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The map label for this gene is gph [C]

Identifier: 93007270

GI number: 93007270

Start: 3020236

End: 3020913

Strand: Direct

Name: gph [C]

Synonym: Pcryo_2446

Alternate gene names: 93007270

Gene position: 3020236-3020913 (Clockwise)

Preceding gene: 93007269

Following gene: 93007272

Centisome position: 98.7

GC content: 44.1

Gene sequence:

>678_bases
ATGGATAAGCAACTTTTAATTTTTGATTTTGATGGTACCCTTATTGATAGCGTGCCAGATTTGGCGGATGCGACCAATGC
GATGCTGACTACCCTTGGCAAAACGCCCTATCCTCTCAATAGCATTCGTAACTGGGTCGGTAATGGCTCAAGAATGCTTG
TTGAGCGCGCCTTGGTTGGTAAGATAGAGGTTTTAGAGGGTGAATTAGCCAAAGAAGCCGTCGACCATGCTGAGCAAGTA
TTTTTCGATGCTTATAAAAATATCAGTGGCAGCAAAACTGTCGCTTACCCCGATGTTGATAGTGGGCTCAGAAAGCTACA
AGCAGCAGGATATGAGCTGGCATTGGTCACCAATAAGCCGATTCGTTTTGTACCAAAAATACTGCAAGCCTTTGGCTGGC
AAGACATCTTTAGCGAAGTGCTTGGCGGTGATAGCTTGTCTAGCAAAAAACCAGACCCCGCGCCGTTATTACATGTCTGT
GAGACGTTGAATGTTACTCCAAAACAAGCGGTCATGATTGGTGATTCAAGAAATGATATCTTAGCGGGACAAAATGCCAA
TATGGACACGCTCGCATTGTCTTATGGTTATAACTATGGGCAAGATATTCGTGAGCTTAATCCTACGGCAGCCTTCGATG
ATTTTAGCGCTTTGGTGGACTATTTATTAAAGAGTTAG

Upstream 100 bases:

>100_bases
ATAGGATAGCAAAATTAAGGCTTACTGGTTTCTTATGCTATGGTAATCAACATTAACCGAATATCAATAAAAAATACAGC
ACTTTTAACGGGTAGAAATA

Downstream 100 bases:

>100_bases
CCTGCAAATATAGTCAGTCTTAAATAAAAGAAGTACAAAAATTATAAGATGCTACTGGTATCAATTTTTCTTGCTTACTG
TGCCTACGCAGACAGAGGCT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDKQLLIFDFDGTLIDSVPDLADATNAMLTTLGKTPYPLNSIRNWVGNGSRMLVERALVGKIEVLEGELAKEAVDHAEQV
FFDAYKNISGSKTVAYPDVDSGLRKLQAAGYELALVTNKPIRFVPKILQAFGWQDIFSEVLGGDSLSSKKPDPAPLLHVC
ETLNVTPKQAVMIGDSRNDILAGQNANMDTLALSYGYNYGQDIRELNPTAAFDDFSALVDYLLKS

Sequences:

>Translated_225_residues
MDKQLLIFDFDGTLIDSVPDLADATNAMLTTLGKTPYPLNSIRNWVGNGSRMLVERALVGKIEVLEGELAKEAVDHAEQV
FFDAYKNISGSKTVAYPDVDSGLRKLQAAGYELALVTNKPIRFVPKILQAFGWQDIFSEVLGGDSLSSKKPDPAPLLHVC
ETLNVTPKQAVMIGDSRNDILAGQNANMDTLALSYGYNYGQDIRELNPTAAFDDFSALVDYLLKS
>Mature_225_residues
MDKQLLIFDFDGTLIDSVPDLADATNAMLTTLGKTPYPLNSIRNWVGNGSRMLVERALVGKIEVLEGELAKEAVDHAEQV
FFDAYKNISGSKTVAYPDVDSGLRKLQAAGYELALVTNKPIRFVPKILQAFGWQDIFSEVLGGDSLSSKKPDPAPLLHVC
ETLNVTPKQAVMIGDSRNDILAGQNANMDTLALSYGYNYGQDIRELNPTAAFDDFSALVDYLLKS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=228, Percent_Identity=38.1578947368421, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24605; Mature: 24605

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKQLLIFDFDGTLIDSVPDLADATNAMLTTLGKTPYPLNSIRNWVGNGSRMLVERALVG
CCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
KIEVLEGELAKEAVDHAEQVFFDAYKNISGSKTVAYPDVDSGLRKLQAAGYELALVTNKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCC
IRFVPKILQAFGWQDIFSEVLGGDSLSSKKPDPAPLLHVCETLNVTPKQAVMIGDSRNDI
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCE
LAGQNANMDTLALSYGYNYGQDIRELNPTAAFDDFSALVDYLLKS
ECCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKQLLIFDFDGTLIDSVPDLADATNAMLTTLGKTPYPLNSIRNWVGNGSRMLVERALVG
CCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
KIEVLEGELAKEAVDHAEQVFFDAYKNISGSKTVAYPDVDSGLRKLQAAGYELALVTNKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCC
IRFVPKILQAFGWQDIFSEVLGGDSLSSKKPDPAPLLHVCETLNVTPKQAVMIGDSRNDI
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCE
LAGQNANMDTLALSYGYNYGQDIRELNPTAAFDDFSALVDYLLKS
ECCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA