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The map label for this gene is purF [H]

Identifier: 93005931

GI number: 93005931

Start: 1327330

End: 1328865

Strand: Direct

Name: purF [H]

Synonym: Pcryo_1103

Alternate gene names: 93005931

Gene position: 1327330-1328865 (Clockwise)

Preceding gene: 93005930

Following gene: 93005938

Centisome position: 43.38

GC content: 45.05

Gene sequence:

>1536_bases
ATGTGTGGAGTCGTTGGGGTCGCAGCTCATGAGCCGGTCAACCAAATTTTGTATGATGCCTTAACCATGTTGCAGCACCG
TGGGCAAGATGCGGCAGGTATTGTGACTTTACAGGATGGACGTTTATATTTACGCAAAGAAAACGGCATGGTACGTGACG
TTTTTATGAATCGCCATATGTTAAAGCTGGTCGGTAAATTCGGTATCGGTCATGTGCGTTATCCGACTGCTGGCACGTCA
AGCAGTGCTGAAGCGCAGCCTTTTTATGTCAACTCGCCTTACGGTATCACGCTGGCACATAATGGTAACTTGACCAATGC
TGAGAGCTTGGCGAAGTCGCTTTATCAAGAAGATCGTCGTCATCTAAATACCGACTCAGATTCTGAGGTTTTGCTCAATG
TGCTGGCACATGAAATGCAAAATCTAGGCAAAACCCATCCAACGCCTGCTGACATCTTTGAAGCAGTGACAGCGGTTTAT
GGTCGTGTTGAAGGCGCTTATGGCGTAGTCGCTTTGATTACTGGTCATGGTTTGCTAGCATTTCGTGATCCAAACGGTAT
TCGTCCGCTCATCTTTGGTAAGCGCATGGCTGCCAATGGCGGCACAGAGTACATGGTAGCATCAGAATCGGTTGCTTTGA
CCGGTTCAGGTTTTAGTATTGTTCGTGATGTTAAGCCCGGCGAAGCTATATTTATTGATTTAGACCATAAATTACATACG
CATCAGTGCGTTGAGCAAAAAGAATATACACCTTGTATCTTTGAATATGTGTATTTTGCCCGTCCAGATTCTATTATGGA
CAATATCTCCGTTTATAAATCACGCCTACGTATGGGTGAAAAACTGGCCGATAAAATCCTTGCTGAATGGGGTGAAGATC
ATGATATCGATGTGGTCATTCCTATTCCTGATACCTCGCGTACCTCAGCGATGGAGCTAGCGCTAAAGATGAATATCAAG
TACCGTGAAGGGTTTATGAAAAACCGCTATATCGGTCGCACCTTTATCATGCCAGGTCAGCAGCAGCGCAAAAAGTCAGT
GCGTCAAAAGCTTAGTGCGGTACCATTAGAGTTTAAAGGTAAAAACGTACTCTTGGTTGACGATTCAATCGTCCGCGGTA
CAACCTGCCATGAGATTATCCAAATGGCACGCGATGCAGGCGCGCGCAAGGTATTCTTTGCCAGTGCAGCGCCACCGGTT
AAGTATCCAAATGTCTATGGTATTGATATGCCAGTGCGTAGCGAACTTATTGCGTCAGGACATAGCGTTGAAGAAGTGCG
TGATATCATCGGTGCTGACCGCTTAATTTTCCAAGATTTGGACGATTTGATTGAAGCGGTCAAAGACACTAAGCATAGTA
AAGTTGAAGGTTTTGATTGTGCCGTATTTAATGGCTGCTACATCACTGGTCAAATCAATGAAGCGTATCTTGAGCATCTG
CAAGAGCAGCGTAACGACACTGCTAAAAAGGGTAAAAAAGGGCTACAAATAGTCGCTGATACGCCAGTAGATATGATGGG
TGTAGAAGAACTTTAA

Upstream 100 bases:

>100_bases
TGGCGGCATATGATAGGTCACAAACTAAAGCGATTTTATCAGTAGTAATGCCGTTATTGTGTTTTTAATGTGTGGTTCAA
ATTCAAAATAGGATACAACT

Downstream 100 bases:

>100_bases
GCCTCTTTATATCTTAAAATAAAGTAATTAAAAAAGGCTGGATTCTCAATGAATTCAGCCTTTTTGCTATTAAATTTATT
GTCAAAATATATAGTTTTAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MCGVVGVAAHEPVNQILYDALTMLQHRGQDAAGIVTLQDGRLYLRKENGMVRDVFMNRHMLKLVGKFGIGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAESLAKSLYQEDRRHLNTDSDSEVLLNVLAHEMQNLGKTHPTPADIFEAVTAVY
GRVEGAYGVVALITGHGLLAFRDPNGIRPLIFGKRMAANGGTEYMVASESVALTGSGFSIVRDVKPGEAIFIDLDHKLHT
HQCVEQKEYTPCIFEYVYFARPDSIMDNISVYKSRLRMGEKLADKILAEWGEDHDIDVVIPIPDTSRTSAMELALKMNIK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLSAVPLEFKGKNVLLVDDSIVRGTTCHEIIQMARDAGARKVFFASAAPPV
KYPNVYGIDMPVRSELIASGHSVEEVRDIIGADRLIFQDLDDLIEAVKDTKHSKVEGFDCAVFNGCYITGQINEAYLEHL
QEQRNDTAKKGKKGLQIVADTPVDMMGVEEL

Sequences:

>Translated_511_residues
MCGVVGVAAHEPVNQILYDALTMLQHRGQDAAGIVTLQDGRLYLRKENGMVRDVFMNRHMLKLVGKFGIGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAESLAKSLYQEDRRHLNTDSDSEVLLNVLAHEMQNLGKTHPTPADIFEAVTAVY
GRVEGAYGVVALITGHGLLAFRDPNGIRPLIFGKRMAANGGTEYMVASESVALTGSGFSIVRDVKPGEAIFIDLDHKLHT
HQCVEQKEYTPCIFEYVYFARPDSIMDNISVYKSRLRMGEKLADKILAEWGEDHDIDVVIPIPDTSRTSAMELALKMNIK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLSAVPLEFKGKNVLLVDDSIVRGTTCHEIIQMARDAGARKVFFASAAPPV
KYPNVYGIDMPVRSELIASGHSVEEVRDIIGADRLIFQDLDDLIEAVKDTKHSKVEGFDCAVFNGCYITGQINEAYLEHL
QEQRNDTAKKGKKGLQIVADTPVDMMGVEEL
>Mature_511_residues
MCGVVGVAAHEPVNQILYDALTMLQHRGQDAAGIVTLQDGRLYLRKENGMVRDVFMNRHMLKLVGKFGIGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAESLAKSLYQEDRRHLNTDSDSEVLLNVLAHEMQNLGKTHPTPADIFEAVTAVY
GRVEGAYGVVALITGHGLLAFRDPNGIRPLIFGKRMAANGGTEYMVASESVALTGSGFSIVRDVKPGEAIFIDLDHKLHT
HQCVEQKEYTPCIFEYVYFARPDSIMDNISVYKSRLRMGEKLADKILAEWGEDHDIDVVIPIPDTSRTSAMELALKMNIK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLSAVPLEFKGKNVLLVDDSIVRGTTCHEIIQMARDAGARKVFFASAAPPV
KYPNVYGIDMPVRSELIASGHSVEEVRDIIGADRLIFQDLDDLIEAVKDTKHSKVEGFDCAVFNGCYITGQINEAYLEHL
QEQRNDTAKKGKKGLQIVADTPVDMMGVEEL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=479, Percent_Identity=36.3256784968685, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1788651, Length=491, Percent_Identity=61.9144602851324, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=30.0925925925926, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=468, Percent_Identity=32.9059829059829, Blast_Score=235, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6323958, Length=495, Percent_Identity=51.5151515151515, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24659598, Length=459, Percent_Identity=35.7298474945534, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=35.5179704016913, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573187, Length=507, Percent_Identity=35.1084812623274, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56723; Mature: 56723

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVVGVAAHEPVNQILYDALTMLQHRGQDAAGIVTLQDGRLYLRKENGMVRDVFMNRHM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEHHHHHHHHH
LKLVGKFGIGHVRYPTAGTSSSAEAQPFYVNSPYGITLAHNGNLTNAESLAKSLYQEDRR
HHHHHHHCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTDSDSEVLLNVLAHEMQNLGKTHPTPADIFEAVTAVYGRVEGAYGVVALITGHGLLA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
FRDPNGIRPLIFGKRMAANGGTEYMVASESVALTGSGFSIVRDVKPGEAIFIDLDHKLHT
EECCCCCCCCEECCEECCCCCCEEEEECCCEEEECCCCEEEEECCCCCEEEEECCCCHHH
HQCVEQKEYTPCIFEYVYFARPDSIMDNISVYKSRLRMGEKLADKILAEWGEDHDIDVVI
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSAMELALKMNIKYREGFMKNRYIGRTFIMPGQQQRKKSVRQKLSAVPLEFKG
ECCCCCCHHHHEEHHEECCHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEECC
KNVLLVDDSIVRGTTCHEIIQMARDAGARKVFFASAAPPVKYPNVYGIDMPVRSELIASG
CEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCC
HSVEEVRDIIGADRLIFQDLDDLIEAVKDTKHSKVEGFDCAVFNGCYITGQINEAYLEHL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCHHHHHHH
QEQRNDTAKKGKKGLQIVADTPVDMMGVEEL
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MCGVVGVAAHEPVNQILYDALTMLQHRGQDAAGIVTLQDGRLYLRKENGMVRDVFMNRHM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCEEHHHHHHHHH
LKLVGKFGIGHVRYPTAGTSSSAEAQPFYVNSPYGITLAHNGNLTNAESLAKSLYQEDRR
HHHHHHHCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTDSDSEVLLNVLAHEMQNLGKTHPTPADIFEAVTAVYGRVEGAYGVVALITGHGLLA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
FRDPNGIRPLIFGKRMAANGGTEYMVASESVALTGSGFSIVRDVKPGEAIFIDLDHKLHT
EECCCCCCCCEECCEECCCCCCEEEEECCCEEEECCCCEEEEECCCCCEEEEECCCCHHH
HQCVEQKEYTPCIFEYVYFARPDSIMDNISVYKSRLRMGEKLADKILAEWGEDHDIDVVI
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSAMELALKMNIKYREGFMKNRYIGRTFIMPGQQQRKKSVRQKLSAVPLEFKG
ECCCCCCHHHHEEHHEECCHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEECC
KNVLLVDDSIVRGTTCHEIIQMARDAGARKVFFASAAPPVKYPNVYGIDMPVRSELIASG
CEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCC
HSVEEVRDIIGADRLIFQDLDDLIEAVKDTKHSKVEGFDCAVFNGCYITGQINEAYLEHL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCHHHHHHH
QEQRNDTAKKGKKGLQIVADTPVDMMGVEEL
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]