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The map label for this gene is cca [H]

Identifier: 93005824

GI number: 93005824

Start: 1187740

End: 1188912

Strand: Reverse

Name: cca [H]

Synonym: Pcryo_0996

Alternate gene names: 93005824

Gene position: 1188912-1187740 (Counterclockwise)

Preceding gene: 93005825

Following gene: 93005823

Centisome position: 38.85

GC content: 45.01

Gene sequence:

>1173_bases
ATGCAAGTGTATCTCGTCGGCGGTGCAGTACGTGATCAGCTATTAGGGCGCCCTATTAAAGATAAAGACTTCGTTGTCGT
TGGGGCAACAGTTACTGAGATGCTTGAAGCAGGTTTTCAGCAAGTAGGTGCAGATTTCCCTGTATTTTTGCATCCGGTGA
ACCGTGAGGAATATGCTCTCGCACGAACTGAGCGTAAGCAAGGTCTGGGCTATCAGGGCTTTAGCGTGCATGCCAGTATT
GACGTTAGTTTAAAAGACGATTTGCAGCGGCGCGACTTGACGATCAATGCCATGGCCATAGAGGTTACTAGCTTAACCGA
CGATACACCGATAAGTGGTGACGTGATTGATTACTACGGCGGATTGCAGGATATTGAAAATAAAACCCTACGTCATGTCT
CAAGTGCCTTTAACGAAGACCCTTTACGGGTGCTGAGAACGGCGCGCTTTTATAGCCGCTATTACGATTTGGGCTTTACT
ATTGCTGATGACACGTTAGTTTTGATGCGTGAGTTGGTTGCTTCAGGAGAGCTTACCCATTTAAGTAGCGAGCGTATTTG
GCAAGAATCGAGCCGTGCAATGATGCAGCTCTCCCCTCAGGTATACTGGCAACAGCTATTTGATATCGGCGCCCTTGCAG
AGTACTTTGCACCGTTACATCAGGCTTGGAATGATGATCAGCTACGAGAGACAGTGCAAACATCGCTATATTTCGCCGGT
CAGATGCACTTGAATCTATCGCAGCGTTGGGCGCTAGTAATGGTTAGCCTTAAGCCATCATTATTTCAGAACAAACATAC
TGAAGCCATAACAGCGGTCAAAAGTATTAACGATATAGGTAATATGGCTAAAGTACCAAAGGCACAAACACAATTTGCTA
GTTTGTTCGTACAACAAGCTGATAAATTAAGCGTTATAACCCAACTCAGTGCTGCCGAGAAAATTGATCTTATTCAAGCC
TGCAGCGCCCATAAAGAGCCTGAAAAACTCTCGCAGCTCTTGGTCACAAGCCAAGTATTGCAGTTATCTATACAGCACCG
TCAGATGATGTTGGCATTAAATAGCTTTCACAGTATTGGTATGAATGATATTGATCAAAGTCTCAAAGGTCCAGCGATTG
GTAAAGCCTTGCGTCAATGCAGAATTGAGCATTTACAAACGCAAACAGCCTGA

Upstream 100 bases:

>100_bases
GAGACCGTCTGCTATACTAAAACCAGCCAATGCTTAAAGTATTGGCTGGTTTTTTTTGAAAAAATTTAGGTTAATTGGTA
GTAAATATAAAGAGAATATT

Downstream 100 bases:

>100_bases
CTAAAAATAGAGTAAATGACCTTTTAAAACTTAAAAATATATCCTGATTAAAATGCTACTATGAATCAAAAAACTAAAAC
TTCTCAGCCAATTGATACTC

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQVYLVGGAVRDQLLGRPIKDKDFVVVGATVTEMLEAGFQQVGADFPVFLHPVNREEYALARTERKQGLGYQGFSVHASI
DVSLKDDLQRRDLTINAMAIEVTSLTDDTPISGDVIDYYGGLQDIENKTLRHVSSAFNEDPLRVLRTARFYSRYYDLGFT
IADDTLVLMRELVASGELTHLSSERIWQESSRAMMQLSPQVYWQQLFDIGALAEYFAPLHQAWNDDQLRETVQTSLYFAG
QMHLNLSQRWALVMVSLKPSLFQNKHTEAITAVKSINDIGNMAKVPKAQTQFASLFVQQADKLSVITQLSAAEKIDLIQA
CSAHKEPEKLSQLLVTSQVLQLSIQHRQMMLALNSFHSIGMNDIDQSLKGPAIGKALRQCRIEHLQTQTA

Sequences:

>Translated_390_residues
MQVYLVGGAVRDQLLGRPIKDKDFVVVGATVTEMLEAGFQQVGADFPVFLHPVNREEYALARTERKQGLGYQGFSVHASI
DVSLKDDLQRRDLTINAMAIEVTSLTDDTPISGDVIDYYGGLQDIENKTLRHVSSAFNEDPLRVLRTARFYSRYYDLGFT
IADDTLVLMRELVASGELTHLSSERIWQESSRAMMQLSPQVYWQQLFDIGALAEYFAPLHQAWNDDQLRETVQTSLYFAG
QMHLNLSQRWALVMVSLKPSLFQNKHTEAITAVKSINDIGNMAKVPKAQTQFASLFVQQADKLSVITQLSAAEKIDLIQA
CSAHKEPEKLSQLLVTSQVLQLSIQHRQMMLALNSFHSIGMNDIDQSLKGPAIGKALRQCRIEHLQTQTA
>Mature_390_residues
MQVYLVGGAVRDQLLGRPIKDKDFVVVGATVTEMLEAGFQQVGADFPVFLHPVNREEYALARTERKQGLGYQGFSVHASI
DVSLKDDLQRRDLTINAMAIEVTSLTDDTPISGDVIDYYGGLQDIENKTLRHVSSAFNEDPLRVLRTARFYSRYYDLGFT
IADDTLVLMRELVASGELTHLSSERIWQESSRAMMQLSPQVYWQQLFDIGALAEYFAPLHQAWNDDQLRETVQTSLYFAG
QMHLNLSQRWALVMVSLKPSLFQNKHTEAITAVKSINDIGNMAKVPKAQTQFASLFVQQADKLSVITQLSAAEKIDLIQA
CSAHKEPEKLSQLLVTSQVLQLSIQHRQMMLALNSFHSIGMNDIDQSLKGPAIGKALRQCRIEHLQTQTA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=204, Percent_Identity=31.3725490196078, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789436, Length=353, Percent_Identity=45.042492917847, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87081691, Length=207, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281360112, Length=347, Percent_Identity=26.2247838616715, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357337, Length=347, Percent_Identity=26.2247838616715, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43804; Mature: 43804

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYLVGGAVRDQLLGRPIKDKDFVVVGATVTEMLEAGFQQVGADFPVFLHPVNREEYAL
CEEEEECHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
ARTERKQGLGYQGFSVHASIDVSLKDDLQRRDLTINAMAIEVTSLTDDTPISGDVIDYYG
HHHHHHHCCCCCCEEEEEEEEEEHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCHHHHHC
GLQDIENKTLRHVSSAFNEDPLRVLRTARFYSRYYDLGFTIADDTLVLMRELVASGELTH
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCC
LSSERIWQESSRAMMQLSPQVYWQQLFDIGALAEYFAPLHQAWNDDQLRETVQTSLYFAG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
QMHLNLSQRWALVMVSLKPSLFQNKHTEAITAVKSINDIGNMAKVPKAQTQFASLFVQQA
EEEECCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DKLSVITQLSAAEKIDLIQACSAHKEPEKLSQLLVTSQVLQLSIQHRQMMLALNSFHSIG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MNDIDQSLKGPAIGKALRQCRIEHLQTQTA
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVYLVGGAVRDQLLGRPIKDKDFVVVGATVTEMLEAGFQQVGADFPVFLHPVNREEYAL
CEEEEECHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
ARTERKQGLGYQGFSVHASIDVSLKDDLQRRDLTINAMAIEVTSLTDDTPISGDVIDYYG
HHHHHHHCCCCCCEEEEEEEEEEHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCHHHHHC
GLQDIENKTLRHVSSAFNEDPLRVLRTARFYSRYYDLGFTIADDTLVLMRELVASGELTH
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCC
LSSERIWQESSRAMMQLSPQVYWQQLFDIGALAEYFAPLHQAWNDDQLRETVQTSLYFAG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
QMHLNLSQRWALVMVSLKPSLFQNKHTEAITAVKSINDIGNMAKVPKAQTQFASLFVQQA
EEEECCCCCEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DKLSVITQLSAAEKIDLIQACSAHKEPEKLSQLLVTSQVLQLSIQHRQMMLALNSFHSIG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MNDIDQSLKGPAIGKALRQCRIEHLQTQTA
CHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA