 94309040

Following gene: 94309031

Centisome position: 2.56

GC content: 65.15

Gene sequence:

>1386_bases
ATGGCCAAGCATTCCCTACTCGTGCGAAACACACTGCTGCTGGTCAGCGGCATCATGCTGACGCTGGCCGTGCTGATCGC
ATCCGAGACCGGCAACCAGCGCCTGCGCGAGGGATACATCGAGGCAATCCGTTCGCAGCAGCTTCAGAACGACCTGGGCG
ACCTGATCGCCGAACTCGTCAATGCAGAGGGCGGCCAGCGCGGCTTCCTGCTGACCGGGAAGGACAGCTACCTCGACCCG
TACTACAAGGCGCTGCCGCGCATCAACGAACTGATGTCGCGCATCCGCCAGCACTACGCGAACGATCCGGAAGGCCTGCG
CCAATTCGGCGAGGCGTCCCAATTCGTGACGCGCAAACTCAACGAGATGGCACTGACGCTGGTCTACGGCAAGCGCGACT
TCGACGTGGCGCTCGACATGGTCCGCACCGACTTCGGCAAGCAGACCATGGAGAACGCCCGGCGCAGTCTCGAGCATCTG
CAGGTGCGCGAGTCGGGCACGGTCACGCACAATCTCGAAGCGGCCGAGCATGACCTGCAACTGTCGCGCTATGGCATTGG
GCTGCTCACGGCCATCAACATCGTATTGATGGTGACGCTCGGCATGGGCCACGCGCGCCGCATGGCAATGGCCGATGCCA
CCCGGGCCGAACTGGAAGAGGAAAGCGCGCGGCTCGACCGCAAGGTGCGCGCCCGCACGCGCCAGCTGTCCGCCCTGGCC
GCTCACCTGCAACGCGTGACCGAAGACGAAAAGACGCGCCTGGCGCGCGAGCTGCACGACGAACTCGGCGCCATCCTCAC
CGCCATCAAGCTCGATCTGCACTGGGTGCGGATGCGCGTGCAAGACACGCATCCGGAGGCGCGCGACAAGCTGACGCGCG
TGATGCAGCACGCGGACCAGGCCATCCAGATCAAGCGACAACTGATCGAGGACCTGCGTCCGACCGTGCTGCTGAACCTG
GGCCTGCGCGAGGCCATCGTGCAACTGGTGGAAGATGTCGGCACGCGCAACCAGTGGGATACCGCGCTGGACCTGCCCGA
GACGCTGCCAAGACTGAACGACCCTGCGGCCATCGCGCTGTACCGCATCGTGCAGGAATCGCTAACGAATGCCAGCAAGT
ACGCAGAGGCCAAGCAAGTGTCAGTGACCCTGGCGTGCACGACCGAACATCTGGTGCTGACCGTGCGCGACGACGGCCGT
GGCTTGCCGCCCGATTTCGACGCTGGCAGCATTGCCGGCCATCACGGCCTGCTCGGCATGGAGCAGCGCGTACTGGCGCT
GGGCGGCACGATGGAAGTGGATTCATCGCCGGGACACGGTGTGACGATCGGGATCGAGGTGCCGCTGACCGCGGCGGTGC
TGGCGCAGGAAGAGCCGGCCGAGTGA

Upstream 100 bases:

>100_bases
CGGCCCACGGCTACGATTGACCCCGCCCCTCAATGTGATGCGATGGTCGCCCATCGGGCCGCCGCGCATCTGGAAACGGT
CTTCTAACCGGTAATTCTCA

Downstream 100 bases:

>100_bases
GGGGAAGGCATGGAGGCGCCGATCCAAGGGCCTTGGAATAGGTGACGCAGCAAGGGACGTCAACGTCAGGGCGTCGACAT
TGCGTAGTCCTCGATCACCC

Product: signal transduction histidine kinase containing a CHASE3 sensory domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELVNAEGGQRGFLLTGKDSYLDP
YYKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKLNEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHL
QVRESGTVTHNLEAAEHDLQLSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALA
AHLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQAIQIKRQLIEDLRPTVLLNL
GLREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIALYRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGR
GLPPDFDAGSIAGHHGLLGMEQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE

Sequences:

>Translated_461_residues
MAKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELVNAEGGQRGFLLTGKDSYLDP
YYKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKLNEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHL
QVRESGTVTHNLEAAEHDLQLSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALA
AHLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQAIQIKRQLIEDLRPTVLLNL
GLREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIALYRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGR
GLPPDFDAGSIAGHHGLLGMEQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE
>Mature_460_residues
AKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELVNAEGGQRGFLLTGKDSYLDPY
YKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKLNEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHLQ
VRESGTVTHNLEAAEHDLQLSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALAA
HLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQAIQIKRQLIEDLRPTVLLNLG
LREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIALYRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGRG
LPPDFDAGSIAGHHGLLGMEQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=194, Percent_Identity=30.9278350515464, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788812, Length=205, Percent_Identity=28.2926829268293, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082311, Length=226, Percent_Identity=29.646017699115, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51302; Mature: 51170

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAEGGQRGFLLTGKDSYLDPYYKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKL
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHLQVRESGTVTHNLEAAEHDLQ
HHHHEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHH
LSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AIQIKRQLIEDLRPTVLLNLGLREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIAL
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHH
YRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGRGLPPDFDAGSIAGHHGLLGM
HHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
EQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE
HHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHCCCCCCC
>Mature Secondary Structure 
AKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAEGGQRGFLLTGKDSYLDPYYKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKL
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHLQVRESGTVTHNLEAAEHDLQ
HHHHEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHH
LSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AIQIKRQLIEDLRPTVLLNLGLREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIAL
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHH
YRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGRGLPPDFDAGSIAGHHGLLGM
HHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
EQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE
HHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]