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The map label for this gene is kdc [H]

Identifier: 93005792

GI number: 93005792

Start: 1150134

End: 1151804

Strand: Direct

Name: kdc [H]

Synonym: Pcryo_0964

Alternate gene names: 93005792

Gene position: 1150134-1151804 (Clockwise)

Preceding gene: 93005791

Following gene: 93005793

Centisome position: 37.59

GC content: 44.88

Gene sequence:

>1671_bases
ATGAGTCAACAATATACCATCGCTGATTATTTGTTTGATCGTGTCGCAGAAGCAGGTGCGTCAGAGGTATTTGGCGTACC
TGGTGATTTTAATCTCACGTTTTTAGATAATGTGCTTGCCTCAGATAAATTGCGCTGGGTAGGTAATACCAATGAGTTAA
ATGCTGGTTACGCAGCAGATGGCTATGCACGTGAGCGTGGGTTTGCGGCGATGGTGACGACCTTTGGAGTGGGTGAGCTG
TCCGCTATCAATGCCACCGCAGGCTCGTTTGCTGAGTATGCGCCAGTGCTTCATATCGTAGGCGCGCCAAGTACAGCATT
ACAAGATTCAAAGCGTCGCATCCATCACTCACTTGGTGATGGCGTGTTCAATCATTTTATTAAAATGGTAGAACCGGTTA
CGGTAGCACGGGCGCAAATTACGCCTGAAAATGCTGCCTCAGAGATTGACCGCGTGATTCGCGTGATTCTCAAAAAGCAT
CGCCCAGGCTATTTATTACTATCACCAGATGTAGCAAAAACGCCAATTTATCCGCCGACGACTAAACTTATCGATAGTGA
GGAAGATATTACTAGCCAAGCGGCGTTAGCAGACTTTAAACAAGCACTGATAGAATTTTTACCCAATAAAACCACGACGC
TCATGGCAGATTTGATGGTGCATCGTTTGGGTCTGCAAAATCAGCTTAAGGCTTTGATTGCCGATACTGACATTCCTTAT
ACGACGTTATCGTGGGGCAAAACCTTGCTTGATGAAAATAGTGAGCGTTGGGCAGGCACCTATGCAGGCGTGGCATCGCG
TCCTGTGGTTAAGGATATGGTCGAAAACTGTGAGTGTTTGATAAAAATTGGTGTGCAATATACTGATACTACTACTGCAG
GTTTTAGCCAAGATATTGATGAAAACGTGGTGGTTGACCTGCATTATGAGCGTGCGAGTATCGCAGGCAAAAACTTTGCG
CCCATTGCCCTAAAAGATGCTCTAAAAACACTGCATGAGGTAATGACCTCAGATATTAATATTGTACCGAAGCAGTTCTG
TGAAGAGGTAAAGCAGCATGAGCAACATGGCAAGGATAATGAGGCCATTCGTCAAGATGACTTATGGCATATCATTGCTG
ATGCGCTTGATGATAAAAACTTGGTGTTTTCAGAACAAGGTACTGCCTATTTTGGTATCAGTGATGTGCGTCTGCCAGAA
GGCGTGACGTCTTATGGTCAGCCGATGTGGGGTTCTATTGGTTATACTTTGCCTGCGAGTTTAGGTGGTGCCATTGCTTC
ACCGCATAAGCGTAGTATTTTACTCATTGGTGATGGTTCTGCTTTATTAACCATTCAAGAAATTGCGGTAATGATTCAAG
AACGTATCAACCCGGTGATTGTACTGATTAACAATGATGGCTATACCGTTGAGCGCGCGATTCATGGTGAAAACCAGTAC
TATAACGACATTCCTAAATGTGACTGGCAGTTGATGCCAAAAGCGTTTGGTGCCAATGCTAATAATAGTCTGCTACTAAA
AGCAGAGACAGCAGGCGAGTTAAAAGATGCTCTCAAGCAAGCGGCGGCTGCAAAAGACAAGCTGGTTATGTTGGAGGTTA
TCGCTGGCAAGCATGATATCCCGCCATTACTTGCAGATATCAGTGCCGCGCTGAAGCCAAAAAATGATTAA

Upstream 100 bases:

>100_bases
TTCCAAAAGCTTTGCTTTCTATAAATAGGCTGGAATGCAGTTTTATAATTACTTTTATCATATTTATGATTAATAAACAT
AACACTTAAGGATAAGTAGT

Downstream 100 bases:

>100_bases
TTAGTTCGCAAATAGCATCAACAATTATCAGTCATAGCCCCATTTAGCAGCATTGCTAGATGGGTTTTTTTTGAGCCTTT
TTATGGTGTAAGTCAATTAA

Product: pyruvate decarboxylase

Products: aldehyde; CO2

Alternate protein names: KDC [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSQQYTIADYLFDRVAEAGASEVFGVPGDFNLTFLDNVLASDKLRWVGNTNELNAGYAADGYARERGFAAMVTTFGVGEL
SAINATAGSFAEYAPVLHIVGAPSTALQDSKRRIHHSLGDGVFNHFIKMVEPVTVARAQITPENAASEIDRVIRVILKKH
RPGYLLLSPDVAKTPIYPPTTKLIDSEEDITSQAALADFKQALIEFLPNKTTTLMADLMVHRLGLQNQLKALIADTDIPY
TTLSWGKTLLDENSERWAGTYAGVASRPVVKDMVENCECLIKIGVQYTDTTTAGFSQDIDENVVVDLHYERASIAGKNFA
PIALKDALKTLHEVMTSDINIVPKQFCEEVKQHEQHGKDNEAIRQDDLWHIIADALDDKNLVFSEQGTAYFGISDVRLPE
GVTSYGQPMWGSIGYTLPASLGGAIASPHKRSILLIGDGSALLTIQEIAVMIQERINPVIVLINNDGYTVERAIHGENQY
YNDIPKCDWQLMPKAFGANANNSLLLKAETAGELKDALKQAAAAKDKLVMLEVIAGKHDIPPLLADISAALKPKND

Sequences:

>Translated_556_residues
MSQQYTIADYLFDRVAEAGASEVFGVPGDFNLTFLDNVLASDKLRWVGNTNELNAGYAADGYARERGFAAMVTTFGVGEL
SAINATAGSFAEYAPVLHIVGAPSTALQDSKRRIHHSLGDGVFNHFIKMVEPVTVARAQITPENAASEIDRVIRVILKKH
RPGYLLLSPDVAKTPIYPPTTKLIDSEEDITSQAALADFKQALIEFLPNKTTTLMADLMVHRLGLQNQLKALIADTDIPY
TTLSWGKTLLDENSERWAGTYAGVASRPVVKDMVENCECLIKIGVQYTDTTTAGFSQDIDENVVVDLHYERASIAGKNFA
PIALKDALKTLHEVMTSDINIVPKQFCEEVKQHEQHGKDNEAIRQDDLWHIIADALDDKNLVFSEQGTAYFGISDVRLPE
GVTSYGQPMWGSIGYTLPASLGGAIASPHKRSILLIGDGSALLTIQEIAVMIQERINPVIVLINNDGYTVERAIHGENQY
YNDIPKCDWQLMPKAFGANANNSLLLKAETAGELKDALKQAAAAKDKLVMLEVIAGKHDIPPLLADISAALKPKND
>Mature_555_residues
SQQYTIADYLFDRVAEAGASEVFGVPGDFNLTFLDNVLASDKLRWVGNTNELNAGYAADGYARERGFAAMVTTFGVGELS
AINATAGSFAEYAPVLHIVGAPSTALQDSKRRIHHSLGDGVFNHFIKMVEPVTVARAQITPENAASEIDRVIRVILKKHR
PGYLLLSPDVAKTPIYPPTTKLIDSEEDITSQAALADFKQALIEFLPNKTTTLMADLMVHRLGLQNQLKALIADTDIPYT
TLSWGKTLLDENSERWAGTYAGVASRPVVKDMVENCECLIKIGVQYTDTTTAGFSQDIDENVVVDLHYERASIAGKNFAP
IALKDALKTLHEVMTSDINIVPKQFCEEVKQHEQHGKDNEAIRQDDLWHIIADALDDKNLVFSEQGTAYFGISDVRLPEG
VTSYGQPMWGSIGYTLPASLGGAIASPHKRSILLIGDGSALLTIQEIAVMIQERINPVIVLINNDGYTVERAIHGENQYY
NDIPKCDWQLMPKAFGANANNSLLLKAETAGELKDALKQAAAAKDKLVMLEVIAGKHDIPPLLADISAALKPKND

Specific function: Decarboxylates branched-chain and aromatic alpha-keto acids to aldehydes [H]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=20.695652173913, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323163, Length=566, Percent_Identity=35.6890459363958, Blast_Score=319, Evalue=9e-88,
Organism=Saccharomyces cerevisiae, GI6321524, Length=505, Percent_Identity=37.2277227722772, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6323073, Length=566, Percent_Identity=34.6289752650177, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6320123, Length=560, Percent_Identity=29.4642857142857, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6320588, Length=619, Percent_Identity=31.5024232633279, Blast_Score=245, Evalue=1e-65,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 4.1.1.1

Molecular weight: Translated: 60731; Mature: 60599

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQYTIADYLFDRVAEAGASEVFGVPGDFNLTFLDNVLASDKLRWVGNTNELNAGYAAD
CCCCCHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCC
GYARERGFAAMVTTFGVGELSAINATAGSFAEYAPVLHIVGAPSTALQDSKRRIHHSLGD
CHHHHCCCEEEHHHCCCCCCEECCCCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHHHHH
GVFNHFIKMVEPVTVARAQITPENAASEIDRVIRVILKKHRPGYLLLSPDVAKTPIYPPT
HHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
TKLIDSEEDITSQAALADFKQALIEFLPNKTTTLMADLMVHRLGLQNQLKALIADTDIPY
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
TTLSWGKTLLDENSERWAGTYAGVASRPVVKDMVENCECLIKIGVQYTDTTTAGFSQDID
EEHHHHHHHHCCCCCHHCCHHCCCCCCCHHHHHHHCCCEEEEECCEEECCCCCCCCCCCC
ENVVVDLHYERASIAGKNFAPIALKDALKTLHEVMTSDINIVPKQFCEEVKQHEQHGKDN
CCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
EAIRQDDLWHIIADALDDKNLVFSEQGTAYFGISDVRLPEGVTSYGQPMWGSIGYTLPAS
CHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHH
LGGAIASPHKRSILLIGDGSALLTIQEIAVMIQERINPVIVLINNDGYTVERAIHGENQY
CCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCC
YNDIPKCDWQLMPKAFGANANNSLLLKAETAGELKDALKQAAAAKDKLVMLEVIAGKHDI
CCCCCCCCCEECHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PPLLADISAALKPKND
CHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQQYTIADYLFDRVAEAGASEVFGVPGDFNLTFLDNVLASDKLRWVGNTNELNAGYAAD
CCCCHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCC
GYARERGFAAMVTTFGVGELSAINATAGSFAEYAPVLHIVGAPSTALQDSKRRIHHSLGD
CHHHHCCCEEEHHHCCCCCCEECCCCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHHHHH
GVFNHFIKMVEPVTVARAQITPENAASEIDRVIRVILKKHRPGYLLLSPDVAKTPIYPPT
HHHHHHHHHHCCHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
TKLIDSEEDITSQAALADFKQALIEFLPNKTTTLMADLMVHRLGLQNQLKALIADTDIPY
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
TTLSWGKTLLDENSERWAGTYAGVASRPVVKDMVENCECLIKIGVQYTDTTTAGFSQDID
EEHHHHHHHHCCCCCHHCCHHCCCCCCCHHHHHHHCCCEEEEECCEEECCCCCCCCCCCC
ENVVVDLHYERASIAGKNFAPIALKDALKTLHEVMTSDINIVPKQFCEEVKQHEQHGKDN
CCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
EAIRQDDLWHIIADALDDKNLVFSEQGTAYFGISDVRLPEGVTSYGQPMWGSIGYTLPAS
CHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHH
LGGAIASPHKRSILLIGDGSALLTIQEIAVMIQERINPVIVLINNDGYTVERAIHGENQY
CCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCC
YNDIPKCDWQLMPKAFGANANNSLLLKAETAGELKDALKQAAAAKDKLVMLEVIAGKHDI
CCCCCCCCCEECHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PPLLADISAALKPKND
CHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-oxo acid

Specific reaction: A 2-oxo acid = an aldehyde + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]