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The map label for this gene is gatA1 [H]

Identifier: 94308994

GI number: 94308994

Start: 55671

End: 57170

Strand: Reverse

Name: gatA1 [H]

Synonym: Rmet_0049

Alternate gene names: 94308994

Gene position: 57170-55671 (Counterclockwise)

Preceding gene: 94308995

Following gene: 94308993

Centisome position: 1.46

GC content: 68.4

Gene sequence:

>1500_bases
ATGCCCTTTTCAGCTGATTCCGTGACTTCCCTGCGCCAGATCGCCGATGCCCTGGCGGCGCGCTCCGTTTCCGCCGAGGA
ACTCGCCCGCGAGTACCTGGCCCGCATCGAGGCCGGCCGCGCCCTCAATGCGTTCGTTGCCGTCGACCCCGAACTGACCC
TGGCCCAGGCCCGCGCCGCCGACGAGCGCCGCGCCCAGGGCCAGGCCACGCCGCTGACCGGCGTGCCCATCGCCCACAAG
GACGTCTTCGTGACGCGCGGCTGGAAGTCCACCGCCGGCTCGAAGATGCTGGCCAACTACGAAAGCCCGTTCGACGCCAC
CGTGGTCGAACGCCTGGCCGCCGCCGGCATGGTCACGCTGGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGA
ACGAGAACTCGTTCTTCGGCCCGGTGAGCAACCCGTGGGACACCAGCCGCGTGCCGGGTGGCTCCTCGGGCGGTTCCGCC
GCCGCCGTGGCCGCCGGCCTTGCACCTGCGGCCACCGGCACGGACACCGGTGGCTCGATTCGCCAGCCGGCCTCGTTCTC
GGGCATCACCGGCATCAAGCCGACGTATGGCCGCGTGTCGCGGTACGGCATGATTGCGTTCGCGTCGTCGCTGGACCAGG
GCGGCCCGATGGCCCATTCAGCAGAGGACTGCGCGCTGCTGCTTAATGGCATGGCCGGTTTCGACCCGAAGGATTCGACC
AGCCTGACCCCGGAACTGGGCGGCGTCACCGAGGACTTCACGCGCCTGCTGGGCCAGCCCCGCGCCGGCGCCACCGCCAG
CCAGCCGCTGGCCGGCCTGCGCATCGGCCTGCCGCGCGAGTATTTCGGCAAAGGCCTCTCGGCCGACGTCGAACAGGCCT
TGCGCGCCGCGCTGGCCGAGTATGAGAAGCTGGGCGCGACGCTGGTCGACGTGACCCTGCCGAAGACCGAACTGTCGATC
CCCGTCTACTACATCATCGCGCCGGCCGAGGCATCGTCGAACCTGTCCCGCTTTGATGGCGTGCGCTACGGCCATCGCGC
CGCGCAGTATGGCGACCTGCTCGACATGTACAAGAAGAGCCGCGCCGAAGGCTTCGGCCCCGAGGTCAAGCGCCGGATCA
TGGTGGGCACGTACGTGCTGTCGCACGGCTACTACGACGCCTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCC
GACGACTTCCAGCGCGCGTTCACCCAGTGCGACGTGATCATGGGTCCGGTGGCGCCCACGGTGGCATGGAAGCTCGGCGA
GAAGACTGCCGATCCGGTCCAGATGTACCTGGCGGACATCTTCACGCTGTCCACCAGCCTGGCGGGCCTGCCCGGCATGA
GCGTGCCGTGCGGCTTTGGCGAAGGCAACATGCCTGTTGGCCTGCAGCTTATCGGCAACTATTTCGACGAAGCGCAATTG
TTGCAGACGGCCCATGCGTTCCAGCAGGCCACCGACTGGCACCTGCGCCGCCCGGCCTGA

Upstream 100 bases:

>100_bases
GCCAGCTACCGAAAACGGCCTCTACCTGGTGCCGAAGGTCATCGAGTGACAACGCGGTCTCCGATACCACGTTGCCCACC
ACCCAGACGCCATCATCGAC

Downstream 100 bases:

>100_bases
CAACGCCATGCAAACACGCCCCCTGCCCATCGTCAGCATGCGCGCCGCTGCGGCCATGGCCCTGCTGGCGACGCTGGCCG
GCTGCATTCCGCTGCCGTTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAADERRAQGQATPLTGVPIAHK
DVFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSA
AAVAAGLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDST
SLTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAEYEKLGATLVDVTLPKTELSI
PVYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKSRAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIA
DDFQRAFTQCDVIMGPVAPTVAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQL
LQTAHAFQQATDWHLRRPA

Sequences:

>Translated_499_residues
MPFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAADERRAQGQATPLTGVPIAHK
DVFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSA
AAVAAGLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDST
SLTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAEYEKLGATLVDVTLPKTELSI
PVYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKSRAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIA
DDFQRAFTQCDVIMGPVAPTVAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQL
LQTAHAFQQATDWHLRRPA
>Mature_498_residues
PFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAADERRAQGQATPLTGVPIAHKD
VFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSAA
AVAAGLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDSTS
LTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAEYEKLGATLVDVTLPKTELSIP
VYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKSRAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIAD
DFQRAFTQCDVIMGPVAPTVAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQLL
QTAHAFQQATDWHLRRPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=36.4173228346457, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI195972892, Length=504, Percent_Identity=25.9920634920635, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI166795287, Length=215, Percent_Identity=35.3488372093023, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=38.6813186813187, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=240, Percent_Identity=32.0833333333333, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=33.8541666666667, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=32.1608040201005, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=165, Percent_Identity=37.5757575757576, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=166, Percent_Identity=34.9397590361446, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=166, Percent_Identity=34.9397590361446, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=221, Percent_Identity=28.9592760180996, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=476, Percent_Identity=34.0336134453782, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=460, Percent_Identity=26.9565217391304, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=198, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=40.080971659919, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=26.171875, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=26.171875, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=26.171875, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=26.171875, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=26.0521042084168, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45550774, Length=237, Percent_Identity=33.7552742616034, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648435, Length=237, Percent_Identity=33.7552742616034, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648437, Length=237, Percent_Identity=33.7552742616034, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=25.6214149139579, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=24.8015873015873, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648441, Length=174, Percent_Identity=36.7816091954023, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648439, Length=174, Percent_Identity=36.7816091954023, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53022; Mature: 52891

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAA
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
DERRAQGQATPLTGVPIAHKDVFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTL
HHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEE
GKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSAAAVAAGLAPAATGTDTGGSI
CCCCHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
SLTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHH
YEKLGATLVDVTLPKTELSIPVYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKS
HHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIADDFQRAFTQCDVIMGPVAPT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQTAHAFQQATDWHLRRPA
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
DERRAQGQATPLTGVPIAHKDVFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTL
HHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEE
GKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSAAAVAAGLAPAATGTDTGGSI
CCCCHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
SLTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHH
YEKLGATLVDVTLPKTELSIPVYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKS
HHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIADDFQRAFTQCDVIMGPVAPT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQTAHAFQQATDWHLRRPA
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA