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The map label for this gene is gshB [H]

Identifier: 93006857

GI number: 93006857

Start: 2499153

End: 2500136

Strand: Reverse

Name: gshB [H]

Synonym: Pcryo_2033

Alternate gene names: 93006857

Gene position: 2500136-2499153 (Counterclockwise)

Preceding gene: 93006858

Following gene: 93006856

Centisome position: 81.71

GC content: 44.41

Gene sequence:

>984_bases
ATGAGTATTCTTATGAGCCTAGAACAGCCAAAAAAATCTTTGAACATACTTGTGATTATGGATCCCATTGAGCAAGTTAA
TTATAAAAAAGATACTAGCCTTGCTATGATGTGGTCGGCGCAAGATCGCGGTCATCGTTTGGGTTACTGTCAGATTCATG
ATTTATGGCTTGATCGTGGGCAGTTGATGATCGATACGCAGTCAGTTACTGTCAAGCGTGATCCTGAGGATTTTTATACG
TTAGGGGATAAGACGACAGGTTCGGTCAGCGATTATGATGTGATTTTGATGCGTAAAGATCCACCGTTTGACATGCGCTT
TATTTATGCAACTTATATGCTTGACCATGCAAAAGCAGCAGGCGTATTAGTCGTCAATGACCCACAAGCCATTCGTGATT
GTAATGAGAAATTGTTTGCCACTTGGTTTAGCGACTATATGAGCCCAACGATTGTGACCAGTAAACAGGCTCACATTCGC
AAATTTATCGCTGAGCATCAAGACGTTATTGTCAAGCCATTAGATGGTATGGGCGGTACGGGTATATTTCGTTTGACTGC
TGATAGCCCAAATATTGGCGTGACGCTTGAGATGTTGACTGAGCTTGAAACGCTGCCGATTATGGCGCAGCGTTATCTAC
CAGAGATTAAAGAAGGTGATAAGCGCGTACTTATTGTCGATGGCACTGTGGTTGACTATACATTGGCTCGTATTCCAACA
AAGGGTGAAACGCGTGGGAATCTTGCCGCTGGTGGTAGCGGTGTAGCCATGCCGCTGACCGATATCGAACGCCAAGTCGC
AGAAGTGGTCGCGTCTATTGTCAAAGCAAAAGGTTTAATGTTCGTTGGTTTAGACCTAATTGGCGGACGCATTACGGAAA
TTAATGTCACCAGTCCAACCTGTGTGCGTGAGATTGATGACCAATGTGGTACAGACATTGCGACCGATTTTATCATCGCA
ATAGAGCGAAGACTGGCAATCTGA

Upstream 100 bases:

>100_bases
TGGCGTATAGTTAATCGAAACCATTGCTAACTATATTTGCCTATATTGGCATGCTGATATGTGATTTGTGTGTATTTTAT
CTATTAATAACATCCTGTAA

Downstream 100 bases:

>100_bases
TAGGCTGCTAAAGTTTGTTAGTTAAATAAGCTGTAAGGATAGAGTAATGTAATGCTCATCTACTGGCACAATGAGTGAAA
ATAGTTGTGCTAACATAGAT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSILMSLEQPKKSLNILVIMDPIEQVNYKKDTSLAMMWSAQDRGHRLGYCQIHDLWLDRGQLMIDTQSVTVKRDPEDFYT
LGDKTTGSVSDYDVILMRKDPPFDMRFIYATYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDYMSPTIVTSKQAHIR
KFIAEHQDVIVKPLDGMGGTGIFRLTADSPNIGVTLEMLTELETLPIMAQRYLPEIKEGDKRVLIVDGTVVDYTLARIPT
KGETRGNLAAGGSGVAMPLTDIERQVAEVVASIVKAKGLMFVGLDLIGGRITEINVTSPTCVREIDDQCGTDIATDFIIA
IERRLAI

Sequences:

>Translated_327_residues
MSILMSLEQPKKSLNILVIMDPIEQVNYKKDTSLAMMWSAQDRGHRLGYCQIHDLWLDRGQLMIDTQSVTVKRDPEDFYT
LGDKTTGSVSDYDVILMRKDPPFDMRFIYATYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDYMSPTIVTSKQAHIR
KFIAEHQDVIVKPLDGMGGTGIFRLTADSPNIGVTLEMLTELETLPIMAQRYLPEIKEGDKRVLIVDGTVVDYTLARIPT
KGETRGNLAAGGSGVAMPLTDIERQVAEVVASIVKAKGLMFVGLDLIGGRITEINVTSPTCVREIDDQCGTDIATDFIIA
IERRLAI
>Mature_326_residues
SILMSLEQPKKSLNILVIMDPIEQVNYKKDTSLAMMWSAQDRGHRLGYCQIHDLWLDRGQLMIDTQSVTVKRDPEDFYTL
GDKTTGSVSDYDVILMRKDPPFDMRFIYATYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDYMSPTIVTSKQAHIRK
FIAEHQDVIVKPLDGMGGTGIFRLTADSPNIGVTLEMLTELETLPIMAQRYLPEIKEGDKRVLIVDGTVVDYTLARIPTK
GETRGNLAAGGSGVAMPLTDIERQVAEVVASIVKAKGLMFVGLDLIGGRITEINVTSPTCVREIDDQCGTDIATDFIIAI
ERRLAI

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=308, Percent_Identity=52.9220779220779, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36391; Mature: 36259

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILMSLEQPKKSLNILVIMDPIEQVNYKKDTSLAMMWSAQDRGHRLGYCQIHDLWLDRG
CCEEEECCCCCCCCEEEEEECCHHHCCCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCC
QLMIDTQSVTVKRDPEDFYTLGDKTTGSVSDYDVILMRKDPPFDMRFIYATYMLDHAKAA
EEEEEECEEEEEECCHHHEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
GVLVVNDPQAIRDCNEKLFATWFSDYMSPTIVTSKQAHIRKFIAEHQDVIVKPLDGMGGT
CEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCC
GIFRLTADSPNIGVTLEMLTELETLPIMAQRYLPEIKEGDKRVLIVDGTVVDYTLARIPT
EEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCEEEEHHHHCCC
KGETRGNLAAGGSGVAMPLTDIERQVAEVVASIVKAKGLMFVGLDLIGGRITEINVTSPT
CCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCH
CVREIDDQCGTDIATDFIIAIERRLAI
HHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SILMSLEQPKKSLNILVIMDPIEQVNYKKDTSLAMMWSAQDRGHRLGYCQIHDLWLDRG
CEEEECCCCCCCCEEEEEECCHHHCCCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCC
QLMIDTQSVTVKRDPEDFYTLGDKTTGSVSDYDVILMRKDPPFDMRFIYATYMLDHAKAA
EEEEEECEEEEEECCHHHEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
GVLVVNDPQAIRDCNEKLFATWFSDYMSPTIVTSKQAHIRKFIAEHQDVIVKPLDGMGGT
CEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCC
GIFRLTADSPNIGVTLEMLTELETLPIMAQRYLPEIKEGDKRVLIVDGTVVDYTLARIPT
EEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCEEEEHHHHCCC
KGETRGNLAAGGSGVAMPLTDIERQVAEVVASIVKAKGLMFVGLDLIGGRITEINVTSPT
CCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCH
CVREIDDQCGTDIATDFIIAIERRLAI
HHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]