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The map label for this gene is guaD [H]

Identifier: 93005971

GI number: 93005971

Start: 1384871

End: 1386157

Strand: Direct

Name: guaD [H]

Synonym: Pcryo_1143

Alternate gene names: 93005971

Gene position: 1384871-1386157 (Clockwise)

Preceding gene: 93005970

Following gene: 93005973

Centisome position: 45.26

GC content: 44.29

Gene sequence:

>1287_bases
GTGGTTACAGGCGTCAAGGTTTATCCTGAATATATCGCCAATGGTGCCTTGGTGGTCGATGATGCGACTGGTCGCGTTGT
GGATTATGGCAGCTGTGCGTCAATGATGTCACAGTACGGCAACGACAGTCGCCAAGAGGGTCAAGCGCCAGTACAAATTC
ATGATTATCAAGATAAGCTCATTATGCCGGGTTTTATCGATACTCATGTGCATTATCCGCAAATAGATATGATTGCTGCA
TTTGGCGAGCAATTGCTTGATTGGTTGAATAATTATACCTTTGTGACCGAAGCCAATTTTGGCGATGCAAAGATTGCTGA
CGATACGGCAAAATTCTTTTTGAATCAGCTGCTTGCTAACGGTACAACCAGCGCTTTGGTGTTCTCTACTAGCCATCCAC
AGTCAGTAGAGTCGTTCTTTAACGAAAGTAGTCGTTTGAATACGCGTATGATTACGGGTAATGTATTGATGGATCAAAAC
GCGCCTGAGCACTTGTGTGTACCAACAGAGCAAGGGATTCGCGATACGCAGAATATCATCGATAAATGGCATGAGCGCGG
ACGTCAGCATGTGGCGATTACGCCGAGGTTTGCCATTACCTCAACGCCGAAGCAATTACAAATGACGGGAGAGCTATATC
GTAGCTATGACAGCGTTTATCTGCAAACACATTTGGCTGAAAACCTTGATGAAATCGCCTTTGTACGTGAGCTTTATCCC
AATCATAAAGGTTATTTAGACGTTTATCATGATATGGGCTTACTTGGCAGGCGTACGACGTTGGCGCATGGCATTCATCT
CTCGACATCAGAATATGAAGTGCTACGTGATACTGGCACCCAAATAGCCCATTGCCCGACGTCCAATTTATTTTTAGGCA
GTGGACTGTTTGATTTGTCCAAAACTCTAAGCTATACCGGCGTTAGTATCGCAACCGACGTGGGCGCAGGTACTAGCTTA
TCGATGCTGACCACTTTAGCAGAAGCCTATAAAGTTCAGCAATTACAGAGTAATCCGTTATCAGCGCATCAAGGGTTATA
TCAAATCACGTTAGGCAACGCGCAGTCATTGTTATTAGACGATAAAATCGGTAACTTTATGCCCAATAAAGAAGCAGATT
TTGTGGTGATAGATATGGGTGGCACTGATTTAATGGAGCGCCGTATGACACAGACCAAGTCACTTGATGAGCAGTTATTT
GTGCTGATGATGTTAGGTGATGATAGGGTGATTGAAGAAACAATTATCGCTGGCGTGAGTCGTTATAAAAAAGCGGTTGC
TGCATAA

Upstream 100 bases:

>100_bases
GCGTGCCCATTTAGATACGGCCGATTTAACCAATGATAATTTAACCAATGCTGAGCGTCCTAGCATCACAGTGGTAGAAA
AAAACGTCAATTTGCTGCCA

Downstream 100 bases:

>100_bases
AGGGAAGTATTAAAGGTATTTTTTTGCTTAAGTTAGAATGTTTACATAAAAATCAGCGCCTATTTATTAAGACATTTAAA
TCTTAGTAAATAGGCGCTGA

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVTGVKVYPEYIANGALVVDDATGRVVDYGSCASMMSQYGNDSRQEGQAPVQIHDYQDKLIMPGFIDTHVHYPQIDMIAA
FGEQLLDWLNNYTFVTEANFGDAKIADDTAKFFLNQLLANGTTSALVFSTSHPQSVESFFNESSRLNTRMITGNVLMDQN
APEHLCVPTEQGIRDTQNIIDKWHERGRQHVAITPRFAITSTPKQLQMTGELYRSYDSVYLQTHLAENLDEIAFVRELYP
NHKGYLDVYHDMGLLGRRTTLAHGIHLSTSEYEVLRDTGTQIAHCPTSNLFLGSGLFDLSKTLSYTGVSIATDVGAGTSL
SMLTTLAEAYKVQQLQSNPLSAHQGLYQITLGNAQSLLLDDKIGNFMPNKEADFVVIDMGGTDLMERRMTQTKSLDEQLF
VLMMLGDDRVIEETIIAGVSRYKKAVAA

Sequences:

>Translated_428_residues
MVTGVKVYPEYIANGALVVDDATGRVVDYGSCASMMSQYGNDSRQEGQAPVQIHDYQDKLIMPGFIDTHVHYPQIDMIAA
FGEQLLDWLNNYTFVTEANFGDAKIADDTAKFFLNQLLANGTTSALVFSTSHPQSVESFFNESSRLNTRMITGNVLMDQN
APEHLCVPTEQGIRDTQNIIDKWHERGRQHVAITPRFAITSTPKQLQMTGELYRSYDSVYLQTHLAENLDEIAFVRELYP
NHKGYLDVYHDMGLLGRRTTLAHGIHLSTSEYEVLRDTGTQIAHCPTSNLFLGSGLFDLSKTLSYTGVSIATDVGAGTSL
SMLTTLAEAYKVQQLQSNPLSAHQGLYQITLGNAQSLLLDDKIGNFMPNKEADFVVIDMGGTDLMERRMTQTKSLDEQLF
VLMMLGDDRVIEETIIAGVSRYKKAVAA
>Mature_428_residues
MVTGVKVYPEYIANGALVVDDATGRVVDYGSCASMMSQYGNDSRQEGQAPVQIHDYQDKLIMPGFIDTHVHYPQIDMIAA
FGEQLLDWLNNYTFVTEANFGDAKIADDTAKFFLNQLLANGTTSALVFSTSHPQSVESFFNESSRLNTRMITGNVLMDQN
APEHLCVPTEQGIRDTQNIIDKWHERGRQHVAITPRFAITSTPKQLQMTGELYRSYDSVYLQTHLAENLDEIAFVRELYP
NHKGYLDVYHDMGLLGRRTTLAHGIHLSTSEYEVLRDTGTQIAHCPTSNLFLGSGLFDLSKTLSYTGVSIATDVGAGTSL
SMLTTLAEAYKVQQLQSNPLSAHQGLYQITLGNAQSLLLDDKIGNFMPNKEADFVVIDMGGTDLMERRMTQTKSLDEQLF
VLMMLGDDRVIEETIIAGVSRYKKAVAA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=400, Percent_Identity=31.5, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1789249, Length=419, Percent_Identity=48.2100238663485, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319963, Length=328, Percent_Identity=34.7560975609756, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24643849, Length=438, Percent_Identity=34.0182648401827, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 47565; Mature: 47565

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTGVKVYPEYIANGALVVDDATGRVVDYGSCASMMSQYGNDSRQEGQAPVQIHDYQDKL
CCCCCEECHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCCCHHCCCCCEEEECCCCCE
IMPGFIDTHVHYPQIDMIAAFGEQLLDWLNNYTFVTEANFGDAKIADDTAKFFLNQLLAN
ECCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHC
GTTSALVFSTSHPQSVESFFNESSRLNTRMITGNVLMDQNAPEHLCVPTEQGIRDTQNII
CCCCEEEEECCCCHHHHHHHCCCCCCCEEEEECCEEECCCCCCCEECCCHHCCHHHHHHH
DKWHERGRQHVAITPRFAITSTPKQLQMTGELYRSYDSVYLQTHLAENLDEIAFVRELYP
HHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHKGYLDVYHDMGLLGRRTTLAHGIHLSTSEYEVLRDTGTQIAHCPTSNLFLGSGLFDLS
CCCCCEEEHHCCCHHCCHHHHHCCEEECCCHHHHHHHCCCEEEECCCCCEEECCCHHHHH
KTLSYTGVSIATDVGAGTSLSMLTTLAEAYKVQQLQSNPLSAHQGLYQITLGNAQSLLLD
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCEEEEE
DKIGNFMPNKEADFVVIDMGGTDLMERRMTQTKSLDEQLFVLMMLGDDRVIEETIIAGVS
CHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEEEECCCHHHHHHHHHHHH
RYKKAVAA
HHHHHHCC
>Mature Secondary Structure
MVTGVKVYPEYIANGALVVDDATGRVVDYGSCASMMSQYGNDSRQEGQAPVQIHDYQDKL
CCCCCEECHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCCCHHCCCCCEEEECCCCCE
IMPGFIDTHVHYPQIDMIAAFGEQLLDWLNNYTFVTEANFGDAKIADDTAKFFLNQLLAN
ECCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHC
GTTSALVFSTSHPQSVESFFNESSRLNTRMITGNVLMDQNAPEHLCVPTEQGIRDTQNII
CCCCEEEEECCCCHHHHHHHCCCCCCCEEEEECCEEECCCCCCCEECCCHHCCHHHHHHH
DKWHERGRQHVAITPRFAITSTPKQLQMTGELYRSYDSVYLQTHLAENLDEIAFVRELYP
HHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHKGYLDVYHDMGLLGRRTTLAHGIHLSTSEYEVLRDTGTQIAHCPTSNLFLGSGLFDLS
CCCCCEEEHHCCCHHCCHHHHHCCEEECCCHHHHHHHCCCEEEECCCCCEEECCCHHHHH
KTLSYTGVSIATDVGAGTSLSMLTTLAEAYKVQQLQSNPLSAHQGLYQITLGNAQSLLLD
HHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCEEEEE
DKIGNFMPNKEADFVVIDMGGTDLMERRMTQTKSLDEQLFVLMMLGDDRVIEETIIAGVS
CHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEEEECCCHHHHHHHHHHHH
RYKKAVAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]