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The map label for this gene is pyrG [H]

Identifier: 93006699

GI number: 93006699

Start: 2315015

End: 2316649

Strand: Reverse

Name: pyrG [H]

Synonym: Pcryo_1875

Alternate gene names: 93006699

Gene position: 2316649-2315015 (Counterclockwise)

Preceding gene: 93006700

Following gene: 93006698

Centisome position: 75.71

GC content: 46.06

Gene sequence:

>1635_bases
ATGACCAAGTTTATATTTGTGACCGGTGGGGTAGTGTCCTCACTAGGAAAAGGTATTACCGCAGCTTCCCTTGCTGCCGT
CTTAGAAGCACGGGGCGTAAACGTGACCATGACCAAGATGGATCCGTATATCAACGTCGATCCAGGCACGATGAGCCCGT
TCCAGCATGGTGAAGTGTTCGTGACCGAAGATGGCGCCGAGACGGATTTGGACTTGGGCTATTATGAGCGTTTTTTACGC
CATTCAAAGATGAGCAAAAGCAATAACTTTACCAGCGGTCGCATCTATCAAAACGTCTTGAATAAAGAGCGCCGTGGCGA
ATATCTCGGTGGTACGGTGCAGGTGATTCCGCATATCACTGATGAGATTAAGAGTAAAATTCTTGCCAGTGGCGAAGGGT
ATGATGTTGCTATCATCGAGATTGGCGGGACAGTGGGTGATATCGAGTCGCTACCCTTTATGGAAGCAGTACGTCAAATG
CAAGTCGAGCTAGGACGCAATCGCGCCATGCTGATGCATCTGACATTGGTGCCTTATATTGCCAGTGCAGGCGAAACCAA
AACCAAGCCCACTCAGCATTCAGTCAAAGAGCTACGTTCTATCGGTTTGCAGCCTGATATCTTGATTTGCCGCTCTGATC
ATCATATCTCACAAGACAATCGCCGCAAGATTGCACTATTTACCAACGTTGAAGAGCGTGCGGTCATTATGTGTGAAGAT
GCGCAAAGTATTTATCAGATTCCACGTACCTTACATGAGCAAGATCTTGATGATTTAATCTGTGAGCGTTTTGGTCTTGA
TTTACCAGAAGCAGATTTGAGCGATTGGGATAAGGTTGTCGAAGCGCAGCTGAATCCTGAGTCGACGGTAACAGTAGCCA
TGGTTGGTAAATATGTTGAATTACCAGATGCCTATAAATCAATCAATGAAGCATTGTTACATGCCGGTATTACTCACAAA
GCCGACGTCAAAATTGATTATATCGATGCTGAACGTTTAGAAGACGATGACAGCTTACTAGCGCAACTGCATAATGCTGA
TGCCATCTTGGTGCCAGGCGGCTTTGGTGAGCGCGGTACGATGGGTAAGATCAAAGCCATTACCTATGCTCGCGAAAACA
ACGTACCTTATTTAGGTATCTGTTTAGGTATGCAGCTTGCAGTGATTGAATATGCGCGTAACGTGCTACATATCGATGCC
AACTCTTCTGAATTTGATCGTAAAACAGCTGAGCCTATTATTGGCTTGATTACAGAGTGGTTAGATGAGCGCGGCGAATT
GCAGATTCGTAGTGATGATTCAGACCTTGGTGGTACGATGCGTTTAGGTGCGCAGCAAGCTGAGCTGGTCGCTGGTAGTA
AGCTTGCACAAATCTACGGTGCTAACAATATAACTGAGCGTCATCGTCATCGCTATGAGATGAATAACCGCTATATCGAG
CCACTAGAGCAGGCAGGAATGACGGTATCAGGTTATTCTGCTAAGCAGCACCTAGTTGAGTCAGTTGAACTTGCTGATCA
TCCATGGTTTGTTGCGGTACAGTTCCATCCAGAATTTACCAGCTCGCCACGCGGTGGTCATCCATTGTTCAATAGCTTTG
TAAAAGCGGCGAAGAATTATAGTGAAGCGAAATAA

Upstream 100 bases:

>100_bases
TTGCTGAAATTTAATAATTTAATAGAGTTTGTGCTTTTGCCCTATATTTTATGGCGTATTTTTAGTAAAATCCTTCTTTA
CCAATTACCGACAGAGTACT

Downstream 100 bases:

>100_bases
TGGTTAAGTTATAAGTGCTTTTGATATAAAAAGACTGATCTTCGGATTGGTCTTTTTTTTTGGCTGATTTTTTGGCAGTG
GTTTGCATGCTGATTTTTCT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTKFIFVTGGVVSSLGKGITAASLAAVLEARGVNVTMTKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
HSKMSKSNNFTSGRIYQNVLNKERRGEYLGGTVQVIPHITDEIKSKILASGEGYDVAIIEIGGTVGDIESLPFMEAVRQM
QVELGRNRAMLMHLTLVPYIASAGETKTKPTQHSVKELRSIGLQPDILICRSDHHISQDNRRKIALFTNVEERAVIMCED
AQSIYQIPRTLHEQDLDDLICERFGLDLPEADLSDWDKVVEAQLNPESTVTVAMVGKYVELPDAYKSINEALLHAGITHK
ADVKIDYIDAERLEDDDSLLAQLHNADAILVPGGFGERGTMGKIKAITYARENNVPYLGICLGMQLAVIEYARNVLHIDA
NSSEFDRKTAEPIIGLITEWLDERGELQIRSDDSDLGGTMRLGAQQAELVAGSKLAQIYGANNITERHRHRYEMNNRYIE
PLEQAGMTVSGYSAKQHLVESVELADHPWFVAVQFHPEFTSSPRGGHPLFNSFVKAAKNYSEAK

Sequences:

>Translated_544_residues
MTKFIFVTGGVVSSLGKGITAASLAAVLEARGVNVTMTKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
HSKMSKSNNFTSGRIYQNVLNKERRGEYLGGTVQVIPHITDEIKSKILASGEGYDVAIIEIGGTVGDIESLPFMEAVRQM
QVELGRNRAMLMHLTLVPYIASAGETKTKPTQHSVKELRSIGLQPDILICRSDHHISQDNRRKIALFTNVEERAVIMCED
AQSIYQIPRTLHEQDLDDLICERFGLDLPEADLSDWDKVVEAQLNPESTVTVAMVGKYVELPDAYKSINEALLHAGITHK
ADVKIDYIDAERLEDDDSLLAQLHNADAILVPGGFGERGTMGKIKAITYARENNVPYLGICLGMQLAVIEYARNVLHIDA
NSSEFDRKTAEPIIGLITEWLDERGELQIRSDDSDLGGTMRLGAQQAELVAGSKLAQIYGANNITERHRHRYEMNNRYIE
PLEQAGMTVSGYSAKQHLVESVELADHPWFVAVQFHPEFTSSPRGGHPLFNSFVKAAKNYSEAK
>Mature_543_residues
TKFIFVTGGVVSSLGKGITAASLAAVLEARGVNVTMTKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLRH
SKMSKSNNFTSGRIYQNVLNKERRGEYLGGTVQVIPHITDEIKSKILASGEGYDVAIIEIGGTVGDIESLPFMEAVRQMQ
VELGRNRAMLMHLTLVPYIASAGETKTKPTQHSVKELRSIGLQPDILICRSDHHISQDNRRKIALFTNVEERAVIMCEDA
QSIYQIPRTLHEQDLDDLICERFGLDLPEADLSDWDKVVEAQLNPESTVTVAMVGKYVELPDAYKSINEALLHAGITHKA
DVKIDYIDAERLEDDDSLLAQLHNADAILVPGGFGERGTMGKIKAITYARENNVPYLGICLGMQLAVIEYARNVLHIDAN
SSEFDRKTAEPIIGLITEWLDERGELQIRSDDSDLGGTMRLGAQQAELVAGSKLAQIYGANNITERHRHRYEMNNRYIEP
LEQAGMTVSGYSAKQHLVESVELADHPWFVAVQFHPEFTSSPRGGHPLFNSFVKAAKNYSEAK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=44.2652329749104, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=44.4642857142857, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=44.4642857142857, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=44.4642857142857, Blast_Score=462, Evalue=1e-130,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=63.8376383763838, Blast_Score=705, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=38.3360522022839, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322563, Length=568, Percent_Identity=41.1971830985916, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=554, Percent_Identity=40.9747292418773, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=42.2939068100358, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=40.9542743538767, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60425; Mature: 60294

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFIFVTGGVVSSLGKGITAASLAAVLEARGVNVTMTKMDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRHSKMSKSNNFTSGRIYQNVLNKERRGEYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHH
DEIKSKILASGEGYDVAIIEIGGTVGDIESLPFMEAVRQMQVELGRNRAMLMHLTLVPYI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
ASAGETKTKPTQHSVKELRSIGLQPDILICRSDHHISQDNRRKIALFTNVEERAVIMCED
HCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECC
AQSIYQIPRTLHEQDLDDLICERFGLDLPEADLSDWDKVVEAQLNPESTVTVAMVGKYVE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHC
LPDAYKSINEALLHAGITHKADVKIDYIDAERLEDDDSLLAQLHNADAILVPGGFGERGT
CCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCCHHHHHHHHCCCCEEEECCCCCCCCC
MGKIKAITYARENNVPYLGICLGMQLAVIEYARNVLHIDANSSEFDRKTAEPIIGLITEW
CCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
LDERGELQIRSDDSDLGGTMRLGAQQAELVAGSKLAQIYGANNITERHRHRYEMNNRYIE
HCCCCCEEEECCCCCCCCEEECCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCC
PLEQAGMTVSGYSAKQHLVESVELADHPWFVAVQFHPEFTSSPRGGHPLFNSFVKAAKNY
HHHHCCCEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
SEAK
CCCC
>Mature Secondary Structure 
TKFIFVTGGVVSSLGKGITAASLAAVLEARGVNVTMTKMDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRHSKMSKSNNFTSGRIYQNVLNKERRGEYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHH
DEIKSKILASGEGYDVAIIEIGGTVGDIESLPFMEAVRQMQVELGRNRAMLMHLTLVPYI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
ASAGETKTKPTQHSVKELRSIGLQPDILICRSDHHISQDNRRKIALFTNVEERAVIMCED
HCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECC
AQSIYQIPRTLHEQDLDDLICERFGLDLPEADLSDWDKVVEAQLNPESTVTVAMVGKYVE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHC
LPDAYKSINEALLHAGITHKADVKIDYIDAERLEDDDSLLAQLHNADAILVPGGFGERGT
CCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCCHHHHHHHHCCCCEEEECCCCCCCCC
MGKIKAITYARENNVPYLGICLGMQLAVIEYARNVLHIDANSSEFDRKTAEPIIGLITEW
CCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHH
LDERGELQIRSDDSDLGGTMRLGAQQAELVAGSKLAQIYGANNITERHRHRYEMNNRYIE
HCCCCCEEEECCCCCCCCEEECCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCC
PLEQAGMTVSGYSAKQHLVESVELADHPWFVAVQFHPEFTSSPRGGHPLFNSFVKAAKNY
HHHHCCCEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
SEAK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA