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The map label for this gene is pcrA [H]

Identifier: 93005845

GI number: 93005845

Start: 1216556

End: 1218757

Strand: Direct

Name: pcrA [H]

Synonym: Pcryo_1017

Alternate gene names: 93005845

Gene position: 1216556-1218757 (Clockwise)

Preceding gene: 93005843

Following gene: 93005846

Centisome position: 39.76

GC content: 41.42

Gene sequence:

>2202_bases
ATGACCACGACCATTTTTGCTACCCACCATTATAATTATCCGCAAAATTTTATCGAGATGAAACCCAAGATCACGCGGCT
TAAGCAGGCAATTAAGAAGCTCGAAGCCAATCTGATAAATGCGGATGCTGAGGTATTAGCGCAAAGGCTTGCCAGTCATT
TAGGGTTGCAAGTAGATTATGCCAGTGAGCTTAATGCCAATCAGCTATTGGCCGCCACGACTATTGAGGGCAAGGTATTG
GTGATCGCCGGTGCCGGTTCTGGCAAAACCAAAACTCTGACTTATAGAACCAGTTACTTGATAGAAAATGGCGTGTCGCC
AAAAGAGATTTTACTATTAACCTTTACTCGTAAAGCAGCCAACGAAATCAAAGGTCGTGCTAGAGCTTTGCTAGCAAGCA
CACCATCTTTTGCCCAAAGGTTAAATGAGGATTTGGTAGAAGATAAATTGCCAAAAAATAAAGCCCTGAATGACATTACC
AGTGGCACCTTCCATTCTTTTTGTAATATGCTATTGCGTCAGTACTCAGGTCTGCTTGGTATTAATCCGAGATTTACCAT
TCTAGATACAGGCGACAGTGAAGACGCTATCGATTTAATTCATAAGGAAAAAAAGTATCCGACCAAAATAACCAATCAGG
CATTTCCGCGCAAAAAAACGTTGCAAAACATATTTTCAACCTCTAGAAATCGCCGTATTCATATCCGCGATTTAATCGAA
AACAGCTATCCTGATATTGCTGTCCATATTCCTGTTATTGAACAATTGGCCATTGACTTTCATGAGTATAAGCGTGCCAA
TCATTTATACGATTTTGATGACATCATTAGCCAAGTGGTGCGTCATTTAAAGCATAATGATACTTTTCGTCAGCTATTAC
AAAATCAATATCGCTATATCATGGTCGATGAATATCAAGACACCAATATTCCGCAAAAAGAGCTGATTGATCTCATCTGT
GCGCCTACGTCAGTGTCGCTGATGGTCGTCGGTGATGACAACCAAAGTATTTATGCCTTTCGCGGCGCCAATTATGAAAA
TATCTTATTATTCGGAGAGGGTTACCCTGAAGCTAAGCTGATTAAACTTGAGCAAAACTATCGTAGTACGCCAGCAGTGC
TTGATTATATCAATGCTCTGTCTGCTCAGATCACCTTGGGTTACCAAAAGCAGCTGTACTCTGGGGTTTCTATAGAAGGC
GTAAAACCAATATTTCGCCGACTTAGCGATGAAAGCCGCGAGGCAAAGTATATTGCTGATAGAATTATCGAATTAAAACC
AGACCATGATTATCAAGACTTTGCGGTATTGTGCCGAACCTCCTTTCAATCCAACTACGTCCAGCTAGAATTCATGGAGC
GCAATATCCCGTTTATTGTCGTAGGCGGTATTCGTTTTATTGAAAGACGCCATATCAAAGACGTCTTAGCATTTGTCAAA
ATACTTTATAACCCGAATGATACCATTGCATGGCACCGTATTTTGACCTTATTTAAAGGCGTGGGGTCGGTAACTGCCAC
GCGCTTAACCCAAGCAATCAATGCTGACGATAACTCTTTTGAGCCGTTATTAACAACAAAGTTTGCCAAAAAAAGCACGC
AACTTGAGCCGCTGTATAAAATGCTAAGTCAAGCCAGTCAAGCAGAGTCGGTCGAAGCCATTATCGAGCTGATTCTAGAT
TTTTATATGCCCGTTTTAAAAATCAATGAAGACAATTGGCGTGAGCGTAGCGAAGATTTTCGTGTGCTCAAAAATCTAGC
GTTAGAGCATGATAGCCTAGATAAATTTTTAGAAAATCTGGCACTTGATCCACCTAATGACTCGGTTGCCACTGCAGGTG
AGCCTGAAAAAGGCGAAGAAGGTATCGACAAAAACAGGAACAAAGTGACCATTTCGACCATTCATAGTGCCAAAGGACTT
GAATGGCCAGTGGTATTTGTTAATTCATTGGTAGATGGTATGACGCCGCATTACCGCTCACTAAGCGATTTTGAGGAATT
AGAAGAGGAGCGTAAGCTGTTTTATGTTGCTTGTAGTCGTGCTAAAAGCAGGCTGTTTTTAACTGCGCCCGATTATTTTT
CGAGCTATTCAGGCTATTTTGATAAAGTGTCACGGTTTATCGCTGAAGTACCTGCTGATAAGATAACGGTGCTAACGGTA
AAACAGCCTTTAGAAGAGAGTGATGATCCAGTTTGGTGGTGA

Upstream 100 bases:

>100_bases
CTGCCCTAGATTGTCTTACTTATCTAAGACAGCCCCTAAAATTATTATCTTACTTTATAATGAATGGCACGCTAAACCAT
ATCAAAGGTAATATCCTATA

Downstream 100 bases:

>100_bases
TAAAAAATACCATAAAAGCAGGAAAATAGCTTAAAAAATGACTTTTGTTTTTCATTAGGTTGTTACTATTCCTATCAAAA
ATACCTTGCATAAAAACATA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MTTTIFATHHYNYPQNFIEMKPKITRLKQAIKKLEANLINADAEVLAQRLASHLGLQVDYASELNANQLLAATTIEGKVL
VIAGAGSGKTKTLTYRTSYLIENGVSPKEILLLTFTRKAANEIKGRARALLASTPSFAQRLNEDLVEDKLPKNKALNDIT
SGTFHSFCNMLLRQYSGLLGINPRFTILDTGDSEDAIDLIHKEKKYPTKITNQAFPRKKTLQNIFSTSRNRRIHIRDLIE
NSYPDIAVHIPVIEQLAIDFHEYKRANHLYDFDDIISQVVRHLKHNDTFRQLLQNQYRYIMVDEYQDTNIPQKELIDLIC
APTSVSLMVVGDDNQSIYAFRGANYENILLFGEGYPEAKLIKLEQNYRSTPAVLDYINALSAQITLGYQKQLYSGVSIEG
VKPIFRRLSDESREAKYIADRIIELKPDHDYQDFAVLCRTSFQSNYVQLEFMERNIPFIVVGGIRFIERRHIKDVLAFVK
ILYNPNDTIAWHRILTLFKGVGSVTATRLTQAINADDNSFEPLLTTKFAKKSTQLEPLYKMLSQASQAESVEAIIELILD
FYMPVLKINEDNWRERSEDFRVLKNLALEHDSLDKFLENLALDPPNDSVATAGEPEKGEEGIDKNRNKVTISTIHSAKGL
EWPVVFVNSLVDGMTPHYRSLSDFEELEEERKLFYVACSRAKSRLFLTAPDYFSSYSGYFDKVSRFIAEVPADKITVLTV
KQPLEESDDPVWW

Sequences:

