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The map label for this gene is rimO

Identifier: 93006539

GI number: 93006539

Start: 2122274

End: 2123869

Strand: Reverse

Name: rimO

Synonym: Pcryo_1715

Alternate gene names: 93006539

Gene position: 2123869-2122274 (Counterclockwise)

Preceding gene: 93006542

Following gene: 93006538

Centisome position: 69.41

GC content: 44.67

Gene sequence:

>1596_bases
ATGCCCAATATTTCTACCGAGTCCGTTAATACGACTATAGCCCCTTCACAGCCAGCTTCTATACCAAAAGACACTGCCAC
TCTCTTTAATCCTGCTAAGCCGACAGCAATGCCTGCGCAAAGCTCGACGGACAGTGTTCAACCTTACCATCATAAAGCCA
ATCATAATCAAAATCGTAGTGTCGAACAAAGCAGCGAGGTAGTTTCAGCGGCTTCAGCTAAAACGACAACAGCAACGACC
AATGCCCCTGTCAATGCCGCGCCAAAGATTGGATTTGTGTCTCTAGGTTGTCCAAAAGCCTTGGTCGATAGTGAGCGCAT
TATCACAGAGCTTAGCCGTGATGGCTATCAAGTAGCCAGTGATTATGAAGGCGCCGACTTGGTAGTGGTCAATACGTGCG
GCTTTATCGAGTCAGCGGTACAAGAGTCGCTCGATGCAATCGGTGAAGCTATCAGCAAAAACGGTAAGGTGATTGTGACA
GGCTGCTTGGGTAAAGAGGCAGATAAAATCCGTGAAATGCATCCTGCTGTACTAGCAGTGACGGGCGCTCATGCTTATGA
TGACGTCATTAGAGCAGTCGCGTTGCATGTACCAAAGCCTGACTGTGGTTTGGATGCCAGTTATGACCCAAAAATAGATT
TGATTAATGAGGCAGGTATCAAATTAACGCCTAGTCATTATGCTTATCTAAAAATATCAGAAGGTTGCAACCACCGTTGT
ACTTTTTGCATTATTCCAAGCTTACGTGGAGACTTGGTTTCACGTCCGATTGACAGTGTGATGAATGAAGCGCTGGCGCT
GAAAAAGGCTGGCGTGAAAGAGTTATTGATTATCTCACAAGATACTTCTGCTTATGGTCTGGACTTAAAGTATAAGACAA
GCTTTTGGAATGGTATGCCGCTGAAGTCTAAATTTTATGACTTATGCCAAGCGTTAAACGATTTGGGTATTTGGGTGCGT
CTACACTATGTGTATCCGTATCCGCATGTAGATAAAGTCGTAGAATTAATGGGTGAGAAGAAATTATTGCCTTATCTTGA
TATTCCATTCCAACATGCCAGCCATCGCATTTTAAAAGCAATGAAGCGTCCTGCTCATAGCGAAAATACCTTGGCGCGTA
TCCATGCATGGCGTGAGATTTGTCCTGATATCGTTATTCGCTCAACCTTCGTTGTTGGCTTCCCTGGCGAGACAGAAGAA
GATTTCCAATGCTTGCTTGATTGGTTAGTAGAAGCCCGTCTAGATCGCGTTGGTGCCTTTACCTATTCAGAAGTTGAAGG
TGCGGTTGCCAATGACTTGCCAAATCATGTGCCAGAAGACGTCAAGCAAGAACGTTACGAGCGTTTGATGACGTTACAGC
AAGATATTTCAGCCCAAAAACTGCAAGAGAAAATTGGCAAAACCTTGATGGTATTGGTCGACGAAATTGATAGAGAAGAA
GGTGTTGCGATCTGCCGTAGTTATGCTGACGCACCAGAGATTGACGGTCACGTCTACGTCGATGATATTGACGCTCATGT
CAAAGTTGGGCAGTTCTTAACGGTTACTATCGACGATGCTAGTGAATATGATTTGTTTGCCAGTTACAAAGGCTAA

Upstream 100 bases:

>100_bases
TATTTAACCATAAATCAACACTATAGAGCGGCAAAACTGGCGATTTTTGTAAAAAAAACGTACAATGCGCACAGCCAATT
TATCTCTAGCAAGGTTAGCC

Downstream 100 bases:

>100_bases
GCAGTCACTGTAATAGGCGCATGAAAATTGCCCAATCGTGGGCAGTTTTTGCTACAATTAGCGCAATTTGACCTTTGTAC
CTGCTTATCCTTAAGTTGGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MPNISTESVNTTIAPSQPASIPKDTATLFNPAKPTAMPAQSSTDSVQPYHHKANHNQNRSVEQSSEVVSAASAKTTTATT
NAPVNAAPKIGFVSLGCPKALVDSERIITELSRDGYQVASDYEGADLVVVNTCGFIESAVQESLDAIGEAISKNGKVIVT
GCLGKEADKIREMHPAVLAVTGAHAYDDVIRAVALHVPKPDCGLDASYDPKIDLINEAGIKLTPSHYAYLKISEGCNHRC
TFCIIPSLRGDLVSRPIDSVMNEALALKKAGVKELLIISQDTSAYGLDLKYKTSFWNGMPLKSKFYDLCQALNDLGIWVR
LHYVYPYPHVDKVVELMGEKKLLPYLDIPFQHASHRILKAMKRPAHSENTLARIHAWREICPDIVIRSTFVVGFPGETEE
DFQCLLDWLVEARLDRVGAFTYSEVEGAVANDLPNHVPEDVKQERYERLMTLQQDISAQKLQEKIGKTLMVLVDEIDREE
GVAICRSYADAPEIDGHVYVDDIDAHVKVGQFLTVTIDDASEYDLFASYKG

Sequences:

>Translated_531_residues
MPNISTESVNTTIAPSQPASIPKDTATLFNPAKPTAMPAQSSTDSVQPYHHKANHNQNRSVEQSSEVVSAASAKTTTATT
NAPVNAAPKIGFVSLGCPKALVDSERIITELSRDGYQVASDYEGADLVVVNTCGFIESAVQESLDAIGEAISKNGKVIVT
GCLGKEADKIREMHPAVLAVTGAHAYDDVIRAVALHVPKPDCGLDASYDPKIDLINEAGIKLTPSHYAYLKISEGCNHRC
TFCIIPSLRGDLVSRPIDSVMNEALALKKAGVKELLIISQDTSAYGLDLKYKTSFWNGMPLKSKFYDLCQALNDLGIWVR
LHYVYPYPHVDKVVELMGEKKLLPYLDIPFQHASHRILKAMKRPAHSENTLARIHAWREICPDIVIRSTFVVGFPGETEE
DFQCLLDWLVEARLDRVGAFTYSEVEGAVANDLPNHVPEDVKQERYERLMTLQQDISAQKLQEKIGKTLMVLVDEIDREE
GVAICRSYADAPEIDGHVYVDDIDAHVKVGQFLTVTIDDASEYDLFASYKG
>Mature_530_residues
PNISTESVNTTIAPSQPASIPKDTATLFNPAKPTAMPAQSSTDSVQPYHHKANHNQNRSVEQSSEVVSAASAKTTTATTN
APVNAAPKIGFVSLGCPKALVDSERIITELSRDGYQVASDYEGADLVVVNTCGFIESAVQESLDAIGEAISKNGKVIVTG
CLGKEADKIREMHPAVLAVTGAHAYDDVIRAVALHVPKPDCGLDASYDPKIDLINEAGIKLTPSHYAYLKISEGCNHRCT
FCIIPSLRGDLVSRPIDSVMNEALALKKAGVKELLIISQDTSAYGLDLKYKTSFWNGMPLKSKFYDLCQALNDLGIWVRL
HYVYPYPHVDKVVELMGEKKLLPYLDIPFQHASHRILKAMKRPAHSENTLARIHAWREICPDIVIRSTFVVGFPGETEED
FQCLLDWLVEARLDRVGAFTYSEVEGAVANDLPNHVPEDVKQERYERLMTLQQDISAQKLQEKIGKTLMVLVDEIDREEG
VAICRSYADAPEIDGHVYVDDIDAHVKVGQFLTVTIDDASEYDLFASYKG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=25.8333333333333, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=25.8333333333333, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI93277076, Length=399, Percent_Identity=26.8170426065163, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=64.7191011235955, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1786882, Length=409, Percent_Identity=29.5843520782396, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=26.0593220338983, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996771, Length=266, Percent_Identity=29.3233082706767, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=472, Percent_Identity=24.7881355932203, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922432, Length=402, Percent_Identity=25.1243781094527, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PSYCK (Q1QA11)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580976.1
- ProteinModelPortal:   Q1QA11
- SMR:   Q1QA11
- STRING:   Q1QA11
- GeneID:   4034595
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1715
- NMPDR:   fig|335284.3.peg.1974
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q1QA11
- ProtClustDB:   PRK14862
- BioCyc:   PCRY335284:PCRYO_1715-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 58415; Mature: 58284

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNISTESVNTTIAPSQPASIPKDTATLFNPAKPTAMPAQSSTDSVQPYHHKANHNQNRS
CCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
VEQSSEVVSAASAKTTTATTNAPVNAAPKIGFVSLGCPKALVDSERIITELSRDGYQVAS
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECC
DYEGADLVVVNTCGFIESAVQESLDAIGEAISKNGKVIVTGCLGKEADKIREMHPAVLAV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEE
TGAHAYDDVIRAVALHVPKPDCGLDASYDPKIDLINEAGIKLTPSHYAYLKISEGCNHRC
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEECCCCEEEEEECCCCCCCE
TFCIIPSLRGDLVSRPIDSVMNEALALKKAGVKELLIISQDTSAYGLDLKYKTSFWNGMP
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEECCCCCCCC
LKSKFYDLCQALNDLGIWVRLHYVYPYPHVDKVVELMGEKKLLPYLDIPFQHASHRILKA
CHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
MKRPAHSENTLARIHAWREICPDIVIRSTFVVGFPGETEEDFQCLLDWLVEARLDRVGAF
HHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
TYSEVEGAVANDLPNHVPEDVKQERYERLMTLQQDISAQKLQEKIGKTLMVLVDEIDREE
CHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GVAICRSYADAPEIDGHVYVDDIDAHVKVGQFLTVTIDDASEYDLFASYKG
HHHHHHHHCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCCCEEEECCC
>Mature Secondary Structure 
PNISTESVNTTIAPSQPASIPKDTATLFNPAKPTAMPAQSSTDSVQPYHHKANHNQNRS
CCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
VEQSSEVVSAASAKTTTATTNAPVNAAPKIGFVSLGCPKALVDSERIITELSRDGYQVAS
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECC
DYEGADLVVVNTCGFIESAVQESLDAIGEAISKNGKVIVTGCLGKEADKIREMHPAVLAV
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEE
TGAHAYDDVIRAVALHVPKPDCGLDASYDPKIDLINEAGIKLTPSHYAYLKISEGCNHRC
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEECCCCEEEEEECCCCCCCE
TFCIIPSLRGDLVSRPIDSVMNEALALKKAGVKELLIISQDTSAYGLDLKYKTSFWNGMP
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEECCCCCCCC
LKSKFYDLCQALNDLGIWVRLHYVYPYPHVDKVVELMGEKKLLPYLDIPFQHASHRILKA
CHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
MKRPAHSENTLARIHAWREICPDIVIRSTFVVGFPGETEEDFQCLLDWLVEARLDRVGAF
HHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
TYSEVEGAVANDLPNHVPEDVKQERYERLMTLQQDISAQKLQEKIGKTLMVLVDEIDREE
CHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GVAICRSYADAPEIDGHVYVDDIDAHVKVGQFLTVTIDDASEYDLFASYKG
HHHHHHHHCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA