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The map label for this gene is rlmE [H]

Identifier: 93006845

GI number: 93006845

Start: 2485356

End: 2485988

Strand: Reverse

Name: rlmE [H]

Synonym: Pcryo_2021

Alternate gene names: 93006845

Gene position: 2485988-2485356 (Counterclockwise)

Preceding gene: 93006848

Following gene: 93006844

Centisome position: 81.24

GC content: 44.71

Gene sequence:

>633_bases
TTGGCCACACGTATTGAAAACAAAAAATTGTCAAAGAGCAGTAGTGCTTGGATGAAAGAGCACATTGATGATCATTATGT
GCAAAAGGCCCAAAAAGATGGCTACCGTGCCCGTGCTGCTTATAAATTATTAGAAATAAATGAAAAAACCAATCTGATCA
AAAAAGGTATGACCGTTGTTGATTTGGGTAGTGCCCCTGGCAGCTGGTCTCAAGTGGCTGGTCACTTGGTTGGTGAAAAA
GGCATCTTGATTGCCTCTGATATTTTGCCGATGGATACCTTGCCGGATGTTACCTTTATCCAAGGTGACTTTCGCGAGCC
AGAAGTGTTTGACCGTATCATGGCTGAGGTTGGTGACAGACAAGTTGATGTGGTGCTGTCTGATATGGCGCCCAATACAG
CAGGTAATAGTGCCATTGATCAGCCGCGTATGATGTACTTATGTGAGCTGGCGGTCGATTTCGCGCTGGCAACCTTACCA
GAAGGTGGCGCGCTTATTATGAAAGTGTTTCAAGGCGAGGGTACACAAGAATTACGCAAGCAGATGCAGGCGGATTTTAG
TAAAATCCGTAGTATTAAGCCTGGTGCTTCTAGACCGCGCTCAAAAGAGATATTTTGGATCGCAATTAAGTAA

Upstream 100 bases:

>100_bases
GTATGGTTGTAGAAAGTGACATAAAGCAAAAAGTCTATTAAGACATTAGTTAATAAATACATATGCGCATATAGAAAAGC
ACGTTATAGGAGCAGGAATT

Downstream 100 bases:

>100_bases
TATGAAATGAAACTCTCACCTCAAAATTAGCCAGTTAAAATCATTGAATGAAAACGGCTACAATCGTTAACTGAATAAAT
GGGAATGCTGCTTGAATTAT

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MATRIENKKLSKSSSAWMKEHIDDHYVQKAQKDGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGHLVGEK
GILIASDILPMDTLPDVTFIQGDFREPEVFDRIMAEVGDRQVDVVLSDMAPNTAGNSAIDQPRMMYLCELAVDFALATLP
EGGALIMKVFQGEGTQELRKQMQADFSKIRSIKPGASRPRSKEIFWIAIK

Sequences:

>Translated_210_residues
MATRIENKKLSKSSSAWMKEHIDDHYVQKAQKDGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGHLVGEK
GILIASDILPMDTLPDVTFIQGDFREPEVFDRIMAEVGDRQVDVVLSDMAPNTAGNSAIDQPRMMYLCELAVDFALATLP
EGGALIMKVFQGEGTQELRKQMQADFSKIRSIKPGASRPRSKEIFWIAIK
>Mature_209_residues
ATRIENKKLSKSSSAWMKEHIDDHYVQKAQKDGYRARAAYKLLEINEKTNLIKKGMTVVDLGSAPGSWSQVAGHLVGEKG
ILIASDILPMDTLPDVTFIQGDFREPEVFDRIMAEVGDRQVDVVLSDMAPNTAGNSAIDQPRMMYLCELAVDFALATLPE
GGALIMKVFQGEGTQELRKQMQADFSKIRSIKPGASRPRSKEIFWIAIK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=219, Percent_Identity=32.8767123287671, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI194097365, Length=185, Percent_Identity=32.972972972973, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI29029591, Length=187, Percent_Identity=34.2245989304813, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI29029589, Length=187, Percent_Identity=34.2245989304813, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI7110661, Length=187, Percent_Identity=34.2245989304813, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=50, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17553474, Length=198, Percent_Identity=33.8383838383838, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=32.4324324324324, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=185, Percent_Identity=32.4324324324324, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=185, Percent_Identity=32.4324324324324, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554650, Length=189, Percent_Identity=31.2169312169312, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=196, Percent_Identity=34.1836734693878, Blast_Score=115, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=186, Percent_Identity=33.8709677419355, Blast_Score=108, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=113, Percent_Identity=34.5132743362832, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356387, Length=210, Percent_Identity=36.6666666666667, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24647580, Length=195, Percent_Identity=32.8205128205128, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859957, Length=185, Percent_Identity=30.8108108108108, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=31.0880829015544, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23329; Mature: 23198

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRIENKKLSKSSSAWMKEHIDDHYVQKAQKDGYRARAAYKLLEINEKTNLIKKGMTVV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCEEE
DLGSAPGSWSQVAGHLVGEKGILIASDILPMDTLPDVTFIQGDFREPEVFDRIMAEVGDR
ECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC
QVDVVLSDMAPNTAGNSAIDQPRMMYLCELAVDFALATLPEGGALIMKVFQGEGTQELRK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
QMQADFSKIRSIKPGASRPRSKEIFWIAIK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
ATRIENKKLSKSSSAWMKEHIDDHYVQKAQKDGYRARAAYKLLEINEKTNLIKKGMTVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCEEE
DLGSAPGSWSQVAGHLVGEKGILIASDILPMDTLPDVTFIQGDFREPEVFDRIMAEVGDR
ECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCC
QVDVVLSDMAPNTAGNSAIDQPRMMYLCELAVDFALATLPEGGALIMKVFQGEGTQELRK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
QMQADFSKIRSIKPGASRPRSKEIFWIAIK
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA