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The map label for this gene is ppsA [H]

Identifier: 93005862

GI number: 93005862

Start: 1232266

End: 1234650

Strand: Reverse

Name: ppsA [H]

Synonym: Pcryo_1034

Alternate gene names: 93005862

Gene position: 1234650-1232266 (Counterclockwise)

Preceding gene: 93005865

Following gene: 93005861

Centisome position: 40.35

GC content: 44.11

Gene sequence:

>2385_bases
ATGGCAGCGCAATCTACAGCACTTGTAATTAACCTTGATCAGTTAGGAAAAGACGACATTGAAATGGTCGGTGGCAAAAA
CGCTTCACTTGGTGAAATGATCAGCCATCTATCTGATTTGGGCGTTAGTGTTCCAGGCGGCTTTGCCACAACTTCAAATG
CTTTTTACCAGTTTCTAACAGAAACAGGCTTACTTGATAAAATTAACGGCGAGCTTAAAACCTTAGATGTTAATGATGTC
AATAAACTTGCTGAAACGGGCAAAAAAATCCGTACTTGGATTATTGAGCAAGAACTGCCGAAAGATCTTGAGCAAGCGGT
TCGTGATTCATTCGAAGCAATGAGCGATGGTAAAGAAATCGCGGTTGCGGTACGTTCTTCTGCTACTGCTGAAGATTTGC
CAGATGCCTCATTTGCTGGTCAGCAAGAAACGTTCTTAAACATTCGCGGCATTGATAACGTCCTAATTGCAATTAAAGAA
GTATTTGCATCTCTTTATAATGACCGTGCCATCTCTTATCGTGTACACAAAGGTTTTGAGCATGAAGGCGTAGCATTGTC
TGCTGCTGTACAGCGTATGGTTCGTTCAGAAACTGGTGCTGCTGGCGTTATGTTCACACTAGATACCGAAAGTGGTTTTG
ATCAAGTGGTCTTTATCACTTCAAGCTATGGTCTAGGTGAAATGGTTGTACAGGGCGCCGTAAATCCTGATGAATTCTAT
GTGTCAAAACAATTACTTGCTAACGGCAAACCTGCGATTATCCGTCGCAACTTAGGTAGCAAGCACAAGAAAATGATTTA
TGGTGATGAAGGTAGTACCACCAAATCAGTTAAAGTCATTGATGTCGAAAAAGAAGATCGCATGCAGTTCTCTTTGTCTA
CGGAAGAGCTGACATCACTTGCTAAGCAAGCCATGACCATTGAAAAGCATTATGGTCAAGCAATGGATATCGAGTGGGCA
AAAGACGGCGACACCAATGAAATCTTTATCGTTCAGGCTCGTCCTGAAACGGTTAAGAGTCGCCAAGACAGCAATGTCAT
GGAACGTTATATCATCGACACGACTAACGCTAAAGTATTGTGTGAAGGTCGCTCAATTGGTCAACGTATCGGTTCAGGTA
AAGTCCGTATCGTTAGCAACCTAAATGAGATGGATAAAGTACAAGAAGGCGATGTATTGGTATCAGACATGACGGATCCG
GATTGGGAACCAGTCATGAAGCGTGCTTCTGCTATCATTACCAACCGTGGTGGTCGTACTTGTCACGCAGCGATTATCGC
TCGTGAGCTAGGCGTCCCCGCTATCGTTGGTTGTGGTAATGCCACTGAGCTATTGGTTGATGGTCAAGATGTGACTGCTT
CTTGTGCCGAAGGTGATACTGGCTTCATTTATGAAAGTCAGATTGATTTTGAAGTACAGACCAACTCTGTAGAATCTATG
CCTGAGCTTGCATTCAAAGTCATGATGAACGTTGGTAATCCAGATCGTGCCTTCTCATTTACCCAAATGCCAAACGAAGG
GATTGGTCTTGCGCGTCTTGAGTTCATCATCAACCGTATGATTGGTGTGCATCCAAAAGCATTGCTAAACATGAACAGCT
TGCCACGTGAAATTGCACAAGCCATCAATGAGCGCATCGCAGGTTATGCCTCACCTGTTGACTTCTATGTGGACAAATTG
GTTGAAGGCATCTCAACGCTCGCTGTAGCCTTTATGGATCAGCCTGTTATCGTTCGTATGTCAGATTTCAAATCAAACGA
ATACGCTAACTTGCTTGGTGGTAAGTTATACGAGCCATCAGAAGAAAACCCAATGCTTGGTTTCCGTGGTGCTAGCCGCT
ACGTGTCTGATAACTTCCGTGACTGCTTTGAGCTAGAGTGTAAAGCGCTTAAACGCGTTCGTGATGAGATGGGCTTGACC
AACGTCGAGATTATGATTCCATTCGTCCGTACCGTCGGCGAAGCCAAACAAGTCATCGAATTGCTTGAGAAAAATGGTCT
AAAACGTGGCGAAAATGGTCTACGCATCATCATGATGTGTGAGCTACCAACCAACTGCTTACTAGCAGAAGAGTTCCTAG
AGCATTTCGATGGTTTTTCAATCGGTTCAAACGATTTGACGCAGTTGACTCTAGGTCTTGACCGTGACTCAGGTATCGTC
TCACATCTATTTGATGAGCGTGACCCTGCGGTTAAAAAGCTGTTGTCAATGGCAATTGATGCTTGCCGTAAGCAAAATAA
ATACGTCGGTATCTGTGGTCAAGGTCCATCAGACCATCCAGATCTAGCTTTATGGCTCATGGAGCAAGGCATCAGCTCAG
TATCATTGAACCCTGACTCAGTACTAGATACTTGGTTCTTCTTAGCGGGTGAAGAAGCGAAGTAA

Upstream 100 bases:

>100_bases
TTCATCATTTTTTTGCTTCAAGCTCTCGAAATTCTCAAAATATAGGGGTATAATCGACCATTATAATCTTTACTAAATAA
CCTTATTTTCTGGAGTTATC

Downstream 100 bases:

>100_bases
GCTGTAACGTTACGTCACAACGTTAATTCACACCGTCATTTAAGATACAATTATTAGGGTCTATTTGATTTTTTCAAATA
GACCCTAATATAATGTATGA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MAAQSTALVINLDQLGKDDIEMVGGKNASLGEMISHLSDLGVSVPGGFATTSNAFYQFLTETGLLDKINGELKTLDVNDV
NKLAETGKKIRTWIIEQELPKDLEQAVRDSFEAMSDGKEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKE
VFASLYNDRAISYRVHKGFEHEGVALSAAVQRMVRSETGAAGVMFTLDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFY
VSKQLLANGKPAIIRRNLGSKHKKMIYGDEGSTTKSVKVIDVEKEDRMQFSLSTEELTSLAKQAMTIEKHYGQAMDIEWA
KDGDTNEIFIVQARPETVKSRQDSNVMERYIIDTTNAKVLCEGRSIGQRIGSGKVRIVSNLNEMDKVQEGDVLVSDMTDP
DWEPVMKRASAIITNRGGRTCHAAIIARELGVPAIVGCGNATELLVDGQDVTASCAEGDTGFIYESQIDFEVQTNSVESM
PELAFKVMMNVGNPDRAFSFTQMPNEGIGLARLEFIINRMIGVHPKALLNMNSLPREIAQAINERIAGYASPVDFYVDKL
VEGISTLAVAFMDQPVIVRMSDFKSNEYANLLGGKLYEPSEENPMLGFRGASRYVSDNFRDCFELECKALKRVRDEMGLT
NVEIMIPFVRTVGEAKQVIELLEKNGLKRGENGLRIIMMCELPTNCLLAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGIV
SHLFDERDPAVKKLLSMAIDACRKQNKYVGICGQGPSDHPDLALWLMEQGISSVSLNPDSVLDTWFFLAGEEAK

Sequences:

>Translated_794_residues
MAAQSTALVINLDQLGKDDIEMVGGKNASLGEMISHLSDLGVSVPGGFATTSNAFYQFLTETGLLDKINGELKTLDVNDV
NKLAETGKKIRTWIIEQELPKDLEQAVRDSFEAMSDGKEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKE
VFASLYNDRAISYRVHKGFEHEGVALSAAVQRMVRSETGAAGVMFTLDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFY
VSKQLLANGKPAIIRRNLGSKHKKMIYGDEGSTTKSVKVIDVEKEDRMQFSLSTEELTSLAKQAMTIEKHYGQAMDIEWA
KDGDTNEIFIVQARPETVKSRQDSNVMERYIIDTTNAKVLCEGRSIGQRIGSGKVRIVSNLNEMDKVQEGDVLVSDMTDP
DWEPVMKRASAIITNRGGRTCHAAIIARELGVPAIVGCGNATELLVDGQDVTASCAEGDTGFIYESQIDFEVQTNSVESM
PELAFKVMMNVGNPDRAFSFTQMPNEGIGLARLEFIINRMIGVHPKALLNMNSLPREIAQAINERIAGYASPVDFYVDKL
VEGISTLAVAFMDQPVIVRMSDFKSNEYANLLGGKLYEPSEENPMLGFRGASRYVSDNFRDCFELECKALKRVRDEMGLT
NVEIMIPFVRTVGEAKQVIELLEKNGLKRGENGLRIIMMCELPTNCLLAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGIV
SHLFDERDPAVKKLLSMAIDACRKQNKYVGICGQGPSDHPDLALWLMEQGISSVSLNPDSVLDTWFFLAGEEAK
>Mature_793_residues
AAQSTALVINLDQLGKDDIEMVGGKNASLGEMISHLSDLGVSVPGGFATTSNAFYQFLTETGLLDKINGELKTLDVNDVN
KLAETGKKIRTWIIEQELPKDLEQAVRDSFEAMSDGKEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEV
FASLYNDRAISYRVHKGFEHEGVALSAAVQRMVRSETGAAGVMFTLDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFYV
SKQLLANGKPAIIRRNLGSKHKKMIYGDEGSTTKSVKVIDVEKEDRMQFSLSTEELTSLAKQAMTIEKHYGQAMDIEWAK
DGDTNEIFIVQARPETVKSRQDSNVMERYIIDTTNAKVLCEGRSIGQRIGSGKVRIVSNLNEMDKVQEGDVLVSDMTDPD
WEPVMKRASAIITNRGGRTCHAAIIARELGVPAIVGCGNATELLVDGQDVTASCAEGDTGFIYESQIDFEVQTNSVESMP
ELAFKVMMNVGNPDRAFSFTQMPNEGIGLARLEFIINRMIGVHPKALLNMNSLPREIAQAINERIAGYASPVDFYVDKLV
EGISTLAVAFMDQPVIVRMSDFKSNEYANLLGGKLYEPSEENPMLGFRGASRYVSDNFRDCFELECKALKRVRDEMGLTN
VEIMIPFVRTVGEAKQVIELLEKNGLKRGENGLRIIMMCELPTNCLLAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGIVS
HLFDERDPAVKKLLSMAIDACRKQNKYVGICGQGPSDHPDLALWLMEQGISSVSLNPDSVLDTWFFLAGEEAK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=786, Percent_Identity=65.6488549618321, Blast_Score=1100, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=448, Percent_Identity=27.9017857142857, Blast_Score=129, Evalue=9e-31,
Organism=Escherichia coli, GI1788726, Length=376, Percent_Identity=25.2659574468085, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=25.3658536585366, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994992, Length=371, Percent_Identity=23.989218328841, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=29.535864978903, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87328; Mature: 87197

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQSTALVINLDQLGKDDIEMVGGKNASLGEMISHLSDLGVSVPGGFATTSNAFYQFLT
CCCCCEEEEEEHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ETGLLDKINGELKTLDVNDVNKLAETGKKIRTWIIEQELPKDLEQAVRDSFEAMSDGKEI
HCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
AVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFASLYNDRAISYRVHKGFE
EEEEECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
HEGVALSAAVQRMVRSETGAAGVMFTLDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFY
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHH
VSKQLLANGKPAIIRRNLGSKHKKMIYGDEGSTTKSVKVIDVEKEDRMQFSLSTEELTSL
HHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEECHHHHHHH
AKQAMTIEKHYGQAMDIEWAKDGDTNEIFIVQARPETVKSRQDSNVMERYIIDTTNAKVL
HHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHCCCCHHHHHHHEEECCCCEEE
CEGRSIGQRIGSGKVRIVSNLNEMDKVQEGDVLVSDMTDPDWEPVMKRASAIITNRGGRT
ECCHHHHHHCCCCCEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGVPAIVGCGNATELLVDGQDVTASCAEGDTGFIYESQIDFEVQTNSVESM
HHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEECCCCCCCEEEECEEEEEEECCCHHHH
PELAFKVMMNVGNPDRAFSFTQMPNEGIGLARLEFIINRMIGVHPKALLNMNSLPREIAQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
AINERIAGYASPVDFYVDKLVEGISTLAVAFMDQPVIVRMSDFKSNEYANLLGGKLYEPS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCC
EENPMLGFRGASRYVSDNFRDCFELECKALKRVRDEMGLTNVEIMIPFVRTVGEAKQVIE
CCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
LLEKNGLKRGENGLRIIMMCELPTNCLLAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGIV
HHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SHLFDERDPAVKKLLSMAIDACRKQNKYVGICGQGPSDHPDLALWLMEQGISSVSLNPDS
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEECCCCHH
VLDTWFFLAGEEAK
HHHHHHHCCCCCCC
>Mature Secondary Structure 
AAQSTALVINLDQLGKDDIEMVGGKNASLGEMISHLSDLGVSVPGGFATTSNAFYQFLT
CCCCEEEEEEHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ETGLLDKINGELKTLDVNDVNKLAETGKKIRTWIIEQELPKDLEQAVRDSFEAMSDGKEI
HCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
AVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFASLYNDRAISYRVHKGFE
EEEEECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
HEGVALSAAVQRMVRSETGAAGVMFTLDTESGFDQVVFITSSYGLGEMVVQGAVNPDEFY
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHH
VSKQLLANGKPAIIRRNLGSKHKKMIYGDEGSTTKSVKVIDVEKEDRMQFSLSTEELTSL
HHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEECHHHHHHH
AKQAMTIEKHYGQAMDIEWAKDGDTNEIFIVQARPETVKSRQDSNVMERYIIDTTNAKVL
HHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHCCCCHHHHHHHEEECCCCEEE
CEGRSIGQRIGSGKVRIVSNLNEMDKVQEGDVLVSDMTDPDWEPVMKRASAIITNRGGRT
ECCHHHHHHCCCCCEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGVPAIVGCGNATELLVDGQDVTASCAEGDTGFIYESQIDFEVQTNSVESM
HHHHHHHHHCCCCEEEECCCCCEEEECCCCCEEECCCCCCCEEEECEEEEEEECCCHHHH
PELAFKVMMNVGNPDRAFSFTQMPNEGIGLARLEFIINRMIGVHPKALLNMNSLPREIAQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
AINERIAGYASPVDFYVDKLVEGISTLAVAFMDQPVIVRMSDFKSNEYANLLGGKLYEPS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCC
EENPMLGFRGASRYVSDNFRDCFELECKALKRVRDEMGLTNVEIMIPFVRTVGEAKQVIE
CCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
LLEKNGLKRGENGLRIIMMCELPTNCLLAEEFLEHFDGFSIGSNDLTQLTLGLDRDSGIV
HHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SHLFDERDPAVKKLLSMAIDACRKQNKYVGICGQGPSDHPDLALWLMEQGISSVSLNPDS
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEECCCCHH
VLDTWFFLAGEEAK
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]