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The map label for this gene is rluF [H]

Identifier: 93005736

GI number: 93005736

Start: 1075213

End: 1076199

Strand: Direct

Name: rluF [H]

Synonym: Pcryo_0908

Alternate gene names: 93005736

Gene position: 1075213-1076199 (Clockwise)

Preceding gene: 93005732

Following gene: 93005737

Centisome position: 35.14

GC content: 45.09

Gene sequence:

>987_bases
ATGTTTAACGCTTCCTCGACTCGTTTAAATAAATACATCAGTGAAAGCGGTATTTGCTCCAGGCGAGACGCTGACCGCTT
TATTGAACAAGGTAATGTATACCTCAATGGCAAGCGTGCTGCAGTGGGTGATCAAGTGGTTGCTGGAGATACGGTGAAAG
TCAACGGGCAGCTCATTGAGCCGAAAGAAGCTGATGACTTTGTTTTTATTGTATTAAATAAGCCAGTCGGCATTGTGAGC
ACCACGGAAAGCTCCGAGAAAAATAACATTGTTGATTTTGTTAGACATAGCGTACGTATTTTCCCGATTGGCCGTTTGGA
TAAAGACTCTCAAGGTTTGATCTTTTTAACCAGTAATGGCGATCTGGTGAACAAGGTATTGCGTGCTGGTAATAACCATG
AAAAAGAGTATTTGGTGACGGTGAATAAGCCGTTAACGGATAGTGTTATCGAGGGTTTAGCCGGTGGCGTGCCAATGCTT
GGGAAGATGACGAAAAAATGCCCAGTGACTAAAGTAGCGCCCAACGTGTTTAACATCACGCTAGTTCAAGGTCTGAATCG
TCAAATTCGCCGTATGTGTGAGCATTTTGGCTATGAAGTAGTCAAGCTTGAGCGTACGCGGATTATGAATGTAAGTCTCA
AGGGTACGCCCGTTGGAGACTGGCGAGACCTGACGCCAAAAGAGCTGTCTGTTTTACTTAAGTCTGTAGAAGATTCTTCT
TCGCAAGACAGTAAACCGGCTAAGAAATCTCGTAATGCACCACGAAAAAATCCTCGTACTGATGACTCGTCAAAAGCAAA
GTCGTCTTCAAAAACAGCGGGTGCGGCAAAGGGAAATAAAAAACACTCCAAGGATGGTGCCAGAAAACCAGCGGGTTCTA
AAGGTAAAGATTGGCGTCCTTTTAGTGATGGCAAGAAGTCTGGAGGCAAGGGTAAGCCTGCTACAAATGGCAAACGCCCT
TCAAAAGGCCGAGGGTCTAAGCGATAG

Upstream 100 bases:

>100_bases
ATTTCCTATATAATACGAGCCATCTCGTTGTTGATGATCCATTCAATTTAGGCTTTTTTCTGAATAAGAAAGGCTTTTTA
TTAGGTAAAAACGGTGCTTT

Downstream 100 bases:

>100_bases
TAAGTTTTCACAGTGTGACTGCTTTATTCATTTTTTAAATGTCAGCAAGCTAAATAATTTTAACTTTGACCTTAACAGGA
TAATATTATGTTTAAAATAA

Product: 23S rRNA pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MFNASSTRLNKYISESGICSRRDADRFIEQGNVYLNGKRAAVGDQVVAGDTVKVNGQLIEPKEADDFVFIVLNKPVGIVS
TTESSEKNNIVDFVRHSVRIFPIGRLDKDSQGLIFLTSNGDLVNKVLRAGNNHEKEYLVTVNKPLTDSVIEGLAGGVPML
GKMTKKCPVTKVAPNVFNITLVQGLNRQIRRMCEHFGYEVVKLERTRIMNVSLKGTPVGDWRDLTPKELSVLLKSVEDSS
SQDSKPAKKSRNAPRKNPRTDDSSKAKSSSKTAGAAKGNKKHSKDGARKPAGSKGKDWRPFSDGKKSGGKGKPATNGKRP
SKGRGSKR

Sequences:

>Translated_328_residues
MFNASSTRLNKYISESGICSRRDADRFIEQGNVYLNGKRAAVGDQVVAGDTVKVNGQLIEPKEADDFVFIVLNKPVGIVS
TTESSEKNNIVDFVRHSVRIFPIGRLDKDSQGLIFLTSNGDLVNKVLRAGNNHEKEYLVTVNKPLTDSVIEGLAGGVPML
GKMTKKCPVTKVAPNVFNITLVQGLNRQIRRMCEHFGYEVVKLERTRIMNVSLKGTPVGDWRDLTPKELSVLLKSVEDSS
SQDSKPAKKSRNAPRKNPRTDDSSKAKSSSKTAGAAKGNKKHSKDGARKPAGSKGKDWRPFSDGKKSGGKGKPATNGKRP
SKGRGSKR
>Mature_328_residues
MFNASSTRLNKYISESGICSRRDADRFIEQGNVYLNGKRAAVGDQVVAGDTVKVNGQLIEPKEADDFVFIVLNKPVGIVS
TTESSEKNNIVDFVRHSVRIFPIGRLDKDSQGLIFLTSNGDLVNKVLRAGNNHEKEYLVTVNKPLTDSVIEGLAGGVPML
GKMTKKCPVTKVAPNVFNITLVQGLNRQIRRMCEHFGYEVVKLERTRIMNVSLKGTPVGDWRDLTPKELSVLLKSVEDSS
SQDSKPAKKSRNAPRKNPRTDDSSKAKSSSKTAGAAKGNKKHSKDGARKPAGSKGKDWRPFSDGKKSGGKGKPATNGKRP
SKGRGSKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=75.6198347107438, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=31.7647058823529, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=29.9435028248588, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 35777; Mature: 35777

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNASSTRLNKYISESGICSRRDADRFIEQGNVYLNGKRAAVGDQVVAGDTVKVNGQLIE
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEEECCEEEECCEEEC
PKEADDFVFIVLNKPVGIVSTTESSEKNNIVDFVRHSVRIFPIGRLDKDSQGLIFLTSNG
CCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
DLVNKVLRAGNNHEKEYLVTVNKPLTDSVIEGLAGGVPMLGKMTKKCPVTKVAPNVFNIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCEEEH
LVQGLNRQIRRMCEHFGYEVVKLERTRIMNVSLKGTPVGDWRDLTPKELSVLLKSVEDSS
HHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SQDSKPAKKSRNAPRKNPRTDDSSKAKSSSKTAGAAKGNKKHSKDGARKPAGSKGKDWRP
CCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FSDGKKSGGKGKPATNGKRPSKGRGSKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFNASSTRLNKYISESGICSRRDADRFIEQGNVYLNGKRAAVGDQVVAGDTVKVNGQLIE
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEEECCEEEECCEEEC
PKEADDFVFIVLNKPVGIVSTTESSEKNNIVDFVRHSVRIFPIGRLDKDSQGLIFLTSNG
CCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
DLVNKVLRAGNNHEKEYLVTVNKPLTDSVIEGLAGGVPMLGKMTKKCPVTKVAPNVFNIT
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCEEEH
LVQGLNRQIRRMCEHFGYEVVKLERTRIMNVSLKGTPVGDWRDLTPKELSVLLKSVEDSS
HHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
SQDSKPAKKSRNAPRKNPRTDDSSKAKSSSKTAGAAKGNKKHSKDGARKPAGSKGKDWRP
CCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FSDGKKSGGKGKPATNGKRPSKGRGSKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]