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The map label for this gene is prfB [H]

Identifier: 93005664

GI number: 93005664

Start: 987697

End: 988746

Strand: Direct

Name: prfB [H]

Synonym: Pcryo_0834

Alternate gene names: 93005664

Gene position: 987697-988746 (Clockwise)

Preceding gene: 93005663

Following gene: 93005665

Centisome position: 32.28

GC content: 48.29

Gene sequence:

>1050_bases
GTGGGCAGGACTTGCGGAGGTATCTTTGACTTCGATGGTAAGCAAGAACGCTTAGAAGAAGTCAATTTAGAGTTAGAAAA
CCCCGAGCTATGGAATGATCCAGAACGTGCGACCAAGATTAATAAAGAAAAAAGTCAGCTTGATGGTGTGATTGATGTGG
TGGTGTCGCTTGAAACTACATTGGAAGATGCATCTGCCATGCTGGAGCTGGCGGTAGAAGCAGAGGATGAATCCTTGCTG
GCGGATGTGCAAGCCGAATTAGACAACGCTGAAAAGCGCGTTGCCGATTTAGAGTTTCGCCGGATGTTTAGCGGTGAGAT
GGACCCAAATAATTGCTACTTGGACATCCAGTCAGGTAGTGGAGGTACAGAGGCGCAAGATTGGGCAGAAATGCTACTGC
GTATGTATACGCGCTGGGCGGAAGCGCATGGCTTTAAAGTTGAAGTGATTGAAGTATCATCAGGTAGTGTCGCTGGTATC
AAGTCGGCCACGATTGAGATTAAAGGGAACTATGCCTTTGGTTGGTTACGTACCGAAATCGGCGTCCATCGCTTAGTGCG
TAAGTCACCATTTGATAGCAATAACGGTCGTCATACCTCATTTGCCGCCGTTTTTGTGTCGCCTGAAATTGATGATAATA
TCGAAATTGATATCAATCCAGCAGATTTGCGTATCGATACCTATCGCTCTTCTGGCGCAGGTGGTCAGCACGTAAACACC
ACCGATTCTGCGGTACGTATCACCCACGAGCCAAGTGGCGTAGTGGTCACTTGTCAGAACGAGCGTAGCCAGCATGGTAA
TAAAGCGACGGCGATGAAAATGCTACGTGCTAAGCTCTATGAGCTAGAGATGCAAAAGCGTATGGAATCCCAGCAAGCGG
TTGAAGATGGCAAGTCCGACGTCGGTTGGGGCAGTCAAATCCGCTCTTACGTGCTTGATGACTCTCGTATCAAAGACTTG
CGTACTGGCGTCGAAACCTTTAACACCGGTGCGGTACTCGATGGCGATCTCGATCAATTTATCGAAGCGAGCCTAAAAGC
GGGTCTATAA

Upstream 100 bases:

>100_bases
TGAATCTCATTTAAAATAGCGCTATCGTATCTTTTAAAATATATAGGAACCTTTATGGAAATCAATCCGTATCAAGAACG
AATTAAAGACTTATCTGAGC

Downstream 100 bases:

>100_bases
AGCTGGACTGTAAAAATAGAATATAAAGACAGATTTAAAATATCAACCAACACTTAATTAAGGAAAATTATGCCAAGCGT
TAATAATTATTTTGAAAACA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MGRTCGGIFDFDGKQERLEEVNLELENPELWNDPERATKINKEKSQLDGVIDVVVSLETTLEDASAMLELAVEAEDESLL
ADVQAELDNAEKRVADLEFRRMFSGEMDPNNCYLDIQSGSGGTEAQDWAEMLLRMYTRWAEAHGFKVEVIEVSSGSVAGI
KSATIEIKGNYAFGWLRTEIGVHRLVRKSPFDSNNGRHTSFAAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNT
TDSAVRITHEPSGVVVTCQNERSQHGNKATAMKMLRAKLYELEMQKRMESQQAVEDGKSDVGWGSQIRSYVLDDSRIKDL
RTGVETFNTGAVLDGDLDQFIEASLKAGL

Sequences:

>Translated_349_residues
MGRTCGGIFDFDGKQERLEEVNLELENPELWNDPERATKINKEKSQLDGVIDVVVSLETTLEDASAMLELAVEAEDESLL
ADVQAELDNAEKRVADLEFRRMFSGEMDPNNCYLDIQSGSGGTEAQDWAEMLLRMYTRWAEAHGFKVEVIEVSSGSVAGI
KSATIEIKGNYAFGWLRTEIGVHRLVRKSPFDSNNGRHTSFAAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNT
TDSAVRITHEPSGVVVTCQNERSQHGNKATAMKMLRAKLYELEMQKRMESQQAVEDGKSDVGWGSQIRSYVLDDSRIKDL
RTGVETFNTGAVLDGDLDQFIEASLKAGL
>Mature_348_residues
GRTCGGIFDFDGKQERLEEVNLELENPELWNDPERATKINKEKSQLDGVIDVVVSLETTLEDASAMLELAVEAEDESLLA
DVQAELDNAEKRVADLEFRRMFSGEMDPNNCYLDIQSGSGGTEAQDWAEMLLRMYTRWAEAHGFKVEVIEVSSGSVAGIK
SATIEIKGNYAFGWLRTEIGVHRLVRKSPFDSNNGRHTSFAAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTT
DSAVRITHEPSGVVVTCQNERSQHGNKATAMKMLRAKLYELEMQKRMESQQAVEDGKSDVGWGSQIRSYVLDDSRIKDLR
TGVETFNTGAVLDGDLDQFIEASLKAGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=35.6913183279743, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI34577120, Length=322, Percent_Identity=31.3664596273292, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=35.5140186915888, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=69.9708454810496, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=38.2857142857143, Blast_Score=210, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=287, Percent_Identity=32.0557491289199, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=324, Percent_Identity=35.8024691358025, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19921226, Length=227, Percent_Identity=41.4096916299559, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38689; Mature: 38558

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTCGGIFDFDGKQERLEEVNLELENPELWNDPERATKINKEKSQLDGVIDVVVSLETT
CCCCCCCEECCCCHHHHHHHCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LEDASAMLELAVEAEDESLLADVQAELDNAEKRVADLEFRRMFSGEMDPNNCYLDIQSGS
HHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWAEMLLRMYTRWAEAHGFKVEVIEVSSGSVAGIKSATIEIKGNYAFGWLRTEI
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEECCEEEEEHHHHH
GVHRLVRKSPFDSNNGRHTSFAAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNT
HHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECHHHEEEEECCCCCCCCCCCCC
TDSAVRITHEPSGVVVTCQNERSQHGNKATAMKMLRAKLYELEMQKRMESQQAVEDGKSD
CCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VGWGSQIRSYVLDDSRIKDLRTGVETFNTGAVLDGDLDQFIEASLKAGL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GRTCGGIFDFDGKQERLEEVNLELENPELWNDPERATKINKEKSQLDGVIDVVVSLETT
CCCCCCEECCCCHHHHHHHCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LEDASAMLELAVEAEDESLLADVQAELDNAEKRVADLEFRRMFSGEMDPNNCYLDIQSGS
HHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWAEMLLRMYTRWAEAHGFKVEVIEVSSGSVAGIKSATIEIKGNYAFGWLRTEI
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEECCEEEEEHHHHH
GVHRLVRKSPFDSNNGRHTSFAAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNT
HHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECHHHEEEEECCCCCCCCCCCCC
TDSAVRITHEPSGVVVTCQNERSQHGNKATAMKMLRAKLYELEMQKRMESQQAVEDGKSD
CCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VGWGSQIRSYVLDDSRIKDLRTGVETFNTGAVLDGDLDQFIEASLKAGL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA