Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 93005660

GI number: 93005660

Start: 983848

End: 985080

Strand: Reverse

Name: carA [H]

Synonym: Pcryo_0830

Alternate gene names: 93005660

Gene position: 985080-983848 (Counterclockwise)

Preceding gene: 93005661

Following gene: 93005659

Centisome position: 32.19

GC content: 47.85

Gene sequence:

>1233_bases
TTGAATTCATCGGCAGAAATACAAGCAATTTTGGCACTAGCAGACGGTACGATTTTTCGCGGTTTGAGTATCGGTAGTCT
TGGTCATCGTGTCGGTGAGGTGGTATTTAATACAGCCATGACAGGGTACCAAGAAATCCTAACTGACCCAAGTTACGCGC
GCCAACTGGTCACGCTTACCTATCCGCATATCGGCAATACTGGCACCAACGCTGAAGATGTTGAGTCTGGTAATGGTCAT
CAAGTGTGGGCTGAAGGTCTTATCATCCGCGATGCAACCATGGTGACCTCAAACTTCCGCAACTCAGAGTCACTGAGTGA
TTATTTACAGAGCCATAATACCGTCGCTATTGCTGATATTGATACCCGTCAACTGACCCGCCTACTACGTGATAAAGGCG
CGCAAAACGGCTGTATCATGACGGCTTCTAACGGCGCAACGATTAGCAGCGAAGATGAACAGCAAGCGATTAAATTGGCA
CAAGAATTTGCTGGTATCGAAGGTATGGACTTAGCAAAAGAATGCTGTACACCGACGACATTTGAATGGACCGCTGGTAG
CTGGGATTTATCAGACACTTTACTTAACCGCGATGTAGCTGGTAGCCATGATAGCGGTACAGGCGGCTTCTTTAAGCACT
TGGGTACGCACGTCGAAGCAAAATATAACGTCGTTGCCTATGACTTTGGTAGCAAAACCAATATCCTACGTATGCTCGTT
GACCGCGGTTGTCATGTCACTGTCGTGCCAGCACAAACGCCTATCGCAGATGTCCTCGCGATGAATCCAGATGGTATCTT
CTTATCAAATGGCCCTGGCGATCCAGCTGCCTGTGATTACGCTATCGATGCGGTACGTCATATCATTGAACAAACTGACA
TTCCAACCTTTGGTATCTGCTTAGGTCATCAGCTGATTGGTCTTGCCAGCGGTGCAAAAACCATCAAAATGAAAACTGGT
CATCACGGTGCCAACCATCCGGTACAAGACTTGGCAACTGGCACGGTTATGATTACCAGTCAAAACCATGGTTTTGCGGT
TGATGAAGACACGCTGCCTGCCAATGTTAAATCGACCCATCGCTCATTATTTGACGGTACCAACCAAGGGATTGAGCTGA
CCAATAAGCCAGTCTTTAGTTTCCAAGGTCACCCAGAAGCCAGCCCTGGCCCGCATGATGCTGCGCCACTGTTCGATAAA
TTTGCCGATATGATGGCAGCAGCGAAAGCTTAA

Upstream 100 bases:

>100_bases
TGGTTATTTAGCCGCAGTTAAATAGCAATAATCAAAAAAGCAGCGCTTTATTTTAACCAAAAAACAGCACAGCACTCACT
AATAACAGCGGAGGCAACCC

Downstream 100 bases:

>100_bases
TCCCTAGCCATAAATAATAAGTATAAAGCTTGAAGCACAGATGGTGATTCAAGCTACACCATGCCAGACACGATAAGCGT
TATTGAACATATCGTTAAAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNSSAEIQAILALADGTIFRGLSIGSLGHRVGEVVFNTAMTGYQEILTDPSYARQLVTLTYPHIGNTGTNAEDVESGNGH
QVWAEGLIIRDATMVTSNFRNSESLSDYLQSHNTVAIADIDTRQLTRLLRDKGAQNGCIMTASNGATISSEDEQQAIKLA
QEFAGIEGMDLAKECCTPTTFEWTAGSWDLSDTLLNRDVAGSHDSGTGGFFKHLGTHVEAKYNVVAYDFGSKTNILRMLV
DRGCHVTVVPAQTPIADVLAMNPDGIFLSNGPGDPAACDYAIDAVRHIIEQTDIPTFGICLGHQLIGLASGAKTIKMKTG
HHGANHPVQDLATGTVMITSQNHGFAVDEDTLPANVKSTHRSLFDGTNQGIELTNKPVFSFQGHPEASPGPHDAAPLFDK
FADMMAAAKA

Sequences:

>Translated_410_residues
MNSSAEIQAILALADGTIFRGLSIGSLGHRVGEVVFNTAMTGYQEILTDPSYARQLVTLTYPHIGNTGTNAEDVESGNGH
QVWAEGLIIRDATMVTSNFRNSESLSDYLQSHNTVAIADIDTRQLTRLLRDKGAQNGCIMTASNGATISSEDEQQAIKLA
QEFAGIEGMDLAKECCTPTTFEWTAGSWDLSDTLLNRDVAGSHDSGTGGFFKHLGTHVEAKYNVVAYDFGSKTNILRMLV
DRGCHVTVVPAQTPIADVLAMNPDGIFLSNGPGDPAACDYAIDAVRHIIEQTDIPTFGICLGHQLIGLASGAKTIKMKTG
HHGANHPVQDLATGTVMITSQNHGFAVDEDTLPANVKSTHRSLFDGTNQGIELTNKPVFSFQGHPEASPGPHDAAPLFDK
FADMMAAAKA
>Mature_410_residues
MNSSAEIQAILALADGTIFRGLSIGSLGHRVGEVVFNTAMTGYQEILTDPSYARQLVTLTYPHIGNTGTNAEDVESGNGH
QVWAEGLIIRDATMVTSNFRNSESLSDYLQSHNTVAIADIDTRQLTRLLRDKGAQNGCIMTASNGATISSEDEQQAIKLA
QEFAGIEGMDLAKECCTPTTFEWTAGSWDLSDTLLNRDVAGSHDSGTGGFFKHLGTHVEAKYNVVAYDFGSKTNILRMLV
DRGCHVTVVPAQTPIADVLAMNPDGIFLSNGPGDPAACDYAIDAVRHIIEQTDIPTFGICLGHQLIGLASGAKTIKMKTG
HHGANHPVQDLATGTVMITSQNHGFAVDEDTLPANVKSTHRSLFDGTNQGIELTNKPVFSFQGHPEASPGPHDAAPLFDK
FADMMAAAKA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=412, Percent_Identity=35.6796116504854, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI21361331, Length=411, Percent_Identity=36.7396593673966, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI169790915, Length=411, Percent_Identity=36.7396593673966, Blast_Score=212, Evalue=4e-55,
Organism=Escherichia coli, GI1786215, Length=397, Percent_Identity=63.9798488664987, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI193204318, Length=415, Percent_Identity=37.3493975903614, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=415, Percent_Identity=38.0722891566265, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=414, Percent_Identity=35.5072463768116, Blast_Score=222, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45555749, Length=418, Percent_Identity=37.799043062201, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24642586, Length=418, Percent_Identity=37.799043062201, Blast_Score=234, Evalue=7e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43755; Mature: 43755

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSAEIQAILALADGTIFRGLSIGSLGHRVGEVVFNTAMTGYQEILTDPSYARQLVTLT
CCCCHHEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEE
YPHIGNTGTNAEDVESGNGHQVWAEGLIIRDATMVTSNFRNSESLSDYLQSHNTVAIADI
CCCCCCCCCCHHHCCCCCCCEEEECCEEEECHHHHHHCCCCHHHHHHHHHCCCEEEEEEC
DTRQLTRLLRDKGAQNGCIMTASNGATISSEDEQQAIKLAQEFAGIEGMDLAKECCTPTT
CHHHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
FEWTAGSWDLSDTLLNRDVAGSHDSGTGGFFKHLGTHVEAKYNVVAYDFGSKTNILRMLV
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHH
DRGCHVTVVPAQTPIADVLAMNPDGIFLSNGPGDPAACDYAIDAVRHIIEQTDIPTFGIC
HCCCEEEEEECCCCHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LGHQLIGLASGAKTIKMKTGHHGANHPVQDLATGTVMITSQNHGFAVDEDTLPANVKSTH
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCEEECCCCCCCCHHHHH
RSLFDGTNQGIELTNKPVFSFQGHPEASPGPHDAAPLFDKFADMMAAAKA
HHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSSAEIQAILALADGTIFRGLSIGSLGHRVGEVVFNTAMTGYQEILTDPSYARQLVTLT
CCCCHHEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEE
YPHIGNTGTNAEDVESGNGHQVWAEGLIIRDATMVTSNFRNSESLSDYLQSHNTVAIADI
CCCCCCCCCCHHHCCCCCCCEEEECCEEEECHHHHHHCCCCHHHHHHHHHCCCEEEEEEC
DTRQLTRLLRDKGAQNGCIMTASNGATISSEDEQQAIKLAQEFAGIEGMDLAKECCTPTT
CHHHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
FEWTAGSWDLSDTLLNRDVAGSHDSGTGGFFKHLGTHVEAKYNVVAYDFGSKTNILRMLV
EEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHH
DRGCHVTVVPAQTPIADVLAMNPDGIFLSNGPGDPAACDYAIDAVRHIIEQTDIPTFGIC
HCCCEEEEEECCCCHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
LGHQLIGLASGAKTIKMKTGHHGANHPVQDLATGTVMITSQNHGFAVDEDTLPANVKSTH
HHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCEEECCCCCCCCHHHHH
RSLFDGTNQGIELTNKPVFSFQGHPEASPGPHDAAPLFDKFADMMAAAKA
HHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA