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The map label for this gene is hemK [H]

Identifier: 93005985

GI number: 93005985

Start: 1402766

End: 1403692

Strand: Direct

Name: hemK [H]

Synonym: Pcryo_1157

Alternate gene names: 93005985

Gene position: 1402766-1403692 (Clockwise)

Preceding gene: 93005984

Following gene: 93005986

Centisome position: 45.84

GC content: 42.5

Gene sequence:

>927_bases
ATGACAGCGCAAAAAGCAGCAGACGTAACAGTATGGCAAATCAAGCAGCAAGCGATACAAATGGCGGAAGAATTATCTGT
TTCGCCATTTTGGATAACAGATTGGCTGTTATATATCATTGATAAACCTGCGCTATTTTTAATAACGGATGATAGCTATC
AGCTAAGTGATGTTGAGGTTGAGCGATTTAATGTTGGTGTGACCAAAATGCAGCAAGGTACACCATTGGCTTATTTAACG
GGTATACAGGCATTTTGGTCGCTTGATTTTGCCGTTAATGAACATACCTTAATTCCCAGACCCGATACTGAAGTTTTGGT
TGAGCAAGTACTACATTGGATAAATACTGCCAATGAAGAGTTTGTGCCTAATGCTGCTAACAATAAGCGTCTGCTTGATC
TGGGCACGGGTTCTGGTTGCATTGCCATCAGTCTTGCTCACGAGTTAAAGCTGGCTCGCAAATTGGAACCAGTAAGCTGG
CAAGTCGTTGCTGTTGATTTCTCGCTAGAGGCGCTAAAAATTGCTCAGCATAATGCGGTTAGTAATGCTGTAGATATCGA
GTTTGTCCATAGTAGCTGGTATGATGCACTCTCTACTCAGGATGAACATTTATTCGATGTGATTGTCTCAAATCCGCCCT
ATATTGATGAGGCGGATGAGCATTTAGCAGGTTTATTAGCAGAGCCAATTAGTGCTTTGAGCGCCCCTAATCATGGTTTG
GCTGATATCGAACATATTGTCCATCATGCGCCGCAGTATCTAAAAGTAGGCGGGCTACTAGCGATTGAGCATGGCTTTGA
TCAAGGGCATGCTGCACGCCAGTTATTTGCGGACAATCACTTCGATAATATACAAACGATTAAAGATTTTGGTGGGAACG
ATAGAGTGACGCTTGGGCAGTTTAATAAAAATAAGCGGCAATATTGA

Upstream 100 bases:

>100_bases
AAGCCAATCCTAATTTAACCAATACTAATGAGCCACCGCGCGCATCAGAGTAGGTTAGAGGTTATAAAAATAAAGCAGAT
ATAATTATTAAGAGTCATTT

Downstream 100 bases:

>100_bases
GTACTGGTAATCCATATGAGTAATGTAGAAACAGAAATATTGTTAGATGATGAGCTGCTGCGTTATGCGCGTCAAATATT
ACTTGATGGCTGGGATATTG

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.HindHemKP [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTAQKAADVTVWQIKQQAIQMAEELSVSPFWITDWLLYIIDKPALFLITDDSYQLSDVEVERFNVGVTKMQQGTPLAYLT
GIQAFWSLDFAVNEHTLIPRPDTEVLVEQVLHWINTANEEFVPNAANNKRLLDLGTGSGCIAISLAHELKLARKLEPVSW
QVVAVDFSLEALKIAQHNAVSNAVDIEFVHSSWYDALSTQDEHLFDVIVSNPPYIDEADEHLAGLLAEPISALSAPNHGL
ADIEHIVHHAPQYLKVGGLLAIEHGFDQGHAARQLFADNHFDNIQTIKDFGGNDRVTLGQFNKNKRQY

Sequences:

>Translated_308_residues
MTAQKAADVTVWQIKQQAIQMAEELSVSPFWITDWLLYIIDKPALFLITDDSYQLSDVEVERFNVGVTKMQQGTPLAYLT
GIQAFWSLDFAVNEHTLIPRPDTEVLVEQVLHWINTANEEFVPNAANNKRLLDLGTGSGCIAISLAHELKLARKLEPVSW
QVVAVDFSLEALKIAQHNAVSNAVDIEFVHSSWYDALSTQDEHLFDVIVSNPPYIDEADEHLAGLLAEPISALSAPNHGL
ADIEHIVHHAPQYLKVGGLLAIEHGFDQGHAARQLFADNHFDNIQTIKDFGGNDRVTLGQFNKNKRQY
>Mature_307_residues
TAQKAADVTVWQIKQQAIQMAEELSVSPFWITDWLLYIIDKPALFLITDDSYQLSDVEVERFNVGVTKMQQGTPLAYLTG
IQAFWSLDFAVNEHTLIPRPDTEVLVEQVLHWINTANEEFVPNAANNKRLLDLGTGSGCIAISLAHELKLARKLEPVSWQ
VVAVDFSLEALKIAQHNAVSNAVDIEFVHSSWYDALSTQDEHLFDVIVSNPPYIDEADEHLAGLLAEPISALSAPNHGLA
DIEHIVHHAPQYLKVGGLLAIEHGFDQGHAARQLFADNHFDNIQTIKDFGGNDRVTLGQFNKNKRQY

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=280, Percent_Identity=39.2857142857143, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082085, Length=220, Percent_Identity=30.4545454545455, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 34412; Mature: 34281

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQKAADVTVWQIKQQAIQMAEELSVSPFWITDWLLYIIDKPALFLITDDSYQLSDVEV
CCCCHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCEECCEEE
ERFNVGVTKMQQGTPLAYLTGIQAFWSLDFAVNEHTLIPRPDTEVLVEQVLHWINTANEE
EEEECCCEEECCCCCCHHHHHHHHHHEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCC
FVPNAANNKRLLDLGTGSGCIAISLAHELKLARKLEPVSWQVVAVDFSLEALKIAQHNAV
CCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCC
SNAVDIEFVHSSWYDALSTQDEHLFDVIVSNPPYIDEADEHLAGLLAEPISALSAPNHGL
CCCEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCH
ADIEHIVHHAPQYLKVGGLLAIEHGFDQGHAARQLFADNHFDNIQTIKDFGGNDRVTLGQ
HHHHHHHHCCCCCEECCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEC
FNKNKRQY
CCCCCCCC
>Mature Secondary Structure 
TAQKAADVTVWQIKQQAIQMAEELSVSPFWITDWLLYIIDKPALFLITDDSYQLSDVEV
CCCHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCEECCEEE
ERFNVGVTKMQQGTPLAYLTGIQAFWSLDFAVNEHTLIPRPDTEVLVEQVLHWINTANEE
EEEECCCEEECCCCCCHHHHHHHHHHEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCC
FVPNAANNKRLLDLGTGSGCIAISLAHELKLARKLEPVSWQVVAVDFSLEALKIAQHNAV
CCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCC
SNAVDIEFVHSSWYDALSTQDEHLFDVIVSNPPYIDEADEHLAGLLAEPISALSAPNHGL
CCCEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCH
ADIEHIVHHAPQYLKVGGLLAIEHGFDQGHAARQLFADNHFDNIQTIKDFGGNDRVTLGQ
HHHHHHHHCCCCCEECCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEC
FNKNKRQY
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]