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The map label for this gene is sbcD [H]

Identifier: 93006224

GI number: 93006224

Start: 1709496

End: 1711049

Strand: Direct

Name: sbcD [H]

Synonym: Pcryo_1398

Alternate gene names: 93006224

Gene position: 1709496-1711049 (Clockwise)

Preceding gene: 93006223

Following gene: 93006225

Centisome position: 55.87

GC content: 44.47

Gene sequence:

>1554_bases
ATGCCTGTATCTGCTGTTACGCCTCTGACTATCCTGCACACCTCTGACTGGCATTTGGGACGCAGGCTTTATGGACGTCT
GCGCTACGATGAATTTTCTGCATTTTTAAGCTGGCTACAAGACACCATTAGTGCGCAGCAAGTGGATGTACTTATTGTCG
CTGGTGATATCTTTGATACCATGACGCCAAGCAATAAAGCCCAAGCGCTGTATTACGAGTTTTTAGGCAAGGTTTCAAAG
TCATGCTGTCAACATATCGTCATCGTCGCTGGCAACCATGATTCACCGACATTTCTAGATGCCCCAAGCAATGTCCTTAA
GTTTTTAAATGTCCATGTCGTTGGTACCGCTTGTGATGACTTAAATGACGAAGTGATGGTGTTAGATGATGTAGATGGCA
CACCGCATTGCATTATTGCCGCTGTCCCTTATCTGCGTGACCGCGACGTGCGTAACAGTAGTGCTGGCGAGTCAGCTAAA
AGCAAAGACGCCAATGTCATCGCTGGTATCTGCGCGCACTATGATGAAGTCGCAGAGATTGCCAACGCCAAACAAGCTGA
ATTATTAAAATCGCATAAGCGCCATATCCCTATTATTGCCACAGGGCATCTGTTCGCTGCCGGCGGCACCACTACTGATG
ATGATGGTGTTCGTGAGCTATATGTTGGTTCACTTGGTAAAGTCTCCGCTGATATGTTCAGTGAAAGCTTTGATTACGTG
GCGCTTGGGCATTTACATGTGCCGCAGCGCGTCGGTGGGCGTGAAACCATTCGATATTCAGGATCACCCATTGCAATGGG
CTTTGGTGAGGCGCGCCAGCAAAAGCAAGTATTGCTCATACAGTTTGGCACGGTGCTAAAAAACAAAGAGCTAACACTGG
AAAATACCTCTGAGAGCAGCCCTCATCAGCTGAACAACACCCTTATTAACACTGATAAGACCGTAGAAAAAACCGTTAAA
AAAGCAGTCAATCAGGCGGCAAGTTTTATGGATGACCTATTTGCTGATGTCGAGTCTCTAGAAAGTGATCAGCATAGTGA
AACGAGTATTGAAGAACCCTCTGAACAAACAGAAGTTATTCTAAGCACTCCTTTCACTTTACCCGCCACGCCACTGCATC
ATGATACTTCACGTCAGATGCAAGTTATCTCAATGCCTATTCCAAAGTTCCAAAACTTGGCGCAAGTATCAGGTGATTTG
GAGACTATTAATCAAAAAGTTGATAATGTTATTGAAGCTCTGTCTGATACAGAATCGGTCTGGTTAGAGATTATCTACAC
TGGTGATGAGGTGATTAGCGAATTGCGTGAAAGCATTCAAGCGATGGTAGAAGGCTTGCCGTGTGAGGTGTTAAAAATAA
AAAATACGCGCACTTATAACAAAGTACTTAATCAGCAACAAATCTCTGAGACGCTACAAGACTTAAATGAACATGAAGTA
TTTGAGCGTTGTCTAGCCACTCATAACATCCCCGATGAGCAAAGATTAACCTTGCGAGAGGCTTATGAGCAAATCGTTTA
TGACCTACATCATGCAGATACTCAAGCTGAATAA

Upstream 100 bases:

>100_bases
CCAATCTAGTGGTAGGGCTTTTTTCTGATTGCAACAGAGCAAACTTCTCTAATCCTTGCTAGAATAATGGCTGTCTTTTT
ATTCACCACTAAGCCTCATT

Downstream 100 bases:

>100_bases
TAGCTAAAACTCATCAGCCTGATACAATTAACCGCAAATTATCATTGATATCTATAAAAATAAGACCCTGCCATGCGCCT
AATTGAACTCAGACTTAAAA

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MPVSAVTPLTILHTSDWHLGRRLYGRLRYDEFSAFLSWLQDTISAQQVDVLIVAGDIFDTMTPSNKAQALYYEFLGKVSK
SCCQHIVIVAGNHDSPTFLDAPSNVLKFLNVHVVGTACDDLNDEVMVLDDVDGTPHCIIAAVPYLRDRDVRNSSAGESAK
SKDANVIAGICAHYDEVAEIANAKQAELLKSHKRHIPIIATGHLFAAGGTTTDDDGVRELYVGSLGKVSADMFSESFDYV
ALGHLHVPQRVGGRETIRYSGSPIAMGFGEARQQKQVLLIQFGTVLKNKELTLENTSESSPHQLNNTLINTDKTVEKTVK
KAVNQAASFMDDLFADVESLESDQHSETSIEEPSEQTEVILSTPFTLPATPLHHDTSRQMQVISMPIPKFQNLAQVSGDL
ETINQKVDNVIEALSDTESVWLEIIYTGDEVISELRESIQAMVEGLPCEVLKIKNTRTYNKVLNQQQISETLQDLNEHEV
FERCLATHNIPDEQRLTLREAYEQIVYDLHHADTQAE

Sequences:

>Translated_517_residues
MPVSAVTPLTILHTSDWHLGRRLYGRLRYDEFSAFLSWLQDTISAQQVDVLIVAGDIFDTMTPSNKAQALYYEFLGKVSK
SCCQHIVIVAGNHDSPTFLDAPSNVLKFLNVHVVGTACDDLNDEVMVLDDVDGTPHCIIAAVPYLRDRDVRNSSAGESAK
SKDANVIAGICAHYDEVAEIANAKQAELLKSHKRHIPIIATGHLFAAGGTTTDDDGVRELYVGSLGKVSADMFSESFDYV
ALGHLHVPQRVGGRETIRYSGSPIAMGFGEARQQKQVLLIQFGTVLKNKELTLENTSESSPHQLNNTLINTDKTVEKTVK
KAVNQAASFMDDLFADVESLESDQHSETSIEEPSEQTEVILSTPFTLPATPLHHDTSRQMQVISMPIPKFQNLAQVSGDL
ETINQKVDNVIEALSDTESVWLEIIYTGDEVISELRESIQAMVEGLPCEVLKIKNTRTYNKVLNQQQISETLQDLNEHEV
FERCLATHNIPDEQRLTLREAYEQIVYDLHHADTQAE
>Mature_516_residues
PVSAVTPLTILHTSDWHLGRRLYGRLRYDEFSAFLSWLQDTISAQQVDVLIVAGDIFDTMTPSNKAQALYYEFLGKVSKS
CCQHIVIVAGNHDSPTFLDAPSNVLKFLNVHVVGTACDDLNDEVMVLDDVDGTPHCIIAAVPYLRDRDVRNSSAGESAKS
KDANVIAGICAHYDEVAEIANAKQAELLKSHKRHIPIIATGHLFAAGGTTTDDDGVRELYVGSLGKVSADMFSESFDYVA
LGHLHVPQRVGGRETIRYSGSPIAMGFGEARQQKQVLLIQFGTVLKNKELTLENTSESSPHQLNNTLINTDKTVEKTVKK
AVNQAASFMDDLFADVESLESDQHSETSIEEPSEQTEVILSTPFTLPATPLHHDTSRQMQVISMPIPKFQNLAQVSGDLE
TINQKVDNVIEALSDTESVWLEIIYTGDEVISELRESIQAMVEGLPCEVLKIKNTRTYNKVLNQQQISETLQDLNEHEVF
ERCLATHNIPDEQRLTLREAYEQIVYDLHHADTQAE

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=274, Percent_Identity=39.7810218978102, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 57386; Mature: 57255

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSAVTPLTILHTSDWHLGRRLYGRLRYDEFSAFLSWLQDTISAQQVDVLIVAGDIFDT
CCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHEEEEEEECCHHHC
MTPSNKAQALYYEFLGKVSKSCCQHIVIVAGNHDSPTFLDAPSNVLKFLNVHVVGTACDD
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEECCHHHHHHHHHHEEEEEECCC
LNDEVMVLDDVDGTPHCIIAAVPYLRDRDVRNSSAGESAKSKDANVIAGICAHYDEVAEI
CCCCEEEEECCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ANAKQAELLKSHKRHIPIIATGHLFAAGGTTTDDDGVRELYVGSLGKVSADMFSESFDYV
HCCHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCEE
ALGHLHVPQRVGGRETIRYSGSPIAMGFGEARQQKQVLLIQFGTVLKNKELTLENTSESS
EEECEECCHHCCCCCEEEECCCEEEECCCHHHHHHCEEEEEHHHHHCCCCEEECCCCCCC
PHQLNNTLINTDKTVEKTVKKAVNQAASFMDDLFADVESLESDQHSETSIEEPSEQTEVI
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LSTPFTLPATPLHHDTSRQMQVISMPIPKFQNLAQVSGDLETINQKVDNVIEALSDTESV
EECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCE
WLEIIYTGDEVISELRESIQAMVEGLPCEVLKIKNTRTYNKVLNQQQISETLQDLNEHEV
EEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHH
FERCLATHNIPDEQRLTLREAYEQIVYDLHHADTQAE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PVSAVTPLTILHTSDWHLGRRLYGRLRYDEFSAFLSWLQDTISAQQVDVLIVAGDIFDT
CCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHEEEEEEECCHHHC
MTPSNKAQALYYEFLGKVSKSCCQHIVIVAGNHDSPTFLDAPSNVLKFLNVHVVGTACDD
CCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEECCHHHHHHHHHHEEEEEECCC
LNDEVMVLDDVDGTPHCIIAAVPYLRDRDVRNSSAGESAKSKDANVIAGICAHYDEVAEI
CCCCEEEEECCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ANAKQAELLKSHKRHIPIIATGHLFAAGGTTTDDDGVRELYVGSLGKVSADMFSESFDYV
HCCHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCEE
ALGHLHVPQRVGGRETIRYSGSPIAMGFGEARQQKQVLLIQFGTVLKNKELTLENTSESS
EEECEECCHHCCCCCEEEECCCEEEECCCHHHHHHCEEEEEHHHHHCCCCEEECCCCCCC
PHQLNNTLINTDKTVEKTVKKAVNQAASFMDDLFADVESLESDQHSETSIEEPSEQTEVI
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LSTPFTLPATPLHHDTSRQMQVISMPIPKFQNLAQVSGDLETINQKVDNVIEALSDTESV
EECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCE
WLEIIYTGDEVISELRESIQAMVEGLPCEVLKIKNTRTYNKVLNQQQISETLQDLNEHEV
EEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHH
FERCLATHNIPDEQRLTLREAYEQIVYDLHHADTQAE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]