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The map label for this gene is gcvA [H]

Identifier: 94309003

GI number: 94309003

Start: 66623

End: 67516

Strand: Reverse

Name: gcvA [H]

Synonym: Rmet_0058

Alternate gene names: 94309003

Gene position: 67516-66623 (Counterclockwise)

Preceding gene: 94309013

Following gene: 335055477

Centisome position: 1.72

GC content: 66.89

Gene sequence:

>894_bases
TTGCCCGCGCTGGCCGCGCTGCGCACCTTCGAAGCCGCGGCTCGCCACGAGAGCTTTTCGCGGGCCGCCACCGAATTGTT
CGTCACGCACGGCGCGGTCAGCCACCAGATCCGCGCGCTGGAAGAGGAACTGGGGCAGCAACTGTTCGAGCGCAGGGGCA
AGCGCGTGATGCTGACCACCAACGGACGTCTCTATGCCGAGCGCGTGCGTGACGCGCTGTTGCAGATCGCCGACGCCACG
CGCGTGCTGCAGGCCGGCAATCGCGACAAGCGCCTGACCATCAGCACCATGCCGTCCTTTGCCGCGCGCTGGCTGACGCC
CCGCATCGGCAGCTTCATCGAGCAGCATCCGGAACTGGACGTCGAACTGCTGTCGTCGAACTCGCTCGTCGACTTCGCCA
GCGAGGAGGTCGATATCGCGCTGCGCATGGGCACCGGCCACTACCCGGGCCTCTACGTGGAGAGGCTGCTCGACGATGTG
TTCTTCCCCGTCTGCAGCCCGAACTACAACGGTGGCCGCCTGCCCGAGCAGCCGGCCCACATGGCCGGCATGCCGTTGCT
GCGGGGTGAAGGCGATCCGTGGAAACCGTGGTTCGAAGCGGCCGGGCTCGACTGGCCCGAGCCGACCAAGGGCCTGCTGC
TGCAGGATTCGTCGCTGCTGCTGCAGGCTGCGGCCGAAGGACAGGGCGTGGCGCTGATCCGCTCGTCGCTGGCCTACAAC
GACCTGCTGTCCGGCCGGGTGGTGCGATTGTTCGACGTCAGCATTCCATGCCCGTGGCTGCTCCACTTCGTCTGCACGCA
CGCCAGTCTGGAGACACCCAAAGTCCAGGCCTTCCGGCACTGGCTGCTGCCCGAGATGGAGCGATTCCGCAACATCCTCT
CGCAGTGGAACTAG

Upstream 100 bases:

>100_bases
GTGTCTTGTTAGCCAACCTCACATAGCCGAGAGCACCATGGTCTGGAGCGGAGCCTGGAATCCCGACGAACCGCGTAGCT
GGAAGCGCGAGTTTCCGCGC

Downstream 100 bases:

>100_bases
TCTTCGCCTGAATCGTCCGGCTCGGCCAACTCGTCCGATTCGTCCGGCTTGCTGGGTTTCACGCTGGATGCCTTCGGCGA
CTTGCTTGCCCGACCGGCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPALAALRTFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGQQLFERRGKRVMLTTNGRLYAERVRDALLQIADAT
RVLQAGNRDKRLTISTMPSFAARWLTPRIGSFIEQHPELDVELLSSNSLVDFASEEVDIALRMGTGHYPGLYVERLLDDV
FFPVCSPNYNGGRLPEQPAHMAGMPLLRGEGDPWKPWFEAAGLDWPEPTKGLLLQDSSLLLQAAAEGQGVALIRSSLAYN
DLLSGRVVRLFDVSIPCPWLLHFVCTHASLETPKVQAFRHWLLPEMERFRNILSQWN

Sequences:

>Translated_297_residues
MPALAALRTFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGQQLFERRGKRVMLTTNGRLYAERVRDALLQIADAT
RVLQAGNRDKRLTISTMPSFAARWLTPRIGSFIEQHPELDVELLSSNSLVDFASEEVDIALRMGTGHYPGLYVERLLDDV
FFPVCSPNYNGGRLPEQPAHMAGMPLLRGEGDPWKPWFEAAGLDWPEPTKGLLLQDSSLLLQAAAEGQGVALIRSSLAYN
DLLSGRVVRLFDVSIPCPWLLHFVCTHASLETPKVQAFRHWLLPEMERFRNILSQWN
>Mature_296_residues
PALAALRTFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGQQLFERRGKRVMLTTNGRLYAERVRDALLQIADATR
VLQAGNRDKRLTISTMPSFAARWLTPRIGSFIEQHPELDVELLSSNSLVDFASEEVDIALRMGTGHYPGLYVERLLDDVF
FPVCSPNYNGGRLPEQPAHMAGMPLLRGEGDPWKPWFEAAGLDWPEPTKGLLLQDSSLLLQAAAEGQGVALIRSSLAYND
LLSGRVVRLFDVSIPCPWLLHFVCTHASLETPKVQAFRHWLLPEMERFRNILSQWN

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.6122448979592, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.7958477508651, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=28.1456953642384, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=25.7627118644068, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=31.7919075144509, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787128, Length=146, Percent_Identity=33.5616438356164, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787589, Length=264, Percent_Identity=26.1363636363636, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788481, Length=280, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=25.748502994012, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=25.4826254826255, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=237, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=185, Percent_Identity=24.8648648648649, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33383; Mature: 33251

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALAALRTFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGQQLFERRGKRVMLTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NGRLYAERVRDALLQIADATRVLQAGNRDKRLTISTMPSFAARWLTPRIGSFIEQHPELD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCHHHHHHHHHCCCCC
VELLSSNSLVDFASEEVDIALRMGTGHYPGLYVERLLDDVFFPVCSPNYNGGRLPEQPAH
EEEECCCCEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
MAGMPLLRGEGDPWKPWFEAAGLDWPEPTKGLLLQDSSLLLQAAAEGQGVALIRSSLAYN
HCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHH
DLLSGRVVRLFDVSIPCPWLLHFVCTHASLETPKVQAFRHWLLPEMERFRNILSQWN
HHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PALAALRTFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGQQLFERRGKRVMLTT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NGRLYAERVRDALLQIADATRVLQAGNRDKRLTISTMPSFAARWLTPRIGSFIEQHPELD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCHHHHHHHHHCCCCC
VELLSSNSLVDFASEEVDIALRMGTGHYPGLYVERLLDDVFFPVCSPNYNGGRLPEQPAH
EEEECCCCEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
MAGMPLLRGEGDPWKPWFEAAGLDWPEPTKGLLLQDSSLLLQAAAEGQGVALIRSSLAYN
HCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHH
DLLSGRVVRLFDVSIPCPWLLHFVCTHASLETPKVQAFRHWLLPEMERFRNILSQWN
HHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]