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The map label for this gene is thrS [H]

Identifier: 93007241

GI number: 93007241

Start: 2986729

End: 2988663

Strand: Direct

Name: thrS [H]

Synonym: Pcryo_2417

Alternate gene names: 93007241

Gene position: 2986729-2988663 (Clockwise)

Preceding gene: 93007240

Following gene: 93007242

Centisome position: 97.61

GC content: 43.26

Gene sequence:

>1935_bases
ATGGTAGCGATTACTTTACCCGATGGTAGCGTAAAAAACTTCGAAGGTAATACAACGGTCATGGAAGTGGCGCAAAGTAT
TGGGACAGGGCTTGCTAAAGCGACTGTCGCTGGACGTGTGGATGGTCAGTTGGTAGATGCGCATGACCCTATCGCCCATG
ATGCGAAAGTTGAAATCGTGACACCAAAAGATGATGACGGTGTCGATATCATTCGCCATTCTTGTGCTCACCTATTAGGT
CATGCCGTTAAGCAGTTATATCCTGATGTGAAAATGGTGATTGGTCCCGTTATCGATGATGGTTTTTATTATGACATCTA
TAGCGAAACGCCGTTTACGCCTGAGCATATGGCAGCCATTGAAAAACGTATGATGGAGCTCATTAAGCAAGATTATGACG
TTATCAAAAAAATAACGCCACGTGCTGAAGTCATTAGAATCTTTGAAGAACGTGGTGAAGAATATAAGCTTAAGCTGATT
AATGATATGCCAGGCGAAGAAGCCTTTGGTCTATATCATCATCAAGAATATGTCGATATGTGCCGTGGTCCACACGTGCC
AAATACTCGCTTCTTAAAAGTCTTTAAACTGACCAAAATGTCAGGTGCTTATTGGCGCGGCGATGCTAAGAACGAACAGC
TACAACGTATTTATGGTACAGCATGGGCGGATAAGAAAGATTTAAAAGCCTATATCCAGCGTATTGAAGAAGCCGAAAAA
CGTGACCATCGTAAAATTGGTAAAGCGTTAAATCTATTCCATATGCAAGAGCAAGCGCCGGGTATGGTGTTTTGGCATGC
CAATGGTTGGACGATTTATCAAGTACTTGAGCAATATATGCGTAAAGTGCAGCACGATAATGGTTATGAAGAGATTAAAA
CGCCGCAAATTGTCGATCGTAGCTTGTGGGAGCGTTCTGGACATTGGGGCAACTATGCGACCAATATGTTTACCACTTCG
AGCGAAAATCGTGATTATGCGGTAAAACCGATGAACTGTCCTTGTCACGTGCAAGTCTTTAATCAAGGCTTAAAATCTTA
CCGTGACTTGCCATTACGTATGGCAGAGTTTGGTTCATGCCATCGAAACGAGCCATCAGGTTCGCTCCATGGTTTGATGC
GGGTTCGTGGTTTTACTCAAGATGATGCTCATATCTTCTGTACACAGTCGCAGATTCAACAAGAAGTCGCTGATTTTATT
AAGCTGACCCTTGCTGTTTATGAAGATTTTGGTTTTGATAAGATCATTATGAAACTATCGACTCGTCCTGAAAAACGTGT
CGGTAGCGATGAGTCTTGGGATTTTGCAGAAAAAGCATTAGCCGATGCATTGGATAGTTCCGGTCTTGATTGGGCTTATC
TACCAGGTGAAGGCGCATTCTATGGTCCCAAGATTGAATTTAGTCTAAAAGATTCATTAGGTCGCGTATGGCAGTGCGGT
ACTATTCAGGTTGACCCGAATATGCCTGAGCGTCTTGATGCCGAATTCGTCAATGAGCAAAACGAGCGCGAAGTACCGAT
TATGTTGCACCGTGCCATCTTAGGTTCATTTGAGCGCTTTATCGGTATCTTGATTGAGAACTATGCCGGTTGGATGCCAG
TATGGCTAGCGCCGCAACAAGTAGTAGTCATGAATATCACAGATAAACAGGCAGATGCATGTGAAAATGTGGTCAATGAG
TTGAAAAAAGCAGGCTTACGCGCCATTAGTGACTTGCGTAACGAAAAGATTGGATTTAAGATACGAGAGAAAACATTAGA
ACGTATTCCCTATATGCTAGTATTAGGGGATAAAGAAGTCGAATCAGGCAGCATCAACGTTAGGACTCGCGAAGGCGAAA
ATCTAGGCGTGATGAGTGTCGCTGAATTTATCACATTAGTAGAAGCAGCTGTTGCCGAAAAGGGCCGACAGATCCCAAAA
ATAGATCAGGAGTAA

Upstream 100 bases:

>100_bases
AACATATCATAGTAATTAGATATAATTGACGCAGTCTTACGGTTATATGATTGATGGTTGCAATCATCACTGCCAACGGA
CGCAAAAAGGTGAATAGATT

Downstream 100 bases:

>100_bases
TACCTATTAAACAGTCAAACCGTTTGAACATTAATGAAGATATCAGTGCCAAAGAAGTCCGTCTGGTCAAAGAAGACGGT
GAACAAATGGGCGTGGTCGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MVAITLPDGSVKNFEGNTTVMEVAQSIGTGLAKATVAGRVDGQLVDAHDPIAHDAKVEIVTPKDDDGVDIIRHSCAHLLG
HAVKQLYPDVKMVIGPVIDDGFYYDIYSETPFTPEHMAAIEKRMMELIKQDYDVIKKITPRAEVIRIFEERGEEYKLKLI
NDMPGEEAFGLYHHQEYVDMCRGPHVPNTRFLKVFKLTKMSGAYWRGDAKNEQLQRIYGTAWADKKDLKAYIQRIEEAEK
RDHRKIGKALNLFHMQEQAPGMVFWHANGWTIYQVLEQYMRKVQHDNGYEEIKTPQIVDRSLWERSGHWGNYATNMFTTS
SENRDYAVKPMNCPCHVQVFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTQSQIQQEVADFI
KLTLAVYEDFGFDKIIMKLSTRPEKRVGSDESWDFAEKALADALDSSGLDWAYLPGEGAFYGPKIEFSLKDSLGRVWQCG
TIQVDPNMPERLDAEFVNEQNEREVPIMLHRAILGSFERFIGILIENYAGWMPVWLAPQQVVVMNITDKQADACENVVNE
LKKAGLRAISDLRNEKIGFKIREKTLERIPYMLVLGDKEVESGSINVRTREGENLGVMSVAEFITLVEAAVAEKGRQIPK
IDQE

Sequences:

>Translated_644_residues
MVAITLPDGSVKNFEGNTTVMEVAQSIGTGLAKATVAGRVDGQLVDAHDPIAHDAKVEIVTPKDDDGVDIIRHSCAHLLG
HAVKQLYPDVKMVIGPVIDDGFYYDIYSETPFTPEHMAAIEKRMMELIKQDYDVIKKITPRAEVIRIFEERGEEYKLKLI
NDMPGEEAFGLYHHQEYVDMCRGPHVPNTRFLKVFKLTKMSGAYWRGDAKNEQLQRIYGTAWADKKDLKAYIQRIEEAEK
RDHRKIGKALNLFHMQEQAPGMVFWHANGWTIYQVLEQYMRKVQHDNGYEEIKTPQIVDRSLWERSGHWGNYATNMFTTS
SENRDYAVKPMNCPCHVQVFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTQSQIQQEVADFI
KLTLAVYEDFGFDKIIMKLSTRPEKRVGSDESWDFAEKALADALDSSGLDWAYLPGEGAFYGPKIEFSLKDSLGRVWQCG
TIQVDPNMPERLDAEFVNEQNEREVPIMLHRAILGSFERFIGILIENYAGWMPVWLAPQQVVVMNITDKQADACENVVNE
LKKAGLRAISDLRNEKIGFKIREKTLERIPYMLVLGDKEVESGSINVRTREGENLGVMSVAEFITLVEAAVAEKGRQIPK
IDQE
>Mature_644_residues
MVAITLPDGSVKNFEGNTTVMEVAQSIGTGLAKATVAGRVDGQLVDAHDPIAHDAKVEIVTPKDDDGVDIIRHSCAHLLG
HAVKQLYPDVKMVIGPVIDDGFYYDIYSETPFTPEHMAAIEKRMMELIKQDYDVIKKITPRAEVIRIFEERGEEYKLKLI
NDMPGEEAFGLYHHQEYVDMCRGPHVPNTRFLKVFKLTKMSGAYWRGDAKNEQLQRIYGTAWADKKDLKAYIQRIEEAEK
RDHRKIGKALNLFHMQEQAPGMVFWHANGWTIYQVLEQYMRKVQHDNGYEEIKTPQIVDRSLWERSGHWGNYATNMFTTS
SENRDYAVKPMNCPCHVQVFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTQSQIQQEVADFI
KLTLAVYEDFGFDKIIMKLSTRPEKRVGSDESWDFAEKALADALDSSGLDWAYLPGEGAFYGPKIEFSLKDSLGRVWQCG
TIQVDPNMPERLDAEFVNEQNEREVPIMLHRAILGSFERFIGILIENYAGWMPVWLAPQQVVVMNITDKQADACENVVNE
LKKAGLRAISDLRNEKIGFKIREKTLERIPYMLVLGDKEVESGSINVRTREGENLGVMSVAEFITLVEAAVAEKGRQIPK
IDQE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=640, Percent_Identity=37.96875, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI38570111, Length=628, Percent_Identity=37.8980891719745, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI20070344, Length=651, Percent_Identity=35.6374807987711, Blast_Score=388, Evalue=1e-107,
Organism=Escherichia coli, GI1788013, Length=644, Percent_Identity=61.6459627329193, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=281, Percent_Identity=24.1992882562278, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=630, Percent_Identity=38.4126984126984, Blast_Score=471, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71984192, Length=630, Percent_Identity=38.4126984126984, Blast_Score=471, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322112, Length=630, Percent_Identity=39.6825396825397, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=38.7909319899244, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=39.3650793650794, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=39.3650793650794, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=39.3650793650794, Blast_Score=479, Evalue=1e-135,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73377; Mature: 73377

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAITLPDGSVKNFEGNTTVMEVAQSIGTGLAKATVAGRVDGQLVDAHDPIAHDAKVEIV
CEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
TPKDDDGVDIIRHSCAHLLGHAVKQLYPDVKMVIGPVIDDGFYYDIYSETPFTPEHMAAI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
EKRMMELIKQDYDVIKKITPRAEVIRIFEERGEEYKLKLINDMPGEEAFGLYHHQEYVDM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
CRGPHVPNTRFLKVFKLTKMSGAYWRGDAKNEQLQRIYGTAWADKKDLKAYIQRIEEAEK
HCCCCCCCHHHEEEEEHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDHRKIGKALNLFHMQEQAPGMVFWHANGWTIYQVLEQYMRKVQHDNGYEEIKTPQIVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHCCCCHHHCCCCHHHHH
SLWERSGHWGNYATNMFTTSSENRDYAVKPMNCPCHVQVFNQGLKSYRDLPLRMAEFGSC
HHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGSLHGLMRVRGFTQDDAHIFCTQSQIQQEVADFIKLTLAVYEDFGFDKIIMKLS
CCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
TRPEKRVGSDESWDFAEKALADALDSSGLDWAYLPGEGAFYGPKIEFSLKDSLGRVWQCG
CCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEECCEEEEEEHHCCCCEEECC
TIQVDPNMPERLDAEFVNEQNEREVPIMLHRAILGSFERFIGILIENYAGWMPVWLAPQQ
EEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCE
VVVMNITDKQADACENVVNELKKAGLRAISDLRNEKIGFKIREKTLERIPYMLVLGDKEV
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCEEEEECCCCC
ESGSINVRTREGENLGVMSVAEFITLVEAAVAEKGRQIPKIDQE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVAITLPDGSVKNFEGNTTVMEVAQSIGTGLAKATVAGRVDGQLVDAHDPIAHDAKVEIV
CEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
TPKDDDGVDIIRHSCAHLLGHAVKQLYPDVKMVIGPVIDDGFYYDIYSETPFTPEHMAAI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
EKRMMELIKQDYDVIKKITPRAEVIRIFEERGEEYKLKLINDMPGEEAFGLYHHQEYVDM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
CRGPHVPNTRFLKVFKLTKMSGAYWRGDAKNEQLQRIYGTAWADKKDLKAYIQRIEEAEK
HCCCCCCCHHHEEEEEHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDHRKIGKALNLFHMQEQAPGMVFWHANGWTIYQVLEQYMRKVQHDNGYEEIKTPQIVDR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHCCCCHHHCCCCHHHHH
SLWERSGHWGNYATNMFTTSSENRDYAVKPMNCPCHVQVFNQGLKSYRDLPLRMAEFGSC
HHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGSLHGLMRVRGFTQDDAHIFCTQSQIQQEVADFIKLTLAVYEDFGFDKIIMKLS
CCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
TRPEKRVGSDESWDFAEKALADALDSSGLDWAYLPGEGAFYGPKIEFSLKDSLGRVWQCG
CCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEECCEEEEEEHHCCCCEEECC
TIQVDPNMPERLDAEFVNEQNEREVPIMLHRAILGSFERFIGILIENYAGWMPVWLAPQQ
EEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCE
VVVMNITDKQADACENVVNELKKAGLRAISDLRNEKIGFKIREKTLERIPYMLVLGDKEV
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCEEEEECCCCC
ESGSINVRTREGENLGVMSVAEFITLVEAAVAEKGRQIPKIDQE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA