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The map label for this gene is pyrE [H]

Identifier: 93006858

GI number: 93006858

Start: 2500227

End: 2500931

Strand: Reverse

Name: pyrE [H]

Synonym: Pcryo_2034

Alternate gene names: 93006858

Gene position: 2500931-2500227 (Counterclockwise)

Preceding gene: 93006859

Following gene: 93006857

Centisome position: 81.73

GC content: 46.81

Gene sequence:

>705_bases
ATGCCCCATGCCCAATACGCTAACAATGCCCCATTTTCATCTCATGCCTTTATTCAACTGGCGTTAGACAACCAAGTCTT
AAAGTTTGGCGAGTTTGTCTTAAAGTCTGGTCGCATTAGTCCGTATTTTTTTAATGCAGGTCTGCTTGCATCAGGTGAGA
TGTTGTCGCTATTAGCACGTGGTTATGCAGATGCCTTAGCTGAACAGATGGCTGCTACAAATGAGGGTAAAGGCGCACAA
GAATTGGTTATCTTTGGTGCCGCTTATAAGGGTATTCCCTTTGTGGCAGCAACTGCACAGGCTTTATGGTTACATCATGG
TATCAATGCCAACTGGGGTTACAACCGTAAAGAAGCCAAAACACACGGTGAAGGTGGTAATTTGGTCGGCGCTGACGTCA
GTGGCAAGGCGGTTTGGGTATTGGATGATGTCATTACCGCTGGTACAGCGATGCGTGAAGTGGTTGAGATTTTAAAACAA
GCAGGCGCACATGTTGCTGGTATTATTGTGGCGCTTGACCGTAAGGAAAAAGGCTTAGCAGAGCGCTCGGCAATTCAGGA
ACTGGCGGCAACGTTAAACGTTCCAGTGCGCGCCCTTGTCGATATTGACGACTTAATCAGCTATCTGGCAGAGGGTGGTC
ATTCGAATCAAAATGCAACCCAACTTGCAAAAATGCAGCATTACCGTGGTCAATATGGCGTATAG

Upstream 100 bases:

>100_bases
ATTATGATATCAATGGTTGGTCAAGTTAAGCGCCAACGCCTATAATGCGCGTCATGATAATCATTTTTAAACCTTTCTTT
TTGTTCATAGGAATTCTCTA

Downstream 100 bases:

>100_bases
TTAATCGAAACCATTGCTAACTATATTTGCCTATATTGGCATGCTGATATGTGATTTGTGTGTATTTTATCTATTAATAA
CATCCTGTAAATGAGTATTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MPHAQYANNAPFSSHAFIQLALDNQVLKFGEFVLKSGRISPYFFNAGLLASGEMLSLLARGYADALAEQMAATNEGKGAQ
ELVIFGAAYKGIPFVAATAQALWLHHGINANWGYNRKEAKTHGEGGNLVGADVSGKAVWVLDDVITAGTAMREVVEILKQ
AGAHVAGIIVALDRKEKGLAERSAIQELAATLNVPVRALVDIDDLISYLAEGGHSNQNATQLAKMQHYRGQYGV

Sequences:

>Translated_234_residues
MPHAQYANNAPFSSHAFIQLALDNQVLKFGEFVLKSGRISPYFFNAGLLASGEMLSLLARGYADALAEQMAATNEGKGAQ
ELVIFGAAYKGIPFVAATAQALWLHHGINANWGYNRKEAKTHGEGGNLVGADVSGKAVWVLDDVITAGTAMREVVEILKQ
AGAHVAGIIVALDRKEKGLAERSAIQELAATLNVPVRALVDIDDLISYLAEGGHSNQNATQLAKMQHYRGQYGV
>Mature_233_residues
PHAQYANNAPFSSHAFIQLALDNQVLKFGEFVLKSGRISPYFFNAGLLASGEMLSLLARGYADALAEQMAATNEGKGAQE
LVIFGAAYKGIPFVAATAQALWLHHGINANWGYNRKEAKTHGEGGNLVGADVSGKAVWVLDDVITAGTAMREVVEILKQA
GAHVAGIIVALDRKEKGLAERSAIQELAATLNVPVRALVDIDDLISYLAEGGHSNQNATQLAKMQHYRGQYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=160, Percent_Identity=30, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790073, Length=218, Percent_Identity=50.4587155963303, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323530, Length=227, Percent_Identity=41.8502202643172, Blast_Score=155, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6323927, Length=227, Percent_Identity=39.2070484581498, Blast_Score=152, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 24987; Mature: 24856

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAQYANNAPFSSHAFIQLALDNQVLKFGEFVLKSGRISPYFFNAGLLASGEMLSLLAR
CCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
GYADALAEQMAATNEGKGAQELVIFGAAYKGIPFVAATAQALWLHHGINANWGYNRKEAK
HHHHHHHHHHHHCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHC
THGEGGNLVGADVSGKAVWVLDDVITAGTAMREVVEILKQAGAHVAGIIVALDRKEKGLA
CCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ERSAIQELAATLNVPVRALVDIDDLISYLAEGGHSNQNATQLAKMQHYRGQYGV
HHHHHHHHHHHHCCCHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PHAQYANNAPFSSHAFIQLALDNQVLKFGEFVLKSGRISPYFFNAGLLASGEMLSLLAR
CCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
GYADALAEQMAATNEGKGAQELVIFGAAYKGIPFVAATAQALWLHHGINANWGYNRKEAK
HHHHHHHHHHHHCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHC
THGEGGNLVGADVSGKAVWVLDDVITAGTAMREVVEILKQAGAHVAGIIVALDRKEKGLA
CCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ERSAIQELAATLNVPVRALVDIDDLISYLAEGGHSNQNATQLAKMQHYRGQYGV
HHHHHHHHHHHHCCCHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA