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The map label for this gene is cls [H]

Identifier: 93007197

GI number: 93007197

Start: 2916935

End: 2918374

Strand: Direct

Name: cls [H]

Synonym: Pcryo_2373

Alternate gene names: 93007197

Gene position: 2916935-2918374 (Clockwise)

Preceding gene: 93007196

Following gene: 93007205

Centisome position: 95.33

GC content: 43.75

Gene sequence:

>1440_bases
ATGATCGTAGATATTTTGTTGACGATACATTTTATCCTACTGATACTGATTTCTTTACGGGTCTTATCTCGTCATGATTT
GACCTCGCCCGCTAGATTAGCATGGATTGTCATTCTGTTTATTTTACCTTATTTTGGCGTGCTGATTTACTGGATGTTTG
GTGAAGTGCATTTGGGTCATGACTTTGGGCAAAAACGCGACGATATCATTAAGCGATTAAATACTCATAGTCCAAGCGTT
CTTGGTCATGAGACGACGTTAGAGAAGATGATCAAGCCCGCTTATCAAGCTTCCTTTGCTTATGCAGCAAAAGCCACCGG
TTTTCATACTATGGAGGGTAATCAGGCAGAGCTCATGGCAGATGCAAGCGAAACACGCAGACGCATGATTGCTGATTTTG
ATGCTGCAACAGACCATATCCATGTGCTGTATTACATTTGGCTCGTCGATGGCATGGGTATTGATACCGCGCAAGCTCTC
ATCCGAGCAGCAAAGCGCGGCGTTACTTGTCGAGCAATGGTTGATGGCATGGGCTCACGGAAGATGGTGAGCTCAAAGAT
ATGGCAAGAAATGCAAGATGCTGGCGTACAAGTCAGTGTAGCTCTCCCACTAACCAACTTTGTAAAAGTAATGCTGTTTA
GCCGTATTGATCTGCGTAATCACCGCAAAATTACCGTTATTGATGGCAAAATTGGCTACTCTGGTAGTCGTAACTGCGCC
GATCCTGAATTTATGGTAAAGCCAAAATATGCGCCGTGGGTCGATATTATGCTGCGTATCGAAGGACCTGTGGTCGCGCA
GAATCAGATGTTATTTGCTAGTGATTGGCTGATTGAAAACCCAGATACTTCGCTTGATAGCTTTTATTATGATAGCACGC
CAAAGCCTAATGGCTTTCCTGCCCAAGTTTTTTCAGATGGTCCTACTCAGCGGCACGGTACCACGCCACAGTTTTTGGGT
GCTTTGATTAGCCAAGCGCAAGAGACGCTGATTATCTCTACGCCTTATTTCGTGCCCGATTATTCACTCGTCAGTATTTT
ATGCGCTACTGCCTATCGCGGGGTTGAAGTGACGATGGTTTTTCCAAAAAATAACGATTCCTTTATCGTTGCCGCGACCA
GTCGCAGTTATTATTGGCAATTGCTCGAAGCGGGGGTAAAAATTTATGAATATAAACCAGGCTTGTTACACGCGAAGACC
TTGACGATTGATGGTGAAATTAGCTTGATTGGCTCGACCAATTTAGACTTACGTAGCTTTGATCTGAATTATGAGAATAA
CATTGTTTTTAGCGATAAAAAGCTGACGGCGGATATTATTGAGCGCCAATACCAATATATCGCTGACTCAGACGAAGTAA
CACGTGAAGAGGTCAAAAACTGGCCACTATCTTCTAGGATTTGGAACAACATCATTGCTACGATGGGTCCTGTTTTATAA

Upstream 100 bases:

>100_bases
GCTTTTTGGAGAATACTAGACGGTTTTCATAGTACTTCTCTTTTTGATTAGTTAAAGCGATTGATACTTTTTAGATAAAA
TAATAATAAGGGACGCTGTT

Downstream 100 bases:

>100_bases
GCCTCTTACTTTAAGCCCATCAGGTTATTTTGTGAAGTGTCTTGATAAGATGACATAGCGATAAGTAAACTTATAAGTTC
GTTGTTTTCTGCAAAATATA

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MIVDILLTIHFILLILISLRVLSRHDLTSPARLAWIVILFILPYFGVLIYWMFGEVHLGHDFGQKRDDIIKRLNTHSPSV
LGHETTLEKMIKPAYQASFAYAAKATGFHTMEGNQAELMADASETRRRMIADFDAATDHIHVLYYIWLVDGMGIDTAQAL
IRAAKRGVTCRAMVDGMGSRKMVSSKIWQEMQDAGVQVSVALPLTNFVKVMLFSRIDLRNHRKITVIDGKIGYSGSRNCA
DPEFMVKPKYAPWVDIMLRIEGPVVAQNQMLFASDWLIENPDTSLDSFYYDSTPKPNGFPAQVFSDGPTQRHGTTPQFLG
ALISQAQETLIISTPYFVPDYSLVSILCATAYRGVEVTMVFPKNNDSFIVAATSRSYYWQLLEAGVKIYEYKPGLLHAKT
LTIDGEISLIGSTNLDLRSFDLNYENNIVFSDKKLTADIIERQYQYIADSDEVTREEVKNWPLSSRIWNNIIATMGPVL

Sequences:

>Translated_479_residues
MIVDILLTIHFILLILISLRVLSRHDLTSPARLAWIVILFILPYFGVLIYWMFGEVHLGHDFGQKRDDIIKRLNTHSPSV
LGHETTLEKMIKPAYQASFAYAAKATGFHTMEGNQAELMADASETRRRMIADFDAATDHIHVLYYIWLVDGMGIDTAQAL
IRAAKRGVTCRAMVDGMGSRKMVSSKIWQEMQDAGVQVSVALPLTNFVKVMLFSRIDLRNHRKITVIDGKIGYSGSRNCA
DPEFMVKPKYAPWVDIMLRIEGPVVAQNQMLFASDWLIENPDTSLDSFYYDSTPKPNGFPAQVFSDGPTQRHGTTPQFLG
ALISQAQETLIISTPYFVPDYSLVSILCATAYRGVEVTMVFPKNNDSFIVAATSRSYYWQLLEAGVKIYEYKPGLLHAKT
LTIDGEISLIGSTNLDLRSFDLNYENNIVFSDKKLTADIIERQYQYIADSDEVTREEVKNWPLSSRIWNNIIATMGPVL
>Mature_479_residues
MIVDILLTIHFILLILISLRVLSRHDLTSPARLAWIVILFILPYFGVLIYWMFGEVHLGHDFGQKRDDIIKRLNTHSPSV
LGHETTLEKMIKPAYQASFAYAAKATGFHTMEGNQAELMADASETRRRMIADFDAATDHIHVLYYIWLVDGMGIDTAQAL
IRAAKRGVTCRAMVDGMGSRKMVSSKIWQEMQDAGVQVSVALPLTNFVKVMLFSRIDLRNHRKITVIDGKIGYSGSRNCA
DPEFMVKPKYAPWVDIMLRIEGPVVAQNQMLFASDWLIENPDTSLDSFYYDSTPKPNGFPAQVFSDGPTQRHGTTPQFLG
ALISQAQETLIISTPYFVPDYSLVSILCATAYRGVEVTMVFPKNNDSFIVAATSRSYYWQLLEAGVKIYEYKPGLLHAKT
LTIDGEISLIGSTNLDLRSFDLNYENNIVFSDKKLTADIIERQYQYIADSDEVTREEVKNWPLSSRIWNNIIATMGPVL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=33.5390946502058, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787007, Length=376, Percent_Identity=28.1914893617021, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081826, Length=403, Percent_Identity=27.2952853598015, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54074; Mature: 54074

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDILLTIHFILLILISLRVLSRHDLTSPARLAWIVILFILPYFGVLIYWMFGEVHLGH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
DFGQKRDDIIKRLNTHSPSVLGHETTLEKMIKPAYQASFAYAAKATGFHTMEGNQAELMA
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHEEE
DASETRRRMIADFDAATDHIHVLYYIWLVDGMGIDTAQALIRAAKRGVTCRAMVDGMGSR
CHHHHHHHHHHHCCCCCCHHEEEEEEEHHCCCCCHHHHHHHHHHHCCCEEEHHHCCCCCH
KMVSSKIWQEMQDAGVQVSVALPLTNFVKVMLFSRIDLRNHRKITVIDGKIGYSGSRNCA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
DPEFMVKPKYAPWVDIMLRIEGPVVAQNQMLFASDWLIENPDTSLDSFYYDSTPKPNGFP
CCCEEECCCCCCCEEEEEEECCCEEECCCEEEEECCEECCCCCCHHHEEECCCCCCCCCC
AQVFSDGPTQRHGTTPQFLGALISQAQETLIISTPYFVPDYSLVSILCATAYRGVEVTMV
CHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
FPKNNDSFIVAATSRSYYWQLLEAGVKIYEYKPGLLHAKTLTIDGEISLIGSTNLDLRSF
EECCCCCEEEEECCCHHHHHHHHCCCEEEEECCCEEEEEEEEECCEEEEEECCCCEEEEE
DLNYENNIVFSDKKLTADIIERQYQYIADSDEVTREEVKNWPLSSRIWNNIIATMGPVL
ECCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIVDILLTIHFILLILISLRVLSRHDLTSPARLAWIVILFILPYFGVLIYWMFGEVHLGH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
DFGQKRDDIIKRLNTHSPSVLGHETTLEKMIKPAYQASFAYAAKATGFHTMEGNQAELMA
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHEEE
DASETRRRMIADFDAATDHIHVLYYIWLVDGMGIDTAQALIRAAKRGVTCRAMVDGMGSR
CHHHHHHHHHHHCCCCCCHHEEEEEEEHHCCCCCHHHHHHHHHHHCCCEEEHHHCCCCCH
KMVSSKIWQEMQDAGVQVSVALPLTNFVKVMLFSRIDLRNHRKITVIDGKIGYSGSRNCA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
DPEFMVKPKYAPWVDIMLRIEGPVVAQNQMLFASDWLIENPDTSLDSFYYDSTPKPNGFP
CCCEEECCCCCCCEEEEEEECCCEEECCCEEEEECCEECCCCCCHHHEEECCCCCCCCCC
AQVFSDGPTQRHGTTPQFLGALISQAQETLIISTPYFVPDYSLVSILCATAYRGVEVTMV
CHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
FPKNNDSFIVAATSRSYYWQLLEAGVKIYEYKPGLLHAKTLTIDGEISLIGSTNLDLRSF
EECCCCCEEEEECCCHHHHHHHHCCCEEEEECCCEEEEEEEEECCEEEEEECCCCEEEEE
DLNYENNIVFSDKKLTADIIERQYQYIADSDEVTREEVKNWPLSSRIWNNIIATMGPVL
ECCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA