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The map label for this gene is truC [H]

Identifier: 93006645

GI number: 93006645

Start: 2246902

End: 2247672

Strand: Reverse

Name: truC [H]

Synonym: Pcryo_1821

Alternate gene names: 93006645

Gene position: 2247672-2246902 (Counterclockwise)

Preceding gene: 93006647

Following gene: 93006643

Centisome position: 73.46

GC content: 48.38

Gene sequence:

>771_bases
ATGAATATCGAAAGCGACACCGCCAATCAAATCGCTAATCAAATAGAAATCATCTATGAAGATGAATTTCTGGTGGCGAT
TAATAAAGAGGCTGGGCTGTTGGTGCATCGCAGTTGGCTGGATAAAGGCGAGACACGCTTTGCCATGCAGCTCACCCGTG
ATGCGGTAGGTTGCCATGTATTTCCGGTGCATAGGTTAGACAAGCCTACGTCAGGCGTGTTGTTGTTTGCCAAGAGCTCA
GCCGTGGCGCGCAGCCTTACGGAGGCTTTTACTGAACACAAAGTGACCAAGCAATATTTAGCTGTGGTGCGCGGCTATAT
GTCAGAACAAGGCACCGTTGATTATGCGTTGAGCTTTCAGCCCGATGCTATTGCCGATAAGTTCGCTAATTTGGATAAAC
CGGCTCAAGAGGCGGTAACCCATTGGCAAAGTTTGGCACAAATTGAGTTGCCATTTGCGGTGTCAAAAAAGCATGACACG
AGCCGCTATAGTCTAATGCGCTTAACCCCCGAGACCGGACGTAAGCATCAACTGCGTCGGCATATGAGACATTTATTCCA
TCATATCGTGGGTGATACCAGCCACGGCGATGGAAGGCACAATCGATTTTTTCGTACTCAATATGGTTGCGCGCGCATGC
TACTGCATGCTCAGACTTTAGCGCTCAGCCATCCGGTCACTGGTGAGCCATTGTTACTAAAAGCGGGATTAGACGATCAA
TGGCTGCGAATTATGGAAGAGTTTGCTTGGGTAGAGAGCGCTAAAGTTTAA

Upstream 100 bases:

>100_bases
AATATGGGCAAAATTATTGGAATACCTTTTATACGTGAATTGGTAGCGTTTGCTAAAAAAGATATATAATTATGATATCT
AAAATATAGGCACTGAAAAC

Downstream 100 bases:

>100_bases
GCAGCGTAAAGTTTAGGGTTTATCTTCAAAAGTCATATCAAAACAGTCGACATACAGGTCTTAGTGTAGTTAGCGGGTTT
TAAAATAATAAAGACCAAAA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNIESDTANQIANQIEIIYEDEFLVAINKEAGLLVHRSWLDKGETRFAMQLTRDAVGCHVFPVHRLDKPTSGVLLFAKSS
AVARSLTEAFTEHKVTKQYLAVVRGYMSEQGTVDYALSFQPDAIADKFANLDKPAQEAVTHWQSLAQIELPFAVSKKHDT
SRYSLMRLTPETGRKHQLRRHMRHLFHHIVGDTSHGDGRHNRFFRTQYGCARMLLHAQTLALSHPVTGEPLLLKAGLDDQ
WLRIMEEFAWVESAKV

Sequences:

>Translated_256_residues
MNIESDTANQIANQIEIIYEDEFLVAINKEAGLLVHRSWLDKGETRFAMQLTRDAVGCHVFPVHRLDKPTSGVLLFAKSS
AVARSLTEAFTEHKVTKQYLAVVRGYMSEQGTVDYALSFQPDAIADKFANLDKPAQEAVTHWQSLAQIELPFAVSKKHDT
SRYSLMRLTPETGRKHQLRRHMRHLFHHIVGDTSHGDGRHNRFFRTQYGCARMLLHAQTLALSHPVTGEPLLLKAGLDDQ
WLRIMEEFAWVESAKV
>Mature_256_residues
MNIESDTANQIANQIEIIYEDEFLVAINKEAGLLVHRSWLDKGETRFAMQLTRDAVGCHVFPVHRLDKPTSGVLLFAKSS
AVARSLTEAFTEHKVTKQYLAVVRGYMSEQGTVDYALSFQPDAIADKFANLDKPAQEAVTHWQSLAQIELPFAVSKKHDT
SRYSLMRLTPETGRKHQLRRHMRHLFHHIVGDTSHGDGRHNRFFRTQYGCARMLLHAQTLALSHPVTGEPLLLKAGLDDQ
WLRIMEEFAWVESAKV

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789155, Length=236, Percent_Identity=52.5423728813559, Blast_Score=266, Evalue=9e-73,
Organism=Escherichia coli, GI1788946, Length=231, Percent_Identity=32.034632034632, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787327, Length=235, Percent_Identity=33.1914893617021, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786244, Length=226, Percent_Identity=34.5132743362832, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320168, Length=188, Percent_Identity=28.1914893617021, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324506, Length=187, Percent_Identity=28.8770053475936, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321608, Length=206, Percent_Identity=27.1844660194175, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583449, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583447, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583445, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 29131; Mature: 29131

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIESDTANQIANQIEIIYEDEFLVAINKEAGLLVHRSWLDKGETRFAMQLTRDAVGCHV
CCCCCHHHHHHHHHEEEEEECCEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHCEEE
FPVHRLDKPTSGVLLFAKSSAVARSLTEAFTEHKVTKQYLAVVRGYMSEQGTVDYALSFQ
EEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PDAIADKFANLDKPAQEAVTHWQSLAQIELPFAVSKKHDTSRYSLMRLTPETGRKHQLRR
CHHHHHHHHCCCCHHHHHHHHHHHHHHEECCEEECCCCCCCCEEEEEECCCCCHHHHHHH
HMRHLFHHIVGDTSHGDGRHNRFFRTQYGCARMLLHAQTLALSHPVTGEPLLLKAGLDDQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHH
WLRIMEEFAWVESAKV
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIESDTANQIANQIEIIYEDEFLVAINKEAGLLVHRSWLDKGETRFAMQLTRDAVGCHV
CCCCCHHHHHHHHHEEEEEECCEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHHHHCEEE
FPVHRLDKPTSGVLLFAKSSAVARSLTEAFTEHKVTKQYLAVVRGYMSEQGTVDYALSFQ
EEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PDAIADKFANLDKPAQEAVTHWQSLAQIELPFAVSKKHDTSRYSLMRLTPETGRKHQLRR
CHHHHHHHHCCCCHHHHHHHHHHHHHHEECCEEECCCCCCCCEEEEEECCCCCHHHHHHH
HMRHLFHHIVGDTSHGDGRHNRFFRTQYGCARMLLHAQTLALSHPVTGEPLLLKAGLDDQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHH
WLRIMEEFAWVESAKV
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]