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The map label for this gene is gidB [H]

Identifier: 93005885

GI number: 93005885

Start: 1272966

End: 1273682

Strand: Direct

Name: gidB [H]

Synonym: Pcryo_1057

Alternate gene names: 93005885

Gene position: 1272966-1273682 (Clockwise)

Preceding gene: 93005881

Following gene: 93005886

Centisome position: 41.6

GC content: 42.68

Gene sequence:

>717_bases
ATGTCAGACTCCACTAAAGCTTCTTTATCCTCTGCTAACATGGGTAATATCCATATTGATCCGACAGGCTTTGTAAAGCT
TGGCGCACAATTACCGACATTGGCGAATATCTTAGCGCAAGCAATAGACGAGTTGGGTCTAACATTGAGTGCCCAGCAGC
AGCGCAGCTTATTATTGTATTTAGACCAGCTTTTATTATGGAATAAAGCGTATAATCTGACCGCGATTACTGATCCGGTA
GAAGCACTTATCAAACATGTTATCGATTGTTTGGCTATTATAACCCATTTACCCTCGGGGTCGCTGCTCGATATTGGTAC
GGGCGCAGGTTTGCCGGCAGTGATTATTGCTATTTGTCAGCCTGAGCGCTCATGTACAGCGCTTGATAGCAATCAGAAAA
AAATCCGCTTTATTAAGCAAGTGAGCAGTGAGCTTGGTTTGAGTAATATGCAGCCTATCGCATCGCGCATTGAAGCGCAC
GAGGCAAGCTATGATGTGATTACTTCTCGAGCATTTGCCAGTTTGATAGATTTTGTTGCAGTTGCCGAGCCGCGATTGGC
AGATAATGGCTATCTATGTGCGATGAAAGGCAAAGCGCCAAGTGAAGAAGAGTTAGACGCTTTAAGTAACGATTGGCAGT
TTAAGACCATTAAATTAAACGTGCCACGTTTACATGATAGTCGTCATTTAATTGAATTGTCTTATAAAAATGTCTAA

Upstream 100 bases:

>100_bases
AGTATAAGATGACGCACATTGGCGCAATCTGCAACTATTCAATAAGCAGCCTGCATGATAAAATAGACGGTTCACATGTC
AAAAACGGATAAACCCTTGT

Downstream 100 bases:

>100_bases
GCTATGAGCACAGCATTTACCATGAAACTATCTTTTTTATGGTAAATGCTGTGCTCATGTTTATGTTTTCAGTAAAAGAG
CATGTTAATAAAATTAGTAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSDSTKASLSSANMGNIHIDPTGFVKLGAQLPTLANILAQAIDELGLTLSAQQQRSLLLYLDQLLLWNKAYNLTAITDPV
EALIKHVIDCLAIITHLPSGSLLDIGTGAGLPAVIIAICQPERSCTALDSNQKKIRFIKQVSSELGLSNMQPIASRIEAH
EASYDVITSRAFASLIDFVAVAEPRLADNGYLCAMKGKAPSEEELDALSNDWQFKTIKLNVPRLHDSRHLIELSYKNV

Sequences:

>Translated_238_residues
MSDSTKASLSSANMGNIHIDPTGFVKLGAQLPTLANILAQAIDELGLTLSAQQQRSLLLYLDQLLLWNKAYNLTAITDPV
EALIKHVIDCLAIITHLPSGSLLDIGTGAGLPAVIIAICQPERSCTALDSNQKKIRFIKQVSSELGLSNMQPIASRIEAH
EASYDVITSRAFASLIDFVAVAEPRLADNGYLCAMKGKAPSEEELDALSNDWQFKTIKLNVPRLHDSRHLIELSYKNV
>Mature_237_residues
SDSTKASLSSANMGNIHIDPTGFVKLGAQLPTLANILAQAIDELGLTLSAQQQRSLLLYLDQLLLWNKAYNLTAITDPVE
ALIKHVIDCLAIITHLPSGSLLDIGTGAGLPAVIIAICQPERSCTALDSNQKKIRFIKQVSSELGLSNMQPIASRIEAHE
ASYDVITSRAFASLIDFVAVAEPRLADNGYLCAMKGKAPSEEELDALSNDWQFKTIKLNVPRLHDSRHLIELSYKNV

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=201, Percent_Identity=38.8059701492537, Blast_Score=157, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 25876; Mature: 25745

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTKASLSSANMGNIHIDPTGFVKLGAQLPTLANILAQAIDELGLTLSAQQQRSLLLY
CCCCCCCHHCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHH
LDQLLLWNKAYNLTAITDPVEALIKHVIDCLAIITHLPSGSLLDIGTGAGLPAVIIAICQ
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCC
PERSCTALDSNQKKIRFIKQVSSELGLSNMQPIASRIEAHEASYDVITSRAFASLIDFVA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VAEPRLADNGYLCAMKGKAPSEEELDALSNDWQFKTIKLNVPRLHDSRHLIELSYKNV
HCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SDSTKASLSSANMGNIHIDPTGFVKLGAQLPTLANILAQAIDELGLTLSAQQQRSLLLY
CCCCCCHHCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHH
LDQLLLWNKAYNLTAITDPVEALIKHVIDCLAIITHLPSGSLLDIGTGAGLPAVIIAICQ
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCC
PERSCTALDSNQKKIRFIKQVSSELGLSNMQPIASRIEAHEASYDVITSRAFASLIDFVA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VAEPRLADNGYLCAMKGKAPSEEELDALSNDWQFKTIKLNVPRLHDSRHLIELSYKNV
HCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA