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The map label for this gene is gltX [H]

Identifier: 93007205

GI number: 93007205

Start: 2928256

End: 2929785

Strand: Direct

Name: gltX [H]

Synonym: Pcryo_2381

Alternate gene names: 93007205

Gene position: 2928256-2929785 (Clockwise)

Preceding gene: 93007197

Following gene: 93007206

Centisome position: 95.7

GC content: 47.84

Gene sequence:

>1530_bases
ATGATGCAACCATCTACTTCTACTAATAGCATACGCCCTGTTCGTACCCGTATTGCCCCTTCGCCTACTGGCTTCCCGCA
CGTTGGCACTGCTTATATCGCACTGTTTAATTTAGCCTTTGCCAAGGCTCATGGCGGCGAATTTATTTTGCGTATTGAAG
ACACCGACCAAACGCGCTCGACAGAGCAATCTGAAAAAATGATTTTGGATGCACTACGTTGGGTCGGTCTCGACTGGGCG
GAAGGTCCAGATATCGGCGGTCCGCACGCGCCTTATCGTCAAAGCGAGCGTAGTGATATTTACAAAAAGCACGCCGAGCA
GCTGATAGAAAACGATCATGCGTTTCGCTGCTTTTGTAGCAGTGAAGAGCTTGATGCCATGCGTGCTGAGCAAATGGCCA
ATGGCGAGACCCCACGTTATGATGGTCGCTGTGCGCATTTAGCCGCAGAAAAAACCGAGCAATTGGTCGCTGAGGGCAAA
CCGCACGTCATCCGTATGCGCGTGCCTACCGAAGGCGTCTGTCAAGTGCAAGACATGCTACGCGGTACGGTTGAGATTCC
TTGGACCCAAGTCGACATGCAAGTACTGCTAAAAACAGACGGCATGCCAACATACCATTTAGCCAATGTCGTCGACGACC
ATTTGATGGATATCAGTCATGTGATGCGTGGTGAAGAATGGTTGAATTCTGCGCCTAAGCATCAGTTGCTTTATGAGTAT
TTTGGTTGGGAGATGCCTGTACTTTGCCACATGCCATTACTGCGTAACCCAGATAAATCAAAACTGTCTAAGCGTAAAAA
TCCAACGTCTATCACCTATTATCGTGATGCGGGCGTACTCCCTGAAGCGTTGCTAAACTATCTCGGTCGTATGGGCTACT
CAATGCCTGACGAAGCGGAGCAGTTTACCTTAGAGGAAATGATTGCCAGCTTTGATATTCAGCGTGTGTCGCTTGGCGGC
CCTATTTTCGATATCGAGAAACTCAACTGGCTCAATTCTGAATGGCTACGTGCACTCACGCCAGAAGAGCTAAAAAATAA
AATCCTTGAATGGGCAAGCAACAGTGACAAGCTAACCGCTATCGCAGCGGCAATTCAACCACGTATCGAATTGTTATCTG
ATGCGGTCAATTGGAGTGGCTTCTATTTTCAAAACTTACCTGATATCAACGCTGAGAGCTTTACTCATAAGTCGCTCACA
CCTGAGCAAATCATGGATATGCTACAGCTGTCATTATGGCAGCTAGAAGTCTTGCCTACTTGGTCAGAAGAAAACATCTA
CGCCACGCTAAAAGGCCTTGCTGCACATCTTGATATTAAAATGCGTGACTTTATGGCGCCGTTCTTTATCGCTATCGCTG
GTAGCACCTCATCGACGCCTGTCATGAACTCTATGGCGATTATTGGTGCTGATATGACGCTGACACGCTTGCGTCATGCG
GTTGACGTGCTGGGTGGCCTCGGTAAAAAGAAACTCAAAAAACTAGAGAAACAAGCAGCAGAATTGCCAGACTTTTTGGC
AGCAGAATAG

Upstream 100 bases:

>100_bases
CTATTTTTCAACGTTGACTGCCATTCTTTCGTCCATATTTTCACTTTTCACTACTAGTTTTGACCGTCCCATTCATTAAC
AACAACATTCTGAGATTTTT

Downstream 100 bases:

>100_bases
TTTCTTAGTTGTTTGTTAATAACATTGTTGATTCATAACATTATTAGTGAATAAAAAAGAGGGGTCAGACTAATACTGAC
CCCTCTTTACGTGAATCTCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MMQPSTSTNSIRPVRTRIAPSPTGFPHVGTAYIALFNLAFAKAHGGEFILRIEDTDQTRSTEQSEKMILDALRWVGLDWA
EGPDIGGPHAPYRQSERSDIYKKHAEQLIENDHAFRCFCSSEELDAMRAEQMANGETPRYDGRCAHLAAEKTEQLVAEGK
PHVIRMRVPTEGVCQVQDMLRGTVEIPWTQVDMQVLLKTDGMPTYHLANVVDDHLMDISHVMRGEEWLNSAPKHQLLYEY
FGWEMPVLCHMPLLRNPDKSKLSKRKNPTSITYYRDAGVLPEALLNYLGRMGYSMPDEAEQFTLEEMIASFDIQRVSLGG
PIFDIEKLNWLNSEWLRALTPEELKNKILEWASNSDKLTAIAAAIQPRIELLSDAVNWSGFYFQNLPDINAESFTHKSLT
PEQIMDMLQLSLWQLEVLPTWSEENIYATLKGLAAHLDIKMRDFMAPFFIAIAGSTSSTPVMNSMAIIGADMTLTRLRHA
VDVLGGLGKKKLKKLEKQAAELPDFLAAE

Sequences:

>Translated_509_residues
MMQPSTSTNSIRPVRTRIAPSPTGFPHVGTAYIALFNLAFAKAHGGEFILRIEDTDQTRSTEQSEKMILDALRWVGLDWA
EGPDIGGPHAPYRQSERSDIYKKHAEQLIENDHAFRCFCSSEELDAMRAEQMANGETPRYDGRCAHLAAEKTEQLVAEGK
PHVIRMRVPTEGVCQVQDMLRGTVEIPWTQVDMQVLLKTDGMPTYHLANVVDDHLMDISHVMRGEEWLNSAPKHQLLYEY
FGWEMPVLCHMPLLRNPDKSKLSKRKNPTSITYYRDAGVLPEALLNYLGRMGYSMPDEAEQFTLEEMIASFDIQRVSLGG
PIFDIEKLNWLNSEWLRALTPEELKNKILEWASNSDKLTAIAAAIQPRIELLSDAVNWSGFYFQNLPDINAESFTHKSLT
PEQIMDMLQLSLWQLEVLPTWSEENIYATLKGLAAHLDIKMRDFMAPFFIAIAGSTSSTPVMNSMAIIGADMTLTRLRHA
VDVLGGLGKKKLKKLEKQAAELPDFLAAE
>Mature_509_residues
MMQPSTSTNSIRPVRTRIAPSPTGFPHVGTAYIALFNLAFAKAHGGEFILRIEDTDQTRSTEQSEKMILDALRWVGLDWA
EGPDIGGPHAPYRQSERSDIYKKHAEQLIENDHAFRCFCSSEELDAMRAEQMANGETPRYDGRCAHLAAEKTEQLVAEGK
PHVIRMRVPTEGVCQVQDMLRGTVEIPWTQVDMQVLLKTDGMPTYHLANVVDDHLMDISHVMRGEEWLNSAPKHQLLYEY
FGWEMPVLCHMPLLRNPDKSKLSKRKNPTSITYYRDAGVLPEALLNYLGRMGYSMPDEAEQFTLEEMIASFDIQRVSLGG
PIFDIEKLNWLNSEWLRALTPEELKNKILEWASNSDKLTAIAAAIQPRIELLSDAVNWSGFYFQNLPDINAESFTHKSLT
PEQIMDMLQLSLWQLEVLPTWSEENIYATLKGLAAHLDIKMRDFMAPFFIAIAGSTSSTPVMNSMAIIGADMTLTRLRHA
VDVLGGLGKKKLKKLEKQAAELPDFLAAE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=34.4, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=36.0416666666667, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI226510925, Length=296, Percent_Identity=29.3918918918919, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=34.7280334728033, Blast_Score=271, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6324540, Length=349, Percent_Identity=41.5472779369628, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=309, Percent_Identity=24.9190938511327, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=35.8606557377049, Blast_Score=301, Evalue=6e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57494; Mature: 57494

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPSTSTNSIRPVRTRIAPSPTGFPHVGTAYIALFNLAFAKAHGGEFILRIEDTDQTRS
CCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TEQSEKMILDALRWVGLDWAEGPDIGGPHAPYRQSERSDIYKKHAEQLIENDHAFRCFCS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
SEELDAMRAEQMANGETPRYDGRCAHLAAEKTEQLVAEGKPHVIRMRVPTEGVCQVQDML
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
RGTVEIPWTQVDMQVLLKTDGMPTYHLANVVDDHLMDISHVMRGEEWLNSAPKHQLLYEY
CCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
FGWEMPVLCHMPLLRNPDKSKLSKRKNPTSITYYRDAGVLPEALLNYLGRMGYSMPDEAE
HCCCCCHHHCCHHHCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHH
QFTLEEMIASFDIQRVSLGGPIFDIEKLNWLNSEWLRALTPEELKNKILEWASNSDKLTA
HHHHHHHHHHCCCEEEECCCCEEEHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCHHHH
IAAAIQPRIELLSDAVNWSGFYFQNLPDINAESFTHKSLTPEQIMDMLQLSLWQLEVLPT
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECC
WSEENIYATLKGLAAHLDIKMRDFMAPFFIAIAGSTSSTPVMNSMAIIGADMTLTRLRHA
CCCCCHHHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHEECCHHHHHHHHH
VDVLGGLGKKKLKKLEKQAAELPDFLAAE
HHHHHCCCHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MMQPSTSTNSIRPVRTRIAPSPTGFPHVGTAYIALFNLAFAKAHGGEFILRIEDTDQTRS
CCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
TEQSEKMILDALRWVGLDWAEGPDIGGPHAPYRQSERSDIYKKHAEQLIENDHAFRCFCS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
SEELDAMRAEQMANGETPRYDGRCAHLAAEKTEQLVAEGKPHVIRMRVPTEGVCQVQDML
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
RGTVEIPWTQVDMQVLLKTDGMPTYHLANVVDDHLMDISHVMRGEEWLNSAPKHQLLYEY
CCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
FGWEMPVLCHMPLLRNPDKSKLSKRKNPTSITYYRDAGVLPEALLNYLGRMGYSMPDEAE
HCCCCCHHHCCHHHCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHH
QFTLEEMIASFDIQRVSLGGPIFDIEKLNWLNSEWLRALTPEELKNKILEWASNSDKLTA
HHHHHHHHHHCCCEEEECCCCEEEHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCHHHH
IAAAIQPRIELLSDAVNWSGFYFQNLPDINAESFTHKSLTPEQIMDMLQLSLWQLEVLPT
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECC
WSEENIYATLKGLAAHLDIKMRDFMAPFFIAIAGSTSSTPVMNSMAIIGADMTLTRLRHA
CCCCCHHHHHHHHHHHCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHEECCHHHHHHHHH
VDVLGGLGKKKLKKLEKQAAELPDFLAAE
HHHHHCCCHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA