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The map label for this gene is cls [H]

Identifier: 93006556

GI number: 93006556

Start: 2146207

End: 2147874

Strand: Reverse

Name: cls [H]

Synonym: Pcryo_1732

Alternate gene names: 93006556

Gene position: 2147874-2146207 (Counterclockwise)

Preceding gene: 93006558

Following gene: 93006555

Centisome position: 70.19

GC content: 43.71

Gene sequence:

>1668_bases
TTGATTATATTGTGCTATTACCAGTTGGAGGCGAAAACAATAGAAGCTTTATCTACTACTGAGCAGCAAGTGGCAGCATG
GTCTTGGACAGACTTTGCTGGGCTAGGTCTGATATTTCATATTATTCTTATGATTGTGATGACGTTGCGTATTGTCTCGG
TACAGCGCAATATTGGGGTGGCAATTGCATGGATAGCGGTTTTGTTTACTTTGCCCGTATTTGGCTTGATTGCCTATGTG
CTGGTTGGTGAGCCGATGATAGGTCGACGTTATCGCCAGCGAATGGATCAAGCGCGACTACTGATGAATGAGATGGCACA
ACGGGAGCGCTTGGTGTTTGATGAAGGGCAAGAGGTATTGCCGACTCATTACCGTGGGGTCAGTCATATCGGTACGCGCT
GGACAGGGTTTGGGGTATTCTCAAACCATGATATGCAACTGTTGACACACGCCAATATTATTTTTAAACGTTTGATTGAT
GACATCAATGCCGCTCAGCGCAGTATCTTGATGGAGTTTTATATTATTTATCCTAAGGGGCAGGTGCTTGATGTTATAGA
ATCGTTAATGGCAGCCGCTCAACGCGGAGTTGAGTGTCATATTCTGATTGATAGTGTGGGGAGTTTTAGTTTTTTTAACA
GTAAAGCCCATCGAAAATTAGAAAAAGCAGGCGTTTTTGTCCATCAGTCATTGCCAGTAGGGTTGTTTAAAACGTTATTT
AAGCGTTCAGATTTGCGTAATCATCGTAAAATCGTAGTTATTGATGGTTATATCGGTTATATCGGTAGCTTTAATTTGGT
TGATCCTAAGTTTTTTAAGCAGAATAAAGACGTCGGTGAATGGATTGATGTGGCGATACGCAGTGTCAGTGATAATGACA
TTAGCATTGCGACTGCAATGGCAAAAGTGGTGGTGACCGATATCGGGGCGGAAAACAGTGACAATCTAGATGCGCTGCAT
CAACGAGTCAACAGCTACACACGTAAGCTATATGTGCGCAATCCGACGATTAATGACATCAATAGTCGCATCAAAGTATT
GGATGATGGGATAGAAAATGTAGAAACCCCCTGTACTGGTGCGATGTCGACAGTGATTCCGCAGATGCCGGTAGTAAAAG
GCGTGGTCGCGCAGTTGATTCCTTCAGCGCCACAAGTGACCGCTCACGTTATTTATAATACCTTAGTAACCGTTTTACAT
CGTGCTAATCACTGTATTTGTATCACAACGCCGTACTTTGTACCGGATGAAGCTTTGTCAGGCGCGCTAGTGACGGCAGC
CAAGCGCGGGGTCAAAGTTACGCTGATTATCCCTGAAAAGGTTGATTCATTCTTAGTTCAGCACGCCTCGCAAGCGTATT
ACCAAGAGTTGTTAGAAGCGGGTGTTACCATTGCTCTGTTTAAGGGTGGTCTGCTGCATGCCAAAACAGTAGTTATAGAT
GACGATTACTGTATATTTGGCACCGTCAATATCGATATGCGCAGCTTTTATCTGAACATGGAAGTCAGTTTGGCGATTTA
TACGCCTGAAATGGTCGCGCAAGTTGCTGCTTGTCAAGATTCGTATTTACAAAGCTGTCGTTTTTTAACCTTGGATCAGT
GGCAACGGCGTCATAAGGCTGAACGCTTGTTTGATAATGTGATACGCTTATTTAGTCCATTATTGTAG

Upstream 100 bases:

>100_bases
TATGGCTAGATGATGTAACAGCCCATATGACAACTACCTAGTCAACGAATAGTTACCTATTGCGCGGCGGATAAAAGCTA
CTATACTTGATTGGTCGTAT

Downstream 100 bases:

>100_bases
CCTTACATATTTTAAGTTAAGGTAAGCACTATATTTTGCACAACTGCTTTTAGTTCATTGCCTCTATGATTAATTTACTT
TTTTAAAGGATACGCGTTTC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MIILCYYQLEAKTIEALSTTEQQVAAWSWTDFAGLGLIFHIILMIVMTLRIVSVQRNIGVAIAWIAVLFTLPVFGLIAYV
LVGEPMIGRRYRQRMDQARLLMNEMAQRERLVFDEGQEVLPTHYRGVSHIGTRWTGFGVFSNHDMQLLTHANIIFKRLID
DINAAQRSILMEFYIIYPKGQVLDVIESLMAAAQRGVECHILIDSVGSFSFFNSKAHRKLEKAGVFVHQSLPVGLFKTLF
KRSDLRNHRKIVVIDGYIGYIGSFNLVDPKFFKQNKDVGEWIDVAIRSVSDNDISIATAMAKVVVTDIGAENSDNLDALH
QRVNSYTRKLYVRNPTINDINSRIKVLDDGIENVETPCTGAMSTVIPQMPVVKGVVAQLIPSAPQVTAHVIYNTLVTVLH
RANHCICITTPYFVPDEALSGALVTAAKRGVKVTLIIPEKVDSFLVQHASQAYYQELLEAGVTIALFKGGLLHAKTVVID
DDYCIFGTVNIDMRSFYLNMEVSLAIYTPEMVAQVAACQDSYLQSCRFLTLDQWQRRHKAERLFDNVIRLFSPLL

Sequences:

>Translated_555_residues
MIILCYYQLEAKTIEALSTTEQQVAAWSWTDFAGLGLIFHIILMIVMTLRIVSVQRNIGVAIAWIAVLFTLPVFGLIAYV
LVGEPMIGRRYRQRMDQARLLMNEMAQRERLVFDEGQEVLPTHYRGVSHIGTRWTGFGVFSNHDMQLLTHANIIFKRLID
DINAAQRSILMEFYIIYPKGQVLDVIESLMAAAQRGVECHILIDSVGSFSFFNSKAHRKLEKAGVFVHQSLPVGLFKTLF
KRSDLRNHRKIVVIDGYIGYIGSFNLVDPKFFKQNKDVGEWIDVAIRSVSDNDISIATAMAKVVVTDIGAENSDNLDALH
QRVNSYTRKLYVRNPTINDINSRIKVLDDGIENVETPCTGAMSTVIPQMPVVKGVVAQLIPSAPQVTAHVIYNTLVTVLH
RANHCICITTPYFVPDEALSGALVTAAKRGVKVTLIIPEKVDSFLVQHASQAYYQELLEAGVTIALFKGGLLHAKTVVID
DDYCIFGTVNIDMRSFYLNMEVSLAIYTPEMVAQVAACQDSYLQSCRFLTLDQWQRRHKAERLFDNVIRLFSPLL
>Mature_555_residues
MIILCYYQLEAKTIEALSTTEQQVAAWSWTDFAGLGLIFHIILMIVMTLRIVSVQRNIGVAIAWIAVLFTLPVFGLIAYV
LVGEPMIGRRYRQRMDQARLLMNEMAQRERLVFDEGQEVLPTHYRGVSHIGTRWTGFGVFSNHDMQLLTHANIIFKRLID
DINAAQRSILMEFYIIYPKGQVLDVIESLMAAAQRGVECHILIDSVGSFSFFNSKAHRKLEKAGVFVHQSLPVGLFKTLF
KRSDLRNHRKIVVIDGYIGYIGSFNLVDPKFFKQNKDVGEWIDVAIRSVSDNDISIATAMAKVVVTDIGAENSDNLDALH
QRVNSYTRKLYVRNPTINDINSRIKVLDDGIENVETPCTGAMSTVIPQMPVVKGVVAQLIPSAPQVTAHVIYNTLVTVLH
RANHCICITTPYFVPDEALSGALVTAAKRGVKVTLIIPEKVDSFLVQHASQAYYQELLEAGVTIALFKGGLLHAKTVVID
DDYCIFGTVNIDMRSFYLNMEVSLAIYTPEMVAQVAACQDSYLQSCRFLTLDQWQRRHKAERLFDNVIRLFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=528, Percent_Identity=35.2272727272727, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1787007, Length=112, Percent_Identity=31.25, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 62450; Mature: 62450

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILCYYQLEAKTIEALSTTEQQVAAWSWTDFAGLGLIFHIILMIVMTLRIVSVQRNIGV
CEEEEEEEECHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AIAWIAVLFTLPVFGLIAYVLVGEPMIGRRYRQRMDQARLLMNEMAQRERLVFDEGQEVL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PTHYRGVSHIGTRWTGFGVFSNHDMQLLTHANIIFKRLIDDINAAQRSILMEFYIIYPKG
CHHHCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEECCCC
QVLDVIESLMAAAQRGVECHILIDSVGSFSFFNSKAHRKLEKAGVFVHQSLPVGLFKTLF
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
KRSDLRNHRKIVVIDGYIGYIGSFNLVDPKFFKQNKDVGEWIDVAIRSVSDNDISIATAM
HHHHHCCCCEEEEEECHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
AKVVVTDIGAENSDNLDALHQRVNSYTRKLYVRNPTINDINSRIKVLDDGIENVETPCTG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCHHHCCCCCCC
AMSTVIPQMPVVKGVVAQLIPSAPQVTAHVIYNTLVTVLHRANHCICITTPYFVPDEALS
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHC
GALVTAAKRGVKVTLIIPEKVDSFLVQHASQAYYQELLEAGVTIALFKGGLLHAKTVVID
CHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEE
DDYCIFGTVNIDMRSFYLNMEVSLAIYTPEMVAQVAACQDSYLQSCRFLTLDQWQRRHKA
CCEEEEEEEEEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ERLFDNVIRLFSPLL
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIILCYYQLEAKTIEALSTTEQQVAAWSWTDFAGLGLIFHIILMIVMTLRIVSVQRNIGV
CEEEEEEEECHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AIAWIAVLFTLPVFGLIAYVLVGEPMIGRRYRQRMDQARLLMNEMAQRERLVFDEGQEVL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PTHYRGVSHIGTRWTGFGVFSNHDMQLLTHANIIFKRLIDDINAAQRSILMEFYIIYPKG
CHHHCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEEEECCCC
QVLDVIESLMAAAQRGVECHILIDSVGSFSFFNSKAHRKLEKAGVFVHQSLPVGLFKTLF
HHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
KRSDLRNHRKIVVIDGYIGYIGSFNLVDPKFFKQNKDVGEWIDVAIRSVSDNDISIATAM
HHHHHCCCCEEEEEECHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHH
AKVVVTDIGAENSDNLDALHQRVNSYTRKLYVRNPTINDINSRIKVLDDGIENVETPCTG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCHHHCCCCCCC
AMSTVIPQMPVVKGVVAQLIPSAPQVTAHVIYNTLVTVLHRANHCICITTPYFVPDEALS
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHC
GALVTAAKRGVKVTLIIPEKVDSFLVQHASQAYYQELLEAGVTIALFKGGLLHAKTVVID
CHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEE
DDYCIFGTVNIDMRSFYLNMEVSLAIYTPEMVAQVAACQDSYLQSCRFLTLDQWQRRHKA
CCEEEEEEEEEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ERLFDNVIRLFSPLL
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA