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The map label for this gene is cysG [H]

Identifier: 93006223

GI number: 93006223

Start: 1707772

End: 1709358

Strand: Direct

Name: cysG [H]

Synonym: Pcryo_1397

Alternate gene names: 93006223

Gene position: 1707772-1709358 (Clockwise)

Preceding gene: 93006222

Following gene: 93006224

Centisome position: 55.81

GC content: 45.49

Gene sequence:

>1587_bases
ATGACTGGTCATACTATGAATACCTTTCCCCTATTTTTTAAATTAGAAGATCGTAAAGTACTGATTGTTGGCGGCGGTGA
TGTGGCATTACGCAAAGCTGATTTGCTGAGCCGTGCTGGTGCGTGTATCACTGTCCTTGCGCCAAGCATTAGCCATGAGA
TACAAGCATTGCTCAGTGATAGTAAGCACGCGCTGATTTATGAGAATTATAATAAAACCTATATGACAGACTCGCGCGTT
ATTATCGCCGCCACTGACGATGAGACGCTCAACCATCAAATACATTCCGATGCGACTGCATTAAATATCCCTGTCAACGT
CGTCGATACGCCACATTTATGCGATTTTATCTTCCCTGCCATTGTCGATCGCAATCCCATCGTGATTGGTATCTCGTCCA
ACGGTAAAGCGCCGGTGCTGGCACGTTTACTGCGGGCACGCCTTGAGACCTTGATTCCGCAAGGTTATGGTAAATTAGCC
AAGCTTGCTGGCGAGTTTCGAGGTGACGTTAAAGCCAAAATTCCAACATTGACTGGGCGTCGGCAATTTTGGGAGCAAGT
ATTTGAAGGTAAAGTCAGTCAATTGATGTTTTCCGGTAATGAAAACGAAGCCATTGCCCAATTGCAAGCTGATTTAGATA
ACACAGCGGCAAATATCACTGCAAAAAATGCTACAGATGAATCAACAGAAGCCCAAAATACGATGGGCGAAGTTTATATC
GTTGGTGCCGGTCCCGGTGACCCAGAATTATTAACCTTTAAAGCATTACGCTTGATGCAGCAAGCGGATATTGTCTATTA
TGATGCGCTGGTATCACCGCAAGTATTGGATTTATGCCGTCGCGATGCCGATAAAGTATTTGTCGGCAAAAAACGCAGTA
ATCATGCCGTTGCACAATTGGGCATCAATGAGCTGTTGGTTAATAGCGCAAAAGAAGGTCGGCGTGTGGTGCGTCTAAAA
GGCGGTGACCCGTTTATCTTTGGGCGTGGCGGTGAAGAGATTGAAAGCCTGCGCTCTCACAATGTCCCTTATCAAGTAGT
GCCGGGCATTACCGCTGCCAATGCCGCTGCTAGCTATGCTGGTATTCCGCTAACCCATCGTGACCATTCGCAGTCAGTAC
GTTTTGTGACAGGGTTTTTAAAGGCAGGCGCGCCTAATAATAACTTCAAAAGTTTTTTGAATACTGATGAAACAGTCGTA
TTCTATATGGGTCTGCATTCGCTACCGCGCTTGACTCAAGGTTTGATTGATGCCGGACGTAGTGCAAAGACCCCGATTGC
CATCGTCTCTAACGCCAGTATGCCCAATCAGCAAGTATTAACGGGTACACTTGCTAGCATCGTTGAATTACAAGCACAAC
ATCAACTACCTACGCCTGCTCTACTCATCATGGGTGATGTCGTAAGCTTACATCATGATTTGGCTTGGTATAATAATAAA
AAGACCTCAGAAAACGATAATAACTGGTTACGAGGCGGTACTGCTACGACACCAAAACCAAATCCTAACCAGCAAGCGCA
TGCGCTATCTATGATTGCTAATTTGGCAACAGAAGATGGTGGTCTTGAGCAGTTAGTGATTGATTAG

Upstream 100 bases:

>100_bases
AAAACTACTTTTAATATCTTAAAAACGAATATACTAATGCCAATACATTACGTGAGCCTGCGCAACTGATAACTCATAAT
AGCAGTATATATTCAATTGA

Downstream 100 bases:

>100_bases
AAAGCGCCCTAAAAGCCCCTTTTTATTTAAAGCCCTGCCAATCTAGTGGTAGGGCTTTTTTCTGATTGCAACAGAGCAAA
CTTCTCTAATCCTTGCTAGA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTGHTMNTFPLFFKLEDRKVLIVGGGDVALRKADLLSRAGACITVLAPSISHEIQALLSDSKHALIYENYNKTYMTDSRV
IIAATDDETLNHQIHSDATALNIPVNVVDTPHLCDFIFPAIVDRNPIVIGISSNGKAPVLARLLRARLETLIPQGYGKLA
KLAGEFRGDVKAKIPTLTGRRQFWEQVFEGKVSQLMFSGNENEAIAQLQADLDNTAANITAKNATDESTEAQNTMGEVYI
VGAGPGDPELLTFKALRLMQQADIVYYDALVSPQVLDLCRRDADKVFVGKKRSNHAVAQLGINELLVNSAKEGRRVVRLK
GGDPFIFGRGGEEIESLRSHNVPYQVVPGITAANAAASYAGIPLTHRDHSQSVRFVTGFLKAGAPNNNFKSFLNTDETVV
FYMGLHSLPRLTQGLIDAGRSAKTPIAIVSNASMPNQQVLTGTLASIVELQAQHQLPTPALLIMGDVVSLHHDLAWYNNK
KTSENDNNWLRGGTATTPKPNPNQQAHALSMIANLATEDGGLEQLVID

Sequences:

>Translated_528_residues
MTGHTMNTFPLFFKLEDRKVLIVGGGDVALRKADLLSRAGACITVLAPSISHEIQALLSDSKHALIYENYNKTYMTDSRV
IIAATDDETLNHQIHSDATALNIPVNVVDTPHLCDFIFPAIVDRNPIVIGISSNGKAPVLARLLRARLETLIPQGYGKLA
KLAGEFRGDVKAKIPTLTGRRQFWEQVFEGKVSQLMFSGNENEAIAQLQADLDNTAANITAKNATDESTEAQNTMGEVYI
VGAGPGDPELLTFKALRLMQQADIVYYDALVSPQVLDLCRRDADKVFVGKKRSNHAVAQLGINELLVNSAKEGRRVVRLK
GGDPFIFGRGGEEIESLRSHNVPYQVVPGITAANAAASYAGIPLTHRDHSQSVRFVTGFLKAGAPNNNFKSFLNTDETVV
FYMGLHSLPRLTQGLIDAGRSAKTPIAIVSNASMPNQQVLTGTLASIVELQAQHQLPTPALLIMGDVVSLHHDLAWYNNK
KTSENDNNWLRGGTATTPKPNPNQQAHALSMIANLATEDGGLEQLVID
>Mature_527_residues
TGHTMNTFPLFFKLEDRKVLIVGGGDVALRKADLLSRAGACITVLAPSISHEIQALLSDSKHALIYENYNKTYMTDSRVI
IAATDDETLNHQIHSDATALNIPVNVVDTPHLCDFIFPAIVDRNPIVIGISSNGKAPVLARLLRARLETLIPQGYGKLAK
LAGEFRGDVKAKIPTLTGRRQFWEQVFEGKVSQLMFSGNENEAIAQLQADLDNTAANITAKNATDESTEAQNTMGEVYIV
GAGPGDPELLTFKALRLMQQADIVYYDALVSPQVLDLCRRDADKVFVGKKRSNHAVAQLGINELLVNSAKEGRRVVRLKG
GDPFIFGRGGEEIESLRSHNVPYQVVPGITAANAAASYAGIPLTHRDHSQSVRFVTGFLKAGAPNNNFKSFLNTDETVVF
YMGLHSLPRLTQGLIDAGRSAKTPIAIVSNASMPNQQVLTGTLASIVELQAQHQLPTPALLIMGDVVSLHHDLAWYNNKK
TSENDNNWLRGGTATTPKPNPNQQAHALSMIANLATEDGGLEQLVID

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=474, Percent_Identity=45.5696202531646, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322922, Length=257, Percent_Identity=34.6303501945525, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 57323; Mature: 57192

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHTMNTFPLFFKLEDRKVLIVGGGDVALRKADLLSRAGACITVLAPSISHEIQALLSD
CCCCCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHHCCCEEEEECCHHHHHHHHHHCC
SKHALIYENYNKTYMTDSRVIIAATDDETLNHQIHSDATALNIPVNVVDTPHLCDFIFPA
CCCEEEEECCCCEEEECCEEEEEECCCCHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHH
IVDRNPIVIGISSNGKAPVLARLLRARLETLIPQGYGKLAKLAGEFRGDVKAKIPTLTGR
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCH
RQFWEQVFEGKVSQLMFSGNENEAIAQLQADLDNTAANITAKNATDESTEAQNTMGEVYI
HHHHHHHHHCHHHHEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCEEE
VGAGPGDPELLTFKALRLMQQADIVYYDALVSPQVLDLCRRDADKVFVGKKRSNHAVAQL
EECCCCCCCHHHHHHHHHHHHCCEEEEEHHCCHHHHHHHHCCCCCEEECCCCCCCHHHHH
GINELLVNSAKEGRRVVRLKGGDPFIFGRGGEEIESLRSHNVPYQVVPGITAANAAASYA
CHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCHHHCHHHHC
GIPLTHRDHSQSVRFVTGFLKAGAPNNNFKSFLNTDETVVFYMGLHSLPRLTQGLIDAGR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCC
SAKTPIAIVSNASMPNQQVLTGTLASIVELQAQHQLPTPALLIMGDVVSLHHDLAWYNNK
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
KTSENDNNWLRGGTATTPKPNPNQQAHALSMIANLATEDGGLEQLVID
CCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
TGHTMNTFPLFFKLEDRKVLIVGGGDVALRKADLLSRAGACITVLAPSISHEIQALLSD
CCCCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHHCCCEEEEECCHHHHHHHHHHCC
SKHALIYENYNKTYMTDSRVIIAATDDETLNHQIHSDATALNIPVNVVDTPHLCDFIFPA
CCCEEEEECCCCEEEECCEEEEEECCCCHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHH
IVDRNPIVIGISSNGKAPVLARLLRARLETLIPQGYGKLAKLAGEFRGDVKAKIPTLTGR
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCH
RQFWEQVFEGKVSQLMFSGNENEAIAQLQADLDNTAANITAKNATDESTEAQNTMGEVYI
HHHHHHHHHCHHHHEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCEEE
VGAGPGDPELLTFKALRLMQQADIVYYDALVSPQVLDLCRRDADKVFVGKKRSNHAVAQL
EECCCCCCCHHHHHHHHHHHHCCEEEEEHHCCHHHHHHHHCCCCCEEECCCCCCCHHHHH
GINELLVNSAKEGRRVVRLKGGDPFIFGRGGEEIESLRSHNVPYQVVPGITAANAAASYA
CHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEECCCCHHHCHHHHC
GIPLTHRDHSQSVRFVTGFLKAGAPNNNFKSFLNTDETVVFYMGLHSLPRLTQGLIDAGR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCC
SAKTPIAIVSNASMPNQQVLTGTLASIVELQAQHQLPTPALLIMGDVVSLHHDLAWYNNK
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
KTSENDNNWLRGGTATTPKPNPNQQAHALSMIANLATEDGGLEQLVID
CCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA