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The map label for this gene is murI [H]

Identifier: 93007073

GI number: 93007073

Start: 2758459

End: 2759460

Strand: Reverse

Name: murI [H]

Synonym: Pcryo_2249

Alternate gene names: 93007073

Gene position: 2759460-2758459 (Counterclockwise)

Preceding gene: 93007074

Following gene: 93007072

Centisome position: 90.18

GC content: 42.51

Gene sequence:

>1002_bases
ATGAAAGTTGCCGCAAGTGCTGTTACTAACACAGTTAATACTTACTTAATAATTTCTGATAATAACACTGCTGAAGAAGC
GATGGTCGATAGTCTGATATACAATCATAATAAAGGGACAAAGGCAGATCCAGACCGTAGCGCGCCTATTGGCTTATTTG
ATTCCGGTATTGGTGGCTTGTCTGTTTATCTCCATTTAGCCCAGCAACTGCCCAATGAACGTTATATTTACTATGCTGAT
ACCCTGAATGTGCCCTATGGCAATCGAGATAGTGATGATATTAAAACATTCACATTGACGGCAGTAGAGTGGCTATATCA
GCAAGGCTGCAAGCTTATTGTCATTGCGTGTAATAGTGCTTCAGCCTATGCGTTGCAGACAGCAAGGTGCCATTATCCGC
AGCTACCCATCGTGGGTTTGGTACCAGCATTGAAACCTGCGGTATTGGCCAGTAAAAGTGGTCATGTGGCGGTACTAGCA
ACCAAAGCGACTCTAAACGGTAAACTCCTAAACGATGTCATTGCTACGATAGCCAGTCCAAGTGCAACCAGAGTGACCAA
ATATTTTGACCCGCAACTTGTGCCTTGGGTAGAATCAGGTATGCCGGAAAACAGTGAAACGGCGCAAAATCTTCGTCAGC
AATTGCAAGTGTTCGCAAAGGATGGGGTAGACCAGCTGGTATTAGGCTGTACACATTATCCATTCTTTAAAGATTTTGTG
ATGCAAGCAATTGAATCGCAAAGACTATCAATGCAAGTGGTTGATTCAGGTCAAGCAATTGCAGAGCGCGTTAAGCAATT
GTTGATCGCCAATAATTTGTCAGCATTGCCTATAGATGGATTAGATAGAAATGGTGTTTTAGATGCCAGTTTGAGTGACA
ATCAGATTAAGCCACCGTTGATATTTTATGCGACCAAATACGATAAGCATATGGATTTGCTGCTATCACGCTTACTTGGC
GAGCAAACGCATTTACAATACCATCCTCATTTTGAGTATTAG

Upstream 100 bases:

>100_bases
GTTTTATTTGGAATTGACTATATTATCGAAAGTAATATTGGTTAAATTTATTAGTAGGGTCGGTATGACAGTATTTTTAT
CAAAATGGCGGTGAAATAAT

Downstream 100 bases:

>100_bases
CCTTAGGGGCTCCTAGGATTAATTTCGATACAGCTGTTATAATCCCGTAGTATATTAATGGAATTGGAAATAAAGTAAAC
GTATTCTAGCAATATTATGC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKVAASAVTNTVNTYLIISDNNTAEEAMVDSLIYNHNKGTKADPDRSAPIGLFDSGIGGLSVYLHLAQQLPNERYIYYAD
TLNVPYGNRDSDDIKTFTLTAVEWLYQQGCKLIVIACNSASAYALQTARCHYPQLPIVGLVPALKPAVLASKSGHVAVLA
TKATLNGKLLNDVIATIASPSATRVTKYFDPQLVPWVESGMPENSETAQNLRQQLQVFAKDGVDQLVLGCTHYPFFKDFV
MQAIESQRLSMQVVDSGQAIAERVKQLLIANNLSALPIDGLDRNGVLDASLSDNQIKPPLIFYATKYDKHMDLLLSRLLG
EQTHLQYHPHFEY

Sequences:

>Translated_333_residues
MKVAASAVTNTVNTYLIISDNNTAEEAMVDSLIYNHNKGTKADPDRSAPIGLFDSGIGGLSVYLHLAQQLPNERYIYYAD
TLNVPYGNRDSDDIKTFTLTAVEWLYQQGCKLIVIACNSASAYALQTARCHYPQLPIVGLVPALKPAVLASKSGHVAVLA
TKATLNGKLLNDVIATIASPSATRVTKYFDPQLVPWVESGMPENSETAQNLRQQLQVFAKDGVDQLVLGCTHYPFFKDFV
MQAIESQRLSMQVVDSGQAIAERVKQLLIANNLSALPIDGLDRNGVLDASLSDNQIKPPLIFYATKYDKHMDLLLSRLLG
EQTHLQYHPHFEY
>Mature_333_residues
MKVAASAVTNTVNTYLIISDNNTAEEAMVDSLIYNHNKGTKADPDRSAPIGLFDSGIGGLSVYLHLAQQLPNERYIYYAD
TLNVPYGNRDSDDIKTFTLTAVEWLYQQGCKLIVIACNSASAYALQTARCHYPQLPIVGLVPALKPAVLASKSGHVAVLA
TKATLNGKLLNDVIATIASPSATRVTKYFDPQLVPWVESGMPENSETAQNLRQQLQVFAKDGVDQLVLGCTHYPFFKDFV
MQAIESQRLSMQVVDSGQAIAERVKQLLIANNLSALPIDGLDRNGVLDASLSDNQIKPPLIFYATKYDKHMDLLLSRLLG
EQTHLQYHPHFEY

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=234, Percent_Identity=35.042735042735, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 36671; Mature: 36671

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00923 ASP_GLU_RACEMASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAASAVTNTVNTYLIISDNNTAEEAMVDSLIYNHNKGTKADPDRSAPIGLFDSGIGGL
CCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHH
SVYLHLAQQLPNERYIYYADTLNVPYGNRDSDDIKTFTLTAVEWLYQQGCKLIVIACNSA
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
SAYALQTARCHYPQLPIVGLVPALKPAVLASKSGHVAVLATKATLNGKLLNDVIATIASP
CCEEEEHHCCCCCCCCHHHHHHHHCHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCC
SATRVTKYFDPQLVPWVESGMPENSETAQNLRQQLQVFAKDGVDQLVLGCTHYPFFKDFV
CHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
MQAIESQRLSMQVVDSGQAIAERVKQLLIANNLSALPIDGLDRNGVLDASLSDNQIKPPL
HHHHHHHHCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCE
IFYATKYDKHMDLLLSRLLGEQTHLQYHPHFEY
EEEEECCCHHHHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MKVAASAVTNTVNTYLIISDNNTAEEAMVDSLIYNHNKGTKADPDRSAPIGLFDSGIGGL
CCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHH
SVYLHLAQQLPNERYIYYADTLNVPYGNRDSDDIKTFTLTAVEWLYQQGCKLIVIACNSA
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
SAYALQTARCHYPQLPIVGLVPALKPAVLASKSGHVAVLATKATLNGKLLNDVIATIASP
CCEEEEHHCCCCCCCCHHHHHHHHCHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCC
SATRVTKYFDPQLVPWVESGMPENSETAQNLRQQLQVFAKDGVDQLVLGCTHYPFFKDFV
CHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
MQAIESQRLSMQVVDSGQAIAERVKQLLIANNLSALPIDGLDRNGVLDASLSDNQIKPPL
HHHHHHHHCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCE
IFYATKYDKHMDLLLSRLLGEQTHLQYHPHFEY
EEEEECCCHHHHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]