Click here to switch to the map view.

The map label for this gene is nasD [H]

Identifier: 93006150

GI number: 93006150

Start: 1616450

End: 1617880

Strand: Direct

Name: nasD [H]

Synonym: Pcryo_1322

Alternate gene names: 93006150

Gene position: 1616450-1617880 (Clockwise)

Preceding gene: 93006149

Following gene: 93006151

Centisome position: 52.83

GC content: 47.59

Gene sequence:

>1431_bases
ATGTCATCAGATAGATCAAAATTAACAATACAGACAACGTCTTATGCTCATTCAAATGCGGGTATTGTCATTATTGGTGC
TGGCATGGCAGGCAGCCGTTTTGCCATTGAGCTGGCACGTGCCCAGCAGCAAAGTAAGTCGCCAAATAACCAAAGACCAT
TACCGATTACCCTTATCAGCAAAGAGCCAGAGGTTGGCTACAACCGCATTATGCTATCACCAGTGCTGGCAGGAGATAGC
GCCTTTGATGATACTTATTTATACCAGCAATCAGATTATGAACAGTTGGGTATTGTTGTCTTAGCAGGGATTGCAGTTAC
CGCTGTGGACACCAAACAACAGTCTATTAATCTTGATAATGGACAGACGATGGTGTATGCCAAGCTGGTTATCGCTACAG
GTTCGAGCGCTCGAGTCATCCCTTTTCCCAATCATGATGCGACAGGTGTGCATGTGTTCCGAGAAGTAGCCGATGTAAAT
GCTTTAATTGAATATGCGCGCCAAGGGAAAAAAGGTCTAGTAATTGGTGGCGGGGTGCTAGGGCTAGAGGCCGCCTGCGC
GTTGGCTTCTCAAGGGGCAGATATGACGGTCATTCATGTAGACAGCTATGTTCTTAACAGGCAGCTTGATTTACCTGCTG
CCCAGCTACTGCAAGCTGAGCTGGCTCGTCGGGGCGTAGGTGTGGAGGTCTCTGCTATTACTGATTGTATTGCGGTCAAT
GACCAGCATGAGGTGAAAGGCTTATCATTGAAGGATGGACGTACCTTGCCGGCAGATTTTATCGTGATGGCAGTCGGGGT
GATGCCCAATATAGCACTGGCGAGAGACAGCGGTCTTGAAGTCAATCGCGGCATCATTGTGGATGATTGCATGCACACGA
GTTGTCCGCATGTCTACGCAGTTGGCGAATGCGTAGAGTTGGATGGTGAGCTATTCGGTATGGTCGCACCTGTTAATCAG
CAAGTGGATACCTTAGTCTCTGTGCTAAAAGACACTCGACTATCGGGCGACAGCTTAGGCTCTAAAGAGAACTGGCAGCC
TTTTCGCAGTACACCGCTGTCATTAAAGCTAAAAGTATCTGGCATCAGTGCCTTTTCAGCAGGGCAAGTCGACTTTGATG
ACGAGGAAGCGGCGGCTATCGAAACCATTACTTATCAGCAAACCAGTATAAATCACTATCACTGCCTCTATCTTAGAAAC
AATAAACTGATCGGCGCTGTACTCTACGGTGAGGTCAATGAGGGCAGTTTCTATAGCCAGCTGATCAACAGCAATAGTGA
TATCGCGCCTATAAAAGCGGATTTAATATTTGGCGAGGCTTACTGTGATTTGGAGAAGGTAACGACAGATAGTGAGGCTA
ACGGAGACATGGATAACGATAGCGATCTGGATTTATTAAGCGCAGCAGACCTAGTAGGAGCGGCGTCATGA

Upstream 100 bases:

>100_bases
TGTTGGCTTATTTCTTATAAGTGCAGGCTTTAAGCACAAAAGATACTCTGACACGCCATTTAATTAATGCTGCGCGTTGC
GGCTAAGGGATTTTTTGCTC

Downstream 100 bases:

>100_bases
GTGAAGTGATCTTGAGTAATAAACCGAATGCTAGTCAAGAATTAGAAAGTGGTATACTTCGATCTAATAACAAATCTGAT
GACATATTCAATCCTTCAAA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSSDRSKLTIQTTSYAHSNAGIVIIGAGMAGSRFAIELARAQQQSKSPNNQRPLPITLISKEPEVGYNRIMLSPVLAGDS
AFDDTYLYQQSDYEQLGIVVLAGIAVTAVDTKQQSINLDNGQTMVYAKLVIATGSSARVIPFPNHDATGVHVFREVADVN
ALIEYARQGKKGLVIGGGVLGLEAACALASQGADMTVIHVDSYVLNRQLDLPAAQLLQAELARRGVGVEVSAITDCIAVN
DQHEVKGLSLKDGRTLPADFIVMAVGVMPNIALARDSGLEVNRGIIVDDCMHTSCPHVYAVGECVELDGELFGMVAPVNQ
QVDTLVSVLKDTRLSGDSLGSKENWQPFRSTPLSLKLKVSGISAFSAGQVDFDDEEAAAIETITYQQTSINHYHCLYLRN
NKLIGAVLYGEVNEGSFYSQLINSNSDIAPIKADLIFGEAYCDLEKVTTDSEANGDMDNDSDLDLLSAADLVGAAS

Sequences:

>Translated_476_residues
MSSDRSKLTIQTTSYAHSNAGIVIIGAGMAGSRFAIELARAQQQSKSPNNQRPLPITLISKEPEVGYNRIMLSPVLAGDS
AFDDTYLYQQSDYEQLGIVVLAGIAVTAVDTKQQSINLDNGQTMVYAKLVIATGSSARVIPFPNHDATGVHVFREVADVN
ALIEYARQGKKGLVIGGGVLGLEAACALASQGADMTVIHVDSYVLNRQLDLPAAQLLQAELARRGVGVEVSAITDCIAVN
DQHEVKGLSLKDGRTLPADFIVMAVGVMPNIALARDSGLEVNRGIIVDDCMHTSCPHVYAVGECVELDGELFGMVAPVNQ
QVDTLVSVLKDTRLSGDSLGSKENWQPFRSTPLSLKLKVSGISAFSAGQVDFDDEEAAAIETITYQQTSINHYHCLYLRN
NKLIGAVLYGEVNEGSFYSQLINSNSDIAPIKADLIFGEAYCDLEKVTTDSEANGDMDNDSDLDLLSAADLVGAAS
>Mature_475_residues
SSDRSKLTIQTTSYAHSNAGIVIIGAGMAGSRFAIELARAQQQSKSPNNQRPLPITLISKEPEVGYNRIMLSPVLAGDSA
FDDTYLYQQSDYEQLGIVVLAGIAVTAVDTKQQSINLDNGQTMVYAKLVIATGSSARVIPFPNHDATGVHVFREVADVNA
LIEYARQGKKGLVIGGGVLGLEAACALASQGADMTVIHVDSYVLNRQLDLPAAQLLQAELARRGVGVEVSAITDCIAVND
QHEVKGLSLKDGRTLPADFIVMAVGVMPNIALARDSGLEVNRGIIVDDCMHTSCPHVYAVGECVELDGELFGMVAPVNQQ
VDTLVSVLKDTRLSGDSLGSKENWQPFRSTPLSLKLKVSGISAFSAGQVDFDDEEAAAIETITYQQTSINHYHCLYLRNN
KLIGAVLYGEVNEGSFYSQLINSNSDIAPIKADLIFGEAYCDLEKVTTDSEANGDMDNDSDLDLLSAADLVGAAS

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI4757732, Length=259, Percent_Identity=27.7992277992278, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI22202629, Length=259, Percent_Identity=27.7992277992278, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI21389617, Length=294, Percent_Identity=26.8707482993197, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI65787454, Length=294, Percent_Identity=26.8707482993197, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI226437568, Length=294, Percent_Identity=26.8707482993197, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789765, Length=409, Percent_Identity=32.0293398533007, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789065, Length=357, Percent_Identity=27.7310924369748, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788892, Length=254, Percent_Identity=26.7716535433071, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=293, Percent_Identity=32.0819112627986, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281359715, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281359713, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639250, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18543267, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639252, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639257, Length=287, Percent_Identity=24.7386759581882, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581020, Length=263, Percent_Identity=28.1368821292776, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585130, Length=284, Percent_Identity=22.5352112676056, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573993, Length=263, Percent_Identity=28.1368821292776, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 50832; Mature: 50701

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDRSKLTIQTTSYAHSNAGIVIIGAGMAGSRFAIELARAQQQSKSPNNQRPLPITLIS
CCCCCCEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
KEPEVGYNRIMLSPVLAGDSAFDDTYLYQQSDYEQLGIVVLAGIAVTAVDTKQQSINLDN
CCCCCCCCEEEEEEEECCCCCCCCCEEECCCCHHHHCEEEEECEEEEEEECCHHEEECCC
GQTMVYAKLVIATGSSARVIPFPNHDATGVHVFREVADVNALIEYARQGKKGLVIGGGVL
CCEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
GLEAACALASQGADMTVIHVDSYVLNRQLDLPAAQLLQAELARRGVGVEVSAITDCIAVN
HHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHEEEEEC
DQHEVKGLSLKDGRTLPADFIVMAVGVMPNIALARDSGLEVNRGIIVDDCMHTSCPHVYA
CCHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCEECCCEEEECHHCCCCCCEEE
VGECVELDGELFGMVAPVNQQVDTLVSVLKDTRLSGDSLGSKENWQPFRSTPLSLKLKVS
ECEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEEE
GISAFSAGQVDFDDEEAAAIETITYQQTSINHYHCLYLRNNKLIGAVLYGEVNEGSFYSQ
CEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCEEEEEEEEEECCCCHHHHH
LINSNSDIAPIKADLIFGEAYCDLEKVTTDSEANGDMDNDSDLDLLSAADLVGAAS
HHCCCCCCCEEEEEEEEECHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SSDRSKLTIQTTSYAHSNAGIVIIGAGMAGSRFAIELARAQQQSKSPNNQRPLPITLIS
CCCCCEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEC
KEPEVGYNRIMLSPVLAGDSAFDDTYLYQQSDYEQLGIVVLAGIAVTAVDTKQQSINLDN
CCCCCCCCEEEEEEEECCCCCCCCCEEECCCCHHHHCEEEEECEEEEEEECCHHEEECCC
GQTMVYAKLVIATGSSARVIPFPNHDATGVHVFREVADVNALIEYARQGKKGLVIGGGVL
CCEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
GLEAACALASQGADMTVIHVDSYVLNRQLDLPAAQLLQAELARRGVGVEVSAITDCIAVN
HHHHHHHHHCCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHEEEEEC
DQHEVKGLSLKDGRTLPADFIVMAVGVMPNIALARDSGLEVNRGIIVDDCMHTSCPHVYA
CCHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCEECCCEEEECHHCCCCCCEEE
VGECVELDGELFGMVAPVNQQVDTLVSVLKDTRLSGDSLGSKENWQPFRSTPLSLKLKVS
ECEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEEEE
GISAFSAGQVDFDDEEAAAIETITYQQTSINHYHCLYLRNNKLIGAVLYGEVNEGSFYSQ
CEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCEEEEEEEEEECCCCHHHHH
LINSNSDIAPIKADLIFGEAYCDLEKVTTDSEANGDMDNDSDLDLLSAADLVGAAS
HHCCCCCCCEEEEEEEEECHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]