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The map label for this gene is fsr [H]

Identifier: 94308968

GI number: 94308968

Start: 27028

End: 28257

Strand: Direct

Name: fsr [H]

Synonym: Rmet_0023

Alternate gene names: 94308968

Gene position: 27028-28257 (Clockwise)

Preceding gene: 94308966

Following gene: 94308970

Centisome position: 0.69

GC content: 64.96

Gene sequence:

>1230_bases
ATGCGCCCCCGCACCATGAGCACAACCATCAATCCGGCCCATCCGGCCGACCGTGAGGAAGGCACACGCTTCCGCGTGCT
GTACGCGATCAGTTTCGCGCACTTCCTCAACGACATGATCCAGTCGCTGATCCTTGCCATCTATCCGATGCTCAAGGGCG
GCTTCAACCTGAGCTTCACGCAGATCGGTCTGCTGACGCTGACGTATCAGGTTACGGCGTCGCTGCTGCAGCCGGTGGTC
GGCCTGTACACGGACAAGCATCCGAAGCCGCATTCGCTCGCCATCGGCATGGGATTCACGCTGTGTGGCCTGTTGCTGCT
GTCGGTGGCCCCGACCTATGGCGTGCTGCTGGTTGCCGCAGCGCTGGTGGGAACCGGTTCGTCGATCTTCCACCCGGAAT
CGTCGCGTGTGGCGCGGCTGGCGTCGGGCGGACAGCATGGGCTGGCTCAGTCGATCTTCCAGGTGGGCGGTAACGGTGGC
AGCGCGATGGGTCCGCTGCTGGCGGCGTGGATCGTTCACTCGCAGGGCAGCGTGGCGTGGTTCTCGCTGGCCGCGCTGCT
GGCCATCACCGTGCTGTGGCAAATCGGCGGCTGGTACGGCCGGCACCTGGCCGAGCGCGCCGCGAAGAAGAAGGGCGCCG
GCGTCGTGGTCAACAAGCTGCCGAAGTCGACAGTGGTGCGCGCCATCGTGGTGCTGCTGCTGCTGATCTTCTCGAAGTAC
TTCTACATGGCCAGCCTGAACACGTACTACACGTTCTACCTGATGGAGAAGTTCGGCCTGGCGCGTCAGACCTCGCAGAT
CTACCTGTTCCTGTTCCTGTTTGCCGTAGCGGCGGGCACGATCATGGGCGGGCCGATTGGTGACCGTATCGGCCGCAAGC
GCGTGATCTGGGCGTCGATCCTGGGCGTGGCGCCGTTCACGCTGATGCTGCCCCATGCCAACCTGTTCTGGACCGCCGTG
CTGTCCGTGGTGATCGGCTTCATCCTGGCCTCGGCCTTCTCGGCCATCCTGGTGTTCGCCCAGGAACTGATCCCGGGCAA
GGTGGGCATGGTGTCCGGGCTGTTCTTCGGGTTTGCCTTCGGCATGGGCGGTATTGGCGCCGCCGTGCTCGGCCGGCTGG
CCGATGCGCACGGCATCGAGACCGTGTACCAGCTTTGCGCGTTCCTGCCGCTGCTGGGCCTGCTCGCGGTATTCCTGCCG
GACCTGCGCGAGAGGGCGAAGGCGGCTTGA

Upstream 100 bases:

>100_bases
CATTCTCGCAGGACAGGCGGCAGTTTGCCAATTAGCATCGTCACACTTCCCGGCCGCGCGCTGTTCGGGCGCCCGGGCTT
TTGCCAAGCATGACTGGCCA

Downstream 100 bases:

>100_bases
ACGGCTGATGGTTTTAACCCCCTCGCGCCAGAGCGGGAGGGGGCGTCACTTCCCTCCCGCGATCACTTCCCCGTCTTGTC
CTGCGAGACATCCATGATCA

Product: major facilitator superfamily transporter

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFTQIGLLTLTYQVTASLLQPVV
GLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAAALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGG
SAMGPLLAAWIVHSQGSVAWFSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKY
FYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASILGVAPFTLMLPHANLFWTAV
LSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLP
DLRERAKAA

Sequences:

>Translated_409_residues
MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFTQIGLLTLTYQVTASLLQPVV
GLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAAALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGG
SAMGPLLAAWIVHSQGSVAWFSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKY
FYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASILGVAPFTLMLPHANLFWTAV
LSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLP
DLRERAKAA
>Mature_409_residues
MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFTQIGLLTLTYQVTASLLQPVV
GLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAAALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGG
SAMGPLLAAWIVHSQGSVAWFSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKY
FYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASILGVAPFTLMLPHANLFWTAV
LSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLP
DLRERAKAA

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=405, Percent_Identity=65.9259259259259, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43828; Mature: 43828

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFT
CCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QIGLLTLTYQVTASLLQPVVGLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
ALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGGSAMGPLLAAWIVHSQGSVAW
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
FSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHHH
FYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
LGVAPFTLMLPHANLFWTAVLSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLPDLRERAKAA
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFT
CCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QIGLLTLTYQVTASLLQPVVGLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
ALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGGSAMGPLLAAWIVHSQGSVAW
HHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHH
FSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHHH
FYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
LGVAPFTLMLPHANLFWTAVLSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLPDLRERAKAA
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]