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The map label for this gene is yiaE [C]

Identifier: 93007113

GI number: 93007113

Start: 2815936

End: 2816874

Strand: Direct

Name: yiaE [C]

Synonym: Pcryo_2289

Alternate gene names: 93007113

Gene position: 2815936-2816874 (Clockwise)

Preceding gene: 93007112

Following gene: 93007116

Centisome position: 92.03

GC content: 44.94

Gene sequence:

>939_bases
ATGCAAGCGGTTTTTTTAGATAAAGGCACTTTTTCTGATAACGTCGATTTGCCAGCACCCGCTGGCGTTACTGATTACAT
TACTTACGATGATACGCCTCAAGCACTGATCATTGAGCGCTGCAAAGACGCCGATATTATTTTTACTAATAAAGTGCAAA
TCAATGCCGAAGCGATTGCTAATTTGCCAAAGCTAAAACTTATTCAACTGACCGCTACGGGTATGAATAACGTTGACCAA
GATGCTTGTGCTGAGCATAATATCGCGCTCTATAACGTCGCCGGTTACGCGGTGAAAAGCGTGCCTGAGCATACCTTTAT
GCTGATGCTAAATGCCATGCGTGCCGGTCTGCATTATCATAATAAAGTCACCGATGGCACGTGGGCAGATAATGGTAATT
TTTGCCTACTTGATATCCCGCTTATAGATTTGGAAAATAAAACGCTGGGTATTATTGGCATTGGTACCATTGGCAAACGT
GTCACTGATATTGCGCGTGCTTTTGGCATGACGGTATTGTGGGCGGAGCACCAAGGGCGTATGCCGCGTAATGATGACTA
TACAGCGTTTGAAGACGTACTGGCGCAGTCCGATGTACTGAGCTTGCATTGCCCGTTAAATGAGAAAACTCAGCACCTTA
TTAATGCAGACACCTTGGCCAAAATGACTAAAAAACCTCTACTTGTCAACGTCGCTCGTGGTGGTATTGTGGATAGCCAA
GCGCTTGCTGATGCCATTAACAATGAGCAGATTTTAGGGTATGGCAGCGACGTTTTTGAGCAAGAACCCATAGCTGCCAA
TGATCCTTTACTGGCTCTTGGTGAGCATCCACGCGTGATATTTAGTCCGCACAATGCGTGGGGCAGCAAAAGCGCGCAAG
AAACCTTATGGCAAATGCTTAGTAAACAAGTCAGTGATTTTATCAATCAAGCTCAGTAA

Upstream 100 bases:

>100_bases
GCGCTTTTTGACATGTTTTTTGACTAAATGCAGCAACGTGCTACATTAAGCCGATAATAAACAAAACCTATTTACGATAA
CAACGAGATAAGGAATCATC

Downstream 100 bases:

>100_bases
TATTAATAATTAGCAAATTCTAACGATTAAACTGTAGCCACTCATCTTTGATTTTACGGTATTTGAGCATGATGCGATCG
GTGACATAGTTGTTGTTCAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQAVFLDKGTFSDNVDLPAPAGVTDYITYDDTPQALIIERCKDADIIFTNKVQINAEAIANLPKLKLIQLTATGMNNVDQ
DACAEHNIALYNVAGYAVKSVPEHTFMLMLNAMRAGLHYHNKVTDGTWADNGNFCLLDIPLIDLENKTLGIIGIGTIGKR
VTDIARAFGMTVLWAEHQGRMPRNDDYTAFEDVLAQSDVLSLHCPLNEKTQHLINADTLAKMTKKPLLVNVARGGIVDSQ
ALADAINNEQILGYGSDVFEQEPIAANDPLLALGEHPRVIFSPHNAWGSKSAQETLWQMLSKQVSDFINQAQ

Sequences:

>Translated_312_residues
MQAVFLDKGTFSDNVDLPAPAGVTDYITYDDTPQALIIERCKDADIIFTNKVQINAEAIANLPKLKLIQLTATGMNNVDQ
DACAEHNIALYNVAGYAVKSVPEHTFMLMLNAMRAGLHYHNKVTDGTWADNGNFCLLDIPLIDLENKTLGIIGIGTIGKR
VTDIARAFGMTVLWAEHQGRMPRNDDYTAFEDVLAQSDVLSLHCPLNEKTQHLINADTLAKMTKKPLLVNVARGGIVDSQ
ALADAINNEQILGYGSDVFEQEPIAANDPLLALGEHPRVIFSPHNAWGSKSAQETLWQMLSKQVSDFINQAQ
>Mature_312_residues
MQAVFLDKGTFSDNVDLPAPAGVTDYITYDDTPQALIIERCKDADIIFTNKVQINAEAIANLPKLKLIQLTATGMNNVDQ
DACAEHNIALYNVAGYAVKSVPEHTFMLMLNAMRAGLHYHNKVTDGTWADNGNFCLLDIPLIDLENKTLGIIGIGTIGKR
VTDIARAFGMTVLWAEHQGRMPRNDDYTAFEDVLAQSDVLSLHCPLNEKTQHLINADTLAKMTKKPLLVNVARGGIVDSQ
ALADAINNEQILGYGSDVFEQEPIAANDPLLALGEHPRVIFSPHNAWGSKSAQETLWQMLSKQVSDFINQAQ

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=269, Percent_Identity=31.5985130111524, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557499, Length=269, Percent_Identity=31.5985130111524, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=30, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=30, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580575, Length=248, Percent_Identity=33.8709677419355, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI23308577, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI6912396, Length=239, Percent_Identity=25.9414225941423, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082289, Length=276, Percent_Identity=30.7971014492754, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787645, Length=256, Percent_Identity=28.90625, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789279, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081824, Length=248, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=284, Percent_Identity=30.2816901408451, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=273, Percent_Identity=27.1062271062271, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=29.6296296296296, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=265, Percent_Identity=29.4339622641509, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=291, Percent_Identity=26.4604810996564, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=291, Percent_Identity=26.4604810996564, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=146, Percent_Identity=30.8219178082192, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646446, Length=269, Percent_Identity=32.7137546468402, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646448, Length=269, Percent_Identity=32.7137546468402, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646452, Length=269, Percent_Identity=32.7137546468402, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24646450, Length=269, Percent_Identity=32.7137546468402, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI62472511, Length=269, Percent_Identity=32.7137546468402, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921140, Length=257, Percent_Identity=29.5719844357977, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=28.8808664259928, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28571528, Length=227, Percent_Identity=27.3127753303965, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585514, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574282, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28574284, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45552429, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45551003, Length=268, Percent_Identity=26.4925373134328, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=301, Percent_Identity=25.9136212624585, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34242; Mature: 34242

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVFLDKGTFSDNVDLPAPAGVTDYITYDDTPQALIIERCKDADIIFTNKVQINAEAIA
CCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEECHHHHC
NLPKLKLIQLTATGMNNVDQDACAEHNIALYNVAGYAVKSVPEHTFMLMLNAMRAGLHYH
CCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHC
NKVTDGTWADNGNFCLLDIPLIDLENKTLGIIGIGTIGKRVTDIARAFGMTVLWAEHQGR
CCCCCCEECCCCCEEEEEEEEEEECCCEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCC
MPRNDDYTAFEDVLAQSDVLSLHCPLNEKTQHLINADTLAKMTKKPLLVNVARGGIVDSQ
CCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHHHCCCEEEEECCCCCCCHH
ALADAINNEQILGYGSDVFEQEPIAANDPLLALGEHPRVIFSPHNAWGSKSAQETLWQML
HHHHHCCCCEEEECCCHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHH
SKQVSDFINQAQ
HHHHHHHHHHCC
>Mature Secondary Structure
MQAVFLDKGTFSDNVDLPAPAGVTDYITYDDTPQALIIERCKDADIIFTNKVQINAEAIA
CCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEECHHHHC
NLPKLKLIQLTATGMNNVDQDACAEHNIALYNVAGYAVKSVPEHTFMLMLNAMRAGLHYH
CCCCEEEEEEEECCCCCCCHHHHHHCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHC
NKVTDGTWADNGNFCLLDIPLIDLENKTLGIIGIGTIGKRVTDIARAFGMTVLWAEHQGR
CCCCCCEECCCCCEEEEEEEEEEECCCEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCC
MPRNDDYTAFEDVLAQSDVLSLHCPLNEKTQHLINADTLAKMTKKPLLVNVARGGIVDSQ
CCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHHHCCCEEEEECCCCCCCHH
ALADAINNEQILGYGSDVFEQEPIAANDPLLALGEHPRVIFSPHNAWGSKSAQETLWQML
HHHHHCCCCEEEECCCHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHH
SKQVSDFINQAQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]