Click here to switch to the map view.

The map label for this gene is ubiB [C]

Identifier: 93006303

GI number: 93006303

Start: 1816814

End: 1818115

Strand: Direct

Name: ubiB [C]

Synonym: Pcryo_1477

Alternate gene names: 93006303

Gene position: 1816814-1818115 (Clockwise)

Preceding gene: 93006302

Following gene: 93006305

Centisome position: 59.38

GC content: 44.32

Gene sequence:

>1302_bases
ATGGCAAAATCGTCCGGAACGCGCTTTATGAAACTTGCTGGCATGACAGCAAGTATTGCTGGAAAAGCAGCTAAAAACTC
CTTAAAGCATCTCTCAAGTGACGAAGAAAAACGCCTACAAGCGCGCTCAGAATTGATGCAAGATGTCGGTATTCAAATCG
CTGAAACTTTAGGTGAGATGAAAGGTGCGGTCATGAAGGTAGGTCAAATTGCCTCGCAGTATAAAGATGTGTTTCCACCT
GAAGTTGCAACCGCATTAGAGAAACTGCAAAAAGATGCACCGGCGATGCCCTATGCGCAGATACGCACACAAGTTGAAAG
TGAGCTCAAAGCACCTATTCATGAATTGTTTACTGAATTCGAAGAAATGCCATTTGCTGCAGCTTCCATCGGACAGGTAC
ATCGCGCAATTTTGCCATCTGGGCAAAGGGTTGTGGTTAAGGTGCAATACCCTGATGTCGACAAAAATTGTGATAGCGAC
CTAAAACAAGTGCGTATGGCACTAAAAATCGCTGGCGTGCTCAATATGAGTAAGCAATTGCAAGAGCAGTTATTTCACGA
AATTCGCCAAAGCTTACATGATGAGCTTGATTATATTAAAGAGGCGCATAATCTGCGGGTATTCGGCGCTTTTCATGCCG
AAGATACGGGTCTCATCATCCCAAAAGTGATTAGCAGTCACTCTTCTAAAAGAATCTTGACCTTGACAGAAGAGAGCGGC
GAAACCTTAACAGTGGCAGCAACGTGGGATAATGATATTAAGCAAAAAATCGCAGTGCGCTTGTTTCATTTTACCGCAGG
GCAGCTGTTTGGGTTATATCGAATGCACTGCGACCCTCATCCGGGCAACTTTGCGTTCCGTGCTGATGGCAGTGTAGTTG
CCTATGATTTTGGCGGGATTCGTAGCTATAGCGACAGCGAAGTTCAATTATTTCGCCGATTTGCCAAGCATGCCTTAAAA
GGTGATGTCACTGCCCTTGAGCAAGATTTGGTTGCTCTGGATATACGCCGTGAAGACGATAAAATCGTACCGGGCGAGTT
TTATGGAAAATGGTTAGAGATTGGGCTGAAACCCTTATCTATCAAACCCTATCAAGACGGTAATTTTGATTTTGCAAGCA
GTCAAACCCATCATGAAGCCATCGCCCAAATGCGCACTTCTTTGAAATACTTTGGACAGTTTCAACCATCAGCCTCTACG
ATGATGCTCGATCGTACTATATCAGGACAATACTGGAACCTAGTCAATCTAGGAGTGACGATAGATTTGTCGCCACTGGT
ACATGAATATATAGGTTTTTAG

Upstream 100 bases:

>100_bases
TACTTTTAGCACAGTAGTTAAACAGCATTCATTCGATTAAAATAATAATATTAGGGGTTAAATAATAACCCACTAAATAC
CCCACTATATGGTGATAGGC

Downstream 100 bases:

>100_bases
AGCGTTAAATGGCTATAAATATATAGGACAAGTTTTAGTATTGATTATACATGCAAGGCATGTCCACAGCGGTCACAAAA
ATCCGCACCTACTGCGTGAC

Product: hypothetical protein

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAKSSGTRFMKLAGMTASIAGKAAKNSLKHLSSDEEKRLQARSELMQDVGIQIAETLGEMKGAVMKVGQIASQYKDVFPP
EVATALEKLQKDAPAMPYAQIRTQVESELKAPIHELFTEFEEMPFAAASIGQVHRAILPSGQRVVVKVQYPDVDKNCDSD
LKQVRMALKIAGVLNMSKQLQEQLFHEIRQSLHDELDYIKEAHNLRVFGAFHAEDTGLIIPKVISSHSSKRILTLTEESG
ETLTVAATWDNDIKQKIAVRLFHFTAGQLFGLYRMHCDPHPGNFAFRADGSVVAYDFGGIRSYSDSEVQLFRRFAKHALK
GDVTALEQDLVALDIRREDDKIVPGEFYGKWLEIGLKPLSIKPYQDGNFDFASSQTHHEAIAQMRTSLKYFGQFQPSAST
MMLDRTISGQYWNLVNLGVTIDLSPLVHEYIGF

Sequences:

>Translated_433_residues
MAKSSGTRFMKLAGMTASIAGKAAKNSLKHLSSDEEKRLQARSELMQDVGIQIAETLGEMKGAVMKVGQIASQYKDVFPP
EVATALEKLQKDAPAMPYAQIRTQVESELKAPIHELFTEFEEMPFAAASIGQVHRAILPSGQRVVVKVQYPDVDKNCDSD
LKQVRMALKIAGVLNMSKQLQEQLFHEIRQSLHDELDYIKEAHNLRVFGAFHAEDTGLIIPKVISSHSSKRILTLTEESG
ETLTVAATWDNDIKQKIAVRLFHFTAGQLFGLYRMHCDPHPGNFAFRADGSVVAYDFGGIRSYSDSEVQLFRRFAKHALK
GDVTALEQDLVALDIRREDDKIVPGEFYGKWLEIGLKPLSIKPYQDGNFDFASSQTHHEAIAQMRTSLKYFGQFQPSAST
MMLDRTISGQYWNLVNLGVTIDLSPLVHEYIGF
>Mature_432_residues
AKSSGTRFMKLAGMTASIAGKAAKNSLKHLSSDEEKRLQARSELMQDVGIQIAETLGEMKGAVMKVGQIASQYKDVFPPE
VATALEKLQKDAPAMPYAQIRTQVESELKAPIHELFTEFEEMPFAAASIGQVHRAILPSGQRVVVKVQYPDVDKNCDSDL
KQVRMALKIAGVLNMSKQLQEQLFHEIRQSLHDELDYIKEAHNLRVFGAFHAEDTGLIIPKVISSHSSKRILTLTEESGE
TLTVAATWDNDIKQKIAVRLFHFTAGQLFGLYRMHCDPHPGNFAFRADGSVVAYDFGGIRSYSDSEVQLFRRFAKHALKG
DVTALEQDLVALDIRREDDKIVPGEFYGKWLEIGLKPLSIKPYQDGNFDFASSQTHHEAIAQMRTSLKYFGQFQPSASTM
MLDRTISGQYWNLVNLGVTIDLSPLVHEYIGF

Specific function: Required, Probably Indirectly, For The Hydroxylation Of 2-Octaprenylphenol To 2-Octaprenyl-6-Hydroxy-Phenol, The Fourth Step In Ubiquinone Biosynthesis. Specific For Aerobically Grown Log-Phase Cells. [C]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. ADCK protein kinase family [H]

Homologues:

Organism=Homo sapiens, GI34147522, Length=331, Percent_Identity=30.5135951661631, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI27363457, Length=383, Percent_Identity=27.4151436031332, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI217416386, Length=375, Percent_Identity=26.4, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI40254938, Length=283, Percent_Identity=27.208480565371, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI41393593, Length=235, Percent_Identity=31.063829787234, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI217035081, Length=188, Percent_Identity=28.1914893617021, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367309, Length=241, Percent_Identity=30.7053941908714, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32565180, Length=277, Percent_Identity=30.6859205776173, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17559152, Length=346, Percent_Identity=30.0578034682081, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321319, Length=280, Percent_Identity=31.4285714285714, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323282, Length=310, Percent_Identity=27.0967741935484, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859849, Length=399, Percent_Identity=25.8145363408521, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI22024280, Length=295, Percent_Identity=27.7966101694915, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24662575, Length=287, Percent_Identity=28.2229965156794, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 48440; Mature: 48309

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSSGTRFMKLAGMTASIAGKAAKNSLKHLSSDEEKRLQARSELMQDVGIQIAETLGEM
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGAVMKVGQIASQYKDVFPPEVATALEKLQKDAPAMPYAQIRTQVESELKAPIHELFTEF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEMPFAAASIGQVHRAILPSGQRVVVKVQYPDVDKNCDSDLKQVRMALKIAGVLNMSKQL
HHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEQLFHEIRQSLHDELDYIKEAHNLRVFGAFHAEDTGLIIPKVISSHSSKRILTLTEESG
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEHHHHHCCCCCCEEEEEEECCC
ETLTVAATWDNDIKQKIAVRLFHFTAGQLFGLYRMHCDPHPGNFAFRADGSVVAYDFGGI
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCC
RSYSDSEVQLFRRFAKHALKGDVTALEQDLVALDIRREDDKIVPGEFYGKWLEIGLKPLS
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCE
IKPYQDGNFDFASSQTHHEAIAQMRTSLKYFGQFQPSASTMMLDRTISGQYWNLVNLGVT
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECEE
IDLSPLVHEYIGF
EECHHHHHHHHCC
>Mature Secondary Structure 
AKSSGTRFMKLAGMTASIAGKAAKNSLKHLSSDEEKRLQARSELMQDVGIQIAETLGEM
CCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KGAVMKVGQIASQYKDVFPPEVATALEKLQKDAPAMPYAQIRTQVESELKAPIHELFTEF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEMPFAAASIGQVHRAILPSGQRVVVKVQYPDVDKNCDSDLKQVRMALKIAGVLNMSKQL
HHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEQLFHEIRQSLHDELDYIKEAHNLRVFGAFHAEDTGLIIPKVISSHSSKRILTLTEESG
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEHHHHHCCCCCCEEEEEEECCC
ETLTVAATWDNDIKQKIAVRLFHFTAGQLFGLYRMHCDPHPGNFAFRADGSVVAYDFGGI
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCC
RSYSDSEVQLFRRFAKHALKGDVTALEQDLVALDIRREDDKIVPGEFYGKWLEIGLKPLS
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCE
IKPYQDGNFDFASSQTHHEAIAQMRTSLKYFGQFQPSASTMMLDRTISGQYWNLVNLGVT
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECEE
IDLSPLVHEYIGF
EECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]