>Translated_733_residues
MTTTIFATHHYNYPQNFIEMKPKITRLKQAIKKLEANLINADAEVLAQRLASHLGLQVDYASELNANQLLAATTIEGKVL
VIAGAGSGKTKTLTYRTSYLIENGVSPKEILLLTFTRKAANEIKGRARALLASTPSFAQRLNEDLVEDKLPKNKALNDIT
SGTFHSFCNMLLRQYSGLLGINPRFTILDTGDSEDAIDLIHKEKKYPTKITNQAFPRKKTLQNIFSTSRNRRIHIRDLIE
NSYPDIAVHIPVIEQLAIDFHEYKRANHLYDFDDIISQVVRHLKHNDTFRQLLQNQYRYIMVDEYQDTNIPQKELIDLIC
APTSVSLMVVGDDNQSIYAFRGANYENILLFGEGYPEAKLIKLEQNYRSTPAVLDYINALSAQITLGYQKQLYSGVSIEG
VKPIFRRLSDESREAKYIADRIIELKPDHDYQDFAVLCRTSFQSNYVQLEFMERNIPFIVVGGIRFIERRHIKDVLAFVK
ILYNPNDTIAWHRILTLFKGVGSVTATRLTQAINADDNSFEPLLTTKFAKKSTQLEPLYKMLSQASQAESVEAIIELILD
FYMPVLKINEDNWRERSEDFRVLKNLALEHDSLDKFLENLALDPPNDSVATAGEPEKGEEGIDKNRNKVTISTIHSAKGL
EWPVVFVNSLVDGMTPHYRSLSDFEELEEERKLFYVACSRAKSRLFLTAPDYFSSYSGYFDKVSRFIAEVPADKITVLTV
KQPLEESDDPVWW
>Mature_732_residues
TTTIFATHHYNYPQNFIEMKPKITRLKQAIKKLEANLINADAEVLAQRLASHLGLQVDYASELNANQLLAATTIEGKVLV
IAGAGSGKTKTLTYRTSYLIENGVSPKEILLLTFTRKAANEIKGRARALLASTPSFAQRLNEDLVEDKLPKNKALNDITS
GTFHSFCNMLLRQYSGLLGINPRFTILDTGDSEDAIDLIHKEKKYPTKITNQAFPRKKTLQNIFSTSRNRRIHIRDLIEN
SYPDIAVHIPVIEQLAIDFHEYKRANHLYDFDDIISQVVRHLKHNDTFRQLLQNQYRYIMVDEYQDTNIPQKELIDLICA
PTSVSLMVVGDDNQSIYAFRGANYENILLFGEGYPEAKLIKLEQNYRSTPAVLDYINALSAQITLGYQKQLYSGVSIEGV
KPIFRRLSDESREAKYIADRIIELKPDHDYQDFAVLCRTSFQSNYVQLEFMERNIPFIVVGGIRFIERRHIKDVLAFVKI
LYNPNDTIAWHRILTLFKGVGSVTATRLTQAINADDNSFEPLLTTKFAKKSTQLEPLYKMLSQASQAESVEAIIELILDF
YMPVLKINEDNWRERSEDFRVLKNLALEHDSLDKFLENLALDPPNDSVATAGEPEKGEEGIDKNRNKVTISTIHSAKGLE
WPVVFVNSLVDGMTPHYRSLSDFEELEEERKLFYVACSRAKSRLFLTAPDYFSSYSGYFDKVSRFIAEVPADKITVLTVK
QPLEESDDPVWW

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=676, Percent_Identity=28.5502958579882, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI2367296, Length=688, Percent_Identity=29.5058139534884, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1787196, Length=380, Percent_Identity=26.8421052631579, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=496, Percent_Identity=28.8306451612903, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324477, Length=410, Percent_Identity=26.3414634146341, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83753; Mature: 83622

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTIFATHHYNYPQNFIEMKPKITRLKQAIKKLEANLINADAEVLAQRLASHLGLQVDY
CCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEE
ASELNANQLLAATTIEGKVLVIAGAGSGKTKTLTYRTSYLIENGVSPKEILLLTFTRKAA
CCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEEHHHHHCCCCCHHEEEEEECHHHH
NEIKGRARALLASTPSFAQRLNEDLVEDKLPKNKALNDITSGTFHSFCNMLLRQYSGLLG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCC
INPRFTILDTGDSEDAIDLIHKEKKYPTKITNQAFPRKKTLQNIFSTSRNRRIHIRDLIE
CCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHCCCCCEEEHHHHHC
NSYPDIAVHIPVIEQLAIDFHEYKRANHLYDFDDIISQVVRHLKHNDTFRQLLQNQYRYI
CCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
MVDEYQDTNIPQKELIDLICAPTSVSLMVVGDDNQSIYAFRGANYENILLFGEGYPEAKL
EEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCEE
IKLEQNYRSTPAVLDYINALSAQITLGYQKQLYSGVSIEGVKPIFRRLSDESREAKYIAD
EEEECCCCCCHHHHHHHHHHHHHEEECHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHH
RIIELKPDHDYQDFAVLCRTSFQSNYVQLEFMERNIPFIVVGGIRFIERRHIKDVLAFVK
HHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
ILYNPNDTIAWHRILTLFKGVGSVTATRLTQAINADDNSFEPLLTTKFAKKSTQLEPLYK
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
MLSQASQAESVEAIIELILDFYMPVLKINEDNWRERSEDFRVLKNLALEHDSLDKFLENL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHC
ALDPPNDSVATAGEPEKGEEGIDKNRNKVTISTIHSAKGLEWPVVFVNSLVDGMTPHYRS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
LSDFEELEEERKLFYVACSRAKSRLFLTAPDYFSSYSGYFDKVSRFIAEVPADKITVLTV
HHHHHHHHHHHHEEEEEECCCCCEEEEECCHHHHHHCHHHHHHHHHHHHCCCCCEEEEEE
KQPLEESDDPVWW
CCCCCCCCCCCCC
>Mature Secondary Structure 
TTTIFATHHYNYPQNFIEMKPKITRLKQAIKKLEANLINADAEVLAQRLASHLGLQVDY
CEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEE
ASELNANQLLAATTIEGKVLVIAGAGSGKTKTLTYRTSYLIENGVSPKEILLLTFTRKAA
CCCCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEEHHHHHCCCCCHHEEEEEECHHHH
NEIKGRARALLASTPSFAQRLNEDLVEDKLPKNKALNDITSGTFHSFCNMLLRQYSGLLG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCC
INPRFTILDTGDSEDAIDLIHKEKKYPTKITNQAFPRKKTLQNIFSTSRNRRIHIRDLIE
CCCCEEEEECCCCCHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHCCCCCEEEHHHHHC
NSYPDIAVHIPVIEQLAIDFHEYKRANHLYDFDDIISQVVRHLKHNDTFRQLLQNQYRYI
CCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
MVDEYQDTNIPQKELIDLICAPTSVSLMVVGDDNQSIYAFRGANYENILLFGEGYPEAKL
EEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCEE
IKLEQNYRSTPAVLDYINALSAQITLGYQKQLYSGVSIEGVKPIFRRLSDESREAKYIAD
EEEECCCCCCHHHHHHHHHHHHHEEECHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHH
RIIELKPDHDYQDFAVLCRTSFQSNYVQLEFMERNIPFIVVGGIRFIERRHIKDVLAFVK
HHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
ILYNPNDTIAWHRILTLFKGVGSVTATRLTQAINADDNSFEPLLTTKFAKKSTQLEPLYK
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
MLSQASQAESVEAIIELILDFYMPVLKINEDNWRERSEDFRVLKNLALEHDSLDKFLENL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHC
ALDPPNDSVATAGEPEKGEEGIDKNRNKVTISTIHSAKGLEWPVVFVNSLVDGMTPHYRS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
LSDFEELEEERKLFYVACSRAKSRLFLTAPDYFSSYSGYFDKVSRFIAEVPADKITVLTV
HHHHHHHHHHHHEEEEEECCCCCEEEEECCHHHHHHCHHHHHHHHHHHHCCCCCEEEEEE
KQPLEESDDPVWW
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA