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The map label for this gene is murD [H]

Identifier: 93007167

GI number: 93007167

Start: 2873186

End: 2874601

Strand: Reverse

Name: murD [H]

Synonym: Pcryo_2343

Alternate gene names: 93007167

Gene position: 2874601-2873186 (Counterclockwise)

Preceding gene: 93007172

Following gene: 93007166

Centisome position: 93.95

GC content: 47.25

Gene sequence:

>1416_bases
ATGACCGCTAGCACCGCAACAGAAACCTTACTTCATAAAGGCAGTGGTTTACAAGTCGTGGTCGGTTTGGGGCAGTCGGG
GTTATCGGTCGCGCATTATTTGGCCGAGCAAGGCTACCAAGTTGCCGTCACTGACAATCAAGAAAATCCTGCGTTAGCAA
ATAAGCTACCTGCTACTATTGATATCAGGCAGTTTGGTGCGATCGATGCCGAATTATTACAACAAGCGGCACGGATTATT
ATCAGCCCTGGTATCTCTTTAGATAATGTCGCCATTGCCGCGGCACGAGCTGCAAATATTCCAGTGGTCAGTGATATTCA
GCTATTTTGTGAGGCTTGTACGGTACCAATCGTTGCGATTACAGGTTCAAATGCCAAGAGCACCGTTACGACACTGGTCG
GACAAATGGCGGCAGATGCAGGTATCAATGTTGGCGTAGGCGGTAATATTGGTGTGCCAGCGCTGAGCCTACTGAGTAAT
AAAGAGATGGAGCTGGCGGTTATAGAGTTATCCAGCTTTCAGTTAGAAACGGTCACCAACTTAGGAGCGCAGGTCGCGAC
AGTCCTCAATATGTCGCCTGATCACTTAGATCGTCATGGCGATATGCTCGGCTATCACCAAGCCAAACACCGTATCTTTC
AAGGTGCCAAATCAGTAGTGATTAATCGAGAAGATGCTTTGACGCGGCCACTGGTATCAGACAGTTTGCCAAGATTGAGT
ACTGGTATCCATGCGCCTAATAAAGGACATTATGGACTTATTACGGATACCGCCGGACAGACTTATTTAGCACGCGGCAC
AGAGCGTCTAATATCGGCAGACAAACTCAAGATAAAAGGGCGTCATAACCTGCTCAATGCCCAAGCAGCGTTGGCATTGG
GCGAGCTTGCTGGTTTGCCGCTTGAAATTATGCTCATTACTTTGCAGCAGTTTGCAGGGCTTGAGCATCGCTGCCAATAT
GTCTCTACAGTCGCTGGCATTGATTATTTTAATGACTCTAAAGGCACTAATATTGGCTCAACTATGGCGGCTGTTGAAGG
TTTAGGTGCAGTTTATGCGCCAAAAGACGGTAAGCTGCTATTAATATTGGGCGGTCAAGGTAAAGGTCAGCAGTTCGGTG
AACTTTCGCCCTTTATCAATCAATACGTGAGTCAAGTGCTGTTTATTGGTGAAGATGGTAAGCAGATTGAGCAGCATTTA
CGCGCAGCGGGTCTCAGCGATGAGGTCAGCCTACATCAATGTCAGACATTAGAAAATGCTTTTGCGACGATTGAGCAAGT
AACAGCAAGTAGTTTGTCGCAAGTACAGGCAGTGTTATTGTCACCTGCTTGTGCAAGCTTCGATCAGTTTGATGGGTTTG
TCGCGCGCGGCGAGCACTTTAGCCAGCTCGTTAAACAATTGGATGTTGTGTCTTAA

Upstream 100 bases:

>100_bases
TTTAATCAAATGGTTCAGGCGATATGTGCAGTACATTGATTGTCTGTTTTCTTGATATCATTTTGTTTATTTATTTGTTC
GTTTCATAAAAGGAAAAGCC

Downstream 100 bases:

>100_bases
AGGTTTCGATCATGAACGGCAAAACCTAAGCATCTATATAGCCCTATTCGGTCTTATAAAGCGCCTTGTTACAATATTAT
GTGACCTGCGCTTTATAATC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTASTATETLLHKGSGLQVVVGLGQSGLSVAHYLAEQGYQVAVTDNQENPALANKLPATIDIRQFGAIDAELLQQAARII
ISPGISLDNVAIAAARAANIPVVSDIQLFCEACTVPIVAITGSNAKSTVTTLVGQMAADAGINVGVGGNIGVPALSLLSN
KEMELAVIELSSFQLETVTNLGAQVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAKSVVINREDALTRPLVSDSLPRLS
TGIHAPNKGHYGLITDTAGQTYLARGTERLISADKLKIKGRHNLLNAQAALALGELAGLPLEIMLITLQQFAGLEHRCQY
VSTVAGIDYFNDSKGTNIGSTMAAVEGLGAVYAPKDGKLLLILGGQGKGQQFGELSPFINQYVSQVLFIGEDGKQIEQHL
RAAGLSDEVSLHQCQTLENAFATIEQVTASSLSQVQAVLLSPACASFDQFDGFVARGEHFSQLVKQLDVVS

Sequences:

>Translated_471_residues
MTASTATETLLHKGSGLQVVVGLGQSGLSVAHYLAEQGYQVAVTDNQENPALANKLPATIDIRQFGAIDAELLQQAARII
ISPGISLDNVAIAAARAANIPVVSDIQLFCEACTVPIVAITGSNAKSTVTTLVGQMAADAGINVGVGGNIGVPALSLLSN
KEMELAVIELSSFQLETVTNLGAQVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAKSVVINREDALTRPLVSDSLPRLS
TGIHAPNKGHYGLITDTAGQTYLARGTERLISADKLKIKGRHNLLNAQAALALGELAGLPLEIMLITLQQFAGLEHRCQY
VSTVAGIDYFNDSKGTNIGSTMAAVEGLGAVYAPKDGKLLLILGGQGKGQQFGELSPFINQYVSQVLFIGEDGKQIEQHL
RAAGLSDEVSLHQCQTLENAFATIEQVTASSLSQVQAVLLSPACASFDQFDGFVARGEHFSQLVKQLDVVS
>Mature_470_residues
TASTATETLLHKGSGLQVVVGLGQSGLSVAHYLAEQGYQVAVTDNQENPALANKLPATIDIRQFGAIDAELLQQAARIII
SPGISLDNVAIAAARAANIPVVSDIQLFCEACTVPIVAITGSNAKSTVTTLVGQMAADAGINVGVGGNIGVPALSLLSNK
EMELAVIELSSFQLETVTNLGAQVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAKSVVINREDALTRPLVSDSLPRLST
GIHAPNKGHYGLITDTAGQTYLARGTERLISADKLKIKGRHNLLNAQAALALGELAGLPLEIMLITLQQFAGLEHRCQYV
STVAGIDYFNDSKGTNIGSTMAAVEGLGAVYAPKDGKLLLILGGQGKGQQFGELSPFINQYVSQVLFIGEDGKQIEQHLR
AAGLSDEVSLHQCQTLENAFATIEQVTASSLSQVQAVLLSPACASFDQFDGFVARGEHFSQLVKQLDVVS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=450, Percent_Identity=44.2222222222222, Blast_Score=311, Evalue=8e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49679; Mature: 49548

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTATETLLHKGSGLQVVVGLGQSGLSVAHYLAEQGYQVAVTDNQENPALANKLPATI
CCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCEE
DIRQFGAIDAELLQQAARIIISPGISLDNVAIAAARAANIPVVSDIQLFCEACTVPIVAI
EHHHHCCCCHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEE
TGSNAKSTVTTLVGQMAADAGINVGVGGNIGVPALSLLSNKEMELAVIELSSFQLETVTN
ECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHCCCCCEEEEEEECCCHHHHHHH
LGAQVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAKSVVINREDALTRPLVSDSLPRLS
CCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCHHEEEECHHHHHCCHHHCCCCHHH
TGIHAPNKGHYGLITDTAGQTYLARGTERLISADKLKIKGRHNLLNAQAALALGELAGLP
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCC
LEIMLITLQQFAGLEHRCQYVSTVAGIDYFNDSKGTNIGSTMAAVEGLGAVYAPKDGKLL
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCEE
LILGGQGKGQQFGELSPFINQYVSQVLFIGEDGKQIEQHLRAAGLSDEVSLHQCQTLENA
EEECCCCCCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FATIEQVTASSLSQVQAVLLSPACASFDQFDGFVARGEHFSQLVKQLDVVS
HHHHHHHHHHHHHHHHHHHHCHHHCCHHHHCCHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASTATETLLHKGSGLQVVVGLGQSGLSVAHYLAEQGYQVAVTDNQENPALANKLPATI
CCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCEE
DIRQFGAIDAELLQQAARIIISPGISLDNVAIAAARAANIPVVSDIQLFCEACTVPIVAI
EHHHHCCCCHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEE
TGSNAKSTVTTLVGQMAADAGINVGVGGNIGVPALSLLSNKEMELAVIELSSFQLETVTN
ECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHCCCCCEEEEEEECCCHHHHHHH
LGAQVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAKSVVINREDALTRPLVSDSLPRLS
CCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCHHEEEECHHHHHCCHHHCCCCHHH
TGIHAPNKGHYGLITDTAGQTYLARGTERLISADKLKIKGRHNLLNAQAALALGELAGLP
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCC
LEIMLITLQQFAGLEHRCQYVSTVAGIDYFNDSKGTNIGSTMAAVEGLGAVYAPKDGKLL
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCEE
LILGGQGKGQQFGELSPFINQYVSQVLFIGEDGKQIEQHLRAAGLSDEVSLHQCQTLENA
EEECCCCCCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FATIEQVTASSLSQVQAVLLSPACASFDQFDGFVARGEHFSQLVKQLDVVS
HHHHHHHHHHHHHHHHHHHHCHHHCCHHHHCCHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA