Click here to switch to the map view.

The map label for this gene is moeB [H]

Identifier: 93005986

GI number: 93005986

Start: 1403708

End: 1404502

Strand: Direct

Name: moeB [H]

Synonym: Pcryo_1158

Alternate gene names: 93005986

Gene position: 1403708-1404502 (Clockwise)

Preceding gene: 93005985

Following gene: 93005987

Centisome position: 45.87

GC content: 44.4

Gene sequence:

>795_bases
ATGAGTAATGTAGAAACAGAAATATTGTTAGATGATGAGCTGCTGCGTTATGCGCGTCAAATATTACTTGATGGCTGGGA
TATTGATGCTCAGCTACGACTAAAATCATCACGTGTCGTCATGATAGGCGCAGGTGGGCTAGGTTGCCCAGCTTCTGAAA
CCTTAGTGCGTGCAGGACTTGGGCATATTCACCTAATTGATGACGATGACATTGAAGCCAGTAATTTACAGCGTCAAACA
TTGTTTTTACCGGAGGATATTGGTAAACCTAAAGCTATAACTGCTGCGCAAATGTTGATGCGTATCAATCCTTTAATCAC
AGCGCGCGGTACGATAGCGCGTTTGAGTGAAGACAATGCTTATGAGTTACTTGATATGGCGTGCGGTACGCCAGACCTAT
TGTTAGACTGTACAGATAATTTTGCTACCCGTGACATCATAAATCGTATTAGTGTGCGCTATCAGATTCCATTATTATCG
GCATCTGCTATTGCAATGCAAGGGCAACTGGCACTGTTTGAGCCAGATCTAAATACCGGCTGCTATCACTGCGTGTTTGG
TTCGGTAGTACTGGATGCCGAGGCGGATGAACGTACGTGTGCCAATTCTGGGGTGCTTGCCAGCACTACAGCTATCATGG
GTAATCTACAAGCCAATGCTGCTTTGCAATATCTAGGATTAACCAAAAATCCACTGACAAACAAACTGCTGATATGGGAT
GGTAGTCGGATGCAGCAACGCTTGATGGGATATCGAAAAGAGTCAGAATGTCCTGTCTGTAATAGCCTTTCCTAA

Upstream 100 bases:

>100_bases
CGATAATATACAAACGATTAAAGATTTTGGTGGGAACGATAGAGTGACGCTTGGGCAGTTTAATAAAAATAAGCGGCAAT
ATTGAGTACTGGTAATCCAT

Downstream 100 bases:

>100_bases
TTTTAATTAATGATTTTTTACCAATTATAATCGCCGATTCTCAAATATCCTTCTTATGACCACAGTTGTCTTTTATGAAA
ATTCATCGTCCGCATTTATT

Product: UBA/THIF-type NAD/FAD binding domain-containing protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSNVETEILLDDELLRYARQILLDGWDIDAQLRLKSSRVVMIGAGGLGCPASETLVRAGLGHIHLIDDDDIEASNLQRQT
LFLPEDIGKPKAITAAQMLMRINPLITARGTIARLSEDNAYELLDMACGTPDLLLDCTDNFATRDIINRISVRYQIPLLS
ASAIAMQGQLALFEPDLNTGCYHCVFGSVVLDAEADERTCANSGVLASTTAIMGNLQANAALQYLGLTKNPLTNKLLIWD
GSRMQQRLMGYRKESECPVCNSLS

Sequences:

>Translated_264_residues
MSNVETEILLDDELLRYARQILLDGWDIDAQLRLKSSRVVMIGAGGLGCPASETLVRAGLGHIHLIDDDDIEASNLQRQT
LFLPEDIGKPKAITAAQMLMRINPLITARGTIARLSEDNAYELLDMACGTPDLLLDCTDNFATRDIINRISVRYQIPLLS
ASAIAMQGQLALFEPDLNTGCYHCVFGSVVLDAEADERTCANSGVLASTTAIMGNLQANAALQYLGLTKNPLTNKLLIWD
GSRMQQRLMGYRKESECPVCNSLS
>Mature_263_residues
SNVETEILLDDELLRYARQILLDGWDIDAQLRLKSSRVVMIGAGGLGCPASETLVRAGLGHIHLIDDDDIEASNLQRQTL
FLPEDIGKPKAITAAQMLMRINPLITARGTIARLSEDNAYELLDMACGTPDLLLDCTDNFATRDIINRISVRYQIPLLSA
SAIAMQGQLALFEPDLNTGCYHCVFGSVVLDAEADERTCANSGVLASTTAIMGNLQANAALQYLGLTKNPLTNKLLIWDG
SRMQQRLMGYRKESECPVCNSLS

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=233, Percent_Identity=36.0515021459227, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4885649, Length=150, Percent_Identity=32, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI23510338, Length=182, Percent_Identity=31.3186813186813, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI23510340, Length=182, Percent_Identity=31.3186813186813, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI38045944, Length=69, Percent_Identity=52.1739130434783, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI38045942, Length=69, Percent_Identity=52.1739130434783, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787048, Length=255, Percent_Identity=39.6078431372549, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI87082356, Length=250, Percent_Identity=42, Blast_Score=171, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17540406, Length=247, Percent_Identity=34.0080971659919, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI193203301, Length=155, Percent_Identity=32.258064516129, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI86565433, Length=167, Percent_Identity=29.3413173652695, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=167, Percent_Identity=29.3413173652695, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=254, Percent_Identity=29.9212598425197, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320598, Length=159, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6322639, Length=169, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321965, Length=78, Percent_Identity=43.5897435897436, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582879, Length=255, Percent_Identity=36.078431372549, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24660640, Length=157, Percent_Identity=36.3057324840764, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28573937, Length=164, Percent_Identity=31.0975609756098, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.-

Molecular weight: Translated: 28859; Mature: 28727

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVETEILLDDELLRYARQILLDGWDIDAQLRLKSSRVVMIGAGGLGCPASETLVRAGL
CCCCCEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCC
GHIHLIDDDDIEASNLQRQTLFLPEDIGKPKAITAAQMLMRINPLITARGTIARLSEDNA
CEEEEECCCCCCCCCCCEEEEECCHHCCCCCHHHHHHHHHHHCCCEECCCCEEECCCCCH
YELLDMACGTPDLLLDCTDNFATRDIINRISVRYQIPLLSASAIAMQGQLALFEPDLNTG
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEEEEEEEECCCCEEECCEEEEECCCCCCC
CYHCVFGSVVLDAEADERTCANSGVLASTTAIMGNLQANAALQYLGLTKNPLTNKLLIWD
EEHEEECCEEEECCCCCHHHCCCCEEEEHHHHHCCCCHHHEEEEECCCCCCCCCEEEEEC
GSRMQQRLMGYRKESECPVCNSLS
CHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNVETEILLDDELLRYARQILLDGWDIDAQLRLKSSRVVMIGAGGLGCPASETLVRAGL
CCCCEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCC
GHIHLIDDDDIEASNLQRQTLFLPEDIGKPKAITAAQMLMRINPLITARGTIARLSEDNA
CEEEEECCCCCCCCCCCEEEEECCHHCCCCCHHHHHHHHHHHCCCEECCCCEEECCCCCH
YELLDMACGTPDLLLDCTDNFATRDIINRISVRYQIPLLSASAIAMQGQLALFEPDLNTG
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHEEEEEEEEECCCCEEECCEEEEECCCCCCC
CYHCVFGSVVLDAEADERTCANSGVLASTTAIMGNLQANAALQYLGLTKNPLTNKLLIWD
EEHEEECCEEEECCCCCHHHCCCCEEEEHHHHHCCCCHHHEEEEECCCCCCCCCEEEEEC
GSRMQQRLMGYRKESECPVCNSLS
CHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]