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The map label for this gene is leuA [H]

Identifier: 93005990

GI number: 93005990

Start: 1408063

End: 1409847

Strand: Direct

Name: leuA [H]

Synonym: Pcryo_1162

Alternate gene names: 93005990

Gene position: 1408063-1409847 (Clockwise)

Preceding gene: 93005988

Following gene: 93005991

Centisome position: 46.02

GC content: 44.37

Gene sequence:

>1785_bases
ATGTCTGACCAAGCAACACGCCCTGTAGCGCCTGCCGCTGCAAAGAGCACACCAAAAAAAGCCGCTTTCGATTTCCGTAA
ATATCGCCCTTTTGCATTTGCGCCACTGTTGCCAGATCGCACATGGCCGAGCAAAACGCTAGAGAAATCACCGATATGGG
CAAGTGTGGATCTGCGTGATGGCAATCAGGCATTGATTGATCCGATGACTATTGAACAAAAAATGCGTTTTTTTGAAACC
TTGATAGATGTGGGTTTTAAAGAAATTGAAATAGGTTTTCCCTCAGCGGCACAAGTCGAGTTTGATTTTGCTCGTAAATT
GATTGAAGAAAATCATGTGCCTGATGACGTGACTTTGCAGGTATTGGTGCAGGCACGTGAGCATTTGATTGCTCGAACTT
TTGAATCCTTAAAAGGCGCAAAGCGTGCCATCGTTCATGTTTATAACTCAACTTGCCGTATTCAGCGCGAAAAAGTATAT
GCTAAAGATAAAGCTGAAATCAAAGAGATAGCTGTCACTGGTGCTAAATTATTGGTTGAATATGCTGCTAAATATCCAGA
AACAGAATGGGTATTCCAGTATTCACCTGAGAGCTTTAGCCAAACTGAGACTGAATATGCAGTTGAAGTCTGTGATGCCG
TTTGTGATGTTTGGCGACCCGAGAACGGTCAAGAGGTAATCTTAAACTTACCTGCGACGGTTGAGGCGTCAATGCCCAAT
GTATTCGCTGATCAAGTCGAGTATTTTTGTCGTAATCTTGCCAAGCGTGAGCATGTGACTATTAGCTTGCATACGCATAA
TGACCGAGGCTGTGCGGTGGCGGCGGCTGAGCTTGGTGTGCTTGCTGGCGCAGATCGTATCGAGGGCACCTTACTTGGTA
ATGGCGAGCGTACCGGCAATATGGATATCATGGTCATGGCGATGAATCTGTTTAGCCAAGGTATCGATCCTGAGCTTGAT
TTTAGTAACATGAGTGAGATTGTACAAGTCGTTAGCGAATGCAATAACTTGCCTCTGCATCCACGTCATCCCTATGTAGG
TGAGCTTGTATTTACTGCCTTTAGCGGCTCGCACCAAGACGCGATCAAGAAATGCCTCGACTATAATGAGAAGCATGTGG
AGAAGACAGAAAACGTTTGGGATGTGGCATATTTACCAATTGATCCTGCACATATTGGTCGCAGCTATCAGGATGTGGTG
CGCATCAATAGCCAATCTGGTAAAGGCGGTGTGGCTTATATCTTGCAGCGCAACTATGGCTTCAATTTACCACGTTGGAT
GCAAATTGACTTTAGTGGTGTGGTACAAAAACAAGCTGAAACCACGGCTCGTGAATTGCAGAATGATGAAATCTTACAGA
CCTTTGAAGACACTTATTTACAGCAAGGCAGTTTTGAGCTGTTAGATTATACGGTCAATAATAAAGGCGGCGAAGTCTAC
TTTAACGGTCAAGTTCAGATGAATGGCGATACCATCACTATAGATGGTGATGGTAATGGGCCGTTATCTTCGTTTATCGA
TGGATTGGCGCAGCATACAGGCAAATCAATCCATGTCATTAACTACGCTGAGCATGCAATCAATCCGCAGCATAATAGCG
GTGATGGTATCAATGATGATAACAACAGAGACAATAAGACAAATGCCAATGCTGCTGCTTATATACAGCTAAATGTAGAT
GGTGAAGTTTATTCAGGCATCGGCACTTGTAGCAGCACGGTATCAGCCATGCTAAAGGGCGCATTGTCAGCCTTTGCTCA
AGCAGTTAGTATCGCTGCTGTTTAA

Upstream 100 bases:

>100_bases
TATAATGGCATAAGTTCGTATTATCTCGTATGATAAAGCAGGATTTTGGCATTGATTGCCTCACTAAAAATACCAACCTA
ATGCCATCTAAGGATTGATT

Downstream 100 bases:

>100_bases
TAACCAAGTACTTAAGAATTTAAATATTTAGGCATATAAGTTTACATTTCTATAAGTATAACGTCATGGCTCATTGCGAT
AAGCAAATCAGAGCCATGGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSDQATRPVAPAAAKSTPKKAAFDFRKYRPFAFAPLLPDRTWPSKTLEKSPIWASVDLRDGNQALIDPMTIEQKMRFFET
LIDVGFKEIEIGFPSAAQVEFDFARKLIEENHVPDDVTLQVLVQAREHLIARTFESLKGAKRAIVHVYNSTCRIQREKVY
AKDKAEIKEIAVTGAKLLVEYAAKYPETEWVFQYSPESFSQTETEYAVEVCDAVCDVWRPENGQEVILNLPATVEASMPN
VFADQVEYFCRNLAKREHVTISLHTHNDRGCAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMVMAMNLFSQGIDPELD
FSNMSEIVQVVSECNNLPLHPRHPYVGELVFTAFSGSHQDAIKKCLDYNEKHVEKTENVWDVAYLPIDPAHIGRSYQDVV
RINSQSGKGGVAYILQRNYGFNLPRWMQIDFSGVVQKQAETTARELQNDEILQTFEDTYLQQGSFELLDYTVNNKGGEVY
FNGQVQMNGDTITIDGDGNGPLSSFIDGLAQHTGKSIHVINYAEHAINPQHNSGDGINDDNNRDNKTNANAAAYIQLNVD
GEVYSGIGTCSSTVSAMLKGALSAFAQAVSIAAV

Sequences:

>Translated_594_residues
MSDQATRPVAPAAAKSTPKKAAFDFRKYRPFAFAPLLPDRTWPSKTLEKSPIWASVDLRDGNQALIDPMTIEQKMRFFET
LIDVGFKEIEIGFPSAAQVEFDFARKLIEENHVPDDVTLQVLVQAREHLIARTFESLKGAKRAIVHVYNSTCRIQREKVY
AKDKAEIKEIAVTGAKLLVEYAAKYPETEWVFQYSPESFSQTETEYAVEVCDAVCDVWRPENGQEVILNLPATVEASMPN
VFADQVEYFCRNLAKREHVTISLHTHNDRGCAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMVMAMNLFSQGIDPELD
FSNMSEIVQVVSECNNLPLHPRHPYVGELVFTAFSGSHQDAIKKCLDYNEKHVEKTENVWDVAYLPIDPAHIGRSYQDVV
RINSQSGKGGVAYILQRNYGFNLPRWMQIDFSGVVQKQAETTARELQNDEILQTFEDTYLQQGSFELLDYTVNNKGGEVY
FNGQVQMNGDTITIDGDGNGPLSSFIDGLAQHTGKSIHVINYAEHAINPQHNSGDGINDDNNRDNKTNANAAAYIQLNVD
GEVYSGIGTCSSTVSAMLKGALSAFAQAVSIAAV
>Mature_593_residues
SDQATRPVAPAAAKSTPKKAAFDFRKYRPFAFAPLLPDRTWPSKTLEKSPIWASVDLRDGNQALIDPMTIEQKMRFFETL
IDVGFKEIEIGFPSAAQVEFDFARKLIEENHVPDDVTLQVLVQAREHLIARTFESLKGAKRAIVHVYNSTCRIQREKVYA
KDKAEIKEIAVTGAKLLVEYAAKYPETEWVFQYSPESFSQTETEYAVEVCDAVCDVWRPENGQEVILNLPATVEASMPNV
FADQVEYFCRNLAKREHVTISLHTHNDRGCAVAAAELGVLAGADRIEGTLLGNGERTGNMDIMVMAMNLFSQGIDPELDF
SNMSEIVQVVSECNNLPLHPRHPYVGELVFTAFSGSHQDAIKKCLDYNEKHVEKTENVWDVAYLPIDPAHIGRSYQDVVR
INSQSGKGGVAYILQRNYGFNLPRWMQIDFSGVVQKQAETTARELQNDEILQTFEDTYLQQGSFELLDYTVNNKGGEVYF
NGQVQMNGDTITIDGDGNGPLSSFIDGLAQHTGKSIHVINYAEHAINPQHNSGDGINDDNNRDNKTNANAAAYIQLNVDG
EVYSGIGTCSSTVSAMLKGALSAFAQAVSIAAV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=472, Percent_Identity=23.3050847457627, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324682, Length=509, Percent_Identity=49.705304518664, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324225, Length=548, Percent_Identity=47.4452554744526, Blast_Score=531, Evalue=1e-151,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 65883; Mature: 65751

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQATRPVAPAAAKSTPKKAAFDFRKYRPFAFAPLLPDRTWPSKTLEKSPIWASVDLRD
CCCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECC
GNQALIDPMTIEQKMRFFETLIDVGFKEIEIGFPSAAQVEFDFARKLIEENHVPDDVTLQ
CCCEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCCHHHHH
VLVQAREHLIARTFESLKGAKRAIVHVYNSTCRIQREKVYAKDKAEIKEIAVTGAKLLVE
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHEEEHHHHHHCCHHHHHHHHHHHHHHHHH
YAAKYPETEWVFQYSPESFSQTETEYAVEVCDAVCDVWRPENGQEVILNLPATVEASMPN
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCH
VFADQVEYFCRNLAKREHVTISLHTHNDRGCAVAAAELGVLAGADRIEGTLLGNGERTGN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCCCC
MDIMVMAMNLFSQGIDPELDFSNMSEIVQVVSECNNLPLHPRHPYVGELVFTAFSGSHQD
CEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCCCCHH
AIKKCLDYNEKHVEKTENVWDVAYLPIDPAHIGRSYQDVVRINSQSGKGGVAYILQRNYG
HHHHHHCCCHHHHHHHCCCEEEEEECCCHHHHCCCHHHHHEECCCCCCCCEEEEEEECCC
FNLPRWMQIDFSGVVQKQAETTARELQNDEILQTFEDTYLQQGSFELLDYTVNNKGGEVY
CCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
FNGQVQMNGDTITIDGDGNGPLSSFIDGLAQHTGKSIHVINYAEHAINPQHNSGDGINDD
ECCEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCC
NNRDNKTNANAAAYIQLNVDGEVYSGIGTCSSTVSAMLKGALSAFAQAVSIAAV
CCCCCCCCCCEEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDQATRPVAPAAAKSTPKKAAFDFRKYRPFAFAPLLPDRTWPSKTLEKSPIWASVDLRD
CCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECC
GNQALIDPMTIEQKMRFFETLIDVGFKEIEIGFPSAAQVEFDFARKLIEENHVPDDVTLQ
CCCEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCCHHHHH
VLVQAREHLIARTFESLKGAKRAIVHVYNSTCRIQREKVYAKDKAEIKEIAVTGAKLLVE
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHEEEHHHHHHCCHHHHHHHHHHHHHHHHH
YAAKYPETEWVFQYSPESFSQTETEYAVEVCDAVCDVWRPENGQEVILNLPATVEASMPN
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCH
VFADQVEYFCRNLAKREHVTISLHTHNDRGCAVAAAELGVLAGADRIEGTLLGNGERTGN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCCCC
MDIMVMAMNLFSQGIDPELDFSNMSEIVQVVSECNNLPLHPRHPYVGELVFTAFSGSHQD
CEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCCCCHH
AIKKCLDYNEKHVEKTENVWDVAYLPIDPAHIGRSYQDVVRINSQSGKGGVAYILQRNYG
HHHHHHCCCHHHHHHHCCCEEEEEECCCHHHHCCCHHHHHEECCCCCCCCEEEEEEECCC
FNLPRWMQIDFSGVVQKQAETTARELQNDEILQTFEDTYLQQGSFELLDYTVNNKGGEVY
CCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
FNGQVQMNGDTITIDGDGNGPLSSFIDGLAQHTGKSIHVINYAEHAINPQHNSGDGINDD
ECCEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCC
NNRDNKTNANAAAYIQLNVDGEVYSGIGTCSSTVSAMLKGALSAFAQAVSIAAV
CCCCCCCCCCEEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA