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The map label for this gene is rutF [H]

Identifier: 93005731

GI number: 93005731

Start: 1070398

End: 1070913

Strand: Direct

Name: rutF [H]

Synonym: Pcryo_0903

Alternate gene names: 93005731

Gene position: 1070398-1070913 (Clockwise)

Preceding gene: 93005730

Following gene: 93005732

Centisome position: 34.98

GC content: 45.54

Gene sequence:

>516_bases
ATGAGTAAAGATGTGATTGAAGCAACCGACTTTAGAGATGCCATGTCTTTGTTGACGACAGCCGTCAATGTGATCACCAC
CAAAGGCAGCTCAGGTGCTCATGGGTTTACTGCCTCGGCGGTATGTAGCGTCACCGATACACCGCCAACCTTGCTGGTCT
GTATGAATCAAACGTCTCGCTCACATGCCCATTTTCTTGAAAACAAAACCTTAAGTGTCAATGTGTTGGGCGCACAGCAT
GAGCCTATATCTAATGCCTTTGCTTCTAAATTATGCTCAGAAGAGCGATTTCAACAGGGCTCTTGGACTGAGTTAGCAAC
AGGTGCCCCTGTTTTAACAGATGCATTGGTCAGTTTTGACTGTGTGATTGAGGATATTCAGCAAGTTGGTACGCACAGTG
TCTTTATGTGCCGAGTTGTCGCCATCAAACAAGGCTACCAACAAAGCAGTGCTGATGAAAGCTTGGTTTATTTCAACCGT
GCTTACCATCAAGTAGGTCAGGTGGAAATCGTTTAA

Upstream 100 bases:

>100_bases
ATAGGTTGGGTCTTTAACTCCGCCTATAAAAGCTTGTTTAAACTCATGAGTGACCTCAGTTATCAATGTACTTACCGATT
TAACATTTAGGATTGCAGCA

Downstream 100 bases:

>100_bases
GCTGACTTTCTGACATTGATTATCTAATTGTCATCTAACTATAGTTAGCTCAATATCAAGAAGATACCGTGAAACTGGAA
TGAATTTTTTGTAGCCTTTG

Product: flavin reductase-like protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSKDVIEATDFRDAMSLLTTAVNVITTKGSSGAHGFTASAVCSVTDTPPTLLVCMNQTSRSHAHFLENKTLSVNVLGAQH
EPISNAFASKLCSEERFQQGSWTELATGAPVLTDALVSFDCVIEDIQQVGTHSVFMCRVVAIKQGYQQSSADESLVYFNR
AYHQVGQVEIV

Sequences:

>Translated_171_residues
MSKDVIEATDFRDAMSLLTTAVNVITTKGSSGAHGFTASAVCSVTDTPPTLLVCMNQTSRSHAHFLENKTLSVNVLGAQH
EPISNAFASKLCSEERFQQGSWTELATGAPVLTDALVSFDCVIEDIQQVGTHSVFMCRVVAIKQGYQQSSADESLVYFNR
AYHQVGQVEIV
>Mature_170_residues
SKDVIEATDFRDAMSLLTTAVNVITTKGSSGAHGFTASAVCSVTDTPPTLLVCMNQTSRSHAHFLENKTLSVNVLGAQHE
PISNAFASKLCSEERFQQGSWTELATGAPVLTDALVSFDCVIEDIQQVGTHSVFMCRVVAIKQGYQQSSADESLVYFNRA
YHQVGQVEIV

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=162, Percent_Identity=48.7654320987654, Blast_Score=159, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18490; Mature: 18359

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDVIEATDFRDAMSLLTTAVNVITTKGSSGAHGFTASAVCSVTDTPPTLLVCMNQTSR
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEEEECCCCCEEEEEECCCCC
SHAHFLENKTLSVNVLGAQHEPISNAFASKLCSEERFQQGSWTELATGAPVLTDALVSFD
HHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHH
CVIEDIQQVGTHSVFMCRVVAIKQGYQQSSADESLVYFNRAYHQVGQVEIV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
SKDVIEATDFRDAMSLLTTAVNVITTKGSSGAHGFTASAVCSVTDTPPTLLVCMNQTSR
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEEEECCCCCEEEEEECCCCC
SHAHFLENKTLSVNVLGAQHEPISNAFASKLCSEERFQQGSWTELATGAPVLTDALVSFD
HHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHH
CVIEDIQQVGTHSVFMCRVVAIKQGYQQSSADESLVYFNRAYHQVGQVEIV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]