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The map label for this gene is tusD [H]

Identifier: 93006002

GI number: 93006002

Start: 1419242

End: 1419673

Strand: Reverse

Name: tusD [H]

Synonym: Pcryo_1174

Alternate gene names: 93006002

Gene position: 1419673-1419242 (Counterclockwise)

Preceding gene: 93006006

Following gene: 93006001

Centisome position: 46.4

GC content: 45.6

Gene sequence:

>432_bases
ATGACACTCTCAACGCCTCTACTTTTGATTACCGCTGACCCAAGCCATCCACTTGCATATATGGCATTACGCTATGCGCG
CGCTTATCTACAAAACGCCTCTTTAGCCGATAATGCGCCTAATAGCGCGGATCATGAAAACTCTGTATTAAACATCTTCT
TTTATGCAGATGCAGCACATACTGCCAATCGTTTAAGGTGGCAATCAGCCGATCAATCAAATATTACCCAAGAGTGGCAG
GCAATGGCAGCACAGTATAATCTTGTACTGCCAGTATGCGTCAGTACGGCACTCAGTCGCGGTATTAGTGATACGGACAA
CAGCAGCCGCCATCAGTTAGAGACAGAAAATCTAGCGACAGGTTTTAAGCTAGTAGGTCTGAGTGAGCTTGCTATGATGA
TGCAAGGCGACTGTCGCTTACTACAATTCTAA

Upstream 100 bases:

>100_bases
AAAAGCACTGAATGATGATACAATATACAGCCACAAGGTCTCAGTTCTTTTTCAATACTCATTTATTCAATGCTTATTAA
TACTATGTGATACCGCCATT

Downstream 100 bases:

>100_bases
TAAAAATGATGGAAATAAAAATTCAGTTTGATACTAATAGGTAGCGACAAACAAGATTAGGAAAACCTCATGAAATTACT
CATTCGGCTGCATACGCCCA

Product: DsrE-like protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D [H]

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MTLSTPLLLITADPSHPLAYMALRYARAYLQNASLADNAPNSADHENSVLNIFFYADAAHTANRLRWQSADQSNITQEWQ
AMAAQYNLVLPVCVSTALSRGISDTDNSSRHQLETENLATGFKLVGLSELAMMMQGDCRLLQF

Sequences:

>Translated_143_residues
MTLSTPLLLITADPSHPLAYMALRYARAYLQNASLADNAPNSADHENSVLNIFFYADAAHTANRLRWQSADQSNITQEWQ
AMAAQYNLVLPVCVSTALSRGISDTDNSSRHQLETENLATGFKLVGLSELAMMMQGDCRLLQF
>Mature_142_residues
TLSTPLLLITADPSHPLAYMALRYARAYLQNASLADNAPNSADHENSVLNIFFYADAAHTANRLRWQSADQSNITQEWQA
MAAQYNLVLPVCVSTALSRGISDTDNSSRHQLETENLATGFKLVGLSELAMMMQGDCRLLQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE [H]

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family [H]

Homologues:

Organism=Escherichia coli, GI1789743, Length=99, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003787
- InterPro:   IPR017463 [H]

Pfam domain/function: PF02635 DrsE [H]

EC number: NA

Molecular weight: Translated: 15808; Mature: 15677

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSTPLLLITADPSHPLAYMALRYARAYLQNASLADNAPNSADHENSVLNIFFYADAAH
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCHH
TANRLRWQSADQSNITQEWQAMAAQYNLVLPVCVSTALSRGISDTDNSSRHQLETENLAT
HHHHHEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHEECHHHHHC
GFKLVGLSELAMMMQGDCRLLQF
CCHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TLSTPLLLITADPSHPLAYMALRYARAYLQNASLADNAPNSADHENSVLNIFFYADAAH
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCHH
TANRLRWQSADQSNITQEWQAMAAQYNLVLPVCVSTALSRGISDTDNSSRHQLETENLAT
HHHHHEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHEECHHHHHC
GFKLVGLSELAMMMQGDCRLLQF
CCHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA